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The map label for this gene is fhlA

Identifier: 157162178

GI number: 157162178

Start: 2871756

End: 2873834

Strand: Direct

Name: fhlA

Synonym: EcHS_A2868

Alternate gene names: 157162178

Gene position: 2871756-2873834 (Clockwise)

Preceding gene: 157162177

Following gene: 157162181

Centisome position: 61.84

GC content: 52.57

Gene sequence:

>2079_bases
ATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGACACTATTGCAGCAGCCCGATCT
GGCCTCGCTGTGTGAGGCTCTTTCGCAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCAAG
CGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCA
CACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCA
GCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTG
GCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTT
TCTGTCGTCACCGAACAAATCCAGAGCCGCGTCGTTAACAATGTCGACTATGAGTTGTTATGCCGGGAACGCGATAACTT
CCGCATCCTGGTCGCCATCACCAACGCGGTGCTTTCCCGCCTGGATATGGACGAACTGGTCAGCGAAGTCGCCAAAGAAA
TCCATTACTATTTCGACATTGACGATATCAGTATCGTCTTACGCAGCCACCGTAAAAACAAACTCAACATCTACTCCACT
CACTATCTTGATAAACAGCATCCCGCCCACGAACAGAGCGAAGTCGATGAAGCCGGAACCCTCACCGAACGCGTGTTCAA
AAGTAAAGAGATGCTGCTGATCAATCTCCACGAGCGGGACGATTTAGCCCCCTATGAACGCATGTTGTTCGACACCTGGG
GCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGC
GAAGAGAAAGTGTTTACCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGC
CCTCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATG
TTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGTGCTTAAACAAGTTGAAATGGTGGCGCAA
AGTGACAGTACCGTGCTGATCCTCGGTGAAACTGGCACGGGTAAAGAGCTGATTGCCCGTGCGATCCATAATCTCAGTGG
GCGTAATAATCGCCGCATGGTCAAAATGAACTGCGCGGCGATGCCTGCCGGATTGCTGGAAAGCGATCTGTTTGGTCATG
AGCGTGGGGCTTTTACCGGTGCCAGCGCCCAGCGTATCGGTCGTTTTGAACTGGCGGATAAAAGCTCCCTGTTCCTCGAC
GAAGTGGGCGATATGCCACTGGAGTTACAGCCGAAGTTGCTGCGTGTATTGCAGGAACAGGAGTTTGAACGTCTCGGCAG
CAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCGATCTGAAAAAAATGGTCGCCGACCGTGAGT
TCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACCTGCCGCCACTACGCGAGCGTCCGGAAGATATTCCG
CTGCTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGCG
CACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCG
GTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTG
GAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGC
GCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAA

Upstream 100 bases:

>100_bases
GCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGG
TGCTTTCCTGGAAGAACAAA

Downstream 100 bases:

>100_bases
CTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTG
GCACCGGACGCTTGTCATCG

Product: formate hydrogenlyase transcriptional activator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 692; Mature: 691

Protein sequence:

>692_residues
MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALADNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLA
HGPVRSILSRPDTLHCSYEEFCETWPQLDAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYFDIDDISIVLRSHRKNKLNIYST
HYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINLHERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQC
EEKVFTTTNLNLLRQIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVLKQVEMVAQ
SDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDLFGHERGAFTGASAQRIGRFELADKSSLFLD
EVGDMPLELQPKLLRVLQEQEFERLGSNKIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIP
LLAKAFTFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDIVLPEPETPPAATVVAL
EGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMKRLGIDKSALI

Sequences:

>Translated_692_residues
MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALADNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLA
HGPVRSILSRPDTLHCSYEEFCETWPQLDAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYFDIDDISIVLRSHRKNKLNIYST
HYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINLHERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQC
EEKVFTTTNLNLLRQIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVLKQVEMVAQ
SDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDLFGHERGAFTGASAQRIGRFELADKSSLFLD
EVGDMPLELQPKLLRVLQEQEFERLGSNKIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIP
LLAKAFTFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDIVLPEPETPPAATVVAL
EGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMKRLGIDKSALI
>Mature_691_residues
SYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALADNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAH
GPVRSILSRPDTLHCSYEEFCETWPQLDAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIVS
VVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYFDIDDISIVLRSHRKNKLNIYSTH
YLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINLHERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCE
EKVFTTTNLNLLRQIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVLKQVEMVAQS
DSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDLFGHERGAFTGASAQRIGRFELADKSSLFLDE
VGDMPLELQPKLLRVLQEQEFERLGSNKIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPL
LAKAFTFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDIVLPEPETPPAATVVALE
GEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMKRLGIDKSALI

Specific function: Required for induction of expression of the formate dehydrogenase H and hydrogenase-3 structural genes

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain

Homologues:

Organism=Escherichia coli, GI1789087, Length=692, Percent_Identity=100, Blast_Score=1426, Evalue=0.0,
Organism=Escherichia coli, GI87082117, Length=573, Percent_Identity=50.7853403141361, Blast_Score=520, Evalue=1e-148,
Organism=Escherichia coli, GI1790437, Length=313, Percent_Identity=46.9648562300319, Blast_Score=271, Evalue=1e-73,
Organism=Escherichia coli, GI1788550, Length=322, Percent_Identity=46.2732919254658, Blast_Score=267, Evalue=2e-72,
Organism=Escherichia coli, GI1788905, Length=307, Percent_Identity=46.5798045602606, Blast_Score=253, Evalue=4e-68,
Organism=Escherichia coli, GI87082152, Length=363, Percent_Identity=38.8429752066116, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI1790299, Length=367, Percent_Identity=40.3269754768392, Blast_Score=237, Evalue=2e-63,
Organism=Escherichia coli, GI1789233, Length=321, Percent_Identity=42.3676012461059, Blast_Score=236, Evalue=5e-63,
Organism=Escherichia coli, GI87081872, Length=265, Percent_Identity=45.2830188679245, Blast_Score=213, Evalue=4e-56,
Organism=Escherichia coli, GI1786524, Length=332, Percent_Identity=38.2530120481928, Blast_Score=198, Evalue=1e-51,
Organism=Escherichia coli, GI1787583, Length=333, Percent_Identity=36.6366366366366, Blast_Score=191, Evalue=2e-49,
Organism=Escherichia coli, GI1789828, Length=287, Percent_Identity=34.4947735191638, Blast_Score=142, Evalue=9e-35,
Organism=Escherichia coli, GI87081858, Length=326, Percent_Identity=31.9018404907975, Blast_Score=140, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FHLA_ECOLI (P19323)

Other databases:

- EMBL:   X52227
- EMBL:   U29579
- EMBL:   M58504
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   S12079
- RefSeq:   AP_003298.1
- RefSeq:   NP_417211.1
- ProteinModelPortal:   P19323
- IntAct:   P19323
- STRING:   P19323
- EnsemblBacteria:   EBESCT00000000605
- EnsemblBacteria:   EBESCT00000000606
- EnsemblBacteria:   EBESCT00000016661
- GeneID:   947181
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW2701
- KEGG:   eco:b2731
- EchoBASE:   EB0297
- EcoGene:   EG10301
- eggNOG:   COG3604
- GeneTree:   EBGT00050000008655
- HOGENOM:   HBG740884
- OMA:   GHYCLLP
- ProtClustDB:   PRK15429
- BioCyc:   EcoCyc:PD02936
- Genevestigator:   P19323
- GO:   GO:0005622
- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- PRINTS:   PR01590
- SMART:   SM00382
- SMART:   SM00065

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat; SSF46689 Homeodomain_like

EC number: NA

Molecular weight: Translated: 78469; Mature: 78338

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00675 SIGMA54_INTERACT_1; PS00676 SIGMA54_INTERACT_2; PS00688 SIGMA54_INTERACT_3; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALADNAAIVLWQAQTQRAS
CCCCCHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHH
YYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFCETWPQLDAGGLYPKFGHY
HHHCCCCCCCCCCCCCEEEECCCHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCE
CLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIVSVVTEQIQSRVVNNVDYELL
EEEEECCCCCEECCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
CRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYFDIDDISIVLRSHRKNKLNIYST
EECCCCCEEEEEEHHHHHHHCCHHHHHHHHHHHHHEEECCHHHHHHHHHCCCCCEEEEEE
HYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINLHERDDLAPYERMLFDTWGNQIQTL
ECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
CLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLRQIAERVAIAVDNALAYQEIHRLKERL
HHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDENLALTEQLNNVDSEFGEIIGRSEAMYSVLKQVEMVAQSDSTVLILGETGTGKELIAR
HCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHH
AIHNLSGRNNRRMVKMNCAAMPAGLLESDLFGHERGAFTGASAQRIGRFELADKSSLFLD
HHHHCCCCCCCEEEEEECHHCCCHHHHHHHCCCCCCCCCCCCHHHCCCEEECCCCHHHHH
EVGDMPLELQPKLLRVLQEQEFERLGSNKIIQTDVRLIAATNRDLKKMVADREFRSDLYY
HHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEHHHHEEEECCHHHHHHHHHHHHHHCCEE
RLNVFPIHLPPLRERPEDIPLLAKAFTFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRE
EEEEEEEECCCHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCHHH
LENVIERAVLLTRGNVLQLSLPDIVLPEPETPPAATVVALEGEDEYQLIVRVLKETNGVV
HHHHHHHHHHHCCCCEEEEECCCEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCEE
AGPKGAAQRLGLKRTTLLSRMKRLGIDKSALI
ECCCHHHHHCCCHHHHHHHHHHHCCCCHHHCC
>Mature Secondary Structure 
SYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALADNAAIVLWQAQTQRAS
CCCCHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHH
YYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFCETWPQLDAGGLYPKFGHY
HHHCCCCCCCCCCCCCEEEECCCHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCE
CLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIVSVVTEQIQSRVVNNVDYELL
EEEEECCCCCEECCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
CRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYFDIDDISIVLRSHRKNKLNIYST
EECCCCCEEEEEEHHHHHHHCCHHHHHHHHHHHHHEEECCHHHHHHHHHCCCCCEEEEEE
HYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINLHERDDLAPYERMLFDTWGNQIQTL
ECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
CLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLRQIAERVAIAVDNALAYQEIHRLKERL
HHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDENLALTEQLNNVDSEFGEIIGRSEAMYSVLKQVEMVAQSDSTVLILGETGTGKELIAR
HCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHH
AIHNLSGRNNRRMVKMNCAAMPAGLLESDLFGHERGAFTGASAQRIGRFELADKSSLFLD
HHHHCCCCCCCEEEEEECHHCCCHHHHHHHCCCCCCCCCCCCHHHCCCEEECCCCHHHHH
EVGDMPLELQPKLLRVLQEQEFERLGSNKIIQTDVRLIAATNRDLKKMVADREFRSDLYY
HHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEHHHHEEEECCHHHHHHHHHHHHHHCCEE
RLNVFPIHLPPLRERPEDIPLLAKAFTFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRE
EEEEEEEECCCHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCHHH
LENVIERAVLLTRGNVLQLSLPDIVLPEPETPPAATVVALEGEDEYQLIVRVLKETNGVV
HHHHHHHHHHHCCCCEEEEECCCEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCEE
AGPKGAAQRLGLKRTTLLSRMKRLGIDKSALI
ECCCHHHHHCCCHHHHHHHHHHHCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2280686; 2118503; 9278503