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The map label for this gene is frmA [H]

Identifier: 156978120

GI number: 156978120

Start: 2021051

End: 2022199

Strand: Direct

Name: frmA [H]

Synonym: VIBHAR_06925

Alternate gene names: 156978120

Gene position: 2021051-2022199 (Clockwise)

Preceding gene: 156978115

Following gene: 156978121

Centisome position: 91.7

GC content: 45.95

Gene sequence:

>1149_bases
ATGGCACTTGAAATCAAACCAGGTCAAACTCACATTAAATCAAAAGCAATGGTTGCATGGGCTGCAGGTGAGCCGCTAAA
AATGGAAGAAGTTGATGTACAACTTCCAAAAGCTGGCGAAGTTCTTGTTCGCATCGTTGCTACTGGTGTTTGTCACACTG
ACGCATTCACTCTATCAGGTGACGATCCAGAAGGTATCTTCCCTTCAATCCTTGGCCACGAAGGCGGCGGTATCGTTGAA
ATGGTTGGTGAAGGCGTAACAAGCGTTGAAGTTGGCGACCACGTAATTCCGCTTTACACAGCAGAATGTGGTGAGTGTAA
GTTCTGTAAATCTGGCAAAACAAACCTATGTCAGGCAGTTCGTGAAACTCAAGGTAAAGGCCTAATGCCAGATGGCACAA
GCCGCTTCTCTGTAAACGGTGAGACAATCTTCCACTACATGGGTTGTTCTACTTTCTCTGAGTACACTGTACTTCCAGAA
ATCTCACTAGCAAAAGTAAACAAAGAAGCACCACTTGAAGAAGTTTGTCTTCTAGGTTGTGGCGTAACCACTGGTATGGG
TGCGGTACTAAACACAGCTAAAGTTGAAAAAGGCGACACTGTTGCTGTGTTCGGTCTAGGCGGTATCGGTCTTTCTGCAA
TCATCGGTGCTCGTATGGCTGGTGCAAGCAAGATCATCGGTGTAGACATCAACGAGAGCAAGTTCGAACTAGCAAAACAA
CTTGGCGCAACTGATTGCATCAACCCAATGAAATTCGACAAACCAATCCAAGACGTTATCGTTGAGATGACAGACGGTGG
TGTTGACTACTCATTCGAATGTATCGGTAACGTAAACGTGATGCGTCAAGCACTTGAGTGTTGTCACAAAGGTTGGGGTG
AGTCAGTAATCATTGGTGTTGCTGGTGCAGGTCAAGAGATCTCAACTCGTCCATTCCAACTAGTAACTGGTCGCGTATGG
CGTGGTTCTGCTTTCGGTGGCGTTAAAGGCCGCTCAGAGCTTCCAGAAATCGTAAACCGCTACATGGCTGGTGAGTTCGG
TCTACAAGAATTCATCACTCACACTATGGGTCTACAAGACGTGAACGAAGCGTTCGAATTGATGCACAAAGGTGAATCTA
TCCGTACTGTTCTTCACATGGATAAATAA

Upstream 100 bases:

>100_bases
ACCTAAAATGGGCACCAACAAAACGACGCAGCGCAGAGAGAAACACTCAACTACGCTTATGACGTCACTCTATATACTGA
GAATATAAAGGAAACATCCA

Downstream 100 bases:

>100_bases
TCCAAGATAACGGCTCACGTAATGTGACTGATATTTGAAAATTTGAAATAGGCTCTCTTCTTTCTTGAACACGTTTTCTT
TTGGGAAGAGAGCCATCCGC

Product: hypothetical protein

Products: NA

Alternate protein names: Alcohol dehydrogenase class-3; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH [H]

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MALEIKPGQTHIKSKAMVAWAAGEPLKMEEVDVQLPKAGEVLVRIVATGVCHTDAFTLSGDDPEGIFPSILGHEGGGIVE
MVGEGVTSVEVGDHVIPLYTAECGECKFCKSGKTNLCQAVRETQGKGLMPDGTSRFSVNGETIFHYMGCSTFSEYTVLPE
ISLAKVNKEAPLEEVCLLGCGVTTGMGAVLNTAKVEKGDTVAVFGLGGIGLSAIIGARMAGASKIIGVDINESKFELAKQ
LGATDCINPMKFDKPIQDVIVEMTDGGVDYSFECIGNVNVMRQALECCHKGWGESVIIGVAGAGQEISTRPFQLVTGRVW
RGSAFGGVKGRSELPEIVNRYMAGEFGLQEFITHTMGLQDVNEAFELMHKGESIRTVLHMDK

Sequences:

>Translated_382_residues
MALEIKPGQTHIKSKAMVAWAAGEPLKMEEVDVQLPKAGEVLVRIVATGVCHTDAFTLSGDDPEGIFPSILGHEGGGIVE
MVGEGVTSVEVGDHVIPLYTAECGECKFCKSGKTNLCQAVRETQGKGLMPDGTSRFSVNGETIFHYMGCSTFSEYTVLPE
ISLAKVNKEAPLEEVCLLGCGVTTGMGAVLNTAKVEKGDTVAVFGLGGIGLSAIIGARMAGASKIIGVDINESKFELAKQ
LGATDCINPMKFDKPIQDVIVEMTDGGVDYSFECIGNVNVMRQALECCHKGWGESVIIGVAGAGQEISTRPFQLVTGRVW
RGSAFGGVKGRSELPEIVNRYMAGEFGLQEFITHTMGLQDVNEAFELMHKGESIRTVLHMDK
>Mature_381_residues
ALEIKPGQTHIKSKAMVAWAAGEPLKMEEVDVQLPKAGEVLVRIVATGVCHTDAFTLSGDDPEGIFPSILGHEGGGIVEM
VGEGVTSVEVGDHVIPLYTAECGECKFCKSGKTNLCQAVRETQGKGLMPDGTSRFSVNGETIFHYMGCSTFSEYTVLPEI
SLAKVNKEAPLEEVCLLGCGVTTGMGAVLNTAKVEKGDTVAVFGLGGIGLSAIIGARMAGASKIIGVDINESKFELAKQL
GATDCINPMKFDKPIQDVIVEMTDGGVDYSFECIGNVNVMRQALECCHKGWGESVIIGVAGAGQEISTRPFQLVTGRVWR
GSAFGGVKGRSELPEIVNRYMAGEFGLQEFITHTMGLQDVNEAFELMHKGESIRTVLHMDK

Specific function: Has High Formaldehyde Dehydrogenase Activity In The Presence Of Glutathione And Catalyzes The Oxidation Of Normal Alcohols In A Reaction That Is Not Gsh-Dependent. In Addition, Hemithiolacetals Other Than Those Formed From Gsh, Including Omega-Thiol Fatty

COG id: COG1062

COG function: function code C; Zn-dependent alcohol dehydrogenases, class III

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily [H]

Homologues:

Organism=Homo sapiens, GI71565154, Length=369, Percent_Identity=65.5826558265583, Blast_Score=508, Evalue=1e-144,
Organism=Homo sapiens, GI34577061, Length=367, Percent_Identity=51.4986376021798, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI4501929, Length=367, Percent_Identity=50.9536784741144, Blast_Score=390, Evalue=1e-108,
Organism=Homo sapiens, GI4501933, Length=367, Percent_Identity=50.1362397820163, Blast_Score=389, Evalue=1e-108,
Organism=Homo sapiens, GI71565152, Length=371, Percent_Identity=50.1347708894879, Blast_Score=385, Evalue=1e-107,
Organism=Homo sapiens, GI156523966, Length=369, Percent_Identity=47.9674796747967, Blast_Score=374, Evalue=1e-104,
Organism=Homo sapiens, GI4501939, Length=361, Percent_Identity=48.1994459833795, Blast_Score=367, Evalue=1e-101,
Organism=Homo sapiens, GI262073058, Length=367, Percent_Identity=47.4114441416894, Blast_Score=365, Evalue=1e-101,
Organism=Homo sapiens, GI71743840, Length=368, Percent_Identity=47.554347826087, Blast_Score=365, Evalue=1e-101,
Organism=Escherichia coli, GI1786552, Length=367, Percent_Identity=70.0272479564033, Blast_Score=532, Evalue=1e-152,
Organism=Escherichia coli, GI1790045, Length=373, Percent_Identity=28.1501340482574, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI87082125, Length=366, Percent_Identity=25.4098360655738, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1787863, Length=350, Percent_Identity=25.4285714285714, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788073, Length=364, Percent_Identity=27.1978021978022, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI1786825, Length=260, Percent_Identity=29.2307692307692, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI87081918, Length=221, Percent_Identity=33.4841628959276, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1788075, Length=369, Percent_Identity=26.0162601626016, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI226510992, Length=186, Percent_Identity=25.2688172043011, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786518, Length=372, Percent_Identity=26.6129032258064, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI25146526, Length=375, Percent_Identity=62.1333333333333, Blast_Score=493, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI71997431, Length=377, Percent_Identity=61.8037135278515, Blast_Score=488, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI17565904, Length=377, Percent_Identity=57.5596816976127, Blast_Score=453, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI17562584, Length=367, Percent_Identity=28.0653950953678, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17562876, Length=361, Percent_Identity=26.5927977839335, Blast_Score=95, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17562878, Length=361, Percent_Identity=25.4847645429363, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71988145, Length=362, Percent_Identity=26.7955801104972, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17562582, Length=363, Percent_Identity=27.5482093663912, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6320033, Length=375, Percent_Identity=61.0666666666667, Blast_Score=486, Evalue=1e-138,
Organism=Saccharomyces cerevisiae, GI6319621, Length=378, Percent_Identity=30.6878306878307, Blast_Score=109, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6323729, Length=365, Percent_Identity=28.2191780821918, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324486, Length=375, Percent_Identity=26.9333333333333, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6323961, Length=364, Percent_Identity=27.4725274725275, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6323099, Length=375, Percent_Identity=28.2666666666667, Blast_Score=96, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6322619, Length=356, Percent_Identity=28.0898876404494, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6319955, Length=356, Percent_Identity=28.0898876404494, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6319258, Length=226, Percent_Identity=30.5309734513274, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6319257, Length=277, Percent_Identity=25.2707581227437, Blast_Score=66, Evalue=9e-12,
Organism=Drosophila melanogaster, GI17737895, Length=369, Percent_Identity=63.9566395663957, Blast_Score=499, Evalue=1e-141,
Organism=Drosophila melanogaster, GI17137530, Length=365, Percent_Identity=26.3013698630137, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI17737897, Length=374, Percent_Identity=24.331550802139, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014183
- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.284; =1.1.1.1 [H]

Molecular weight: Translated: 40698; Mature: 40567

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALEIKPGQTHIKSKAMVAWAAGEPLKMEEVDVQLPKAGEVLVRIVATGVCHTDAFTLSG
CEEECCCCCHHHCCCEEEEEECCCCCEECCCCEECCCHHHHHHHHHHHCCCCCCEEEECC
DDPEGIFPSILGHEGGGIVEMVGEGVTSVEVGDHVIPLYTAECGECKFCKSGKTNLCQAV
CCCCCCCHHHHCCCCCCCCEECCCCCCEEECCCEEEEEEECCCCCEECCCCCCHHHHHHH
RETQGKGLMPDGTSRFSVNGETIFHYMGCSTFSEYTVLPEISLAKVNKEAPLEEVCLLGC
HHHCCCCCCCCCCCEEEECCCEEEEEECCCCCCCEEECCCCHHHHCCCCCCHHHHHHHHC
GVTTGMGAVLNTAKVEKGDTVAVFGLGGIGLSAIIGARMAGASKIIGVDINESKFELAKQ
CHHHCHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHH
LGATDCINPMKFDKPIQDVIVEMTDGGVDYSFECIGNVNVMRQALECCHKGWGESVIIGV
CCCCCCCCCCCCCCCHHHHHEECCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEE
AGAGQEISTRPFQLVTGRVWRGSAFGGVKGRSELPEIVNRYMAGEFGLQEFITHTMGLQD
ECCCCCCCCCCEEEEECEEECCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCHHH
VNEAFELMHKGESIRTVLHMDK
HHHHHHHHHCCCCEEEEEECCC
>Mature Secondary Structure 
ALEIKPGQTHIKSKAMVAWAAGEPLKMEEVDVQLPKAGEVLVRIVATGVCHTDAFTLSG
EEECCCCCHHHCCCEEEEEECCCCCEECCCCEECCCHHHHHHHHHHHCCCCCCEEEECC
DDPEGIFPSILGHEGGGIVEMVGEGVTSVEVGDHVIPLYTAECGECKFCKSGKTNLCQAV
CCCCCCCHHHHCCCCCCCCEECCCCCCEEECCCEEEEEEECCCCCEECCCCCCHHHHHHH
RETQGKGLMPDGTSRFSVNGETIFHYMGCSTFSEYTVLPEISLAKVNKEAPLEEVCLLGC
HHHCCCCCCCCCCCEEEECCCEEEEEECCCCCCCEEECCCCHHHHCCCCCCHHHHHHHHC
GVTTGMGAVLNTAKVEKGDTVAVFGLGGIGLSAIIGARMAGASKIIGVDINESKFELAKQ
CHHHCHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHH
LGATDCINPMKFDKPIQDVIVEMTDGGVDYSFECIGNVNVMRQALECCHKGWGESVIIGV
CCCCCCCCCCCCCCCHHHHHEECCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEE
AGAGQEISTRPFQLVTGRVWRGSAFGGVKGRSELPEIVNRYMAGEFGLQEFITHTMGLQD
ECCCCCCCCCCEEEEECEEECCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCHHH
VNEAFELMHKGESIRTVLHMDK
HHHHHHHHHCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]