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The map label for this gene is rfbD [H]

Identifier: 157161534

GI number: 157161534

Start: 2158756

End: 2159646

Strand: Reverse

Name: rfbD [H]

Synonym: EcHS_A2180

Alternate gene names: 157161534

Gene position: 2159646-2158756 (Counterclockwise)

Preceding gene: 157161535

Following gene: 157161533

Centisome position: 46.51

GC content: 61.84

Gene sequence:

>891_bases
ATGAAGATTTTACTGATTGGCAAGAATGGCCAGGTGGGCTGGGAACTGCAGCGTTCACTGTCCACCCTCGGCGACGTGGT
CGCGGTGGACTACTTTGACAAAGAGCTGTGCGGCGACCTGACTAATCTGGAAGGCATCGCGCAGACCGTGCGTACCGTCC
GCCCGGATGTGGTGGTGAATGCCGCCGCGCACACGGCGGTGGACAAGGCAGAAAGCGAGCGGGAGCTCTCCGACCTGCTG
AACGACAAAGGGGTGGCGGTGCTAGCGGCAGAGTCGGCGAAGCTGGGTGCCCTGATGGTGCACTATTCCACGGATTATGT
GTTCGACGGCGCCGGCAGCCATTATCGCCGGGAAGATGAGGCAACCAGCCCGCTGAACGTCTACGGCGAAACCAAACGCG
CCGGTGAACTGGCCCTGGAGCAGGGTAACCCGCGTCACCTGATTTTCCGTACCAGCTGGGTGTATGCCACCCGCGGCGCC
AACTTTGCCAAAACCATGCTCCGCCTGGCGGGTGAGAAAGAGACGCTCTCCATCATTGACGACCAGCACGGGGCGCCCAC
CGGCGCTGAGCTGCTGGCAGACTGCACGGCGATGGCAATCCGTGAAACGTTGCGTAATCCGGCGCTGGCCGGTACCTATC
ATCTGGTGGCCAGCGGCGAGACCAGCTGGTGCGACTACGCCCGCTATGTGTTTGAGGTGGCGAGGGCGCACGGTGCAGAG
CTGGCGGTTCAGGAAGTGAAGGGCATTCCGACGACGGCCTATCCGACGCCGGCGAAGCGTCCGCTCAACTCACGCCTGTC
GAATGAAAAATTCCAGCAGGCATTTGGGGTGACTCTCCCGGACTGGCGTCAGGGTGTGGCTCGCGTGGTAACAGAAGTCC
TGGGCAAATAA

Upstream 100 bases:

>100_bases
GCTCAGGCGGAACGCCTGAAGAAAACCGAGTACGGTGCTTATCTGAAGCGCCTGCTGAACGAGCGTTAATGCCTTCTTCG
TCAACGACAGGATACAGAGA

Downstream 100 bases:

>100_bases
ACGGGTGGCAAACGATGAACATTATTAAAACAGATATTCCTGAGGTGCTGATTTTTGAACCGCGCGTGTTTGGTGATGCG
CGGGGCTTCTTCTTCGAGAG

Product: dTDP-4-dehydrorhamnose reductase

Products: NA

Alternate protein names: dTDP-4-keto-L-rhamnose reductase; dTDP-6-deoxy-L-lyxo-4-hexulose reductase; dTDP-6-deoxy-L-mannose dehydrogenase; dTDP-L-rhamnose synthase [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MKILLIGKNGQVGWELQRSLSTLGDVVAVDYFDKELCGDLTNLEGIAQTVRTVRPDVVVNAAAHTAVDKAESERELSDLL
NDKGVAVLAAESAKLGALMVHYSTDYVFDGAGSHYRREDEATSPLNVYGETKRAGELALEQGNPRHLIFRTSWVYATRGA
NFAKTMLRLAGEKETLSIIDDQHGAPTGAELLADCTAMAIRETLRNPALAGTYHLVASGETSWCDYARYVFEVARAHGAE
LAVQEVKGIPTTAYPTPAKRPLNSRLSNEKFQQAFGVTLPDWRQGVARVVTEVLGK

Sequences:

>Translated_296_residues
MKILLIGKNGQVGWELQRSLSTLGDVVAVDYFDKELCGDLTNLEGIAQTVRTVRPDVVVNAAAHTAVDKAESERELSDLL
NDKGVAVLAAESAKLGALMVHYSTDYVFDGAGSHYRREDEATSPLNVYGETKRAGELALEQGNPRHLIFRTSWVYATRGA
NFAKTMLRLAGEKETLSIIDDQHGAPTGAELLADCTAMAIRETLRNPALAGTYHLVASGETSWCDYARYVFEVARAHGAE
LAVQEVKGIPTTAYPTPAKRPLNSRLSNEKFQQAFGVTLPDWRQGVARVVTEVLGK
>Mature_296_residues
MKILLIGKNGQVGWELQRSLSTLGDVVAVDYFDKELCGDLTNLEGIAQTVRTVRPDVVVNAAAHTAVDKAESERELSDLL
NDKGVAVLAAESAKLGALMVHYSTDYVFDGAGSHYRREDEATSPLNVYGETKRAGELALEQGNPRHLIFRTSWVYATRGA
NFAKTMLRLAGEKETLSIIDDQHGAPTGAELLADCTAMAIRETLRNPALAGTYHLVASGETSWCDYARYVFEVARAHGAE
LAVQEVKGIPTTAYPTPAKRPLNSRLSNEKFQQAFGVTLPDWRQGVARVVTEVLGK

Specific function: Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4- hexulose to yield dTDP-L-rhamnose. RmlD uses NADH and NADPH nearly equally well [H]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI11034825, Length=239, Percent_Identity=26.3598326359833, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI33519455, Length=239, Percent_Identity=26.3598326359833, Blast_Score=82, Evalue=4e-16,
Organism=Escherichia coli, GI1788352, Length=294, Percent_Identity=61.2244897959184, Blast_Score=379, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: =1.1.1.133 [H]

Molecular weight: Translated: 32234; Mature: 32234

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILLIGKNGQVGWELQRSLSTLGDVVAVDYFDKELCGDLTNLEGIAQTVRTVRPDVVVN
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEE
AAAHTAVDKAESERELSDLLNDKGVAVLAAESAKLGALMVHYSTDYVFDGAGSHYRREDE
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEECCCEEECCCCCCCCCCCC
ATSPLNVYGETKRAGELALEQGNPRHLIFRTSWVYATRGANFAKTMLRLAGEKETLSIID
CCCCCEECCCCCCHHHHHCCCCCCCEEEEEEEEEEEECCCHHHHHHHHHCCCCCCEEEEC
DQHGAPTGAELLADCTAMAIRETLRNPALAGTYHLVASGETSWCDYARYVFEVARAHGAE
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCHH
LAVQEVKGIPTTAYPTPAKRPLNSRLSNEKFQQAFGVTLPDWRQGVARVVTEVLGK
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKILLIGKNGQVGWELQRSLSTLGDVVAVDYFDKELCGDLTNLEGIAQTVRTVRPDVVVN
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEE
AAAHTAVDKAESERELSDLLNDKGVAVLAAESAKLGALMVHYSTDYVFDGAGSHYRREDE
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEECCCEEECCCCCCCCCCCC
ATSPLNVYGETKRAGELALEQGNPRHLIFRTSWVYATRGANFAKTMLRLAGEKETLSIID
CCCCCEECCCCCCHHHHHCCCCCCCEEEEEEEEEEEECCCHHHHHHHHHCCCCCCEEEEC
DQHGAPTGAELLADCTAMAIRETLRNPALAGTYHLVASGETSWCDYARYVFEVARAHGAE
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCHH
LAVQEVKGIPTTAYPTPAKRPLNSRLSNEKFQQAFGVTLPDWRQGVARVVTEVLGK
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609 [H]