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The map label for this gene is hycE

Identifier: 157162167

GI number: 157162167

Start: 2862180

End: 2863889

Strand: Reverse

Name: hycE

Synonym: EcHS_A2857

Alternate gene names: 157162167

Gene position: 2863889-2862180 (Counterclockwise)

Preceding gene: 157162168

Following gene: 157162166

Centisome position: 61.67

GC content: 56.9

Gene sequence:

>1710_bases
ATGTCTGAAGAAAAATTAGGTCAACATTATCTCGCCGCGCTGAATGAGGCATTTCCGGGCGTCGTGCTGGACCACGCCTG
GCAGACCAAAGATCAGCTGACTGTCACCGTAAAGGTGAACTACCTGCCGGAAGTGGTGGAGTTTCTTTACTACAAACAGG
GTGGCTGGCTGTCGGTGCTGTTTGGTAACGACGAACGCAAACTGAATGGTCATTACGCCGTTTACTACGTGCTGTCGATG
GAGAAGGGCACTAAGTGTTGGATTACGGTTCGCGTCGAAGTTGACGCCAACAAACCGGAATATCCGTCCGTGACGCCGCG
CGTTCCGGCGGCGGTGTGGGGCGAGCGTGAAGTGCGCGATATGTACGGTTTGATTCCGGTTGGTCTGCCGGATGAACGTC
GTCTGGTGCTGCCGGATGACTGGCCGGATGAACTTTATCCGCTGCGTAAAGACAGCATGGATTATCGTCAGCGTCCGGCA
CCGACCACCGATGCTGAAACCTACGAGTTCATCAACGAACTGGGCGACAAGAAAAACAACGTCGTGCCGATTGGTCCGCT
GCACGTCACTTCTGATGAACCGGGCCACTTCCGTCTGTTCGTCGATGGCGAAAACATTATCGACGCCGACTACCGTCTGT
TCTACGTCCATCGCGGCATGGAAAAACTGGCGGAAACCCGTATGGGTTATAACGAAGTGACCTTCCTCTCTGACCGTGTG
TGCGGGATCTGCGGCTTTGCCCACAGCACCGCCTACACCACGTCGGTGGAAAACGCGATGGGTATTCAGGTGCCAGAACG
TGCGCAGATGATCCGCGCCATTCTGCTGGAGGTAGAACGCTTGCACTCGCATCTGCTCAACCTTGGCCTGGCCTGTCACT
TTACCGGCTTCGACTCCGGCTTTATGCAGTTCTTCCGCGTGCGTGAAACCTCCATGAAAATGGCAGAGATCCTTACCGGT
GCGCGTAAAACCTACGGCCTGAACTTGATCGGCGGGATTCGTCGCGATCTGCTGAAAGACGACATGATCCAGACCCGCCA
GCTGGCACAACAGATGCGTCGTGAAGTGCAGGAGCTGGTGGATGTGCTGCTGAGCACTCCGAACATGGAACAGCGCACTG
TCGGCATTGGTCGTCTGGACCCGGAAATCGCTCGCGACTTCAGTAACGTCGGCCCGATGGTCCGTGCCAGCGGTCACGCC
CGTGATACCCGCGCCGATCACCCGTTTGTCGGCTATGGCCTGCTGCCAATGGAAGTCCACAGCGAGCAGGGCTGCGACGT
TATTTCCCGTCTGAAAGTGCGTATCAACGAAGTCTATACCGCGCTGAACATGATCGACTACGGTCTGGATAACCTGCCGG
GTGGCCCACTGATGGTGGAAGGCTTTACCTACATTCCGCACCGCTTTGCGCTGGGCTTTGCCGAAGCGCCGCGCGGCGAT
GATATCCACTGGAGCATGACCGGCGACAACCAGAAGCTGTACCGCTGGCGCTGCCGTGCCGCGACCTACGCGAACTGGCC
GACCCTGCGCTACATGCTGCGCGGCAACACCGTTTCCGATGCGCCGCTGATTATCGGTAGCCTCGACCCTTGCTACTCCT
GTACCGACCGCATGACCGTGGTCGATGTGCGTAAGAAGAAGAGCAAAGTGGTGCCGTACAAAGAACTCGAGCGTTACAGC
ATTGAGCGTAAAAACTCGCCGCTGAAATAA

Upstream 100 bases:

>100_bases
TGCGTCTTGATATTACTCCGCGCATTACCTGGGCTGGGTTTGGCTTTGCATTTTTAGCGTTCGTCTCCTTGCTGGCGGCG
TGATTAAAGAGAGTTTGAGC

Downstream 100 bases:

>100_bases
GGAATCGCCATGTTTACCTTTATCAAAAAAGTCATCAAAACCGGCACGGCGACCTCGTCTTATCCGCTGGAGCCGATTGC
GGTTGATAAAAACTTCCGTG

Product: formate hydrogenlyase, subunit E

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 5; Hydrogenase-3 component E

Number of amino acids: Translated: 569; Mature: 568

Protein sequence:

>569_residues
MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPEVVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSM
EKGTKCWITVRVEVDANKPEYPSVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEKLAETRMGYNEVTFLSDRV
CGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTG
ARKTYGLNLIGGIRRDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGPMVRASGHA
RDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPGGPLMVEGFTYIPHRFALGFAEAPRGD
DIHWSMTGDNQKLYRWRCRAATYANWPTLRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYS
IERKNSPLK

Sequences:

>Translated_569_residues
MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPEVVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSM
EKGTKCWITVRVEVDANKPEYPSVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEKLAETRMGYNEVTFLSDRV
CGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTG
ARKTYGLNLIGGIRRDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGPMVRASGHA
RDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPGGPLMVEGFTYIPHRFALGFAEAPRGD
DIHWSMTGDNQKLYRWRCRAATYANWPTLRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYS
IERKNSPLK
>Mature_568_residues
SEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPEVVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSME
KGTKCWITVRVEVDANKPEYPSVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPAP
TTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEKLAETRMGYNEVTFLSDRVC
GICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGA
RKTYGLNLIGGIRRDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGPMVRASGHAR
DTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPGGPLMVEGFTYIPHRFALGFAEAPRGDD
IHWSMTGDNQKLYRWRCRAATYANWPTLRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSI
ERKNSPLK

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 49 kDa subunit family

Homologues:

Organism=Homo sapiens, GI4758786, Length=387, Percent_Identity=25.5813953488372, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI260898743, Length=377, Percent_Identity=25.4641909814324, Blast_Score=110, Evalue=4e-24,
Organism=Escherichia coli, GI1789076, Length=569, Percent_Identity=100, Blast_Score=1185, Evalue=0.0,
Organism=Escherichia coli, GI1788832, Length=568, Percent_Identity=70.4225352112676, Blast_Score=858, Evalue=0.0,
Organism=Escherichia coli, GI145693162, Length=563, Percent_Identity=25.7548845470693, Blast_Score=159, Evalue=6e-40,
Organism=Caenorhabditis elegans, GI17568379, Length=387, Percent_Identity=25.5813953488372, Blast_Score=126, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17555284, Length=400, Percent_Identity=25.25, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI221459469, Length=387, Percent_Identity=25.5813953488372, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24638644, Length=387, Percent_Identity=26.6149870801034, Blast_Score=136, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HYCE_ECOLI (P16431)

Other databases:

- EMBL:   X17506
- EMBL:   U29579
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   S08623
- RefSeq:   AP_003288.1
- RefSeq:   NP_417201.1
- ProteinModelPortal:   P16431
- SMR:   P16431
- DIP:   DIP-9975N
- MINT:   MINT-1223046
- STRING:   P16431
- EnsemblBacteria:   EBESCT00000001842
- EnsemblBacteria:   EBESCT00000016299
- GeneID:   947396
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW2691
- KEGG:   eco:b2721
- EchoBASE:   EB0473
- EcoGene:   EG10478
- eggNOG:   COG3261
- GeneTree:   EBGT00050000010529
- HOGENOM:   HBG459705
- OMA:   GGRKTYG
- ProtClustDB:   CLSK880407
- BioCyc:   EcoCyc:HYCELARGE-MONOMER
- BioCyc:   MetaCyc:HYCELARGE-MONOMER
- Genevestigator:   P16431
- InterPro:   IPR001135
- InterPro:   IPR001268
- InterPro:   IPR014029
- InterPro:   IPR001501
- ProDom:   PD001581

Pfam domain/function: PF00329 Complex1_30kDa; PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases

EC number: NA

Molecular weight: Translated: 64981; Mature: 64850

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS00535 COMPLEX1_49K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPEVVEFLYYKQGGWLSVL
CCHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEEEEEECCHHHHHHHHHCCCCCEEEEE
FGNDERKLNGHYAVYYVLSMEKGTKCWITVRVEVDANKPEYPSVTPRVPAAVWGEREVRD
ECCCCCEECCCEEEEEEEEECCCCEEEEEEEEEECCCCCCCCCCCCCCCHHHCCCHHHHH
MYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPAPTTDAETYEFINELGDKKNN
HHCCCCCCCCCCCEEECCCCCCHHCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHCCCCCC
VVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEKLAETRMGYNEVTFLSDRV
EEECCCEEECCCCCCEEEEEECCCCEECCCEEEEEEHHHHHHHHHHHCCCCCEEEHHHHH
CGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVERLHSHLLNLGLACHFTGFDSG
HHHHCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHH
FMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRRDLLKDDMIQTRQLAQQMRREVQELV
HHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DVLLSTPNMEQRTVGIGRLDPEIARDFSNVGPMVRASGHARDTRADHPFVGYGLLPMEVH
HHHHCCCCCHHHCCCCCCCCHHHHHHHHHCCCCEECCCCCCCCCCCCCEEEECEEEEEEC
SEQGCDVISRLKVRINEVYTALNMIDYGLDNLPGGPLMVEGFTYIPHRFALGFAEAPRGD
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEECCHHHHCCCCCCCCCC
DIHWSMTGDNQKLYRWRCRAATYANWPTLRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTV
CEEEEEECCCCEEEEEEECCCCCCCCCEEEEEEECCCCCCCCEEEECCCCHHCCCCCEEE
VDVRKKKSKVVPYKELERYSIERKNSPLK
EEEHHHHCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPEVVEFLYYKQGGWLSVL
CHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEEEEEECCHHHHHHHHHCCCCCEEEEE
FGNDERKLNGHYAVYYVLSMEKGTKCWITVRVEVDANKPEYPSVTPRVPAAVWGEREVRD
ECCCCCEECCCEEEEEEEEECCCCEEEEEEEEEECCCCCCCCCCCCCCCHHHCCCHHHHH
MYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPAPTTDAETYEFINELGDKKNN
HHCCCCCCCCCCCEEECCCCCCHHCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHCCCCCC
VVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEKLAETRMGYNEVTFLSDRV
EEECCCEEECCCCCCEEEEEECCCCEECCCEEEEEEHHHHHHHHHHHCCCCCEEEHHHHH
CGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVERLHSHLLNLGLACHFTGFDSG
HHHHCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHH
FMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRRDLLKDDMIQTRQLAQQMRREVQELV
HHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DVLLSTPNMEQRTVGIGRLDPEIARDFSNVGPMVRASGHARDTRADHPFVGYGLLPMEVH
HHHHCCCCCHHHCCCCCCCCHHHHHHHHHCCCCEECCCCCCCCCCCCCEEEECEEEEEEC
SEQGCDVISRLKVRINEVYTALNMIDYGLDNLPGGPLMVEGFTYIPHRFALGFAEAPRGD
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEECCHHHHCCCCCCCCCC
DIHWSMTGDNQKLYRWRCRAATYANWPTLRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTV
CEEEEEECCCCEEEEEEECCCCCCCCCEEEEEEECCCCCCCCEEEECCCCHHCCCCCEEE
VDVRKKKSKVVPYKELERYSIERKNSPLK
EEEHHHHCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503; 8125094