Click here to switch to the map view.

The map label for this gene is hyfG [H]

Identifier: 157161947

GI number: 157161947

Start: 2630430

End: 2632097

Strand: Direct

Name: hyfG [H]

Synonym: EcHS_A2621

Alternate gene names: 157161947

Gene position: 2630430-2632097 (Clockwise)

Preceding gene: 157161946

Following gene: 157161948

Centisome position: 56.65

GC content: 54.2

Gene sequence:

>1668_bases
GTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGA
TGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATT
ATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCC
CTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGTCAGT
CACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCGAGAAATTCGCGATATGTACGGGCTGATTCCGGTTGGCCTGCCGG
ATCAGCGTCGCCTGGTGTTGCCCGATGACTGGCCGGAAGATATGCATCCGCTGCGCAAAGATGCGATGGATTATCGACTG
CGCCCTGAACCGACGACTGATTCCGAAACGTATCCGTTTATCAATGAGGGCAACAGCGATGCGCGGGTGATCCCTGTCGG
CCCGCTGCATATCACCTCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGATGGCGAGCAAATTGTCGATGCTGATTACC
GCCTGTTTTATGTCCATCGCGGCATGGAGAAACTGGCAGAAACGCGGATGGGCTACAACGAAGTGACCTTCTTATCGGAC
CGCGTGTGTGGGATTTGCGGTTTTGCCCACAGTGTGGCCTATACCAATTCGGTTGAAAATGCACTGGGGATTGAGGTGCC
GCAACGAGCACATACTATTCGCTCGATTCTGCTGGAAGTCGAACGGCTACACAGTCATTTGCTTAACCTTGGCCTCTCCT
GCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTG
ATCGGGTCGCGTAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAAC
GCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCTGGTAGAGATGCTGCTTGCTACGCCGAATATGGAACAAC
GCACTCAGGGCATTGGCATTCTCGACCGACAAATCGCCCGTGATTTGCGCTTTGATCACCCCTACGCCGACTACGGCAAT
ATTCCAAAAACACTGTTTACCTTTACCGGCGGCGATGTTTTCTCCCGCGTGATGGTCCGTGTCAAAGAGACGTTTGATTC
GCTGGCAATGCTGGAATTTGCCCTCGACAACATGCAGGATACCCCACTGCTGACCGAAGGCTTTAGCTATAAACCTCACG
CATTCGCGCTGGGCTTTGTTGAAGCGCCACGCGGTGAAGACGTGCACTGGAGCATGCTCGGTGATAACCAAAAATTGTTC
CGCTGGCGCTGCCGTGCCGCCACCTACGCCAACTGGCCGGTATTGCGTTACATGCTGCGCGGCAATACCGTTTCTGACGC
ACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGT
CAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAA

Upstream 100 bases:

>100_bases
TCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGC
ATCTGTTTCGGAGAAACACA

Downstream 100 bases:

>100_bases
GGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGA
GGTCAGCCCAGGCTTTCGCG

Product: hydrogenase-4, G subunit

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 555; Mature: 555

Protein sequence:

>555_residues
MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINLLPDVVQYLYYQHDGWLPVLFGNDERTLNGHYAVYYA
LSMEGAEKCWIVVKALVDADSREFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
RPEPTTDSETYPFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYVHRGMEKLAETRMGYNEVTFLSD
RVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSILLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELL
IGSRKTYGLNLIGGVRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDLRFDHPYADYGN
IPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFALDNMQDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLF
RWRCRAATYANWPVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRNRSPLK

Sequences:

>Translated_555_residues
MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINLLPDVVQYLYYQHDGWLPVLFGNDERTLNGHYAVYYA
LSMEGAEKCWIVVKALVDADSREFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
RPEPTTDSETYPFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYVHRGMEKLAETRMGYNEVTFLSD
RVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSILLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELL
IGSRKTYGLNLIGGVRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDLRFDHPYADYGN
IPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFALDNMQDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLF
RWRCRAATYANWPVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRNRSPLK
>Mature_555_residues
MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINLLPDVVQYLYYQHDGWLPVLFGNDERTLNGHYAVYYA
LSMEGAEKCWIVVKALVDADSREFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
RPEPTTDSETYPFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYVHRGMEKLAETRMGYNEVTFLSD
RVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSILLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELL
IGSRKTYGLNLIGGVRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDLRFDHPYADYGN
IPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFALDNMQDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLF
RWRCRAATYANWPVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRNRSPLK

Specific function: Unknown

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 49 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI4758786, Length=383, Percent_Identity=25.3263707571802, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI260898743, Length=373, Percent_Identity=25.201072386059, Blast_Score=88, Evalue=2e-17,
Organism=Escherichia coli, GI1788832, Length=555, Percent_Identity=99.8198198198198, Blast_Score=1149, Evalue=0.0,
Organism=Escherichia coli, GI1789076, Length=568, Percent_Identity=70.2464788732394, Blast_Score=855, Evalue=0.0,
Organism=Escherichia coli, GI145693162, Length=493, Percent_Identity=28.1947261663286, Blast_Score=145, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI17555284, Length=392, Percent_Identity=24.4897959183673, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17568379, Length=392, Percent_Identity=24.234693877551, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI221459469, Length=386, Percent_Identity=24.8704663212435, Blast_Score=117, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24638644, Length=381, Percent_Identity=25.7217847769029, Blast_Score=113, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR001268
- InterPro:   IPR014029
- InterPro:   IPR001501
- ProDom:   PD001581 [H]

Pfam domain/function: PF00329 Complex1_30kDa; PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 63415; Mature: 63415

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00535 COMPLEX1_49K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINLLPDVVQYLYYQHDGWL
CCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHCCCCE
PVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSREFPSVTPRVPAAVWGERE
EEEECCCCCEECCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCHH
IRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRLRPEPTTDSETYPFINEGNSD
HHHHHCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCCC
ARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYVHRGMEKLAETRMGYNEVTFLSD
CEEEEECCEEECCCCCCEEEEEECCCCEECCCEEEEEEHHHHHHHHHHHCCCCCEEEHHH
RVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSILLEVERLHSHLLNLGLSCHFVGFD
HHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECC
TGFMQFFRVREKSMTMAELLIGSRKTYGLNLIGGVRRDILKEQRLQTLKLVREMRADVSE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVEMLLATPNMEQRTQGIGILDRQIARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVR
HHHHHHCCCCHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCHHHHHHHHHH
VKETFDSLAMLEFALDNMQDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLF
HHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCEEEEEEEECCCCCCEEEEEECCCHHHH
RWRCRAATYANWPVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYK
HEEHHHCCCCCCHHHHHHHCCCCCCCCCEEEECCCCHHCCCCCEEEEEEHHHHCCCCCHH
EIERYGIDRNRSPLK
HHHHHCCCCCCCCCC
>Mature Secondary Structure
MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINLLPDVVQYLYYQHDGWL
CCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHCCCCE
PVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSREFPSVTPRVPAAVWGERE
EEEECCCCCEECCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCHH
IRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRLRPEPTTDSETYPFINEGNSD
HHHHHCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCCC
ARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYVHRGMEKLAETRMGYNEVTFLSD
CEEEEECCEEECCCCCCEEEEEECCCCEECCCEEEEEEHHHHHHHHHHHCCCCCEEEHHH
RVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSILLEVERLHSHLLNLGLSCHFVGFD
HHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECC
TGFMQFFRVREKSMTMAELLIGSRKTYGLNLIGGVRRDILKEQRLQTLKLVREMRADVSE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVEMLLATPNMEQRTQGIGILDRQIARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVR
HHHHHHCCCCHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCHHHHHHHHHH
VKETFDSLAMLEFALDNMQDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLF
HHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCEEEEEEEECCCCCCEEEEEECCCHHHH
RWRCRAATYANWPVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYK
HEEHHHCCCCCCHHHHHHHCCCCCCCCCEEEECCCCHHCCCCCEEEEEEHHHHCCCCCHH
EIERYGIDRNRSPLK
HHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 4Fe-4S Cluster. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]