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The map label for this gene is ydfJ

Identifier: 157161019

GI number: 157161019

Start: 1654071

End: 1655354

Strand: Reverse

Name: ydfJ

Synonym: EcHS_A1633

Alternate gene names: 157161019

Gene position: 1655354-1654071 (Counterclockwise)

Preceding gene: 157161022

Following gene: 157161018

Centisome position: 35.65

GC content: 46.88

Gene sequence:

>1284_bases
ATGGATTTCCAGTTATATTCGCTCGGCGCAGCGTTAGTGTTTCATGAAATATTTTTTCCTGAATCATCAACGGCAATGGC
GTTAATTCTGGCAATGGGAACCTACGGTGCAGGTTATGTGGCGCGTATTGTCGGAGCATTTATTTTCGGCAAAATGGGCG
ACAGAATAGGGCGTAAAAAAGTGCTCTTTATTACCATCACCATGATGGGGATCTGTACCACCTTAATTGGTGTGTTACCG
ACCTATGCACAGATTGGTGTTTTTGCCCCCATCTTGCTGGTGACGCTGCGTATTATTCAGGGATTGGGTGCAGGTGCGGA
AATTTCCGGTGCCGGTACGATGCTGGCGGAATATGCGCCAAAAGGTAAGCGCGGAATTATCTCCTCATTTGTGGCTATGG
GAACTAACTGCGGAACTTTAAGCGCAACGGCAATCTGGGCCTTTATGTTCTTCATTCTCAGTAAAGAGGAACTGCTGGCG
TGGGGATGGCGTATACCGTTCCTGGCGAGCGTTGTCGTGATGGTCTTTGCTATCTGGTTGCGTATGAATCTGAAAGAAAG
CCCGGTTTTTGAGAAGGTTAACGACAGCAACCAACCGACAGCAAAACCTGCACCTGCTGGTAGCATGTTCCAGAGCAAAT
CCTTCTGGCTGGCAACAGGGCTGCGTTTTGGTCAGGCGGGTAACTCAGGTTTAATTCAGACTTTCCTTGCGGGCTATTTA
GTGCAGACGTTATTGTTTAACAAAGCAATTCCAACAGATGCATTGATGATCAGTTCGATTCTCGGCTTTATGACCATTCC
GTTCCTTGGTTGGTTATCCGATAAAATTGGTCGCCGGATCCCGTATATTATTATGAATACCTCCGCGATTGTGCTGGCAT
GGCCAATGCTTTCTATCATCGTAGATAAAAGCTATGCCCCGAGCACCATTATGGTTGCACTGATTGTGATTCATAACTGT
GCGGTGCTGGGATTATTTGCTCTGGAAAATATTACCATGGCAGAAATGTTCGGCTGTAAAAACCGCTTTACCCGGATGGC
TATTTCTAAAGAAATTGGTGGTCTTATCGCTTCCGGTTTTGGTCCTATCCTGGCGGGTATTTTCTGCACCATGACGGAAT
CCTGGTATCCGATCGCCATTATGATCATGGCATATTCAGTGATTGGTTTAATCTCTGCGCTGAAAATGCCAGAAGTGAAA
GACCGTGATTTAAGTGCGCTGGAAGACGCCGCGGAAGATCAACCGCGTGTTGTAAGAGCTGCGCAACCTTCCAGAAGTCT
GTAA

Upstream 100 bases:

>100_bases
CTGAGATCGAACTTAGGATGCTTTTAAAAAAATGCAACTATCTGAAAAAACCTAGAAAACGCCAAGGAAACCACAGGATG
GGAAAAAACACCTGTGAATT

Downstream 100 bases:

>100_bases
ATCCTCAATCCCTTCTCTTACCGGAGAGGGGATTTTTATTCATATAACAAAACATGTAGCTTGCCATATTTATATTTAAG
GAATTGTTATGGGAAATAAT

Product: inner membrane metabolite transport protein ydfJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MDFQLYSLGAALVFHEIFFPESSTAMALILAMGTYGAGYVARIVGAFIFGKMGDRIGRKKVLFITITMMGICTTLIGVLP
TYAQIGVFAPILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMGTNCGTLSATAIWAFMFFILSKEELLA
WGWRIPFLASVVVMVFAIWLRMNLKESPVFEKVNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYL
VQTLLFNKAIPTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPSTIMVALIVIHNC
AVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAGIFCTMTESWYPIAIMIMAYSVIGLISALKMPEVK
DRDLSALEDAAEDQPRVVRAAQPSRSL

Sequences:

>Translated_427_residues
MDFQLYSLGAALVFHEIFFPESSTAMALILAMGTYGAGYVARIVGAFIFGKMGDRIGRKKVLFITITMMGICTTLIGVLP
TYAQIGVFAPILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMGTNCGTLSATAIWAFMFFILSKEELLA
WGWRIPFLASVVVMVFAIWLRMNLKESPVFEKVNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYL
VQTLLFNKAIPTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPSTIMVALIVIHNC
AVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAGIFCTMTESWYPIAIMIMAYSVIGLISALKMPEVK
DRDLSALEDAAEDQPRVVRAAQPSRSL
>Mature_427_residues
MDFQLYSLGAALVFHEIFFPESSTAMALILAMGTYGAGYVARIVGAFIFGKMGDRIGRKKVLFITITMMGICTTLIGVLP
TYAQIGVFAPILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMGTNCGTLSATAIWAFMFFILSKEELLA
WGWRIPFLASVVVMVFAIWLRMNLKESPVFEKVNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYL
VQTLLFNKAIPTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPSTIMVALIVIHNC
AVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAGIFCTMTESWYPIAIMIMAYSVIGLISALKMPEVK
DRDLSALEDAAEDQPRVVRAAQPSRSL

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family

Homologues:

Organism=Escherichia coli, GI1788292, Length=402, Percent_Identity=33.8308457711443, Blast_Score=228, Evalue=8e-61,
Organism=Escherichia coli, GI1790550, Length=426, Percent_Identity=30.0469483568075, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI1789941, Length=416, Percent_Identity=29.0865384615385, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1788942, Length=369, Percent_Identity=31.9783197831978, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI87082231, Length=310, Percent_Identity=23.8709677419355, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI87082404, Length=273, Percent_Identity=26.3736263736264, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YDFJ_ECOLI (P77228)

Other databases:

- EMBL:   U00096
- EMBL:   AP009048
- PIR:   B64909
- RefSeq:   AP_002164.1
- ProteinModelPortal:   P77228
- SMR:   P77228
- DIP:   DIP-11699N
- EnsemblBacteria:   EBESCT00000017089
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW1536
- EchoBASE:   EB3583
- EcoGene:   EG13822
- GeneTree:   EBGT00050000008829
- HOGENOM:   HBG757988
- OMA:   LVTLRIV
- BioCyc:   EcoCyc:B1543-MONOMER
- Genevestigator:   P77228
- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- TIGRFAMs:   TIGR00883

Pfam domain/function: PF00083 Sugar_tr; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 46268; Mature: 46268

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: PS50850 MFS; PS00216 SUGAR_TRANSPORT_1; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x14dd298c)-; HASH(0x11a1b8ac)-; HASH(0x14145a9c)-; HASH(0x14dfd6a0)-; HASH(0x14509a04)-; HASH(0x14c84698)-; HASH(0x14170d24)-; HASH(0x1454fef0)-; HASH(0x14088438)-; HASH(0x13f4ff04)-; HASH(0x148a9bd4)-; HASH(0x14dbb880)-;

Cys/Met content:

1.2 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFQLYSLGAALVFHEIFFPESSTAMALILAMGTYGAGYVARIVGAFIFGKMGDRIGRKK
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCE
VLFITITMMGICTTLIGVLPTYAQIGVFAPILLVTLRIIQGLGAGAEISGAGTMLAEYAP
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCHHHHHHHCC
KGKRGIISSFVAMGTNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWL
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RMNLKESPVFEKVNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYL
HCCCCCCCCHHHCCCCCCCCCCCCCCCCHHCCCCHHHHHCCHHCCCCCCHHHHHHHHHHH
VQTLLFNKAIPTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSII
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHH
VDKSYAPSTIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGF
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GPILAGIFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCEEEC
AQPSRSL
CCCCCCH
>Mature Secondary Structure
MDFQLYSLGAALVFHEIFFPESSTAMALILAMGTYGAGYVARIVGAFIFGKMGDRIGRKK
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCE
VLFITITMMGICTTLIGVLPTYAQIGVFAPILLVTLRIIQGLGAGAEISGAGTMLAEYAP
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCHHHHHHHCC
KGKRGIISSFVAMGTNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWL
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RMNLKESPVFEKVNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYL
HCCCCCCCCHHHCCCCCCCCCCCCCCCCHHCCCCHHHHHCCHHCCCCCCHHHHHHHHHHH
VQTLLFNKAIPTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSII
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHH
VDKSYAPSTIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGF
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GPILAGIFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCEEEC
AQPSRSL
CCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503