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The map label for this gene is pmbA [H]

Identifier: 156978032

GI number: 156978032

Start: 1946680

End: 1948023

Strand: Reverse

Name: pmbA [H]

Synonym: VIBHAR_06836

Alternate gene names: 156978032

Gene position: 1948023-1946680 (Counterclockwise)

Preceding gene: 156978033

Following gene: 156978030

Centisome position: 88.39

GC content: 47.99

Gene sequence:

>1344_bases
ATGAGCCAAGAAAAACAACTTCTTAATGCGGTTGATTACGTCCTATCAGAAGCGAAGCGCCAAGGCGCAGAAGCGGACGT
CATTGTAAACCGCAACAGCAGCTTTTCTCTGAAAGCGAACCAAGGGCAGTTGGATGAGTACAAAGTAAGCTCAAGCCAAG
TTCTGGGTGTTCGTGTTGTTAAAGATGCGCGTGTCGCAACGAGCTACTCTGAATCGTTAGAACAACCGAGCTTAGATTTG
ATGCTGACTAATGCACTGCAAAGCGCTCGTTTTTCCAAGCAAGACGAACACCAGACCATTAGTTGCGTGAACAGCCAAAT
CACGACTGACGTGGCTGAGATTGCTCAAGAAGACACAACTTCGGTTGATGAAAAGATTGAAATGTCTCTCGCTCTTGAGC
GAGGCGTAGTCGCTCTGCCTCACGCTTCTAGCGCACCTTACAATGGTTATAGTGATGGCGAAACGCAATTAATCATCGCC
AATACACAAGGCTCATTGTGCCATCACTTCGAACGCTCGTTTACTTGTTACGCGTACACCTTGTTTGAAAAAGACGGTAA
ACAGTCGATGGCAGGGCAAATGTCACTTGGTCGTCGTTTTGATGAGTTGAACCCTGCTTACTGTATCGAAGGTGGTTACA
ACCTTGCTCGCGATCTTCTTGACGGCGCTCCAGTTGCGACGGGTAACTACCCAGCAATCTTCCATATCAATGCGCTAGCA
AGTTTGTTCGGTGCTTTTGGCAGTGCTTTCTCTGGCGTAAGTGCGATGAAAGGCATTACACCATTGGGTGACAAGCTTGG
CCAACGTATTGCGAGTGATTTGATTACCTTTACCGACGCGGCTTACATGCCAAATGGTATGGCAATTTCGGGCTTTGATA
GTGAGGGTTTTACTACTCAAGACACCGTCATGATTGCTAATGGTGAATTGAAAACTTTGCTACACAACAGCCAGACGGCG
AGTTACTTGGGCGCAGTATCTACCGCAAGTGCTGCTCGTGGTGCAAAATCGAGCTTAGACGTATCAGCGAATCACAAAGT
GATTGCGACAGGCAACAGCAGTGCGTCAGAAGTGAAAGCGGGTGAGTACCTAGAGTTAGTTGAACTGCAGGGTGTTCACT
CTGGCGCGGATGCAGTGAGTGGTGATTTCTCGTTTGGCGCGAGTGGCTTCTTGTGCCGTGATGGAGAGCGCGTTCAACCT
GTTCGTGGTATTACGGTTGCGGGTAACTTCTACAAGATGCTGCAAGAAGTTGAGGCTGTGGGTGATACTCAGCTAATTAA
CGATAGCCGAACTTTCTTCTCGCCAGATGTTCGTTTTGCTCGTTTGAGCATTGGTGGTAAATAA

Upstream 100 bases:

>100_bases
TTGCGCCGGGCATGTGTGGTTCCGTGAGTGGTTCAGTGCCAACAACAGTCGGTCAGCCAACGCTGAAAGTCGATAACATT
CTGGTAGGAGGCGGTAACTA

Downstream 100 bases:

>100_bases
CCCCAGCATTCCTATAGAAACAAAAAAGAGGACTAAACTGAGTCCTCTTTCTTTTATAGAGTGATTAACTCGCGAGAATT
AAAATTCGCGAATGTCCAAC

Product: peptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MSQEKQLLNAVDYVLSEAKRQGAEADVIVNRNSSFSLKANQGQLDEYKVSSSQVLGVRVVKDARVATSYSESLEQPSLDL
MLTNALQSARFSKQDEHQTISCVNSQITTDVAEIAQEDTTSVDEKIEMSLALERGVVALPHASSAPYNGYSDGETQLIIA
NTQGSLCHHFERSFTCYAYTLFEKDGKQSMAGQMSLGRRFDELNPAYCIEGGYNLARDLLDGAPVATGNYPAIFHINALA
SLFGAFGSAFSGVSAMKGITPLGDKLGQRIASDLITFTDAAYMPNGMAISGFDSEGFTTQDTVMIANGELKTLLHNSQTA
SYLGAVSTASAARGAKSSLDVSANHKVIATGNSSASEVKAGEYLELVELQGVHSGADAVSGDFSFGASGFLCRDGERVQP
VRGITVAGNFYKMLQEVEAVGDTQLINDSRTFFSPDVRFARLSIGGK

Sequences:

>Translated_447_residues
MSQEKQLLNAVDYVLSEAKRQGAEADVIVNRNSSFSLKANQGQLDEYKVSSSQVLGVRVVKDARVATSYSESLEQPSLDL
MLTNALQSARFSKQDEHQTISCVNSQITTDVAEIAQEDTTSVDEKIEMSLALERGVVALPHASSAPYNGYSDGETQLIIA
NTQGSLCHHFERSFTCYAYTLFEKDGKQSMAGQMSLGRRFDELNPAYCIEGGYNLARDLLDGAPVATGNYPAIFHINALA
SLFGAFGSAFSGVSAMKGITPLGDKLGQRIASDLITFTDAAYMPNGMAISGFDSEGFTTQDTVMIANGELKTLLHNSQTA
SYLGAVSTASAARGAKSSLDVSANHKVIATGNSSASEVKAGEYLELVELQGVHSGADAVSGDFSFGASGFLCRDGERVQP
VRGITVAGNFYKMLQEVEAVGDTQLINDSRTFFSPDVRFARLSIGGK
>Mature_446_residues
SQEKQLLNAVDYVLSEAKRQGAEADVIVNRNSSFSLKANQGQLDEYKVSSSQVLGVRVVKDARVATSYSESLEQPSLDLM
LTNALQSARFSKQDEHQTISCVNSQITTDVAEIAQEDTTSVDEKIEMSLALERGVVALPHASSAPYNGYSDGETQLIIAN
TQGSLCHHFERSFTCYAYTLFEKDGKQSMAGQMSLGRRFDELNPAYCIEGGYNLARDLLDGAPVATGNYPAIFHINALAS
LFGAFGSAFSGVSAMKGITPLGDKLGQRIASDLITFTDAAYMPNGMAISGFDSEGFTTQDTVMIANGELKTLLHNSQTAS
YLGAVSTASAARGAKSSLDVSANHKVIATGNSSASEVKAGEYLELVELQGVHSGADAVSGDFSFGASGFLCRDGERVQPV
RGITVAGNFYKMLQEVEAVGDTQLINDSRTFFSPDVRFARLSIGGK

Specific function: May Facilitate The Secretion Of The Peptide Antibiotic Microcin B17 (Mccb17) By Completing Its Maturation. Suppresses The Inhibitory Activity Of The Carbon Storage Regulator (Csra). [C]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=431, Percent_Identity=25.9860788863109, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1789640, Length=214, Percent_Identity=26.6355140186916, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 47736; Mature: 47604

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQEKQLLNAVDYVLSEAKRQGAEADVIVNRNSSFSLKANQGQLDEYKVSSSQVLGVRVV
CCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEECCCCCCCCEECCCCCEEEEEEE
KDARVATSYSESLEQPSLDLMLTNALQSARFSKQDEHQTISCVNSQITTDVAEIAQEDTT
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
SVDEKIEMSLALERGVVALPHASSAPYNGYSDGETQLIIANTQGSLCHHFERSFTCYAYT
CHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHCCCEEEEEE
LFEKDGKQSMAGQMSLGRRFDELNPAYCIEGGYNLARDLLDGAPVATGNYPAIFHINALA
EECCCCCHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHCCCCCCCCCCCEEEEHHHHH
SLFGAFGSAFSGVSAMKGITPLGDKLGQRIASDLITFTDAAYMPNGMAISGFDSEGFTTQ
HHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCC
DTVMIANGELKTLLHNSQTASYLGAVSTASAARGAKSSLDVSANHKVIATGNSSASEVKA
CEEEEECCCEEEHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCHHHCCC
GEYLELVELQGVHSGADAVSGDFSFGASGFLCRDGERVQPVRGITVAGNFYKMLQEVEAV
CCCEEEHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCEEECHHHHHHHHHHHHC
GDTQLINDSRTFFSPDVRFARLSIGGK
CCCEEECCCCCEECCCCEEEEEECCCC
>Mature Secondary Structure 
SQEKQLLNAVDYVLSEAKRQGAEADVIVNRNSSFSLKANQGQLDEYKVSSSQVLGVRVV
CHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEECCCCCCCCEECCCCCEEEEEEE
KDARVATSYSESLEQPSLDLMLTNALQSARFSKQDEHQTISCVNSQITTDVAEIAQEDTT
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
SVDEKIEMSLALERGVVALPHASSAPYNGYSDGETQLIIANTQGSLCHHFERSFTCYAYT
CHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHCCCEEEEEE
LFEKDGKQSMAGQMSLGRRFDELNPAYCIEGGYNLARDLLDGAPVATGNYPAIFHINALA
EECCCCCHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHCCCCCCCCCCCEEEEHHHHH
SLFGAFGSAFSGVSAMKGITPLGDKLGQRIASDLITFTDAAYMPNGMAISGFDSEGFTTQ
HHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCC
DTVMIANGELKTLLHNSQTASYLGAVSTASAARGAKSSLDVSANHKVIATGNSSASEVKA
CEEEEECCCEEEHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCHHHCCC
GEYLELVELQGVHSGADAVSGDFSFGASGFLCRDGERVQPVRGITVAGNFYKMLQEVEAV
CCCEEEHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCEEECHHHHHHHHHHHHC
GDTQLINDSRTFFSPDVRFARLSIGGK
CCCEEECCCCCEECCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]