Click here to switch to the map view.

The map label for this gene is hybB

Identifier: 157162475

GI number: 157162475

Start: 3187816

End: 3188994

Strand: Reverse

Name: hybB

Synonym: EcHS_A3175

Alternate gene names: 157162475

Gene position: 3188994-3187816 (Counterclockwise)

Preceding gene: 157162476

Following gene: 157162474

Centisome position: 68.68

GC content: 54.11

Gene sequence:

>1179_bases
ATGAGTCATGATCCACAACCGCTGGGCGGCAAAATCATCAGTAAACCGGTCATGATTTTTGGACCGTTAATCGTCATCTG
TATGCTCCTGATTGTGAAGCGTCTGGTGTTCGGTCTGGGCTCTGTCTCTGACCTGAACGGCGGCTTCCCGTGGGGCGTGT
GGATCGCGTTTGACCTGCTGATTGGCACCGGCTTTGCCTGTGGCGGCTGGGCGCTGGCGTGGGCGGTATACGTCTTTAAC
CGTGGGCAATACCATCCGCTGGTGCGTCCGGCGCTGTTGGCGAGTCTGTTTGGTTACTCACTGGGTGGCTTGTCGATCAC
TATCGACGTGGGTCGCTACTGGAACCTGCCGTACTTCTACATTCCGGGTCACTTCAACGTGAACTCGGTACTGTTCGAGA
CGGCGGTCTGTATGACCATCTATATCGGCGTGATGGCACTGGAGTTTGCTCCGGCACTGTTTGAACGTCTGGGGTGGAAG
GTGTCGCTACAGCGACTAAACAAGGTGATGTTCTTCATCATCGCGCTCGGTGCGCTGCTGCCGACCATGCACCAGTCTTC
AATGGGGTCGCTGATGATCTCGGCGGGCTACAAGGTGCATCCGTTGTGGCAGAGCTATGAAATGTTGCCGCTGTTCTCGC
TGCTGACGGCGTTCATCATGGGCTTCTCGATTGTCATCTTTGAAGGTTCGCTGGTGCAGGCGGGTCTGCGTGGCAACGGT
CCGGATGAAAAGAGTCTGTTTGTTAAGCTGACCAACACCATCAGTGTGTTGCTGGCGATTTTCATCGTGCTGCGCTTTGG
CGAGCTTATCTATCGCGACAAGCTGTCGTTAGCGTTTGCCGGTGACTTCTACTCCGTGATGTTCTGGATTGAAGTCCTGC
TGATGCTCTTCCCGCTGGTCGTTCTGCGTGTGGCGAAGCTGCGTAATGATTCCCGCATGCTGTTCCTGTCAGCACTGAGC
GCACTGTTAGGTTGTGCAACCTGGCGTCTGACCTATTCGCTGGTGGCATTCAACCCGGGCGGCGGTTACGCCTACTTCCC
GACCTGGGAAGAACTGTTGATTTCTATTGGTTTTGTGGCTATTGAGATTTGCGCTTACATCGTACTCATTCGTCTACTGC
CGATACTTCCTCCTTTAAAACAAAACGATCATAATCGTCATGAGGCGAGCAAAGCATGA

Upstream 100 bases:

>100_bases
CTGTATAAAGGCATGATGCTACCACTGGCTGTGCTGGCGGGCTTAACCGTGCTGGTTCGTCGCAACACCAAAAACGACCA
TCACGACGGAGGAGACGATC

Downstream 100 bases:

>100_bases
GCCAGAGAATTACTATTGATCCGGTAACCCGTATTGAGGGGCATTTACGCATCGATTGCGAAATCGAAAATGGCGTCGTT
TCGAAAGCATGGGCTTCCGG

Product: putative hydrogenase 2 b cytochrome subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSDLNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFN
RGQYHPLVRPALLASLFGYSLGGLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAFIMGFSIVIFEGSLVQAGLRGNG
PDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLSLAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALS
ALLGCATWRLTYSLVAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA

Sequences:

>Translated_392_residues
MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSDLNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFN
RGQYHPLVRPALLASLFGYSLGGLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAFIMGFSIVIFEGSLVQAGLRGNG
PDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLSLAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALS
ALLGCATWRLTYSLVAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
>Mature_391_residues
SHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSDLNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNR
GQYHPLVRPALLASLFGYSLGGLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWKV
SLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAFIMGFSIVIFEGSLVQAGLRGNGP
DEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLSLAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSA
LLGCATWRLTYSLVAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA

Specific function: Probable b-type cytochrome

COG id: COG5557

COG function: function code C; Polysulphide reductase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nrfD family

Homologues:

Organism=Escherichia coli, GI2367183, Length=392, Percent_Identity=100, Blast_Score=785, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HYBB_ECOLI (P37180)

Other databases:

- EMBL:   U09177
- EMBL:   U28377
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   A65086
- RefSeq:   AP_003550.1
- RefSeq:   NP_417469.1
- ProteinModelPortal:   P37180
- STRING:   P37180
- EnsemblBacteria:   EBESCT00000003735
- EnsemblBacteria:   EBESCT00000018135
- GeneID:   948615
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW5494
- KEGG:   eco:b2995
- EchoBASE:   EB1748
- EcoGene:   EG11800
- eggNOG:   COG5557
- GeneTree:   EBGT00050000009358
- HOGENOM:   HBG457068
- OMA:   MEETIMS
- ProtClustDB:   PRK10881
- BioCyc:   EcoCyc:EG11800-MONOMER
- BioCyc:   MetaCyc:EG11800-MONOMER
- Genevestigator:   P37180
- GO:   GO:0006810
- InterPro:   IPR005614

Pfam domain/function: PF03916 NrfD

EC number: NA

Molecular weight: Translated: 43603; Mature: 43471

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1747e03c)-; HASH(0x125377b0)-; HASH(0x174346ac)-; HASH(0x175a62b0)-; HASH(0x1742ed04)-; HASH(0x16dc9b9c)-; HASH(0x16fdd344)-; HASH(0x172d3770)-; HASH(0x17420a94)-; HASH(0x17446370)-; HASH(0x171c8208)-;

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSDLNGGFPWGVWIAFDLL
CCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
IGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLGGLSITIDVGRYWNLPYFY
HHCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCEECCCEEE
IPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWKVSLQRLNKVMFFIIALGALL
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
PTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAFIMGFSIVIFEGSLVQAGLRGNG
HHHHHHHHCCEEEECCCEEEEHHHCHHHHHHHHHHHHHHHHHHHEEEECHHHCCCCCCCC
PDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLSLAFAGDFYSVMFWIEVLLMLFPLV
CCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHH
VLRVAKLRNDSRMLFLSALSALLGCATWRLTYSLVAFNPGGGYAYFPTWEELLISIGFVA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCEEECCCHHHHHHHHHHHH
IEICAYIVLIRLLPILPPLKQNDHNRHEASKA
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCH
>Mature Secondary Structure 
SHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSDLNGGFPWGVWIAFDLL
CCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
IGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLGGLSITIDVGRYWNLPYFY
HHCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCEECCCEEE
IPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWKVSLQRLNKVMFFIIALGALL
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
PTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAFIMGFSIVIFEGSLVQAGLRGNG
HHHHHHHHCCEEEECCCEEEEHHHCHHHHHHHHHHHHHHHHHHHEEEECHHHCCCCCCCC
PDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLSLAFAGDFYSVMFWIEVLLMLFPLV
CCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHH
VLRVAKLRNDSRMLFLSALSALLGCATWRLTYSLVAFNPGGGYAYFPTWEELLISIGFVA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCEEECCCHHHHHHHHHHHH
IEICAYIVLIRLLPILPPLKQNDHNRHEASKA
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8021226; 9278503