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The map label for this gene is hybA [H]

Identifier: 157162476

GI number: 157162476

Start: 3188984

End: 3189970

Strand: Reverse

Name: hybA [H]

Synonym: EcHS_A3176

Alternate gene names: 157162476

Gene position: 3189970-3188984 (Counterclockwise)

Preceding gene: 157162477

Following gene: 157162475

Centisome position: 68.7

GC content: 54.51

Gene sequence:

>987_bases
GTGAACAGACGTAATTTTATTAAAGCAGCCTCCTGCGGGGCATTGCTGACGGGCGCGCTGCCGTCTGTCAGTCATGCGGC
TGCTGAAAACCGCCCGCCAATTCCGGGATCGCTGGGGATGTTGTACGACTCGACCTTGTGCGTAGGCTGCCAGGCTTGCG
TCACCAAGTGTCAGGATATCAATTTCCCTGAACGTAACCCGCAAGGGGAACAGATCTGGTCGAACAACGACAAACTGTCG
CCGTATACCAATAACATCATTCAGGTGTGGACCAGCGGCACAGGGGTCAACAAAGACCAGGAGGAGAACGGCTACGCGTA
CATTAAGAAACAGTGTATGCACTGCGTCGATCCGAACTGTGTCTCTGTGTGCCCGGTCTCTGCACTGAAAAAAGATCCGA
AAACCGGCATTGTCCATTACGACAAAGACGTGTGCACCGGCTGCCGTTACTGCATGGTCGCCTGTCCGTACAACGTGCCG
AAGTACGACTACAACAACCCGTTTGGTGCGCTGCATAAGTGCGAGCTGTGCAACCAGAAAGGTGTGGAACGTCTCGATAA
AGGCGGTCTACCTGGCTGCGTAGAAGTGTGCCCGGCGGGCGCGGTGATTTTCGGTACGCGTGAAGAGCTGATGGCGGAGG
CGAAAAAACGTCTGGCGCTGAAGCCTGGCAGCGAATACCACTATCCGCGTCAGACGCTGAAATCTGGCGACACTTACCTG
CATACGGTGCCGAAATATTATCCGCATCTGTACGGCGAGAAAGAGGGCGGCGGTACTCAGGTTCTGGTACTGACGGGTGT
GCCTTATGAAAATCTCGACCTGCCGAAACTGGACGATCTTTCTACCGGTGCGCGTTCCGAAAATATTCAACACACCCTGT
ATAAAGGCATGATGCTACCACTGGCTGTGCTGGCGGGCTTAACCGTGCTGGTTCGTCGCAACACCAAAAACGACCATCAC
GACGGAGGAGACGATCATGAGTCATGA

Upstream 100 bases:

>100_bases
GCGGTGTGGTTGGGTTGGTTGCCGGTGTCAGCGTGATGGCGGTGCGTGAACTGGGTCGTCAGCAAAAGAAAGATAACGCT
GACTCACGGGGAGAATAACC

Downstream 100 bases:

>100_bases
TCCACAACCGCTGGGCGGCAAAATCATCAGTAAACCGGTCATGATTTTTGGACCGTTAATCGTCATCTGTATGCTCCTGA
TTGTGAAGCGTCTGGTGTTC

Product: hydrogenase 2 protein HybA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDSTLCVGCQACVTKCQDINFPERNPQGEQIWSNNDKLS
PYTNNIIQVWTSGTGVNKDQEENGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRLALKPGSEYHYPRQTLKSGDTYL
HTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPKLDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHH
DGGDDHES

Sequences:

>Translated_328_residues
MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDSTLCVGCQACVTKCQDINFPERNPQGEQIWSNNDKLS
PYTNNIIQVWTSGTGVNKDQEENGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRLALKPGSEYHYPRQTLKSGDTYL
HTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPKLDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHH
DGGDDHES
>Mature_328_residues
MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDSTLCVGCQACVTKCQDINFPERNPQGEQIWSNNDKLS
PYTNNIIQVWTSGTGVNKDQEENGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRLALKPGSEYHYPRQTLKSGDTYL
HTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPKLDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHH
DGGDDHES

Specific function: Participates in the periplasmic electron-transferring activity of hydrogenase 2 during its catalytic turnover [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789370, Length=328, Percent_Identity=99.6951219512195, Blast_Score=679, Evalue=0.0,
Organism=Escherichia coli, GI2367345, Length=255, Percent_Identity=30.1960784313725, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1787749, Length=283, Percent_Identity=27.5618374558304, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1790326, Length=185, Percent_Identity=34.0540540540541, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787872, Length=196, Percent_Identity=34.6938775510204, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787122, Length=198, Percent_Identity=33.8383838383838, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI226510944, Length=215, Percent_Identity=30.6976744186047, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1787478, Length=133, Percent_Identity=33.8345864661654, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787740, Length=99, Percent_Identity=37.3737373737374, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87082180, Length=192, Percent_Identity=29.1666666666667, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 36015; Mature: 36015

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
5.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDSTLCVGCQACVTKCQDI
CCCCHHHHHHHCCHHHHCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
NFPERNPQGEQIWSNNDKLSPYTNNIIQVWTSGTGVNKDQEENGYAYIKKQCMHCVDPNC
CCCCCCCCCCHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCC
VSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVPKYDYNNPFGALHKCELCNQK
HHCCCHHHHHCCCCCCCEEECHHHHHHHHHEEEECCCCCCCCCCCCCCCHHHHHHHHHHH
GVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRLALKPGSEYHYPRQTLKSGDTYL
HHHHHHCCCCCHHHHHCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCEE
HTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPKLDDLSTGARSENIQHTLYKGMMLP
EHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
LAVLAGLTVLVRRNTKNDHHDGGDDHES
HHHHHHHHEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDSTLCVGCQACVTKCQDI
CCCCHHHHHHHCCHHHHCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
NFPERNPQGEQIWSNNDKLSPYTNNIIQVWTSGTGVNKDQEENGYAYIKKQCMHCVDPNC
CCCCCCCCCCHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCC
VSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVPKYDYNNPFGALHKCELCNQK
HHCCCHHHHHCCCCCCCEEECHHHHHHHHHEEEECCCCCCCCCCCCCCCHHHHHHHHHHH
GVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRLALKPGSEYHYPRQTLKSGDTYL
HHHHHHCCCCCHHHHHCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCEE
HTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPKLDDLSTGARSENIQHTLYKGMMLP
EHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
LAVLAGLTVLVRRNTKNDHHDGGDDHES
HHHHHHHHEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]