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The map label for this gene is emrB [H]

Identifier: 156978098

GI number: 156978098

Start: 2000550

End: 2002037

Strand: Reverse

Name: emrB [H]

Synonym: VIBHAR_06902

Alternate gene names: 156978098

Gene position: 2002037-2000550 (Counterclockwise)

Preceding gene: 156978099

Following gene: 156978096

Centisome position: 90.84

GC content: 49.53

Gene sequence:

>1488_bases
ATGAGTAACGAGATTACGGCCGACAAACGGGGAATAGCCACCGTCGCCGTGATGCTTTCGGCCATCATGGTGCTGATAGA
CATGACCGTTGCTAACGTGTCGCTTTCACACATGATGGGCGCATTGGGTGCAACGGCAGACCAAATCACTTGGGTGCTAA
CTGGCTACAGCATGGCTGAGGCGATCTTCATTCCGATGACCAGTTTCTGGGTATCGAGATTTGGTGAGCGTAAAGTCATG
CTGGTGGCGGTCATCGGCTTTGTGATTGCCAGTGCTTTCTGTGGACAAGCGGGTTCACTCGAAGAGATGGTAGTGTTCCG
TATTATCCAAGGTGCGTTTGGTGCTTCGGTTATTCCACTAGCGCAATCGACACTGGTTCAGATTTACCCTTCCGAACAAA
AAGGCAAGGCGATGGCGATCTTCTCCATCGGTATCTTGCTTGGTCCGATTTTAGGCCCGGTAGTGGGCGGTGTGATTACT
GATAACATCAACTGGCGTTGGATCTTCTACGTCAACTTGCCATTTGGCTTGGTGTGTACTGCGCTGATTTATCGCTACAT
CCACATTAGCAACAAATCCAAACCAAACTTCGACTGGTGGATCATTGGCTATATGGCCCTCGGGGTCGGTGCGTTGCAGT
TCGTACTCGACAAGGGTAACGACGAAGACTGGTTTAACTCCCGCATTATTCAAACCGCAGTGCTGCTGGCGATCATGGGC
TTAATCATGTGGATCTACCGCAGTTGGAAGACCAAGAGCCCCATTGCGCCTCTGTGGCTCCTTAAGGACAAAAACCTTAT
GGTCTCGTCCTTAATGATGGCGGTGGTGTCGATGGCCATGTTTGGCTTGACCACTCAGCAACCGATGCTGCTCGAAAGCT
TATTGAACTACCCAGTATCCACCACGGGTATGTTAATGGCACCACGGGGGCTGGCGTCCGCTTGTATGCTGATATTGGTG
ATTGTGATTAACCCACAATTCGATCCAAGGTTGAAGATTGTCTTTGGCTTAACCTGTATCGGTATTGGTAGTTATCTCAT
GACCTTGTACTCCATGGAAATCGATACCTTCTGGATCGTGATGCCAAGTATGATTCAAGGTATGGGCTTAGGTTTGACCT
TCTCGACCTTATCGACACTGGCGTACATGACATTGCCAAAAGAGCAGTCGGTGGCAGGTGCGAGTATTTTTAACTTGTTC
CGCACCATTGGTAGTTCGTTTGGTATCTCGATTGCGACTACGTACCAATACCGTGACAGCCAACAGCAATGGCACGCTTT
GGGAGAAGGCTTTAACCCTTATAACCCAGTGTTGCATGACTGGGCAGCGAAACAGGGACTATCGATTACCGATCCTGCGG
CGTTGGAGCAATACCAGAACATGCTCCATCAACAATCGCAAATGGTAGCCTTTGTGCACACCTTCCAGTTGGTCGGGGTG
ATGTTCGTTATCATGATGCCGATGCTGATATTTATCCGCTCAAGGTAA

Upstream 100 bases:

>100_bases
GTTTCCCTGTGCGTTTGACGCTTGAAGTACCTGAAGGTGCGCCACAATTACGTGTGGGTGCAAGCTCCGAAGTGACTGTT
GATCTGAAAAGTAACGCACA

Downstream 100 bases:

>100_bases
ACCTCTAACGCCAAACAAAAGCGCACTTAACTCAGCCAGTTAAGGGCGCTTTTTTATGTCTGACGTTTCTATCGGTTTTC
GAGCAGTTAACCGAAGGTCA

Product: hypothetical protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MSNEITADKRGIATVAVMLSAIMVLIDMTVANVSLSHMMGALGATADQITWVLTGYSMAEAIFIPMTSFWVSRFGERKVM
LVAVIGFVIASAFCGQAGSLEEMVVFRIIQGAFGASVIPLAQSTLVQIYPSEQKGKAMAIFSIGILLGPILGPVVGGVIT
DNINWRWIFYVNLPFGLVCTALIYRYIHISNKSKPNFDWWIIGYMALGVGALQFVLDKGNDEDWFNSRIIQTAVLLAIMG
LIMWIYRSWKTKSPIAPLWLLKDKNLMVSSLMMAVVSMAMFGLTTQQPMLLESLLNYPVSTTGMLMAPRGLASACMLILV
IVINPQFDPRLKIVFGLTCIGIGSYLMTLYSMEIDTFWIVMPSMIQGMGLGLTFSTLSTLAYMTLPKEQSVAGASIFNLF
RTIGSSFGISIATTYQYRDSQQQWHALGEGFNPYNPVLHDWAAKQGLSITDPAALEQYQNMLHQQSQMVAFVHTFQLVGV
MFVIMMPMLIFIRSR

Sequences:

>Translated_495_residues
MSNEITADKRGIATVAVMLSAIMVLIDMTVANVSLSHMMGALGATADQITWVLTGYSMAEAIFIPMTSFWVSRFGERKVM
LVAVIGFVIASAFCGQAGSLEEMVVFRIIQGAFGASVIPLAQSTLVQIYPSEQKGKAMAIFSIGILLGPILGPVVGGVIT
DNINWRWIFYVNLPFGLVCTALIYRYIHISNKSKPNFDWWIIGYMALGVGALQFVLDKGNDEDWFNSRIIQTAVLLAIMG
LIMWIYRSWKTKSPIAPLWLLKDKNLMVSSLMMAVVSMAMFGLTTQQPMLLESLLNYPVSTTGMLMAPRGLASACMLILV
IVINPQFDPRLKIVFGLTCIGIGSYLMTLYSMEIDTFWIVMPSMIQGMGLGLTFSTLSTLAYMTLPKEQSVAGASIFNLF
RTIGSSFGISIATTYQYRDSQQQWHALGEGFNPYNPVLHDWAAKQGLSITDPAALEQYQNMLHQQSQMVAFVHTFQLVGV
MFVIMMPMLIFIRSR
>Mature_494_residues
SNEITADKRGIATVAVMLSAIMVLIDMTVANVSLSHMMGALGATADQITWVLTGYSMAEAIFIPMTSFWVSRFGERKVML
VAVIGFVIASAFCGQAGSLEEMVVFRIIQGAFGASVIPLAQSTLVQIYPSEQKGKAMAIFSIGILLGPILGPVVGGVITD
NINWRWIFYVNLPFGLVCTALIYRYIHISNKSKPNFDWWIIGYMALGVGALQFVLDKGNDEDWFNSRIIQTAVLLAIMGL
IMWIYRSWKTKSPIAPLWLLKDKNLMVSSLMMAVVSMAMFGLTTQQPMLLESLLNYPVSTTGMLMAPRGLASACMLILVI
VINPQFDPRLKIVFGLTCIGIGSYLMTLYSMEIDTFWIVMPSMIQGMGLGLTFSTLSTLAYMTLPKEQSVAGASIFNLFR
TIGSSFGISIATTYQYRDSQQQWHALGEGFNPYNPVLHDWAAKQGLSITDPAALEQYQNMLHQQSQMVAFVHTFQLVGVM
FVIMMPMLIFIRSR

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=488, Percent_Identity=30.1229508196721, Blast_Score=236, Evalue=3e-63,
Organism=Escherichia coli, GI1788710, Length=488, Percent_Identity=29.5081967213115, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI1790195, Length=417, Percent_Identity=24.9400479616307, Blast_Score=146, Evalue=4e-36,
Organism=Escherichia coli, GI87081983, Length=417, Percent_Identity=26.378896882494, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1788392, Length=392, Percent_Identity=25, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321663, Length=133, Percent_Identity=33.8345864661654, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6325455, Length=129, Percent_Identity=27.1317829457364, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6322958, Length=163, Percent_Identity=26.3803680981595, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323000, Length=153, Percent_Identity=27.4509803921569, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319770, Length=383, Percent_Identity=22.976501305483, Blast_Score=68, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 54760; Mature: 54629

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
7.1 %Met     (Translated Protein)
7.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
6.9 %Met     (Mature Protein)
7.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNEITADKRGIATVAVMLSAIMVLIDMTVANVSLSHMMGALGATADQITWVLTGYSMAE
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEEEECHHHHH
AIFIPMTSFWVSRFGERKVMLVAVIGFVIASAFCGQAGSLEEMVVFRIIQGAFGASVIPL
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHH
AQSTLVQIYPSEQKGKAMAIFSIGILLGPILGPVVGGVITDNINWRWIFYVNLPFGLVCT
HHCCEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHCCCEECCCCEEEEEEEECCHHHHHH
ALIYRYIHISNKSKPNFDWWIIGYMALGVGALQFVLDKGNDEDWFNSRIIQTAVLLAIMG
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
LIMWIYRSWKTKSPIAPLWLLKDKNLMVSSLMMAVVSMAMFGLTTQQPMLLESLLNYPVS
HHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCC
TTGMLMAPRGLASACMLILVIVINPQFDPRLKIVFGLTCIGIGSYLMTLYSMEIDTFWIV
CCCEEECCCCHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHEEE
MPSMIQGMGLGLTFSTLSTLAYMTLPKEQSVAGASIFNLFRTIGSSFGISIATTYQYRDS
HHHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCH
QQQWHALGEGFNPYNPVLHDWAAKQGLSITDPAALEQYQNMLHQQSQMVAFVHTFQLVGV
HHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MFVIMMPMLIFIRSR
HHHHHHHHHHHHCCC
>Mature Secondary Structure 
SNEITADKRGIATVAVMLSAIMVLIDMTVANVSLSHMMGALGATADQITWVLTGYSMAE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEEEECHHHHH
AIFIPMTSFWVSRFGERKVMLVAVIGFVIASAFCGQAGSLEEMVVFRIIQGAFGASVIPL
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHH
AQSTLVQIYPSEQKGKAMAIFSIGILLGPILGPVVGGVITDNINWRWIFYVNLPFGLVCT
HHCCEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHCCCEECCCCEEEEEEEECCHHHHHH
ALIYRYIHISNKSKPNFDWWIIGYMALGVGALQFVLDKGNDEDWFNSRIIQTAVLLAIMG
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
LIMWIYRSWKTKSPIAPLWLLKDKNLMVSSLMMAVVSMAMFGLTTQQPMLLESLLNYPVS
HHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCC
TTGMLMAPRGLASACMLILVIVINPQFDPRLKIVFGLTCIGIGSYLMTLYSMEIDTFWIV
CCCEEECCCCHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHEEE
MPSMIQGMGLGLTFSTLSTLAYMTLPKEQSVAGASIFNLFRTIGSSFGISIATTYQYRDS
HHHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCH
QQQWHALGEGFNPYNPVLHDWAAKQGLSITDPAALEQYQNMLHQQSQMVAFVHTFQLVGV
HHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MFVIMMPMLIFIRSR
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]