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The map label for this gene is fixA

Identifier: 157159512

GI number: 157159512

Start: 45663

End: 46433

Strand: Direct

Name: fixA

Synonym: EcHS_A0047

Alternate gene names: 157159512

Gene position: 45663-46433 (Clockwise)

Preceding gene: 157159511

Following gene: 157159513

Centisome position: 0.98

GC content: 52.4

Gene sequence:

>771_bases
ATGAAGATTATTACTTGCTATAAGTGCGTGCCTGATGAACAGGATATTGCGGTCAATAATGCTGATGGTTCATTAGACTT
CAGCAAAGCCGATGCCAAAATAAGCCAATACGATCTGAACGCTATTGAAGCGGCTTGCCAGCTAAAGCAACAGGCAGCAG
AGGCGCAGGTGACGGCCTTAAGTGTGGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGC
CCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGCAACAAACGGCGAGCGCACTGGCTGCAGC
CGCCCAGAAAGCAGGCTTTGATTTGATCCTCTGTGGCGATGGTTCTTCCGACCTTTATGCCCAGCAGGTTGGTCTGCTGG
TGGGCGAAATCCTCAATATTCCGGCAGTTAACGGCGTCAGCAAAATTATCTCCCTGACTGCAGATACCCTCACCGTTGAG
CGCGAACTGGAAGATGAAACCGAAACCTTAAGCATTCCGCTGCCTGCGGTTGTTGCTGTTTCCACTGATATCAACTCCCC
ACAAATTCCTTCGATGAAAGCCATTCTCGGCGCGGCGAAAAAGCCCGTCCAGGTATGGTCGGCGGCGGATATTGGTTTTA
ACGCAGAGGCAGCCTGGTCAGAACAACAGGTTGCCGCGCCGAAACAGCGCGAACGTCAGCGCATCGTGATTGAAGGCGAC
GGCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAA

Upstream 100 bases:

>100_bases
TATTGGTGATTAAAGTTTTATTTCCAAATTAAAGGGCGTGATATCTGTAATTAACACCACCGATATGAACGACGTTTCCT
TCATGATTTCTGGAGATGCA

Downstream 100 bases:

>100_bases
TTACAGGGGATGCTATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAAC
GGTGCGCAGGCTTTAGCTAA

Product: putative electron transfer flavoprotein FixA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEAACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRG
PDELIVVIDDQFEQALPQQTASALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAAWSEQQVAAPKQRERQRIVIEGD
GEEQIAAFAENLRKVI

Sequences:

>Translated_256_residues
MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEAACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRG
PDELIVVIDDQFEQALPQQTASALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAAWSEQQVAAPKQRERQRIVIEGD
GEEQIAAFAENLRKVI
>Mature_256_residues
MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEAACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRG
PDELIVVIDDQFEQALPQQTASALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAAWSEQQVAAPKQRERQRIVIEGD
GEEQIAAFAENLRKVI

Specific function: Required for anaerobic carnitine reduction. May bring reductant to CaiA

COG id: COG2086

COG function: function code C; Electron transfer flavoprotein, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ETF beta-subunit/fixA family

Homologues:

Organism=Homo sapiens, GI4503609, Length=192, Percent_Identity=31.7708333333333, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI62420877, Length=192, Percent_Identity=31.7708333333333, Blast_Score=75, Evalue=6e-14,
Organism=Escherichia coli, GI87081682, Length=256, Percent_Identity=100, Blast_Score=510, Evalue=1e-146,
Organism=Escherichia coli, GI2367123, Length=257, Percent_Identity=55.6420233463035, Blast_Score=269, Evalue=1e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FIXA_ECO24 (A7ZHD4)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001461213.1
- ProteinModelPortal:   A7ZHD4
- SMR:   A7ZHD4
- STRING:   A7ZHD4
- EnsemblBacteria:   EBESCT00000022435
- GeneID:   5589423
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_0045
- eggNOG:   COG2086
- GeneTree:   EBGT00050000010718
- HOGENOM:   HBG716515
- OMA:   ARKDILS
- ProtClustDB:   PRK03359
- BioCyc:   ECOL331111:ECE24377A_0045-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01055
- InterPro:   IPR000049
- InterPro:   IPR014730
- InterPro:   IPR012255
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR21294
- PIRSF:   PIRSF000090
- SMART:   SM00893

Pfam domain/function: PF01012 ETF

EC number: NA

Molecular weight: Translated: 27144; Mature: 27144

Theoretical pI: Translated: 4.21; Mature: 4.21

Prosite motif: PS01065 ETF_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEAACQLKQQAAEAQVTAL
CCEEEEEECCCCCCCEEEECCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHEEEE
SVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTASALAAAAQKAGFDLILCGD
ECCCHHHCCCCCHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCEEEEECC
GSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVERELEDETETLSIPLPAVVAV
CCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEEEHHHCCCCCEEECCCCEEEEE
STDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAAWSEQQVAAPKQRERQRIVIEGD
ECCCCCCCCCHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCHHHCCCHHHCCCEEEEECC
GEEQIAAFAENLRKVI
CHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEAACQLKQQAAEAQVTAL
CCEEEEEECCCCCCCEEEECCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHEEEE
SVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTASALAAAAQKAGFDLILCGD
ECCCHHHCCCCCHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCEEEEECC
GSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVERELEDETETLSIPLPAVVAV
CCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEEEHHHCCCCCEEECCCCEEEEE
STDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAAWSEQQVAAPKQRERQRIVIEGD
ECCCCCCCCCHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCHHHCCCHHHCCCEEEEECC
GEEQIAAFAENLRKVI
CHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA