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The map label for this gene is hycD

Identifier: 157162168

GI number: 157162168

Start: 2863907

End: 2864830

Strand: Reverse

Name: hycD

Synonym: EcHS_A2858

Alternate gene names: 157162168

Gene position: 2864830-2863907 (Counterclockwise)

Preceding gene: 157162169

Following gene: 157162167

Centisome position: 61.69

GC content: 58.44

Gene sequence:

>924_bases
ATGAGTGTTTTATATCCGTTAATTCAGGCGCTGGTGTTATTTGCCGTTGCGCCGCTGCTCTCCGGTATAACCCGCGTGGC
GCGCGCCCGCTTGCATAACCGTCGCGGGCCGGGCGTGTTGCAGGAGTATCGCGACATTATCAAACTGCTGGGGCGTCAGA
GCGTCGGCCCGGATGCCTCCGGCTGGGTGTTCCGCCTGACGCCGTATGTGATGGTGGGCGTCATGCTGACTATCGCTACT
GCGCTGCCGGTGGTGACCGTCGGTTCTCCGCTGCCGCAACTGGGTGATTTGATCACCTTACTGTATCTCTTTGCCATCGC
GCGTTTCTTCTTTGCCATTTCTGGTCTGGATACCGGTAGCCCGTTTACCGCTATCGGCGCGAGCCGTGAAGCGATGCTTG
GCGTGCTGGTCGAACCGATGCTGCTGCTTGGTCTGTGGGTTGCCGCACAGGTTGCCGGTTCCACCAACATCAGCAACATC
ACCGACACCGTTTATCACTGGCCGCTGAGCCAGAGCATCCCGCTGGTACTGGCGCTTTGTGCCTGTGCGTTCGCCACCTT
TATCGAAATGGGCAAACTGCCGTTCGACCTGGCGGAAGCCGAGCAGGAGCTGCAGGAAGGCCCGCTCTCTGAATACAGCG
GCAGCGGCTTTGGCGTCATGAAATGGGGTATCAGCCTGAAACAGCTGGTGGTGTTGCAGATGTTCGTCGGGGTGTTTATT
CCGTGGGGACAAATGGAAACCTTCACCGCCGGTGGACTGCTGCTGGCGCTGGTGATTGCCATCGTAAAACTGGTGGTCGG
CGTCCTGGTTATCGCGCTGTTCGAAAACAGCATGGCCCGTCTGCGTCTTGATATTACTCCGCGCATTACCTGGGCTGGGT
TTGGCTTTGCATTTTTAGCGTTCGTCTCCTTGCTGGCGGCGTGA

Upstream 100 bases:

>100_bases
CGGGCTGGCAGTGCGAGGGGAGTGCGTTGCTGTTCCGCCGGATGGCGCTGGTTGAACTGGCGGTACTGGTGGTGATTATT
GTTTCACGAGGAGCCTGAGA

Downstream 100 bases:

>100_bases
TTAAAGAGAGTTTGAGCATGTCTGAAGAAAAATTAGGTCAACATTATCTCGCCGCGCTGAATGAGGCATTTCCGGGCGTC
GTGCTGGACCACGCCTGGCA

Product: formate hydrogenlyase, subunit D

Products: NA

Alternate protein names: FHL subunit 4; Hydrogenase 3 component D

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYRDIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIAT
ALPVVTVGSPLPQLGDLITLLYLFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFGVMKWGISLKQLVVLQMFVGVFI
PWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFENSMARLRLDITPRITWAGFGFAFLAFVSLLAA

Sequences:

>Translated_307_residues
MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYRDIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIAT
ALPVVTVGSPLPQLGDLITLLYLFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFGVMKWGISLKQLVVLQMFVGVFI
PWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFENSMARLRLDITPRITWAGFGFAFLAFVSLLAA
>Mature_306_residues
SVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYRDIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATA
LPVVTVGSPLPQLGDLITLLYLFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNIT
DTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFGVMKWGISLKQLVVLQMFVGVFIP
WGQMETFTAGGLLLALVIAIVKLVVGVLVIALFENSMARLRLDITPRITWAGFGFAFLAFVSLLAA

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG0650

COG function: function code C; Formate hydrogenlyase subunit 4

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 1 family

Homologues:

Organism=Escherichia coli, GI1789077, Length=307, Percent_Identity=100, Blast_Score=605, Evalue=1e-174,
Organism=Escherichia coli, GI87082115, Length=303, Percent_Identity=52.1452145214521, Blast_Score=285, Evalue=4e-78,
Organism=Escherichia coli, GI1788618, Length=306, Percent_Identity=25.8169934640523, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HYCD_ECOLI (P16430)

Other databases:

- EMBL:   X17506
- EMBL:   U29579
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   S08622
- RefSeq:   AP_003289.1
- RefSeq:   NP_417202.1
- ProteinModelPortal:   P16430
- STRING:   P16430
- EnsemblBacteria:   EBESCT00000000443
- EnsemblBacteria:   EBESCT00000014953
- GeneID:   948994
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW2692
- KEGG:   eco:b2722
- EchoBASE:   EB0472
- EcoGene:   EG10477
- eggNOG:   COG0650
- GeneTree:   EBGT00050000009143
- HOGENOM:   HBG727670
- OMA:   FFPWGQM
- ProtClustDB:   CLSK880406
- BioCyc:   EcoCyc:HYCD-MONOMER
- BioCyc:   MetaCyc:HYCD-MONOMER
- Genevestigator:   P16430
- InterPro:   IPR001694
- InterPro:   IPR018086
- PANTHER:   PTHR11432

Pfam domain/function: PF00146 NADHdh

EC number: NA

Molecular weight: Translated: 33030; Mature: 32899

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: PS00667 COMPLEX1_ND1_1; PS00668 COMPLEX1_ND1_2

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x16851c6c)-; HASH(0x16249cc0)-; HASH(0x16d4d704)-; HASH(0x14ac1114)-; HASH(0x168ead34)-; HASH(0x16b8bf28)-; HASH(0x15694f40)-; HASH(0x1660e818)-;

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYRDIIKLLGRQSVGPDAS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCC
GWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLYLFAIARFFFAISGLDTGS
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNITDTVYHWPLSQSIPLVLALC
CCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCHHHHHHHH
ACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFGVMKWGISLKQLVVLQMFVGVFI
HHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
PWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFENSMARLRLDITPRITWAGFGFAFLA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEECCCCEEHHHHHHHHHH
FVSLLAA
HHHHHCH
>Mature Secondary Structure 
SVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYRDIIKLLGRQSVGPDAS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCC
GWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLYLFAIARFFFAISGLDTGS
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNITDTVYHWPLSQSIPLVLALC
CCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCHHHHHHHH
ACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFGVMKWGISLKQLVVLQMFVGVFI
HHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
PWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFENSMARLRLDITPRITWAGFGFAFLA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEECCCCEEHHHHHHHHHH
FVSLLAA
HHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503