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The map label for this gene is hypE

Identifier: 157162177

GI number: 157162177

Start: 2870672

End: 2871682

Strand: Direct

Name: hypE

Synonym: EcHS_A2867

Alternate gene names: 157162177

Gene position: 2870672-2871682 (Clockwise)

Preceding gene: 157162176

Following gene: 157162178

Centisome position: 61.82

GC content: 57.27

Gene sequence:

>1011_bases
GTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTT
TGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGG
CGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCAAGCTGGCGATTTGCGGCACA
GCCAATGACGTTGCGGTCAGTGGCGCTATTCCGCGCTATCTCTCCTGTGGCTTTATCCTCGAAGAAGGATTGCCGATGGA
GACACTGAAAGCCGTAGTGACCAGCATGGCAGAAACCGCCCGCGCGGCAGGCATTGCCATCGTTACTGGCGATACTAAAG
TGGTGCAGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGC
GCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTAACCTGCG
TGAGCAGCTGGGGCTGGATGGCGAACTGGTCAGCGACTGCGCGGTGCTGACGCCGCTTATTCAGACGCTGCGTGACATTC
CCGGCGTGAAAGCGCTGCGTGATGCCACCCGTGGTGGTGTAAACGCGGTGGTTCATGAGTTCGCGGCAGCCTGCGGTTGT
GGTATTGAACTTTCAGAAGCGGCACTGCCTGTTAAACCTGCCGTGCGTGGCGTTTGCGAATTGCTGGGACTGGACGCCCT
GAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCC
ATCCACTGGGGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTG
AAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAA

Upstream 100 bases:

>100_bases
GTAACACCTGTAATCCTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGTGGTATCAGTAT
CGTCAGCAGGAGAGTGAAGC

Downstream 100 bases:

>100_bases
TAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCAT
ATACACCGATGAGTGATCTC

Product: hydrogenase expression/formation protein HypE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLAQLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGT
ANDVAVSGAIPRYLSCGFILEEGLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAVDKLFINTAGMGAIPANIHWG
AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKALRDATRGGVNAVVHEFAAACGC
GIELSEAALPVKPAVRGVCELLGLDALNFANEGKLVIAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGV
KRTLDLPHAEPLPRIC

Sequences:

>Translated_336_residues
MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLAQLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGT
ANDVAVSGAIPRYLSCGFILEEGLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAVDKLFINTAGMGAIPANIHWG
AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKALRDATRGGVNAVVHEFAAACGC
GIELSEAALPVKPAVRGVCELLGLDALNFANEGKLVIAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGV
KRTLDLPHAEPLPRIC
>Mature_336_residues
MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLAQLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGT
ANDVAVSGAIPRYLSCGFILEEGLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAVDKLFINTAGMGAIPANIHWG
AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKALRDATRGGVNAVVHEFAAACGC
GIELSEAALPVKPAVRGVCELLGLDALNFANEGKLVIAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGV
KRTLDLPHAEPLPRIC

Specific function: Unknown

COG id: COG0309

COG function: function code O; Hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hypE family

Homologues:

Organism=Escherichia coli, GI226510967, Length=336, Percent_Identity=100, Blast_Score=674, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HYPE_ECOLI (P24193)

Other databases:

- EMBL:   X54543
- EMBL:   U29579
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   S15201
- RefSeq:   AP_003297.1
- RefSeq:   NP_417210.2
- PDB:   2I6R
- PDBsum:   2I6R
- ProteinModelPortal:   P24193
- SMR:   P24193
- DIP:   DIP-9999N
- IntAct:   P24193
- MINT:   MINT-1239514
- STRING:   P24193
- EnsemblBacteria:   EBESCT00000003460
- EnsemblBacteria:   EBESCT00000003461
- EnsemblBacteria:   EBESCT00000003462
- EnsemblBacteria:   EBESCT00000003463
- EnsemblBacteria:   EBESCT00000016316
- GeneID:   947182
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW2700
- KEGG:   eco:b2730
- EchoBASE:   EB0482
- EcoGene:   EG10487
- eggNOG:   COG0309
- GeneTree:   EBGT00050000011488
- HOGENOM:   HBG287946
- ProtClustDB:   CLSK880468
- BioCyc:   EcoCyc:EG10487-MONOMER
- Genevestigator:   P24193
- InterPro:   IPR000728
- InterPro:   IPR010918
- InterPro:   IPR011854
- InterPro:   IPR016188
- PIRSF:   PIRSF005644
- TIGRFAMs:   TIGR02124

Pfam domain/function: PF00586 AIRS; PF02769 AIRS_C; SSF56042 AIR_synth_C; SSF55326 PurM_N-like

EC number: NA

Molecular weight: Translated: 35093; Mature: 35093

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLAQLVAEGDRLAFSTDSY
CCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCE
VIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEEGLPMETLKAVVTSMAETA
EECEEECCCCCCCEEEEECCCCCEEECCCCHHHHHCCHHHCCCCCHHHHHHHHHHHHHHH
RAAGIAIVTGDTKVVQRGAVDKLFINTAGMGAIPANIHWGAQTLTAGDVLLVSGTLGDHG
HHCCEEEEECCHHHHHCCCCCEEEEECCCCCCCCCCEECCCCEECCCCEEEEECCCCCCC
ATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKALRDATRGGVNAVVHEFAAACGC
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHCC
GIELSEAALPVKPAVRGVCELLGLDALNFANEGKLVIAVERNAAEQVLAALHSHPLGKDA
CCCCCCCCCCCCHHHHHHHHHHCCHHHCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCHH
ALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPRIC
HHHHHHHHHCCCEEEEEHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLAQLVAEGDRLAFSTDSY
CCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCE
VIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEEGLPMETLKAVVTSMAETA
EECEEECCCCCCCEEEEECCCCCEEECCCCHHHHHCCHHHCCCCCHHHHHHHHHHHHHHH
RAAGIAIVTGDTKVVQRGAVDKLFINTAGMGAIPANIHWGAQTLTAGDVLLVSGTLGDHG
HHCCEEEEECCHHHHHCCCCCEEEEECCCCCCCCCCEECCCCEECCCCEEEEECCCCCCC
ATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKALRDATRGGVNAVVHEFAAACGC
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHCC
GIELSEAALPVKPAVRGVCELLGLDALNFANEGKLVIAVERNAAEQVLAALHSHPLGKDA
CCCCCCCCCCCCHHHHHHHHHHCCHHHCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCHH
ALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPRIC
HHHHHHHHHCCCEEEEEHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1849603; 9278503