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The map label for this gene is frvX

Identifier: 157163370

GI number: 157163370

Start: 4118048

End: 4119118

Strand: Reverse

Name: frvX

Synonym: EcHS_A4126

Alternate gene names: 157163370

Gene position: 4119118-4118048 (Counterclockwise)

Preceding gene: 157163371

Following gene: 157163369

Centisome position: 88.71

GC content: 52.57

Gene sequence:

>1071_bases
ATGAACATTGAGTTACTGCAACAGTTGTGCGAAGCCAGCGCCGTCAGCGGCGATGAACAGGAAGTTCGCGACATTCTGAT
AAACACGCTGGAACCTTGCGTGAATGAAATCACCTTCGATGGTCTGGGCAGCTTTGTTGCCCGTAAGGGGAATAAAGGTC
CAAAAGTTGCCGTTGTCGGACATATGGATGAAGTCGGCTTTATGGTCACCCACATCGACGAGAGCGGTTTTCTGCGTTTT
ACCACCATTGGCGGCTGGTGGAATCAGTCGATGCTCAACCACCGGGTAACCATACGCACACACAAGGGAGTGAAAATCCC
TGGTGTGATTGGTTCCGTCGCGCCTCATGCGTTAACGGAAAAGCAAAAGCAACAACCGCTGTCATTTGATGAGATGTTCA
TTGATATTGGCGCGAACAGTCGCGAAGAGGTGGAAAAGCGCGGCGTGGAAATTGGTAATTTTATTAGCCCGGAAGCCAAT
TTTGCCTGCTGGGGCGAAGATAAAGTGGTCGGCAAGGCGTTGGATAACCGCATCGGCTGCGCAATGATGGCTGAACTATT
GCAGACGGTGAATAATCCCGAAATTACGCTGTATGGCGTTGGCAGTGTGGAAGAAGAAGTTGGGCTACGCGGGGCGCAAA
CCTCGGCGGAACACATTAAACCGGACGTCGTGATCGTGTTGGATACCGCCGTAGCGGGCGATGTTCCGGGCATTGATAAC
ATTAAATACCCGCTGAAACTGGGCCAGGGGCCGGGGCTGATGCTGTTTGACAAGCGCTACTTCCCCAACCAGAAACTGGT
AGCAGCGTTAAAAAGCTGTGCCGCACATAACGATTTACCGCTGCAATTTTCCACCATGAAAACCGGTGCGACGGATGGCG
GGCGCTACAACGTGATGGGCGGCGGGCGTCCGGTTGTCGCGCTGTGTCTGCCAACTCGTTATCTGCACGCCAACAGCGGT
ATGATTTCAAAAGCCGATTACGAAGCTCTGCTCACGCTGATACGGGGTTTTCTGACGACCTTAACTGCGGAGAAAGTCAA
CGCGTTTAGCCAGTTCCGTCAGGTGGATTAA

Upstream 100 bases:

>100_bases
GTGCTGGCGATTGGCCTCGCGCTGAACATTTCATTCATTATCGTCCTGAAAGGATTGTGGTTACGCCGCAAAGCGAAAGC
CGCGCAACAGGAGCTTGTCC

Downstream 100 bases:

>100_bases
TGCTAAACGAACGCCAGTTAAAGATTGTCGATCTGCTGGAGCAACAGCCGCGCACGCCTGGCGAGCTGGCGCAACAGACT
GGCGTTTCAGGCAGGACCAT

Product: putative fructose-specific phosphotransferase system protein FrvX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGSFVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRF
TTIGGWWNQSMLNHRVTIRTHKGVKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSREEVEKRGVEIGNFISPEAN
FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEHIKPDVVIVLDTAVAGDVPGIDN
IKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAHNDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSG
MISKADYEALLTLIRGFLTTLTAEKVNAFSQFRQVD

Sequences:

>Translated_356_residues
MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGSFVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRF
TTIGGWWNQSMLNHRVTIRTHKGVKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSREEVEKRGVEIGNFISPEAN
FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEHIKPDVVIVLDTAVAGDVPGIDN
IKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAHNDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSG
MISKADYEALLTLIRGFLTTLTAEKVNAFSQFRQVD
>Mature_356_residues
MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGSFVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRF
TTIGGWWNQSMLNHRVTIRTHKGVKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSREEVEKRGVEIGNFISPEAN
FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEHIKPDVVIVLDTAVAGDVPGIDN
IKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAHNDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSG
MISKADYEALLTLIRGFLTTLTAEKVNAFSQFRQVD

Specific function: Unknown

COG id: COG1363

COG function: function code G; Cellulase M and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M42 family

Homologues:

Organism=Escherichia coli, GI1790332, Length=356, Percent_Identity=100, Blast_Score=738, Evalue=0.0,
Organism=Escherichia coli, GI1788727, Length=360, Percent_Identity=34.7222222222222, Blast_Score=198, Evalue=4e-52,
Organism=Escherichia coli, GI87082417, Length=346, Percent_Identity=29.4797687861272, Blast_Score=156, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FRVX_ECOLI (P32153)

Other databases:

- EMBL:   L19201
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   S40842
- RefSeq:   AP_003910.1
- RefSeq:   NP_418334.1
- ProteinModelPortal:   P32153
- SMR:   P32153
- DIP:   DIP-9694N
- STRING:   P32153
- MEROPS:   M42.001
- PRIDE:   P32153
- EnsemblBacteria:   EBESCT00000002995
- EnsemblBacteria:   EBESCT00000014689
- GeneID:   948388
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW3869
- KEGG:   eco:b3898
- EchoBASE:   EB1808
- EcoGene:   EG11862
- eggNOG:   COG1363
- GeneTree:   EBGT00050000008972
- HOGENOM:   HBG426188
- OMA:   NSRDEVE
- ProtClustDB:   PRK09864
- BioCyc:   EcoCyc:EG11862-MONOMER
- Genevestigator:   P32153
- InterPro:   IPR008007
- PIRSF:   PIRSF001123

Pfam domain/function: PF05343 Peptidase_M42

EC number: NA

Molecular weight: Translated: 38734; Mature: 38734

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: ACT_SITE 205-205

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGSFVARKGNKGPKVAVVG
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEE
HMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHKGVKIPGVIGSVAPHALTE
CCCCCCEEEEEECCCCCEEEEEECCCHHHHHHCCEEEEEECCCEECCCHHHHCCCHHHHH
KQKQQPLSFDEMFIDIGANSREEVEKRGVEIGNFISPEANFACWGEDKVVGKALDNRIGC
HHHHCCCCHHHEEEECCCCCHHHHHHCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHH
AMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEHIKPDVVIVLDTAVAGDVPGIDN
HHHHHHHHHCCCCCEEEEECCCCHHHHCCCCCCCCHHHCCCCEEEEEECHHCCCCCCCCC
IKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAHNDLPLQFSTMKTGATDGGRYNVMG
CCCCEEECCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEEECC
GGRPVVALCLPTRYLHANSGMISKADYEALLTLIRGFLTTLTAEKVNAFSQFRQVD
CCCCEEEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGSFVARKGNKGPKVAVVG
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEE
HMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHKGVKIPGVIGSVAPHALTE
CCCCCCEEEEEECCCCCEEEEEECCCHHHHHHCCEEEEEECCCEECCCHHHHCCCHHHHH
KQKQQPLSFDEMFIDIGANSREEVEKRGVEIGNFISPEANFACWGEDKVVGKALDNRIGC
HHHHCCCCHHHEEEECCCCCHHHHHHCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHH
AMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEHIKPDVVIVLDTAVAGDVPGIDN
HHHHHHHHHCCCCCEEEEECCCCHHHHCCCCCCCCHHHCCCCEEEEEECHHCCCCCCCCC
IKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAHNDLPLQFSTMKTGATDGGRYNVMG
CCCCEEECCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEEECC
GGRPVVALCLPTRYLHANSGMISKADYEALLTLIRGFLTTLTAEKVNAFSQFRQVD
CCCCEEEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8346018; 9278503; 8019415