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The map label for this gene is rfbA2 [H]

Identifier: 157161535

GI number: 157161535

Start: 2159678

End: 2160547

Strand: Reverse

Name: rfbA2 [H]

Synonym: EcHS_A2181

Alternate gene names: 157161535

Gene position: 2160547-2159678 (Counterclockwise)

Preceding gene: 157161536

Following gene: 157161534

Centisome position: 46.53

GC content: 58.05

Gene sequence:

>870_bases
ATGAAAGGTATTGTTCTGGCCGGCGGCTCCGGCACGCGTTTATATCCGATCACCCAGGGCGTCTCCAAGCAGCTGCTGCC
GATCTATGACAAGCCGATGATTTTCTACCCGGTGTCCGTCCTGATGCTGGCGGGGATTCGCGACATCCTGATCATCTCCA
CCCCGGACGACATGCCTTCCTTCCAGCGCCTGCTGGGTGACGGGTCGCAGTTCGGGGTGAACTTCAGTTACGCCATCCAG
CCGTCGCCGGACGGCCTGGCGCAGGCCTTTATCATTGGCGAGAAGTTCATCGGCAACGATGCCTGCGCCCTGGTGCTGGG
AGATAATATCTACTTTGGCCAGAGCTTCGGTAAAAAGCTGGAAGCGGCAGCGGCGAAGACTTCCGGTGCCACGGTGTTTG
GTTATCAGGTGCTGGATCCGGAGCGCTTCGGGGTGGTGGAGTTCGATGAGAACTTTAAGGCACTGTCTATCGAGGAGAAA
CCGCTGAAGCCGAAATCAGACTGGGCGGTGACCGGGCTCTATTTCTACGACAATAACGTGGTGGAGATGGCGAAGGACGT
GAAGCCTTCTGAACGCGGCGAGCTGGAGATCACCACCCTGAACCAGATGTATCTGGAGCAAGGTGACCTGCATGTTGAAC
TGCTGGGGCGCGGTTTTGCTTGGCTGGATACGGGCACCCACGACAGCCTGGTGGACGCGTCGCAGTTCATTCACACCATT
GAGAAGCGCCAGGGGATGAAAGTGGCCTGCCTGGAAGAGATAGCCTACCGTAATCAGTGGCTGTCGGCGGAAGGGGTGGC
GGCTCAGGCGGAACGCCTGAAGAAAACCGAGTACGGTGCTTATCTGAAGCGCCTGCTGAACGAGCGTTAA

Upstream 100 bases:

>100_bases
TTAACAACCAGACCTGGTGGCAGCGCGTGCTGGATGGCTCCTATGCCGGTGAGCGTCTGGGCCTAAATAACTGATATCTG
ACTGATTAAGGAATAGATTT

Downstream 100 bases:

>100_bases
TGCCTTCTTCGTCAACGACAGGATACAGAGAATGAAGATTTTACTGATTGGCAAGAATGGCCAGGTGGGCTGGGAACTGC
AGCGTTCACTGTCCACCCTC

Product: glucose-1-phosphate thymidylyltransferase

Products: NA

Alternate protein names: dTDP-glucose pyrophosphorylase; dTDP-glucose synthase [H]

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MKGIVLAGGSGTRLYPITQGVSKQLLPIYDKPMIFYPVSVLMLAGIRDILIISTPDDMPSFQRLLGDGSQFGVNFSYAIQ
PSPDGLAQAFIIGEKFIGNDACALVLGDNIYFGQSFGKKLEAAAAKTSGATVFGYQVLDPERFGVVEFDENFKALSIEEK
PLKPKSDWAVTGLYFYDNNVVEMAKDVKPSERGELEITTLNQMYLEQGDLHVELLGRGFAWLDTGTHDSLVDASQFIHTI
EKRQGMKVACLEEIAYRNQWLSAEGVAAQAERLKKTEYGAYLKRLLNER

Sequences:

>Translated_289_residues
MKGIVLAGGSGTRLYPITQGVSKQLLPIYDKPMIFYPVSVLMLAGIRDILIISTPDDMPSFQRLLGDGSQFGVNFSYAIQ
PSPDGLAQAFIIGEKFIGNDACALVLGDNIYFGQSFGKKLEAAAAKTSGATVFGYQVLDPERFGVVEFDENFKALSIEEK
PLKPKSDWAVTGLYFYDNNVVEMAKDVKPSERGELEITTLNQMYLEQGDLHVELLGRGFAWLDTGTHDSLVDASQFIHTI
EKRQGMKVACLEEIAYRNQWLSAEGVAAQAERLKKTEYGAYLKRLLNER
>Mature_289_residues
MKGIVLAGGSGTRLYPITQGVSKQLLPIYDKPMIFYPVSVLMLAGIRDILIISTPDDMPSFQRLLGDGSQFGVNFSYAIQ
PSPDGLAQAFIIGEKFIGNDACALVLGDNIYFGQSFGKKLEAAAAKTSGATVFGYQVLDPERFGVVEFDENFKALSIEEK
PLKPKSDWAVTGLYFYDNNVVEMAKDVKPSERGELEITTLNQMYLEQGDLHVELLGRGFAWLDTGTHDSLVDASQFIHTI
EKRQGMKVACLEEIAYRNQWLSAEGVAAQAERLKKTEYGAYLKRLLNER

Specific function: Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis [H]

COG id: COG1209

COG function: function code M; dTDP-glucose pyrophosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate thymidylyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790224, Length=289, Percent_Identity=74.0484429065744, Blast_Score=450, Evalue=1e-128,
Organism=Escherichia coli, GI1788351, Length=287, Percent_Identity=68.6411149825784, Blast_Score=417, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI133931050, Length=226, Percent_Identity=25.6637168141593, Blast_Score=71, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6320148, Length=254, Percent_Identity=26.7716535433071, Blast_Score=80, Evalue=3e-16,
Organism=Drosophila melanogaster, GI21355443, Length=249, Percent_Identity=22.8915662650602, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24644084, Length=249, Percent_Identity=22.8915662650602, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005907
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.24 [H]

Molecular weight: Translated: 32011; Mature: 32011

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGIVLAGGSGTRLYPITQGVSKQLLPIYDKPMIFYPVSVLMLAGIRDILIISTPDDMPS
CCCEEEECCCCCEEEEECCCCCCCCCCCCCCCEEEHHHHHHHHCCCCEEEEEECCCCCHH
FQRLLGDGSQFGVNFSYAIQPSPDGLAQAFIIGEKFIGNDACALVLGDNIYFGQSFGKKL
HHHHHCCCCCCCEEEEEEECCCCCHHHHEEEECHHHCCCCEEEEEECCCEEECCHHHHHH
EAAAAKTSGATVFGYQVLDPERFGVVEFDENFKALSIEEKPLKPKSDWAVTGLYFYDNNV
HHHHHCCCCCEEEEEEEECCCCCCEEEECCCCEEEEECCCCCCCCCCEEEEEEEEECCCH
VEMAKDVKPSERGELEITTLNQMYLEQGDLHVELLGRGFAWLDTGTHDSLVDASQFIHTI
HHHHHCCCCCCCCCEEEEEHHHHHHHCCCEEEEEECCCEEEEECCCCCHHHHHHHHHHHH
EKRQGMKVACLEEIAYRNQWLSAEGVAAQAERLKKTEYGAYLKRLLNER
HHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKGIVLAGGSGTRLYPITQGVSKQLLPIYDKPMIFYPVSVLMLAGIRDILIISTPDDMPS
CCCEEEECCCCCEEEEECCCCCCCCCCCCCCCEEEHHHHHHHHCCCCEEEEEECCCCCHH
FQRLLGDGSQFGVNFSYAIQPSPDGLAQAFIIGEKFIGNDACALVLGDNIYFGQSFGKKL
HHHHHCCCCCCCEEEEEEECCCCCHHHHEEEECHHHCCCCEEEEEECCCEEECCHHHHHH
EAAAAKTSGATVFGYQVLDPERFGVVEFDENFKALSIEEKPLKPKSDWAVTGLYFYDNNV
HHHHHCCCCCEEEEEEEECCCCCCEEEECCCCEEEEECCCCCCCCCCEEEEEEEEECCCH
VEMAKDVKPSERGELEITTLNQMYLEQGDLHVELLGRGFAWLDTGTHDSLVDASQFIHTI
HHHHHCCCCCCCCCEEEEEHHHHHHHCCCEEEEEECCCEEEEECCCCCHHHHHHHHHHHH
EKRQGMKVACLEEIAYRNQWLSAEGVAAQAERLKKTEYGAYLKRLLNER
HHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7692217 [H]