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The map label for this gene is hemF [H]

Identifier: 157161903

GI number: 157161903

Start: 2581922

End: 2582821

Strand: Direct

Name: hemF [H]

Synonym: EcHS_A2573

Alternate gene names: 157161903

Gene position: 2581922-2582821 (Clockwise)

Preceding gene: 157161902

Following gene: 157161923

Centisome position: 55.6

GC content: 54.67

Gene sequence:

>900_bases
ATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGTCGA
TGGCGCAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTG
TTTTCGAACAGGCAGGCGTCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAA
CTTGCCGGGCGCAGTTTCGAGGCGATGGGCGTTTCACTGGTAGTGCATCCGCATAACCCGTATGTTCCCACCAGCCACGC
GAATGTGCGGTTTTTTATTGCCGAAAAACCGGGTGCCGATCCCGTCTGGTGGTTTGGCGGTGGCTTCGACTTAACCCCAT
TCTATGGTTTTGAAGAAGATGCTATTCACTGGCATCGCACCGCCCGTGACCTGTGCCTGCCATTTGGCGAAGACGTTTAT
CCCCGTTACAAAAAGTGGTGCGACGAATACTTCTACCTCAAACATCGCAACGAACAGCGCGGTATTGGCGGGCTGTTCTT
TGATGACCTGAACACGCCAGATTTCGACCGCTGTTTTGCCTTTATGCAGGCGGTAGGCAAAGGCTACACCGACGCTTATT
TACCAATTGTCGAGCGACGGAAAGCGATGGCCTACGGCGAGCGCGAGCGCAATTTCCAGTTATATCGTCGCGGTCGTTAT
GTCGAGTTCAATCTGGTCTGGGATCGCGGCACGCTGTTTGGCCTGCAAACTGGCGGGCGCACCGAGTCTATCCTGATGTC
AATGCCGCCACTGGTACGCTGGGAATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTATTA
AGGTCAGGGATTGGGTGTAA

Upstream 100 bases:

>100_bases
GGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCAC
ATTCGAAAAAGCGATAAGTT

Downstream 100 bases:

>100_bases
CTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGC
AAATTGCCCCAGATGCCAGA

Product: coproporphyrinogen III oxidase

Products: NA

Alternate protein names: Coprogen oxidase; Coproporphyrinogenase [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGGRSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPE
LAGRSFEAMGVSLVVHPHNPYVPTSHANVRFFIAEKPGADPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
PRYKKWCDEYFYLKHRNEQRGIGGLFFDDLNTPDFDRCFAFMQAVGKGYTDAYLPIVERRKAMAYGERERNFQLYRRGRY
VEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEYDYQPKDGSPEAALSEFIKVRDWV

Sequences:

>Translated_299_residues
MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGGRSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPE
LAGRSFEAMGVSLVVHPHNPYVPTSHANVRFFIAEKPGADPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
PRYKKWCDEYFYLKHRNEQRGIGGLFFDDLNTPDFDRCFAFMQAVGKGYTDAYLPIVERRKAMAYGERERNFQLYRRGRY
VEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEYDYQPKDGSPEAALSEFIKVRDWV
>Mature_299_residues
MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGGRSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPE
LAGRSFEAMGVSLVVHPHNPYVPTSHANVRFFIAEKPGADPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
PRYKKWCDEYFYLKHRNEQRGIGGLFFDDLNTPDFDRCFAFMQAVGKGYTDAYLPIVERRKAMAYGERERNFQLYRRGRY
VEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEYDYQPKDGSPEAALSEFIKVRDWV

Specific function: Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III [H]

COG id: COG0408

COG function: function code H; Coproporphyrinogen III oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aerobic coproporphyrinogen-III oxidase family [H]

Homologues:

Organism=Homo sapiens, GI41393599, Length=303, Percent_Identity=46.8646864686469, Blast_Score=284, Evalue=8e-77,
Organism=Escherichia coli, GI1788777, Length=299, Percent_Identity=100, Blast_Score=624, Evalue=1e-180,
Organism=Saccharomyces cerevisiae, GI6320249, Length=317, Percent_Identity=43.8485804416404, Blast_Score=258, Evalue=7e-70,
Organism=Drosophila melanogaster, GI17864388, Length=304, Percent_Identity=47.6973684210526, Blast_Score=286, Evalue=2e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001260
- InterPro:   IPR018375 [H]

Pfam domain/function: PF01218 Coprogen_oxidas [H]

EC number: =1.3.3.3 [H]

Molecular weight: Translated: 34323; Mature: 34323

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS01021 COPROGEN_OXIDASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGGRSRVLRNGGVFEQAGV
CCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCHHCCC
NFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYVPTSHANVRFFIAEKPGAD
CEEEECCCCCCCCCCCCCCHHCCCCEEECCEEEEEECCCCCCCCCCCCEEEEEEECCCCC
PVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVYPRYKKWCDEYFYLKHRNEQR
CEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCC
GIGGLFFDDLNTPDFDRCFAFMQAVGKGYTDAYLPIVERRKAMAYGERERNFQLYRRGRY
CCCCEEECCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHCCCE
VEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEYDYQPKDGSPEAALSEFIKVRDWV
EEEEEEECCCEEEEECCCCCCHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGGRSRVLRNGGVFEQAGV
CCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCHHCCC
NFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYVPTSHANVRFFIAEKPGAD
CEEEECCCCCCCCCCCCCCHHCCCCEEECCEEEEEECCCCCCCCCCCCEEEEEEECCCCC
PVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVYPRYKKWCDEYFYLKHRNEQR
CEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCC
GIGGLFFDDLNTPDFDRCFAFMQAVGKGYTDAYLPIVERRKAMAYGERERNFQLYRRGRY
CCCCEEECCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHCCCE
VEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEYDYQPKDGSPEAALSEFIKVRDWV
EEEEEEECCCEEEEECCCCCCHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA