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The map label for this gene is yfiN [H]

Identifier: 156978126

GI number: 156978126

Start: 2024849

End: 2025940

Strand: Direct

Name: yfiN [H]

Synonym: VIBHAR_06931

Alternate gene names: 156978126

Gene position: 2024849-2025940 (Clockwise)

Preceding gene: 156978125

Following gene: 156978127

Centisome position: 91.87

GC content: 44.87

Gene sequence:

>1092_bases
ATGAAAGTACAACCCAACGTTCCCAAGCAGGGACCTTTTTCATCCCGAAGCAAAACCTTACTTCTGATTTTTGCTTCCAC
ACTGCTGGTCGCCAATATCGTGCTACTGCAGGCAACGCGCGCACTGACCAAAACCTATTCGGATCAACAAAACCAAGCGA
CTTGGTTTTTATTTCATTTATCCAAAGAGTTATCGGAACTGGTTGGCGAAGCTAGGCGCCTAAACGAAAGCATCAATAAT
TTTGATAGCGCCGAATTACAATACGAACTCGCCTGGAGCCGCTTTGACCTTTTGATTCATAACAAAGAAGCTGATTCTTT
CCTCTCCCGAGAAGAGGTTCGTGTGTATTTTATTTCCTTGTTTGACCGCTTTAAGCAGCTTGAGCCCATTTTAGTCGAAG
CCAAAACAGGTAACAGCGTTGCGGCCAGTCAGTTTTATCGCGCAACACAAAACCTCTATATGGAGATGATTGATTACGTT
ACCCAGCACTTCCGTGTCGCGAGCCCGCTTTATAAGCAGCAACAGAAACAGGCCTATGACCTACTTCAAGCGCAATACCT
GTTGCTAGGCATTTTCGTTATCACGGTAGCCTTAATGACGTATTTCTATAAACAAGAATCGAAGTTCCACAAAGCGCTTG
CATTAAGCGACTCACTGACCGGCTTGGCTAACCGAAGCGCTTTGTTTATTGACCTACAAAACAAGCAGGAGTCAGGAACG
ACCTTTTCCCTGCTTTTGCTTGATTTGAACGGTTTTAAAAACATTAACGATACGCAAGGACACCAAGCGGGTGATATCGC
ATTGACCGAAGTCGCCGAGCGCTTGAAGCATATGGCTCTGGATAACTTTAAAGTCTATCGAATGGGTGGAGATGAATTTG
CAGTCGTGATTAATTCAACGGTTAAACAAGATATCGAAGAAGCGAAGCGTCACATTCATGTGGTGTTTGAGAAGCCTATT
TTTGCGTCTGACCAAGCAAGCTCTCTATCAACCAGCCTCGGCGCGGCATGCTACCCGCAAGACACCGACAACATTGACTT
CCTGATCAATTTAGCGGATAAGCGCATGTACAAAATGAAATTCCAACGTTAA

Upstream 100 bases:

>100_bases
GGCTGATCTACCCAGTCACAAAATACCCAGAGCTTGATGTCAGTTACTACCACTCCCAAATGGTTTGGCAAATCAATAGG
ATCAGTACGAAAGAAGAAGA

Downstream 100 bases:

>100_bases
ACGGATAACCATGATGAAAAACTTCATGCGAGCAGTGCTATTACTGCTAGTGATCGCGATAGGTGTGCTGGCTTGGGTGT
TTATCCCATCGCACGAAACT

Product: hypothetical protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MKVQPNVPKQGPFSSRSKTLLLIFASTLLVANIVLLQATRALTKTYSDQQNQATWFLFHLSKELSELVGEARRLNESINN
FDSAELQYELAWSRFDLLIHNKEADSFLSREEVRVYFISLFDRFKQLEPILVEAKTGNSVAASQFYRATQNLYMEMIDYV
TQHFRVASPLYKQQQKQAYDLLQAQYLLLGIFVITVALMTYFYKQESKFHKALALSDSLTGLANRSALFIDLQNKQESGT
TFSLLLLDLNGFKNINDTQGHQAGDIALTEVAERLKHMALDNFKVYRMGGDEFAVVINSTVKQDIEEAKRHIHVVFEKPI
FASDQASSLSTSLGAACYPQDTDNIDFLINLADKRMYKMKFQR

Sequences:

>Translated_363_residues
MKVQPNVPKQGPFSSRSKTLLLIFASTLLVANIVLLQATRALTKTYSDQQNQATWFLFHLSKELSELVGEARRLNESINN
FDSAELQYELAWSRFDLLIHNKEADSFLSREEVRVYFISLFDRFKQLEPILVEAKTGNSVAASQFYRATQNLYMEMIDYV
TQHFRVASPLYKQQQKQAYDLLQAQYLLLGIFVITVALMTYFYKQESKFHKALALSDSLTGLANRSALFIDLQNKQESGT
TFSLLLLDLNGFKNINDTQGHQAGDIALTEVAERLKHMALDNFKVYRMGGDEFAVVINSTVKQDIEEAKRHIHVVFEKPI
FASDQASSLSTSLGAACYPQDTDNIDFLINLADKRMYKMKFQR
>Mature_363_residues
MKVQPNVPKQGPFSSRSKTLLLIFASTLLVANIVLLQATRALTKTYSDQQNQATWFLFHLSKELSELVGEARRLNESINN
FDSAELQYELAWSRFDLLIHNKEADSFLSREEVRVYFISLFDRFKQLEPILVEAKTGNSVAASQFYRATQNLYMEMIDYV
TQHFRVASPLYKQQQKQAYDLLQAQYLLLGIFVITVALMTYFYKQESKFHKALALSDSLTGLANRSALFIDLQNKQESGT
TFSLLLLDLNGFKNINDTQGHQAGDIALTEVAERLKHMALDNFKVYRMGGDEFAVVINSTVKQDIEEAKRHIHVVFEKPI
FASDQASSLSTSLGAACYPQDTDNIDFLINLADKRMYKMKFQR

Specific function: Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HAMP domain [H]

Homologues:

Organism=Escherichia coli, GI1788956, Length=155, Percent_Identity=36.7741935483871, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787056, Length=151, Percent_Identity=38.4105960264901, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI145693134, Length=171, Percent_Identity=29.8245614035088, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87081974, Length=137, Percent_Identity=30.6569343065693, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1787816, Length=266, Percent_Identity=25.9398496240602, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI87081977, Length=205, Percent_Identity=30.7317073170732, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1786584, Length=187, Percent_Identity=29.4117647058824, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87082007, Length=182, Percent_Identity=31.3186813186813, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787541, Length=152, Percent_Identity=30.9210526315789, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1787802, Length=150, Percent_Identity=32.6666666666667, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1787262, Length=160, Percent_Identity=27.5, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR003660 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 41510; Mature: 41510

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVQPNVPKQGPFSSRSKTLLLIFASTLLVANIVLLQATRALTKTYSDQQNQATWFLFHL
CCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHH
SKELSELVGEARRLNESINNFDSAELQYELAWSRFDLLIHNKEADSFLSREEVRVYFISL
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCEEEEEEECCCHHHHHHHHHHHHHHHHH
FDRFKQLEPILVEAKTGNSVAASQFYRATQNLYMEMIDYVTQHFRVASPLYKQQQKQAYD
HHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLQAQYLLLGIFVITVALMTYFYKQESKFHKALALSDSLTGLANRSALFIDLQNKQESGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCC
TFSLLLLDLNGFKNINDTQGHQAGDIALTEVAERLKHMALDNFKVYRMGGDEFAVVINST
EEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEECHH
VKQDIEEAKRHIHVVFEKPIFASDQASSLSTSLGAACYPQDTDNIDFLINLADKRMYKMK
HHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHCCCCCCCCCCCCCEEEEEEHHHHHHHHH
FQR
CCC
>Mature Secondary Structure
MKVQPNVPKQGPFSSRSKTLLLIFASTLLVANIVLLQATRALTKTYSDQQNQATWFLFHL
CCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHH
SKELSELVGEARRLNESINNFDSAELQYELAWSRFDLLIHNKEADSFLSREEVRVYFISL
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCEEEEEEECCCHHHHHHHHHHHHHHHHH
FDRFKQLEPILVEAKTGNSVAASQFYRATQNLYMEMIDYVTQHFRVASPLYKQQQKQAYD
HHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLQAQYLLLGIFVITVALMTYFYKQESKFHKALALSDSLTGLANRSALFIDLQNKQESGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCC
TFSLLLLDLNGFKNINDTQGHQAGDIALTEVAERLKHMALDNFKVYRMGGDEFAVVINST
EEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEECHH
VKQDIEEAKRHIHVVFEKPIFASDQASSLSTSLGAACYPQDTDNIDFLINLADKRMYKMK
HHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHCCCCCCCCCCCCCEEEEEEHHHHHHHHH
FQR
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 6357787; 7567469 [H]