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The map label for this gene is ascF

Identifier: 157162161

GI number: 157162161

Start: 2856942

End: 2858399

Strand: Direct

Name: ascF

Synonym: EcHS_A2851

Alternate gene names: 157162161

Gene position: 2856942-2858399 (Clockwise)

Preceding gene: 157162157

Following gene: 157162162

Centisome position: 61.53

GC content: 56.17

Gene sequence:

>1458_bases
ATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGGCACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCA
CTGTATGACGCGGTTGCGCTTTGTTATCAAAGATGATGCGCTTATCGACAGCCCGACGTTAAAAACCATCCCCGGCGTGC
TCGGCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAGCCTTTCAGGAAGTCGTCAGC
CTGCTGCCGGGAGATATGCAGCCCGCACAGCCCGTGGGTAAACCCAAACTCACGCTACGTCGCATTGGTGCGGGGATCCT
CGATGCGCTGATCGGCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGTCAAACTGCTGGCAATGATCC
TCGAGATGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTC
CTGCCGCTGATGGTCGCGGCATCTGCCGCCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCT
GGTACATCCGAGTTTTATTGAACTGATGGCGAAAGCGGCCCAGGGTGAACATGTCGAATTTGCCCTGATTCCGGTCACCG
CGGTGAAATACACCTACACGGTGATCCCGGCGCTGGTGATGACCTGGTGCCTGTCATATATCGAACGCTGGGTGGACAGC
ATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGATTGCCGCACCGCTGGCAATCCTGCTGAT
TGGCCCGATTGGTATCTGGATCGGTAGCGCCATTTCGGCGCTGGTTTACACCATTCATGGTTATCTGGGCTGGCTTTCAG
TCGCCATTATGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCACCGCGTCTTTACGCCAACCATCATTCAGACC
ATTGCCGAAACCGGCAAAGAAGGGATGGTCATGCCGTCAGAGATCGGCGCTAACCTGTCGCTGGGCGGTTCATCACTGGC
AGTGGCGTGGAAAACGAAAAACCCGGAACTGCGTCAGACGGCGCTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATTT
CCGAACCGGCGTTATATGGCGTGGCGATCCGCCTGAAACGTCCGCTTATCGCCAGTCTTATCAGCGGTTTTATTTGCGGC
GCGGTTGCCGGTATGGCGGGGCTTGCCAGCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAGTTCTTCGATCC
GGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGC
TCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAA
GAAGTAAGTCTTAATTGA

Upstream 100 bases:

>100_bases
TGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTACCCTAC
TGATAACAGGATAAAATCCG

Downstream 100 bases:

>100_bases
GGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCAGTCTGAAGGTGCGTTCCGT
GAAGGTGACAAAGGTCTGAC

Product: cellobiose/arbutin/salicin-specific PTS system components IIBC

Products: NA

Alternate protein names: EIIBC-Asc; EII-Asc; Arbutin-, cellobiose-, and salicin-specific phosphotransferase enzyme IIB component; PTS system arbutin-, cellobiose-, and salicin-specific EIIB component; Arbutin, cellobiose, and salicin permease IIC component; PTS system arbutin-, cellobiose-, and salicin-specific EIIC component

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDALIDSPTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVS
LLPGDMQPAQPVGKPKLTLRRIGAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYTYTVIPALVMTWCLSYIERWVDS
ITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGSAISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQT
IAETGKEGMVMPSEIGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIASLISGFICG
AVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFILTLLLGFEDIPVEEAAAQARKYQSVQPTVAK
EVSLN

Sequences:

>Translated_485_residues
MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDALIDSPTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVS
LLPGDMQPAQPVGKPKLTLRRIGAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYTYTVIPALVMTWCLSYIERWVDS
ITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGSAISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQT
IAETGKEGMVMPSEIGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIASLISGFICG
AVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFILTLLLGFEDIPVEEAAAQARKYQSVQPTVAK
EVSLN
>Mature_484_residues
AKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDALIDSPTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSL
LPGDMQPAQPVGKPKLTLRRIGAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFFL
PLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYTYTVIPALVMTWCLSYIERWVDSI
TPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGSAISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTI
AETGKEGMVMPSEIGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIASLISGFICGA
VAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFILTLLLGFEDIPVEEAAAQARKYQSVQPTVAKE
VSLN

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain

Homologues:

Organism=Escherichia coli, GI48994906, Length=485, Percent_Identity=100, Blast_Score=966, Evalue=0.0,
Organism=Escherichia coli, GI1790159, Length=464, Percent_Identity=32.1120689655172, Blast_Score=208, Evalue=6e-55,
Organism=Escherichia coli, GI2367362, Length=418, Percent_Identity=27.511961722488, Blast_Score=138, Evalue=6e-34,
Organism=Escherichia coli, GI1788769, Length=487, Percent_Identity=25.4620123203285, Blast_Score=101, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTIBC_ECOLI (P24241)

Other databases:

- EMBL:   M73326
- EMBL:   U29579
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   G65051
- RefSeq:   AP_003282.1
- RefSeq:   YP_026182.1
- ProteinModelPortal:   P24241
- SMR:   P24241
- STRING:   P24241
- EnsemblBacteria:   EBESCT00000003392
- EnsemblBacteria:   EBESCT00000016996
- GeneID:   947154
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW5435
- KEGG:   eco:b2715
- EchoBASE:   EB0084
- EcoGene:   EG10086
- eggNOG:   COG1263
- GeneTree:   EBGT00050000009157
- HOGENOM:   HBG673521
- OMA:   PANPMTI
- ProtClustDB:   PRK09796
- BioCyc:   EcoCyc:ASCF-MONOMER
- Genevestigator:   P24241
- InterPro:   IPR018113
- InterPro:   IPR004719
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- Gene3D:   G3DSA:3.30.1360.60
- TIGRFAMs:   TIGR00826
- TIGRFAMs:   TIGR00852

Pfam domain/function: PF00367 PTS_EIIB; PF02378 PTS_EIIC; SSF55604 PTS_EIIB

EC number: =2.7.1.69

Molecular weight: Translated: 51027; Mature: 50895

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: PS51098 PTS_EIIB_TYPE_1; PS01035 PTS_EIIB_TYPE_1_CYS; PS51103 PTS_EIIC_TYPE_1

Important sites: ACT_SITE 28-28

Signals:

None

Transmembrane regions:

HASH(0x169173c0)-; HASH(0x16e8a330)-; HASH(0x16509458)-; HASH(0x16e07830)-; HASH(0x16bcab3c)-; HASH(0x16e65de4)-; HASH(0x165cd270)-; HASH(0x16d09458)-; HASH(0x16bfb480)-; HASH(0x163953ec)-;

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDALIDSPTLKTIPGVLGVVRSD
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCC
NQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRIGAGILDALIGTMSPLIPA
CCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFFLPLMVAASAAIKFKTNMSLA
HHCHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHCCCHHHHHHHHHHHHHHHEEECCCHHH
IAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYTYTVIPALVMTWCLSYIERWVDS
HHHHHHHHCHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGSAISALVYTIHGYLGWLSVAIMGAL
HCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSEIGANLSLGGSSLAVAWKTKNPELRQT
HHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHHCCCEEECCCEEEEEEECCCCHHHHH
ALAAAASAIMAGISEPALYGVAIRLKRPLIASLISGFICGAVAGMAGLASHSMAAPGLFT
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
SVQFFDPANPMSIVWVFAVMALAVVLSFILTLLLGFEDIPVEEAAAQARKYQSVQPTVAK
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHH
EVSLN
HCCCH
>Mature Secondary Structure 
AKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDALIDSPTLKTIPGVLGVVRSD
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCC
NQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRIGAGILDALIGTMSPLIPA
CCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFFLPLMVAASAAIKFKTNMSLA
HHCHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHCCCHHHHHHHHHHHHHHHEEECCCHHH
IAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYTYTVIPALVMTWCLSYIERWVDS
HHHHHHHHCHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGSAISALVYTIHGYLGWLSVAIMGAL
HCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSEIGANLSLGGSSLAVAWKTKNPELRQT
HHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHHCCCEEECCCEEEEEEECCCCHHHHH
ALAAAASAIMAGISEPALYGVAIRLKRPLIASLISGFICGAVAGMAGLASHSMAAPGLFT
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
SVQFFDPANPMSIVWVFAVMALAVVLSFILTLLLGFEDIPVEEAAAQARKYQSVQPTVAK
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHH
EVSLN
HCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1630307; 9278503