Click here to switch to the map view.

The map label for this gene is ascB

Identifier: 157162162

GI number: 157162162

Start: 2858408

End: 2859832

Strand: Direct

Name: ascB

Synonym: EcHS_A2852

Alternate gene names: 157162162

Gene position: 2858408-2859832 (Clockwise)

Preceding gene: 157162161

Following gene: 157162173

Centisome position: 61.56

GC content: 51.51

Gene sequence:

>1425_bases
ATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCAGTCTGAAGGTGCGTTCCGTGAAGGTGA
CAAAGGTCTGACCACTGTCGATATGATCCCACACGGCGAGCATCGAATGGCGGTGAAACTGGGGCTGGAAAAACGTTTTC
AGTTGCGAGATGACGAGTTTTATCCCAGCCATGAGGCGACGGATTTTTATCATCGTTATAAAGAAGATATCGCCCTGATG
GCAGAGATGGGATTCAAGGTTTTCCGTACCTCAATTGCCTGGAGCCGTCTCTTTCCGCAGGGCGATGAAATCACGCCCAA
TCAGCAGGGCATTGCTTTTTATCGTTCTGTCTTTGAAGAGTGTAAAAAGTACGGTATCGAACCGCTGGTCACGTTGTGCC
ACTTCGATGTGCCGATGCATCTGGTCACCGAATATGGCTCCTGGCGTAACCGCAAGCTGGTGGAGTTTTTCAGCCGCTAC
GCCAGAACCTGCTTTGAAGCATTTGATGGTCTGGTGAAATACTGGCTAACCTTCAATGAAATCAACATTATGTTGCATAG
CCCGTTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGC
TGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGT
AACTTCTACCCTTACAGTTGCAAGCCGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGA
TGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGG
GCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATG
AACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGG
AATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACG
GCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCGCATCAGCTACTTACGCGAACATATC
CGCGCAATGGGCGAAGCGATTGCAGACGGCATTCCGCTGATGGGCTACACCACATGGGGCTGTATTGATTTAGTTTCCGC
CTCTACGGGTGAAATGAGCAAACGCTACGGCTTTGTCTTTGTTGACCGTGACGACGCAGGCAACGGTACGCTGACGCGCA
CGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAG

Upstream 100 bases:

>100_bases
TTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTA
AGTCTTAATTGAGGATGAAA

Downstream 100 bases:

>100_bases
GTAACAGTGCCGGATGCGGCGTGAACGCCTGATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGC
ATCGGGCAATTTAGCATTTG

Product: cryptic 6-phospho-beta-glucosidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MSVFPESFLWGGALAANQSEGAFREGDKGLTTVDMIPHGEHRMAVKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALM
AEMGFKVFRTSIAWSRLFPQGDEITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASALATKIAHEVNPQNQVGCMLAGG
NFYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPAYSARVFREKGVTINKAPGDDEILKNTVDFVSFSYYASRCASAEM
NANNSSAANVVKSLRNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDEFAANGEINDDYRISYLREHI
RAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVFVDRDDAGNGTLTRTRKKSFWWYKKVIASNGEDLE

Sequences:

>Translated_474_residues
MSVFPESFLWGGALAANQSEGAFREGDKGLTTVDMIPHGEHRMAVKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALM
AEMGFKVFRTSIAWSRLFPQGDEITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASALATKIAHEVNPQNQVGCMLAGG
NFYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPAYSARVFREKGVTINKAPGDDEILKNTVDFVSFSYYASRCASAEM
NANNSSAANVVKSLRNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDEFAANGEINDDYRISYLREHI
RAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVFVDRDDAGNGTLTRTRKKSFWWYKKVIASNGEDLE
>Mature_473_residues
SVFPESFLWGGALAANQSEGAFREGDKGLTTVDMIPHGEHRMAVKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMA
EMGFKVFRTSIAWSRLFPQGDEITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRYA
RTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASALATKIAHEVNPQNQVGCMLAGGN
FYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPAYSARVFREKGVTINKAPGDDEILKNTVDFVSFSYYASRCASAEMN
ANNSSAANVVKSLRNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDEFAANGEINDDYRISYLREHIR
AMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVFVDRDDAGNGTLTRTRKKSFWWYKKVIASNGEDLE

Specific function: Can hydrolyze salicin, cellobiose, and probably arbutin

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family

Homologues:

Organism=Homo sapiens, GI110681710, Length=499, Percent_Identity=29.8597194388778, Blast_Score=178, Evalue=8e-45,
Organism=Homo sapiens, GI32481206, Length=494, Percent_Identity=28.5425101214575, Blast_Score=165, Evalue=8e-41,
Organism=Homo sapiens, GI13273313, Length=487, Percent_Identity=27.3100616016427, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI24497614, Length=490, Percent_Identity=26.3265306122449, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI28376633, Length=491, Percent_Identity=23.8289205702648, Blast_Score=124, Evalue=2e-28,
Organism=Escherichia coli, GI1789070, Length=474, Percent_Identity=100, Blast_Score=996, Evalue=0.0,
Organism=Escherichia coli, GI2367270, Length=474, Percent_Identity=54.2194092827004, Blast_Score=541, Evalue=1e-155,
Organism=Escherichia coli, GI2367174, Length=478, Percent_Identity=51.255230125523, Blast_Score=503, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI17552856, Length=487, Percent_Identity=29.9794661190965, Blast_Score=179, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17539390, Length=495, Percent_Identity=30.3030303030303, Blast_Score=169, Evalue=4e-42,
Organism=Drosophila melanogaster, GI21356577, Length=503, Percent_Identity=25.0497017892644, Blast_Score=125, Evalue=6e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ASCB_ECOLI (P24240)

Other databases:

- EMBL:   M73326
- EMBL:   U29579
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   H65051
- RefSeq:   AP_003283.1
- RefSeq:   NP_417196.1
- ProteinModelPortal:   P24240
- SMR:   P24240
- STRING:   P24240
- EnsemblBacteria:   EBESCT00000000157
- EnsemblBacteria:   EBESCT00000014881
- GeneID:   947460
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW2686
- KEGG:   eco:b2716
- EchoBASE:   EB0083
- EcoGene:   EG10085
- eggNOG:   COG2723
- GeneTree:   EBGT00050000010166
- HOGENOM:   HBG316462
- OMA:   SWRNRKM
- ProtClustDB:   PRK09852
- BioCyc:   EcoCyc:EG10085-MONOMER
- BioCyc:   MetaCyc:EG10085-MONOMER
- Genevestigator:   P24240
- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781
- Gene3D:   G3DSA:3.20.20.80
- PANTHER:   PTHR10353
- PRINTS:   PR00131

Pfam domain/function: PF00232 Glyco_hydro_1; SSF51445 Glyco_hydro_cat

EC number: =3.2.1.86

Molecular weight: Translated: 53935; Mature: 53804

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1; PS00653 GLYCOSYL_HYDROL_F1_2

Important sites: ACT_SITE 180-180 ACT_SITE 372-372

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVFPESFLWGGALAANQSEGAFREGDKGLTTVDMIPHGEHRMAVKLGLEKRFQLRDDEF
CCCCCCHHHCCCEEECCCCCCCCCCCCCCCEEEEECCCCCCEEEEEECCHHHCCCCCCCC
YPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGDEITPNQQGIAFYRSVFEE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
CKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRYARTCFEAFDGLVKYWLTFNE
HHHCCCCHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEE
INIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASALATKIAHEVNPQNQVGCMLAGG
EEEEEECCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECC
NFYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPAYSARVFREKGVTINKAPGDDEILK
CCCCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHCCCEEECCCCCHHHHH
NTVDFVSFSYYASRCASAEMNANNSSAANVVKSLRNPYLQVSDWGWGIDPLGLRITMNMM
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEHHHH
YDRYQKPLFLVENGLGAKDEFAANGEINDDYRISYLREHIRAMGEAIADGIPLMGYTTWG
HHHHCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
CIDLVSASTGEMSKRYGFVFVDRDDAGNGTLTRTRKKSFWWYKKVIASNGEDLE
HHHHHCCCCCCHHHHCCEEEEECCCCCCCCEEECHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SVFPESFLWGGALAANQSEGAFREGDKGLTTVDMIPHGEHRMAVKLGLEKRFQLRDDEF
CCCCCHHHCCCEEECCCCCCCCCCCCCCCEEEEECCCCCCEEEEEECCHHHCCCCCCCC
YPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGDEITPNQQGIAFYRSVFEE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
CKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRYARTCFEAFDGLVKYWLTFNE
HHHCCCCHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEE
INIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASALATKIAHEVNPQNQVGCMLAGG
EEEEEECCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECC
NFYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPAYSARVFREKGVTINKAPGDDEILK
CCCCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHCCCEEECCCCCHHHHH
NTVDFVSFSYYASRCASAEMNANNSSAANVVKSLRNPYLQVSDWGWGIDPLGLRITMNMM
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEHHHH
YDRYQKPLFLVENGLGAKDEFAANGEINDDYRISYLREHIRAMGEAIADGIPLMGYTTWG
HHHHCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
CIDLVSASTGEMSKRYGFVFVDRDDAGNGTLTRTRKKSFWWYKKVIASNGEDLE
HHHHHCCCCCCHHHHCCEEEEECCCCCCCCEEECHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1630307; 9278503