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The map label for this gene is hyfF [H]

Identifier: 157161946

GI number: 157161946

Start: 2628860

End: 2630440

Strand: Direct

Name: hyfF [H]

Synonym: EcHS_A2620

Alternate gene names: 157161946

Gene position: 2628860-2630440 (Clockwise)

Preceding gene: 157161945

Following gene: 157161947

Centisome position: 56.61

GC content: 53.95

Gene sequence:

>1581_bases
ATGAGTTATTCTGTGATGTTCGCTTTACTCCTGCTCACGCCGCTGCTTTTTTCGCTGCTCTGTTTTGCCTGCCGGAAACG
GAGACTTTCTGCGACTCGCACGGTGACCGTATTACATAGCTTAGGGATCACACTGCTGCTGATTCTGGCACTCTGGGTGG
TCCAAACTGCCGCTGATGCAGGAGAAATATTCGCTGCGGGACTGTGGCTTCATATTGATGGTCTGGGCGGTTTGTTCCTC
GCCATTCTTGGTGTGATTGGCTTTCTCACCGGTATTTACTCGATTGGCTACATGCGTCATGAAGTGGCACACGGCGAGCT
TTCACCCGTTACGCTGTGCGATTACTACGGTTTCTTCCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAGCAATA
ACCTGATTGTGATGTGGGCGGCGATCGAAGCCACCACCTTAAGCTCGGCGTTTCTGGTAGGCATTTACGGTCAGCGTTCA
TCGCTGGAAGCTGCATGGAAGTACATCATTATTTGTACTGTTGGTGTCGCTTTTGGTCTGTTCGGTACCGTGCTGGTATA
CGCCAACGCCGCCAGCGTTATGCCGCAGGCAGAAATGGCGATATTCTGGAGCGAGGTTCTTAAGCAATCGTCCTTGCTTG
ACCCAACATTAATGCTGTTGGCCTTTGTGTTTTTGCTAATTGGCTTTGGTACCAAAACCGGGCTATTTCCCATGCACGCC
TGGCTGCCGGATGCTCACAGTGAAGCGCCGAGTCCGGTCAGCGCCCTGCTCTCCGCCGTATTGCTGAACTGCGCGCTGTT
GGTGCTGATTCGCTATTACATCATTATTTGCCAAGCCATCGGCAGCGATTTCCCCAACCGGTTGTTGCTCATCTTCGGCA
TGTTGTCGGTTGCCGTGGCGGCATTTTTCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAG
AACATGGGGCTGGTCGCGGTGGCGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCA
CAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCT
GTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTC
AACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATT
GCTGTTAACGCTGGTGCTGGCGGGCCTGGTACGGATGGCTGCGCGGGTGTTAATGGCGAAACCGCCGCAGGCCGTTAACC
GGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCA
CAACCTGTCATCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCAC
CTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAA

Upstream 100 bases:

>100_bases
ATCTTCGCCGTCATTGTGATGGTGTTACTGGCAAGAAAAATATGGCGTACCCACGGCACGCTGGACGTGAACAACTTGAC
CGCGCTGAAGGGATAATGAG

Downstream 100 bases:

>100_bases
TTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGAC
AAACGCCTGAACAGGTCACC

Product: hydrogenase 4 subunit F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MSYSVMFALLLLTPLLFSLLCFACRKRRLSATRTVTVLHSLGITLLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFL
AILGVIGFLTGIYSIGYMRHEVAHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
SLEAAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLMLLAFVFLLIGFGTKTGLFPMHA
WLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIIICQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVE
NMGLVAVALGIGGPLGIFAALLHILNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGALALAGMPPF
NIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQAVNRGDLGWLTTSPMVILLVMMLAMGTHIP
QPVIRILAGASTIVLSGTHDLPAQRSTWHDFLPSGTASVSEKHSER

Sequences:

>Translated_526_residues
MSYSVMFALLLLTPLLFSLLCFACRKRRLSATRTVTVLHSLGITLLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFL
AILGVIGFLTGIYSIGYMRHEVAHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
SLEAAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLMLLAFVFLLIGFGTKTGLFPMHA
WLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIIICQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVE
NMGLVAVALGIGGPLGIFAALLHILNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGALALAGMPPF
NIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQAVNRGDLGWLTTSPMVILLVMMLAMGTHIP
QPVIRILAGASTIVLSGTHDLPAQRSTWHDFLPSGTASVSEKHSER
>Mature_525_residues
SYSVMFALLLLTPLLFSLLCFACRKRRLSATRTVTVLHSLGITLLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLA
ILGVIGFLTGIYSIGYMRHEVAHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRSS
LEAAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLMLLAFVFLLIGFGTKTGLFPMHAW
LPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIIICQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVEN
MGLVAVALGIGGPLGIFAALLHILNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGALALAGMPPFN
IFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQ
PVIRILAGASTIVLSGTHDLPAQRSTWHDFLPSGTASVSEKHSER

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=391, Percent_Identity=28.6445012787724, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI251831116, Length=185, Percent_Identity=31.3513513513514, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1788831, Length=526, Percent_Identity=99.8098859315589, Blast_Score=1039, Evalue=0.0,
Organism=Escherichia coli, GI1788827, Length=357, Percent_Identity=30.5322128851541, Blast_Score=148, Evalue=9e-37,
Organism=Escherichia coli, GI1788829, Length=433, Percent_Identity=30.0230946882217, Blast_Score=122, Evalue=6e-29,
Organism=Escherichia coli, GI1788613, Length=432, Percent_Identity=27.7777777777778, Blast_Score=119, Evalue=6e-28,
Organism=Escherichia coli, GI1788614, Length=439, Percent_Identity=28.0182232346241, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI2367154, Length=194, Percent_Identity=36.0824742268041, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI145693160, Length=371, Percent_Identity=26.4150943396226, Blast_Score=86, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 56710; Mature: 56579

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYSVMFALLLLTPLLFSLLCFACRKRRLSATRTVTVLHSLGITLLLILALWVVQTAADA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
GEIFAAGLWLHIDGLGGLFLAILGVIGFLTGIYSIGYMRHEVAHGELSPVTLCDYYGFFH
HHHHHHHHEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
LFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRSSLEAAWKYIIICTVGVAFGL
HHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHH
FGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLMLLAFVFLLIGFGTKTGLFPMHA
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCHHH
WLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIIICQAIGSDFPNRLLLIFGMLSVAVA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
AFFILVQRDIKRLLAYSSVENMGLVAVALGIGGPLGIFAALLHILNHSLAKTLLFCGSGN
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
VLLKYGTRDLNVVCGMLKIMPFTAVLFGGGALALAGMPPFNIFLSEFMTITAGLARNHLL
EEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHEECCCCHHHHHHHHHHHHHHHHHHHHHH
IIVLLLLLLTLVLAGLVRMAARVLMAKPPQAVNRGDLGWLTTSPMVILLVMMLAMGTHIP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
QPVIRILAGASTIVLSGTHDLPAQRSTWHDFLPSGTASVSEKHSER
HHHHHHHHCCCEEEEECCCCCCCCCCHHHHHCCCCCCHHHHHCCCC
>Mature Secondary Structure 
SYSVMFALLLLTPLLFSLLCFACRKRRLSATRTVTVLHSLGITLLLILALWVVQTAADA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
GEIFAAGLWLHIDGLGGLFLAILGVIGFLTGIYSIGYMRHEVAHGELSPVTLCDYYGFFH
HHHHHHHHEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
LFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRSSLEAAWKYIIICTVGVAFGL
HHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHH
FGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLMLLAFVFLLIGFGTKTGLFPMHA
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCHHH
WLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIIICQAIGSDFPNRLLLIFGMLSVAVA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
AFFILVQRDIKRLLAYSSVENMGLVAVALGIGGPLGIFAALLHILNHSLAKTLLFCGSGN
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
VLLKYGTRDLNVVCGMLKIMPFTAVLFGGGALALAGMPPFNIFLSEFMTITAGLARNHLL
EEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHEECCCCHHHHHHHHHHHHHHHHHHHHHH
IIVLLLLLLTLVLAGLVRMAARVLMAKPPQAVNRGDLGWLTTSPMVILLVMMLAMGTHIP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
QPVIRILAGASTIVLSGTHDLPAQRSTWHDFLPSGTASVSEKHSER
HHHHHHHHCCCEEEEECCCCCCCCCCHHHHHCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]