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The map label for this gene is fixB [H]

Identifier: 157159513

GI number: 157159513

Start: 46448

End: 47389

Strand: Direct

Name: fixB [H]

Synonym: EcHS_A0048

Alternate gene names: 157159513

Gene position: 46448-47389 (Clockwise)

Preceding gene: 157159512

Following gene: 157159514

Centisome position: 1.0

GC content: 56.16

Gene sequence:

>942_bases
ATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCTTT
AGCTAATCAAATCAACACCTTTGTCCTCAATGATGCCGACGGCGTACAGGCAATCCAGCTCGGCGCTAATCATGTCTGGA
AATTAAACGGCAAACCGGACGATCGGATGATCGAAGATTACGCCGGTGTCATGGCTGACACTATTCGCCAGCACGGCGCA
GACGGCCTGGTGCTGCTGCCAAACACCCGTCGCGGCAAATTACTGGCGGCAAAACTGGGTTATCGCCTTAAAGCGGCGGT
GTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTG
GCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCATCAGCAGCGGCACGTTCGATGCGGCTCAGCCAGACGCGTCA
CGCACTGGCGAAACGCACACCGTGGAGTGGCAGGCTCCGGCTGTGGCGATTACCCGCACGGCAACCCAGGCGCGCCAGAG
CAACAGCGTCGATCTCGACAAAGCCCGTCTGGTGGTCAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGG
CAGAACAGCTTTGCAAGGCGATAGGTGCGGAGTTGGCCTGTTCTCGTCCGGTGGCGGAAAACGAAAAATGGATGGAGCAC
GAACGCTATGTCGGTATCTCCAACCTGATGCTGAAACCTGAACTGTACCTGGCGGTGGGGATCTCCGGGCAGATCCAGCA
CATGGTTGGTGCTAATGCATCGCAAACCATTTTCGCCATCAATAAAGATAAAAATGCGCCGATCTTCCAGTACGCGGATT
ACGGCATTGTTGGTGACGCCGTGAAGATCCTTCCGGCGCTGACCGCAGCTTTAGCGCGTTGA

Upstream 100 bases:

>100_bases
AGCGCGAACGTCAGCGCATCGTGATTGAAGGCGACGGCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTC
ATTTAATTACAGGGGATGCT

Downstream 100 bases:

>100_bases
TCCACTCTGGCAGGGCTGCATTTTGGCCCTGCCGCTGACAGGGAGCTCTTATGTCCGAAGATATCTTTGACGCCATCATC
GTCGGTGCTGGGCTTGCCGG

Product: putative electron transfer flavoprotein FixB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGVQAIQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGA
DGLVLLPNTRRGKLLAAKLGYRLKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLCKAIGAELACSRPVAENEKWMEH
ERYVGISNLMLKPELYLAVGISGQIQHMVGANASQTIFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR

Sequences:

>Translated_313_residues
MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGVQAIQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGA
DGLVLLPNTRRGKLLAAKLGYRLKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLCKAIGAELACSRPVAENEKWMEH
ERYVGISNLMLKPELYLAVGISGQIQHMVGANASQTIFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR
>Mature_313_residues
MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGVQAIQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGA
DGLVLLPNTRRGKLLAAKLGYRLKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLCKAIGAELACSRPVAENEKWMEH
ERYVGISNLMLKPELYLAVGISGQIQHMVGANASQTIFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR

Specific function: Required for anaerobic carnitine reduction. May bring reductant to CaiA [H]

COG id: COG2025

COG function: function code C; Electron transfer flavoprotein, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ETF alpha-subunit/fixB family [H]

Homologues:

Organism=Homo sapiens, GI189181759, Length=234, Percent_Identity=35.042735042735, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI4503607, Length=234, Percent_Identity=35.042735042735, Blast_Score=115, Evalue=4e-26,
Organism=Escherichia coli, GI1786226, Length=313, Percent_Identity=99.6805111821086, Blast_Score=642, Evalue=0.0,
Organism=Escherichia coli, GI1787990, Length=314, Percent_Identity=43.9490445859873, Blast_Score=273, Evalue=1e-74,
Organism=Escherichia coli, GI87082157, Length=116, Percent_Identity=38.7931034482759, Blast_Score=66, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17506929, Length=225, Percent_Identity=33.7777777777778, Blast_Score=111, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6325261, Length=235, Percent_Identity=30.6382978723404, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI17136898, Length=195, Percent_Identity=38.4615384615385, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24652801, Length=195, Percent_Identity=38.4615384615385, Blast_Score=112, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001308
- InterPro:   IPR014730
- InterPro:   IPR014731
- InterPro:   IPR018206
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01012 ETF; PF00766 ETF_alpha [H]

EC number: NA

Molecular weight: Translated: 33542; Mature: 33542

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: PS00696 ETF_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGVQAIQLGANHVWKLNGKPD
CCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEEECCCEEEEECCCCC
DRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYRLKAAVSNDASTVSVQDGK
HHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCEEEEHHCCEEEEEECCCCCEEEEECCH
ATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDASRTGETHTVEWQAPAVAITRT
HHHHHHHHCCEEECCHHCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEEEHH
ATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLCKAIGAELACSRPVAENEKWMEH
HHHHHCCCCCCHHHHHEEEEECCCCCCCCCHHHHHHHHHHHCCHHCCCCCCCCCHHHHHH
ERYVGISNLMLKPELYLAVGISGQIQHMVGANASQTIFAINKDKNAPIFQYADYGIVGDA
HHCCCHHHEEECCCEEEEEECCCHHHHHHCCCCCCEEEEEECCCCCCEEEECCCCCHHHH
VKILPALTAALAR
HHHHHHHHHHHCC
>Mature Secondary Structure
MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGVQAIQLGANHVWKLNGKPD
CCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEEECCCEEEEECCCCC
DRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYRLKAAVSNDASTVSVQDGK
HHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCEEEEHHCCEEEEEECCCCCEEEEECCH
ATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDASRTGETHTVEWQAPAVAITRT
HHHHHHHHCCEEECCHHCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEEEHH
ATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLCKAIGAELACSRPVAENEKWMEH
HHHHHCCCCCCHHHHHEEEEECCCCCCCCCHHHHHHHHHHHCCHHCCCCCCCCCHHHHHH
ERYVGISNLMLKPELYLAVGISGQIQHMVGANASQTIFAINKDKNAPIFQYADYGIVGDA
HHCCCHHHEEECCCEEEEEECCCHHHHHHCCCCCCEEEEEECCCCCCEEEECCCCCHHHH
VKILPALTAALAR
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA