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The map label for this gene is ansB [H]

Identifier: 156977727

GI number: 156977727

Start: 1669995

End: 1671011

Strand: Direct

Name: ansB [H]

Synonym: VIBHAR_06515

Alternate gene names: 156977727

Gene position: 1669995-1671011 (Clockwise)

Preceding gene: 156977726

Following gene: 156977729

Centisome position: 75.77

GC content: 48.28

Gene sequence:

>1017_bases
ATGAGCTCATTGGCTTTTGCAAATGCTGATTTACCTAACATCAAGATCTTAGCAACCGGCGGCACTATCGCTGGTGCAGG
GCAATCCGCCACTGAATCCAACTACACCGCTGGTAAGGTTGGAGTCGATTCACTGATCAACGCAGTGCCTAGCATGACCA
AAATTGCCGACATTTCCGGTGAACAGGTGGTCAGCATCGGCTCTCAGGACATGAACGATGAAGTCTGGCTAACGTTAGCC
AAGCGTGTGAATGAACTGCTCGCTCAGGACGACGTAGATGGCATTGTGATTACTCATGGTACCGACACCTTAGAAGAGAC
CGCCTACTTCCTAGATTTAACGGTGAAGAGTGACAAGCCTGTTGTGATCGTAGGTGCAATGCGCCCTTCTACGGCAATGA
GTGCTGACGGCCCAGTTAACCTCTACAACGCAGTGGTGACCGCAACGGATGAAGATTCTCAAGGTCGAGGCGTGCTAGTC
GCAATGAACGACACCATTTTTGATGCGCGTGATGTAACGAAAACCAACACAACATCAGTCAGTACTTTCCAATCACCGAA
CTTTGGCCCACTCGGTTACATTCACAACAGCGATGCGAAGTACCAACGCAGCCCAGAGCGCAAACACACCACTGAAACAA
CCTTCGATGTTTCCAAGCTCGATAAGCTGCCACAAGTCGGAATCGTTTATAACTATGCCAATGCTTCTAGCTTACCTGTG
AAAGCATTCGTCGATGCGAAGTTTGATGGCATCGTCAGCGCAGGTGTCGGCAATGGTAACTTGTACCACACGGTATTTGA
TGAGTTAGAAAAGGCGAGTAAAGATGGCATCATGGTTGTTCGCAGCTCTCGCACGCCTACCGGCTCTACTACCTTAGATG
CGGAAATTGATGACGATAAATACGGTTTCGTGGCATCTGGCACGTTGAATCCACAAAAAGCTCGTATTTTGTTGATGCTA
TCGCTAACACAAACCCAAAATTACCAAGACATCCAGCAGATGTTCCAACATTACTAA

Upstream 100 bases:

>100_bases
TTTAGGATAGATAACGCTTCCCCCAACATCTAACCCAAAACAGGTGATTCCTATGACCAAAAAGTTTCTCCGCCTCAGTT
CGATAACACTCGGTTTATGC

Downstream 100 bases:

>100_bases
TCGGACGGATCGCTAACTTTCCGATGAGTAAAAGCTAGCCGCTAGTTAAAAAGAGGAGACGCAGATATCTCCTCTTTTGT
GCTTGAGCATTGATAACTTA

Product: L-asparaginase II

Products: NA

Alternate protein names: L-asparaginase II; L-ASNase II; L-asparagine amidohydrolase II; Colaspase [H]

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MSSLAFANADLPNIKILATGGTIAGAGQSATESNYTAGKVGVDSLINAVPSMTKIADISGEQVVSIGSQDMNDEVWLTLA
KRVNELLAQDDVDGIVITHGTDTLEETAYFLDLTVKSDKPVVIVGAMRPSTAMSADGPVNLYNAVVTATDEDSQGRGVLV
AMNDTIFDARDVTKTNTTSVSTFQSPNFGPLGYIHNSDAKYQRSPERKHTTETTFDVSKLDKLPQVGIVYNYANASSLPV
KAFVDAKFDGIVSAGVGNGNLYHTVFDELEKASKDGIMVVRSSRTPTGSTTLDAEIDDDKYGFVASGTLNPQKARILLML
SLTQTQNYQDIQQMFQHY

Sequences:

>Translated_338_residues
MSSLAFANADLPNIKILATGGTIAGAGQSATESNYTAGKVGVDSLINAVPSMTKIADISGEQVVSIGSQDMNDEVWLTLA
KRVNELLAQDDVDGIVITHGTDTLEETAYFLDLTVKSDKPVVIVGAMRPSTAMSADGPVNLYNAVVTATDEDSQGRGVLV
AMNDTIFDARDVTKTNTTSVSTFQSPNFGPLGYIHNSDAKYQRSPERKHTTETTFDVSKLDKLPQVGIVYNYANASSLPV
KAFVDAKFDGIVSAGVGNGNLYHTVFDELEKASKDGIMVVRSSRTPTGSTTLDAEIDDDKYGFVASGTLNPQKARILLML
SLTQTQNYQDIQQMFQHY
>Mature_337_residues
SSLAFANADLPNIKILATGGTIAGAGQSATESNYTAGKVGVDSLINAVPSMTKIADISGEQVVSIGSQDMNDEVWLTLAK
RVNELLAQDDVDGIVITHGTDTLEETAYFLDLTVKSDKPVVIVGAMRPSTAMSADGPVNLYNAVVTATDEDSQGRGVLVA
MNDTIFDARDVTKTNTTSVSTFQSPNFGPLGYIHNSDAKYQRSPERKHTTETTFDVSKLDKLPQVGIVYNYANASSLPVK
AFVDAKFDGIVSAGVGNGNLYHTVFDELEKASKDGIMVVRSSRTPTGSTTLDAEIDDDKYGFVASGTLNPQKARILLMLS
LTQTQNYQDIQQMFQHY

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=230, Percent_Identity=27.8260869565217, Blast_Score=79, Evalue=8e-15,
Organism=Escherichia coli, GI1789327, Length=328, Percent_Identity=73.4756097560976, Blast_Score=476, Evalue=1e-136,
Organism=Escherichia coli, GI1788065, Length=288, Percent_Identity=28.125, Blast_Score=89, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17558278, Length=232, Percent_Identity=26.7241379310345, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI193207372, Length=232, Percent_Identity=28.0172413793103, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI193207374, Length=232, Percent_Identity=26.7241379310345, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17558280, Length=232, Percent_Identity=26.7241379310345, Blast_Score=75, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6323189, Length=335, Percent_Identity=43.5820895522388, Blast_Score=232, Evalue=7e-62,
Organism=Saccharomyces cerevisiae, GI6323187, Length=335, Percent_Identity=43.5820895522388, Blast_Score=232, Evalue=7e-62,
Organism=Saccharomyces cerevisiae, GI6323186, Length=335, Percent_Identity=43.5820895522388, Blast_Score=232, Evalue=7e-62,
Organism=Saccharomyces cerevisiae, GI6323184, Length=335, Percent_Identity=43.5820895522388, Blast_Score=232, Evalue=7e-62,
Organism=Saccharomyces cerevisiae, GI6320527, Length=346, Percent_Identity=39.0173410404624, Blast_Score=207, Evalue=2e-54,
Organism=Drosophila melanogaster, GI24645530, Length=238, Percent_Identity=28.9915966386555, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24639609, Length=232, Percent_Identity=27.1551724137931, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004550
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 36269; Mature: 36138

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLAFANADLPNIKILATGGTIAGAGQSATESNYTAGKVGVDSLINAVPSMTKIADISG
CCCCCCCCCCCCCEEEEEECCEEECCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHCCCC
EQVVSIGSQDMNDEVWLTLAKRVNELLAQDDVDGIVITHGTDTLEETAYFLDLTVKSDKP
CEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHCEEEEEEEECCCCC
VVIVGAMRPSTAMSADGPVNLYNAVVTATDEDSQGRGVLVAMNDTIFDARDVTKTNTTSV
EEEEECCCCCCCCCCCCCCEEEEEEEEECCCCCCCCEEEEEECCCEECCCCCCCCCCCEE
STFQSPNFGPLGYIHNSDAKYQRSPERKHTTETTFDVSKLDKLPQVGIVYNYANASSLPV
EEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEHHHHHCCCCCEEEEEECCCCCCCE
KAFVDAKFDGIVSAGVGNGNLYHTVFDELEKASKDGIMVVRSSRTPTGSTTLDAEIDDDK
EEEECCCCCCEEEECCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEEECCCC
YGFVASGTLNPQKARILLMLSLTQTQNYQDIQQMFQHY
CCEEEECCCCCCCEEEEEEEEECCCCCHHHHHHHHHCC
>Mature Secondary Structure 
SSLAFANADLPNIKILATGGTIAGAGQSATESNYTAGKVGVDSLINAVPSMTKIADISG
CCCCCCCCCCCCEEEEEECCEEECCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHCCCC
EQVVSIGSQDMNDEVWLTLAKRVNELLAQDDVDGIVITHGTDTLEETAYFLDLTVKSDKP
CEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHCEEEEEEEECCCCC
VVIVGAMRPSTAMSADGPVNLYNAVVTATDEDSQGRGVLVAMNDTIFDARDVTKTNTTSV
EEEEECCCCCCCCCCCCCCEEEEEEEEECCCCCCCCEEEEEECCCEECCCCCCCCCCCEE
STFQSPNFGPLGYIHNSDAKYQRSPERKHTTETTFDVSKLDKLPQVGIVYNYANASSLPV
EEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEHHHHHCCCCCEEEEEECCCCCCCE
KAFVDAKFDGIVSAGVGNGNLYHTVFDELEKASKDGIMVVRSSRTPTGSTTLDAEIDDDK
EEEECCCCCCEEEECCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEEECCCC
YGFVASGTLNPQKARILLMLSLTQTQNYQDIQQMFQHY
CCEEEECCCCCCCEEEEEEEEECCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2407723; 2144836; 9278503; 6766894; 387570; 321449; 4561256; 1906013; 1521538; 1287659; 8434007; 8706862 [H]