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The map label for this gene is thrS [H]

Identifier: 156978155

GI number: 156978155

Start: 2053794

End: 2054978

Strand: Reverse

Name: thrS [H]

Synonym: VIBHAR_06960

Alternate gene names: 156978155

Gene position: 2054978-2053794 (Counterclockwise)

Preceding gene: 156978156

Following gene: 156978154

Centisome position: 93.24

GC content: 41.1

Gene sequence:

>1185_bases
ATGAAAACACATCGTGAAATTGCAGAACAACTCGACTTATTCCATTCAGAACCACAGGCTCCAGGAATGATCTTTTGGCA
CCCGAATGGATGGCGCCTTTTTGAAAGTATTAAGAGCCACATGAGAAGTGTTTACCACAGAAGTGGGTTTCAAGAAGTAA
ATACACCAGTTTTCATGAAGCAGGAATTGTGGCAAAAGTCAGGTCACATTGATATGTTTTCTGAGAATATGTTCTTTGGC
GGGAACCAAGAACAACAGAGCGGATACGTGCTTAAGCCCATGTCTTGTCCTGCTCATATATTGCTATATAAACGTGGAGT
TCATAGCCATAAAGACCTACCTATTAAGTTATTTGAATTTGGACTAGTTCATCGTAATGAGTCATCCGGTTCACTGAATG
GCTGTTTACGAGCTAGGCAATTTACTCAAGATGATGCTCATGTCTTTTGCCAGTGGGAGCAAGCCGAAGATGAAGTTCGT
CAGTTCCTACAGAGAGCTAGAAAGGTATATGCCGCCTATGGATATGAACATATTGAAGTTAAGGTGTCGACTAAGCCAGA
AAAAGCGCTTGGTGATGACGAAGACTGGATAAGAGCGCAAGATATTCTTGTCTCAGCTTGTATCCACGAAAAATTCCATA
CTGAGTTGCAAGATGGCGAAGGTGCATTCTATGGACCGAAGATTGAGCTGTCTCTAACTGACTCAATGGGAAGAAGCTGG
CAGTGTGGAACGATCCAATTTGATTTCAATCTTGCGAAAAGGTTCGATGTTAAGTTTACAAGCGAAGAAGGGACCTTCGA
AGCACCTGTCATCATGCATCAAGCAATGTATGGCTCTGTAGAGCGTTGGATTGGGATTTTACTCGAAATGTCGCAAGGAG
ATTTGCCTTTATGGTTACACCCGCAACCAGTCGCGATAGCCTCCGTCAATCATTCAAATTGTCATTTCGCGAAAATTGTA
GCAGATAGGCTACTTGATATAGACATACATGCCTTATTGGATTTGAGTAAAAGTTCTATGGGAAGAAAAATGAAACGATT
CCACAAAAAGAAGATCCCTTTTGTTATCAGTATTGGTGACTACGAAGTTGAGAATGATGTGGTATCGATTAAAAATAGAA
AAGAAGCTAGTGAGACTCAAGTATCTATGGAGCACTTGGCAGAATATATTTTATCCAGCTTATGA

Upstream 100 bases:

>100_bases
TAGTTTTTCGCGTCTATAAAAAACCTCTGAGTGAATCTTCGCCAGAGGTTTTTTTGTATCTAGGCTTTGTATTTCACTCA
GAATAAATTGGAGAAGAAAT

Downstream 100 bases:

>100_bases
TAAGAAACACTAGTTCTACGTGAACAAAAATGAAACCTCAACTATTAATCATAGGTAGTTGAGGTAACGAAGTTAGGAGA
AAGTATGAAATCTAAAGATG

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MKTHREIAEQLDLFHSEPQAPGMIFWHPNGWRLFESIKSHMRSVYHRSGFQEVNTPVFMKQELWQKSGHIDMFSENMFFG
GNQEQQSGYVLKPMSCPAHILLYKRGVHSHKDLPIKLFEFGLVHRNESSGSLNGCLRARQFTQDDAHVFCQWEQAEDEVR
QFLQRARKVYAAYGYEHIEVKVSTKPEKALGDDEDWIRAQDILVSACIHEKFHTELQDGEGAFYGPKIELSLTDSMGRSW
QCGTIQFDFNLAKRFDVKFTSEEGTFEAPVIMHQAMYGSVERWIGILLEMSQGDLPLWLHPQPVAIASVNHSNCHFAKIV
ADRLLDIDIHALLDLSKSSMGRKMKRFHKKKIPFVISIGDYEVENDVVSIKNRKEASETQVSMEHLAEYILSSL

Sequences:

>Translated_394_residues
MKTHREIAEQLDLFHSEPQAPGMIFWHPNGWRLFESIKSHMRSVYHRSGFQEVNTPVFMKQELWQKSGHIDMFSENMFFG
GNQEQQSGYVLKPMSCPAHILLYKRGVHSHKDLPIKLFEFGLVHRNESSGSLNGCLRARQFTQDDAHVFCQWEQAEDEVR
QFLQRARKVYAAYGYEHIEVKVSTKPEKALGDDEDWIRAQDILVSACIHEKFHTELQDGEGAFYGPKIELSLTDSMGRSW
QCGTIQFDFNLAKRFDVKFTSEEGTFEAPVIMHQAMYGSVERWIGILLEMSQGDLPLWLHPQPVAIASVNHSNCHFAKIV
ADRLLDIDIHALLDLSKSSMGRKMKRFHKKKIPFVISIGDYEVENDVVSIKNRKEASETQVSMEHLAEYILSSL
>Mature_394_residues
MKTHREIAEQLDLFHSEPQAPGMIFWHPNGWRLFESIKSHMRSVYHRSGFQEVNTPVFMKQELWQKSGHIDMFSENMFFG
GNQEQQSGYVLKPMSCPAHILLYKRGVHSHKDLPIKLFEFGLVHRNESSGSLNGCLRARQFTQDDAHVFCQWEQAEDEVR
QFLQRARKVYAAYGYEHIEVKVSTKPEKALGDDEDWIRAQDILVSACIHEKFHTELQDGEGAFYGPKIELSLTDSMGRSW
QCGTIQFDFNLAKRFDVKFTSEEGTFEAPVIMHQAMYGSVERWIGILLEMSQGDLPLWLHPQPVAIASVNHSNCHFAKIV
ADRLLDIDIHALLDLSKSSMGRKMKRFHKKKIPFVISIGDYEVENDVVSIKNRKEASETQVSMEHLAEYILSSL

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38202255, Length=397, Percent_Identity=37.27959697733, Blast_Score=275, Evalue=7e-74,
Organism=Homo sapiens, GI20070344, Length=389, Percent_Identity=36.5038560411311, Blast_Score=263, Evalue=2e-70,
Organism=Homo sapiens, GI38570111, Length=374, Percent_Identity=35.8288770053476, Blast_Score=262, Evalue=5e-70,
Organism=Escherichia coli, GI1788013, Length=373, Percent_Identity=41.2868632707775, Blast_Score=338, Evalue=5e-94,
Organism=Caenorhabditis elegans, GI71984184, Length=391, Percent_Identity=35.0383631713555, Blast_Score=271, Evalue=4e-73,
Organism=Caenorhabditis elegans, GI71984192, Length=391, Percent_Identity=35.0383631713555, Blast_Score=271, Evalue=4e-73,
Organism=Saccharomyces cerevisiae, GI6322112, Length=393, Percent_Identity=36.3867684478371, Blast_Score=282, Evalue=8e-77,
Organism=Saccharomyces cerevisiae, GI6322654, Length=411, Percent_Identity=31.8734793187348, Blast_Score=240, Evalue=2e-64,
Organism=Drosophila melanogaster, GI24583839, Length=387, Percent_Identity=34.8837209302326, Blast_Score=269, Evalue=2e-72,
Organism=Drosophila melanogaster, GI24583842, Length=386, Percent_Identity=34.4559585492228, Blast_Score=269, Evalue=2e-72,
Organism=Drosophila melanogaster, GI28574141, Length=386, Percent_Identity=34.4559585492228, Blast_Score=269, Evalue=2e-72,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 45433; Mature: 45433

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTHREIAEQLDLFHSEPQAPGMIFWHPNGWRLFESIKSHMRSVYHRSGFQEVNTPVFMK
CCCHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCHHCCCCEEEH
QELWQKSGHIDMFSENMFFGGNQEQQSGYVLKPMSCPAHILLYKRGVHSHKDLPIKLFEF
HHHHHCCCCEEEEECCEEECCCCCCCCCEEEECCCCCCEEEEEECCCCCCCCCCEEEEEE
GLVHRNESSGSLNGCLRARQFTQDDAHVFCQWEQAEDEVRQFLQRARKVYAAYGYEHIEV
EEEECCCCCCCHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCEEEEEE
KVSTKPEKALGDDEDWIRAQDILVSACIHEKFHTELQDGEGAFYGPKIELSLTDSMGRSW
EEECCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCEEEEEEECCCCCCE
QCGTIQFDFNLAKRFDVKFTSEEGTFEAPVIMHQAMYGSVERWIGILLEMSQGDLPLWLH
ECEEEEEECEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCEEEEC
PQPVAIASVNHSNCHFAKIVADRLLDIDIHALLDLSKSSMGRKMKRFHKKKIPFVISIGD
CCCEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECC
YEVENDVVSIKNRKEASETQVSMEHLAEYILSSL
EEECCCEEECCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKTHREIAEQLDLFHSEPQAPGMIFWHPNGWRLFESIKSHMRSVYHRSGFQEVNTPVFMK
CCCHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCHHCCCCEEEH
QELWQKSGHIDMFSENMFFGGNQEQQSGYVLKPMSCPAHILLYKRGVHSHKDLPIKLFEF
HHHHHCCCCEEEEECCEEECCCCCCCCCEEEECCCCCCEEEEEECCCCCCCCCCEEEEEE
GLVHRNESSGSLNGCLRARQFTQDDAHVFCQWEQAEDEVRQFLQRARKVYAAYGYEHIEV
EEEECCCCCCCHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCEEEEEE
KVSTKPEKALGDDEDWIRAQDILVSACIHEKFHTELQDGEGAFYGPKIELSLTDSMGRSW
EEECCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCEEEEEEECCCCCCE
QCGTIQFDFNLAKRFDVKFTSEEGTFEAPVIMHQAMYGSVERWIGILLEMSQGDLPLWLH
ECEEEEEECEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCEEEEC
PQPVAIASVNHSNCHFAKIVADRLLDIDIHALLDLSKSSMGRKMKRFHKKKIPFVISIGD
CCCEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECC
YEVENDVVSIKNRKEASETQVSMEHLAEYILSSL
EEECCCEEECCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA