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The map label for this gene is dmlR [H]

Identifier: 156977860

GI number: 156977860

Start: 1789356

End: 1790285

Strand: Direct

Name: dmlR [H]

Synonym: VIBHAR_06651

Alternate gene names: 156977860

Gene position: 1789356-1790285 (Clockwise)

Preceding gene: 156977858

Following gene: 156977863

Centisome position: 81.19

GC content: 47.74

Gene sequence:

>930_bases
ATGAAAGCCCTCAACGATCTGAATATCTTTGTCGAAACCGCACGCCAAGGCAGTTTTTCGAAAGCCGCCAACAGCATGGA
CATGACACCTGCAGCCATCAGTGCATCGATCAAACGTCTTGAAGGGCAGATTGGCTTTCCGCTGTTTGTACGCTCGACTC
GCAGCCTGCGCCTAACCTCCGAAGGTGAACTGTTCCTAGATAAAACCACCCAAGCATTGGCAACATTGCAAGAAGGTTTG
GATCAGATCTCAAGCGCGCGTGGTGAGCTATCAGGTCAATTGCACATCACGGCACCATCTGACTTTGGTCGTAACATGCT
GCTTGATTGGGTCGATGAGTTTATCGAGCTCTACCCGAACGTCTCCATCAAGTTAGAGCTGTCTGACAGCTTGACGGATA
TGTACACCAAACCCGTCGATATTGCGATTCGATACGGTGAGCCCGCAGATTCTAACTTAGTTGTGCTGGCATTATGCGGT
GCGAACGAACGGATCTTATGTGCCTCCCCAGAATACGTTGCAAGCAATCCAGAATTGACCAAACCAGAAGATCTCCACCA
GCACAATTGCTTGTGTTTCATGGTAGCGGACTCGGTTTACAACAAATGGACACTGACTCGCGAAGGTGAAACAGAACAGG
TTGTCGTAAGTGGTAAACTACTCAGTAACGACAGTGACTTATCCCATCGCTTAGCAATTAAAGGCAAAGGCATTGCCAAT
AAATCACTGATGGACATCAGCCAAGATTTGATTGAAGGAAGATTGGTACGCGTACTGCCTGAGTGGGATTGTGGTTCCGT
ACCGCTTTACATGGTGTGCGCTGATCGTCGATTGCTAACACCAACGATTCGTACTTTCCGAGATTTTATCCAAGAGAAAT
GTTGCCAGCAAAGGAAGTCTGTACTGACAACATTCTGTTCAGAGCGTTAA

Upstream 100 bases:

>100_bases
ATCTAACTCAAACTCGCCGTGCAACCTAATGTTTTAGCCTTGAATTGCCCTGCGACTCTCCCCTATAGTAGCTCAAACTT
CTTAAAAGCCCCTTTTCATT

Downstream 100 bases:

>100_bases
CCAACAAACGTCATCGGTCGGATATGGGTGTACTCAAGTAGGCCGTTCTTTTCTAATGTAGAAAAACATTGGGTAATGTG
AGGGCTTTGTAAGTGCTTGT

Product: transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MKALNDLNIFVETARQGSFSKAANSMDMTPAAISASIKRLEGQIGFPLFVRSTRSLRLTSEGELFLDKTTQALATLQEGL
DQISSARGELSGQLHITAPSDFGRNMLLDWVDEFIELYPNVSIKLELSDSLTDMYTKPVDIAIRYGEPADSNLVVLALCG
ANERILCASPEYVASNPELTKPEDLHQHNCLCFMVADSVYNKWTLTREGETEQVVVSGKLLSNDSDLSHRLAIKGKGIAN
KSLMDISQDLIEGRLVRVLPEWDCGSVPLYMVCADRRLLTPTIRTFRDFIQEKCCQQRKSVLTTFCSER

Sequences:

>Translated_309_residues
MKALNDLNIFVETARQGSFSKAANSMDMTPAAISASIKRLEGQIGFPLFVRSTRSLRLTSEGELFLDKTTQALATLQEGL
DQISSARGELSGQLHITAPSDFGRNMLLDWVDEFIELYPNVSIKLELSDSLTDMYTKPVDIAIRYGEPADSNLVVLALCG
ANERILCASPEYVASNPELTKPEDLHQHNCLCFMVADSVYNKWTLTREGETEQVVVSGKLLSNDSDLSHRLAIKGKGIAN
KSLMDISQDLIEGRLVRVLPEWDCGSVPLYMVCADRRLLTPTIRTFRDFIQEKCCQQRKSVLTTFCSER
>Mature_309_residues
MKALNDLNIFVETARQGSFSKAANSMDMTPAAISASIKRLEGQIGFPLFVRSTRSLRLTSEGELFLDKTTQALATLQEGL
DQISSARGELSGQLHITAPSDFGRNMLLDWVDEFIELYPNVSIKLELSDSLTDMYTKPVDIAIRYGEPADSNLVVLALCG
ANERILCASPEYVASNPELTKPEDLHQHNCLCFMVADSVYNKWTLTREGETEQVVVSGKLLSNDSDLSHRLAIKGKGIAN
KSLMDISQDLIEGRLVRVLPEWDCGSVPLYMVCADRRLLTPTIRTFRDFIQEKCCQQRKSVLTTFCSER

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=294, Percent_Identity=29.5918367346939, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1789440, Length=294, Percent_Identity=30.6122448979592, Blast_Score=144, Evalue=8e-36,
Organism=Escherichia coli, GI1786401, Length=301, Percent_Identity=32.2259136212625, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1787128, Length=297, Percent_Identity=27.2727272727273, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1787589, Length=293, Percent_Identity=25.938566552901, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1789639, Length=277, Percent_Identity=27.4368231046931, Blast_Score=110, Evalue=8e-26,
Organism=Escherichia coli, GI145693193, Length=293, Percent_Identity=27.3037542662116, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1789173, Length=299, Percent_Identity=30.1003344481605, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1786448, Length=253, Percent_Identity=30.4347826086957, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1788706, Length=261, Percent_Identity=26.0536398467433, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787601, Length=169, Percent_Identity=26.6272189349112, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI157672245, Length=145, Percent_Identity=30.3448275862069, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790208, Length=151, Percent_Identity=33.7748344370861, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34435; Mature: 34435

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKALNDLNIFVETARQGSFSKAANSMDMTPAAISASIKRLEGQIGFPLFVRSTRSLRLTS
CCCCHHHHHEEECCCCCCHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCEEEEEC
EGELFLDKTTQALATLQEGLDQISSARGELSGQLHITAPSDFGRNMLLDWVDEFIELYPN
CCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCC
VSIKLELSDSLTDMYTKPVDIAIRYGEPADSNLVVLALCGANERILCASPEYVASNPELT
CEEEEEECCCHHHHHCCCEEEEEEECCCCCCCEEEEEEECCCCEEEECCCCHHCCCCCCC
KPEDLHQHNCLCFMVADSVYNKWTLTREGETEQVVVSGKLLSNDSDLSHRLAIKGKGIAN
CCHHHHCCCEEEEEEECHHCCCEEEEECCCCCEEEEEEEEECCCCCCCEEEEEECCCCCC
KSLMDISQDLIEGRLVRVLPEWDCGSVPLYMVCADRRLLTPTIRTFRDFIQEKCCQQRKS
HHHHHHHHHHHCCEEEEEECCCCCCCCCEEEEECCCEECCHHHHHHHHHHHHHHHHHHHH
VLTTFCSER
HHHHHHCCC
>Mature Secondary Structure
MKALNDLNIFVETARQGSFSKAANSMDMTPAAISASIKRLEGQIGFPLFVRSTRSLRLTS
CCCCHHHHHEEECCCCCCHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCEEEEEC
EGELFLDKTTQALATLQEGLDQISSARGELSGQLHITAPSDFGRNMLLDWVDEFIELYPN
CCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCC
VSIKLELSDSLTDMYTKPVDIAIRYGEPADSNLVVLALCGANERILCASPEYVASNPELT
CEEEEEECCCHHHHHCCCEEEEEEECCCCCCCEEEEEEECCCCEEEECCCCHHCCCCCCC
KPEDLHQHNCLCFMVADSVYNKWTLTREGETEQVVVSGKLLSNDSDLSHRLAIKGKGIAN
CCHHHHCCCEEEEEEECHHCCCEEEEECCCCCEEEEEEEEECCCCCCCEEEEEECCCCCC
KSLMDISQDLIEGRLVRVLPEWDCGSVPLYMVCADRRLLTPTIRTFRDFIQEKCCQQRKS
HHHHHHHHHHHCCEEEEEECCCCCCCCCEEEEECCCEECCHHHHHHHHHHHHHHHHHHHH
VLTTFCSER
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]