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The map label for this gene is amiA [H]

Identifier: 157161902

GI number: 157161902

Start: 2581049

End: 2581918

Strand: Direct

Name: amiA [H]

Synonym: EcHS_A2572

Alternate gene names: 157161902

Gene position: 2581049-2581918 (Clockwise)

Preceding gene: 157161897

Following gene: 157161903

Centisome position: 55.58

GC content: 50.11

Gene sequence:

>870_bases
ATGAGCACTTTTAAATCACTAAAAACACTCACTTCGCGCCGCCAGGTGCTGAAAGCCGGTTTGGCTGCCCTGACGTTGTC
AGGAATGTCGCAAGCCATCGCCAAAGACGAACCTTTAAAAACCAGCAACGGACACAGCAAGCCAAAAGCCAAAAAATCTG
GCGGCAAACGTGTCGTTGTTCTCGATCCAGGCCACGGCGGAATTGATACCGGAGCGATCGGACGCAACGGTTCGAAAGAA
AAACATGTGGTGCTGGCGATTGCTAAAAACGTCCGTTCCATTTTGCGTAATCATGGGATTGATGCGCGTTTAACGCGTTC
TGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATG
CCGATGGCTTTACCAACCCGAAAGCTTCCGGTGCTTCGGTATTTGCCCTCTCTAACCGTGGGGCAAGTAGCGCAATGGCG
AAATACCTGTCTGAACGCGAAAACCGCGCCGATGAAGTTGCCGGTAAAAAGGCGACTGACAAGGATCACCTATTGCAGCA
AGTGCTGTTTGATCTGGTGCAAACCGATACTATTAAAAACAGCCTGACGCTCGGCTCGCATATTCTGAAAAAGATTAAGC
CGGTGCATAAACTGCATAGCCGCAACACCGAACAAGCCGCGTTTGTGGTGTTGAAATCGCCGTCGGTTCCTTCGGTGCTG
GTGGAAACCTCGTTTATCACCAACCCGGAAGAAGAGCGGCTGTTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGC
GATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAA

Upstream 100 bases:

>100_bases
ATTGAAATGAAAAGTAAAACAATTCTCAACATCAAACCGTCGTAACGGATTACGCGATACGATATAACATCTGGAACTTT
ATTATTACAACTCAGGCCGT

Downstream 100 bases:

>100_bases
GTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGT
CGATGGCGCAGAATTTGTCG

Product: N-acetylmuramoyl-l-alanine amidase I

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MSTFKSLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNGHSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKE
KHVVLAIAKNVRSILRNHGIDARLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKASGASVFALSNRGASSAMA
KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKLHSRNTEQAAFVVLKSPSVPSVL
VETSFITNPEEERLLGTAAFRQKIATAIAEGVISYFHWFDNQKAHSKKR

Sequences:

>Translated_289_residues
MSTFKSLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNGHSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKE
KHVVLAIAKNVRSILRNHGIDARLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKASGASVFALSNRGASSAMA
KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKLHSRNTEQAAFVVLKSPSVPSVL
VETSFITNPEEERLLGTAAFRQKIATAIAEGVISYFHWFDNQKAHSKKR
>Mature_288_residues
STFKSLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNGHSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEK
HVVLAIAKNVRSILRNHGIDARLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKASGASVFALSNRGASSAMAK
YLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKLHSRNTEQAAFVVLKSPSVPSVLV
ETSFITNPEEERLLGTAAFRQKIATAIAEGVISYFHWFDNQKAHSKKR

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division. Can also act as powerful autolysin in the presence of murein synthesis inhibitors [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm. Note=Distributed throughout the periplasm in all cells [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=289, Percent_Identity=98.961937716263, Blast_Score=579, Evalue=1e-167,
Organism=Escherichia coli, GI87082163, Length=236, Percent_Identity=47.8813559322034, Blast_Score=223, Evalue=9e-60,
Organism=Escherichia coli, GI1790611, Length=244, Percent_Identity=38.1147540983607, Blast_Score=173, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 31402; Mature: 31271

Theoretical pI: Translated: 10.84; Mature: 10.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTFKSLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNGHSKPKAKKSGGKRVVV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCEEEE
LDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDARLTRSGDTFIPLYDRVEI
ECCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCEEEECCCCCEEEHHHHHHH
AHKHGADLFMSIHADGFTNPKASGASVFALSNRGASSAMAKYLSERENRADEVAGKKATD
HHHCCCEEEEEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
KDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKLHSRNTEQAAFVVLKSPSVPSVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEE
VETSFITNPEEERLLGTAAFRQKIATAIAEGVISYFHWFDNQKAHSKKR
EEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCC
>Mature Secondary Structure 
STFKSLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNGHSKPKAKKSGGKRVVV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCEEEE
LDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDARLTRSGDTFIPLYDRVEI
ECCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCEEEECCCCCEEEHHHHHHH
AHKHGADLFMSIHADGFTNPKASGASVFALSNRGASSAMAKYLSERENRADEVAGKKATD
HHHCCCEEEEEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
KDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKLHSRNTEQAAFVVLKSPSVPSVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEE
VETSFITNPEEERLLGTAAFRQKIATAIAEGVISYFHWFDNQKAHSKKR
EEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8300522; 9205837; 9278503; 1903834 [H]