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The map label for this gene is ydfI [H]

Identifier: 157161018

GI number: 157161018

Start: 1652522

End: 1653982

Strand: Reverse

Name: ydfI [H]

Synonym: EcHS_A1632

Alternate gene names: 157161018

Gene position: 1653982-1652522 (Counterclockwise)

Preceding gene: 157161019

Following gene: 157161017

Centisome position: 35.62

GC content: 49.9

Gene sequence:

>1461_bases
ATGGGAAATAATTTGTTATCAGCAAAAGCGACACTCCCTGTTTATGATCGTAATAACCTGGCCCCAAGAATTGTTCATTT
AGGCTTTGGTGCATTTCACCGTGCGCATCAGGGCGTGTATGCCGATATTCTTGCTACAGAACATTTCAGTGACTGGGGAT
ATTATGAGGTCAACTTAATCGGCGGCGAACAGCAAATTGCCGATTTACAGCAGCAAGATAATCTTTATACCGTTGCGGAA
ATGTCGGCCGATGCGTGGACGGCTCGCGTCGTTGGCGTCGTTAAAAAAGCCTTGCACGTACAGATGGATGGCTTAGAAAC
CGTGTTGGCTGCGATGTGTGAACCGCAAATCGCGATTGTCTCTCTGACAATCACCGAAAAAGGGTATTTCCACTCTCCGG
CGACCGGACAGTTAATGCTCGATCACCCGATGGTCGCTGCCGACGTGCAAAATCCCCACCAGCCGAAAACAGCAACAGGG
GTGATTGTTGAGGCGCTGGCTCGCCGTAAAGCGGCAGGACTTCCCGCATTTACCGTCATGTCATGTGACAACATGCCAGA
AAACGGTCATGTTATGCGTGACGTTGTCACTTCCTACGCGCAAGCCGTTGATGTAAAACTGGCACAATGGATCGAAGATA
ACGTGACTTTCCCATCAACAATGGTGGACCGTATTGTGCCCGCAGTGACAGAGGATACGCTGGCGAAAATCGAACAACTT
ACCGGTGTGCGCGATCCTGCTGGCGTTGCCTGTGAACCTTTCCGCCAGTGGGTAATAGAAGATAATTTTGTTGCCGGACG
TCCGGAATGGGAAAAAGCGGGAGCAGAACTGGTTAGCGATGTACTGCCTTATGAAGAGATGAAGTTGCGCATGCTCAACG
GCAGTCATTCATTCCTGGCGTATTTGGGTTATCTTGCCGGATATCAGCACATTAATGACTGTATGGAAGATGAACATTAT
CGTCATGCGGCGTATGGCTTGATGTTGCAGGAACAAGCGCCGACGCTGAAAGTGCAGGGCGTTGATTTGCAGGATTACGC
TAACCGATTAATTGCACGCTATAGCAACCCGGCGTTACGTCATCGAACCTGGCAGATTGCGATGGATGGCAGCCAGAAAT
TGCCACAGCGGATGTTGGATTCTGTTCGCTGGCATCTGACGCATGACAGCAAGTTCGATCTGCTGGCGCTGGGCGTCGCG
GGTTGGATGCGTTATGTCGGTGGTGTTGATGAACAGGAAAATCCGATAGAAATCAGTGACCCACTGTTACCTGTTATTCA
GAAGGCTGTACAAAGTAGTGCCGAAGGGAAAGCGCGCGTCCAGTCATTGCTGGCGATTAAGGCGATCTTTGGTGATGATT
TGCCAGCCAATAGCTTGTTTACTGCAAAAGTGACGGAAGCGTACTTGTCTTTATTAGCGCATGGCGCGAAAGCGACCGTG
GCAAAATATTCCGTGAAGTAA

Upstream 100 bases:

>100_bases
AGAAGTCTGTAAATCCTCAATCCCTTCTCTTACCGGAGAGGGGATTTTTATTCATATAACAAAACATGTAGCTTGCCATA
TTTATATTTAAGGAATTGTT

Downstream 100 bases:

>100_bases
AAAAAGCAATGCCGCCATCGGCGGCATTCACACGCTTAGTTCATGCCGAGACGAATCAGGTCCAGTGGTGCCAGTTTCTC
TTCACAACCTTCAACAACTA

Product: mannitol dehydrogenase family protein

Products: D-fructuronate; NADH [C]

Alternate protein names: NA

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADILATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAE
MSADAWTARVVGVVKKALHVQMDGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFPSTMVDRIVPAVTEDTLAKIEQL
TGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAELVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHY
RHAAYGLMLQEQAPTLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLTHDSKFDLLALGVA
GWMRYVGGVDEQENPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIFGDDLPANSLFTAKVTEAYLSLLAHGAKATV
AKYSVK

Sequences:

>Translated_486_residues
MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADILATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAE
MSADAWTARVVGVVKKALHVQMDGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFPSTMVDRIVPAVTEDTLAKIEQL
TGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAELVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHY
RHAAYGLMLQEQAPTLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLTHDSKFDLLALGVA
GWMRYVGGVDEQENPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIFGDDLPANSLFTAKVTEAYLSLLAHGAKATV
AKYSVK
>Mature_485_residues
GNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADILATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEM
SADAWTARVVGVVKKALHVQMDGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATGV
IVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFPSTMVDRIVPAVTEDTLAKIEQLT
GVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAELVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYR
HAAYGLMLQEQAPTLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLTHDSKFDLLALGVAG
WMRYVGGVDEQENPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIFGDDLPANSLFTAKVTEAYLSLLAHGAKATVA
KYSVK

Specific function: Unknown

COG id: COG0246

COG function: function code G; Mannitol-1-phosphate/altronate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannitol dehydrogenase family. UxuB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787823, Length=486, Percent_Identity=98.1481481481482, Blast_Score=990, Evalue=0.0,
Organism=Escherichia coli, GI1790779, Length=472, Percent_Identity=57.8389830508475, Blast_Score=550, Evalue=1e-158,
Organism=Escherichia coli, GI1788497, Length=464, Percent_Identity=54.7413793103448, Blast_Score=547, Evalue=1e-157,
Organism=Escherichia coli, GI48994885, Length=368, Percent_Identity=26.9021739130435, Blast_Score=112, Evalue=7e-26,
Organism=Escherichia coli, GI1790028, Length=260, Percent_Identity=28.0769230769231, Blast_Score=97, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324401, Length=488, Percent_Identity=39.9590163934426, Blast_Score=355, Evalue=1e-98,
Organism=Saccharomyces cerevisiae, GI6320765, Length=488, Percent_Identity=39.9590163934426, Blast_Score=355, Evalue=1e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR013328
- InterPro:   IPR000669
- InterPro:   IPR013118
- InterPro:   IPR023027
- InterPro:   IPR013131
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01232 Mannitol_dh; PF08125 Mannitol_dh_C [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 53693; Mature: 53562

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS00974 MANNITOL_DHGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADILATEHFSDWGYYEVNLI
CCCCEEECCCEEEEECCCCCCCEEEEECHHHHHHHHCCHHHHHHHHHCCCCCCEEEEEEE
GGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQMDGLETVLAAMCEPQIAIV
CCHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCEEEE
SLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATGVIVEALARRKAAGLPAFTVM
EEEEECCCCCCCCCCCCEEECCCEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEE
SCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFPSTMVDRIVPAVTEDTLAKIEQL
ECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHH
TGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAELVSDVLPYEEMKLRMLNGSHSFLA
HCCCCCCCCCHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHCCHHHHHEEEECCCHHHHH
YLGYLAGYQHINDCMEDEHYRHAAYGLMLQEQAPTLKVQGVDLQDYANRLIARYSNPALR
HHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEECCCHHHHHHHHHHHCCCCHHH
HRTWQIAMDGSQKLPQRMLDSVRWHLTHDSKFDLLALGVAGWMRYVGGVDEQENPIEISD
CEEEEEEECCHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCEECCC
PLLPVIQKAVQSSAEGKARVQSLLAIKAIFGDDLPANSLFTAKVTEAYLSLLAHGAKATV
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHCCCHHHH
AKYSVK
EEECCC
>Mature Secondary Structure 
GNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADILATEHFSDWGYYEVNLI
CCCEEECCCEEEEECCCCCCCEEEEECHHHHHHHHCCHHHHHHHHHCCCCCCEEEEEEE
GGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQMDGLETVLAAMCEPQIAIV
CCHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCEEEE
SLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATGVIVEALARRKAAGLPAFTVM
EEEEECCCCCCCCCCCCEEECCCEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEE
SCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFPSTMVDRIVPAVTEDTLAKIEQL
ECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHH
TGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAELVSDVLPYEEMKLRMLNGSHSFLA
HCCCCCCCCCHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHCCHHHHHEEEECCCHHHHH
YLGYLAGYQHINDCMEDEHYRHAAYGLMLQEQAPTLKVQGVDLQDYANRLIARYSNPALR
HHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEECCCHHHHHHHHHHHCCCCHHH
HRTWQIAMDGSQKLPQRMLDSVRWHLTHDSKFDLLALGVAGWMRYVGGVDEQENPIEISD
CEEEEEEECCHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCEECCC
PLLPVIQKAVQSSAEGKARVQSLLAIKAIFGDDLPANSLFTAKVTEAYLSLLAHGAKATV
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHCCCHHHH
AKYSVK
EEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-mannonate; NAD(+) [C]

Specific reaction: D-mannonate + NAD(+) = D-fructuronate + NADH [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]