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The map label for this gene is purA2 [H]

Identifier: 156977724

GI number: 156977724

Start: 1666072

End: 1667328

Strand: Direct

Name: purA2 [H]

Synonym: VIBHAR_06512

Alternate gene names: 156977724

Gene position: 1666072-1667328 (Clockwise)

Preceding gene: 156977719

Following gene: 156977726

Centisome position: 75.59

GC content: 49.24

Gene sequence:

>1257_bases
ATGTCGTCTATCGTTGTTGTGGGTGCAAACTGGGGTGATGAAGGCAAAGGCCGCATCGTTGACTTTTTGGCAGAGCAAGC
TTCTGCAAGCATTCGTTTCCAAGGTGGTAACAACGCGGGTCACACCGTAGTTAACGACCTAGGTACGTTCAAGCTGCACC
AGCTACCAAGTGGCGTTTTCAACCCTGACTGTTTGGCAGTTTTAGGTCCGGGTATGGTTATCAGCCCTGCGTCGCTGTCT
CAGGAGATTGCGGAAGTCGCGGAAGCGGGCGTTAAAGTGAACATGTGCATCTCTGATCGCGCAACACTGTGTCTACCTCT
ACACGCACTAGAAGATACGCTAGAAGAACAACGCTTAGGCGACAAAGCATACGGTTCAACTCGTCAAGGTATTGCACCTG
CATACGGCGACCGTGTGATGAAAAAAGGCATCCTAGTTGGTTGGTTAAAACAACCAGAAGTTCTAGTAGAACGCATCAAG
TTCTGGATGGACTGGAAACTTCCACAACTACGTGCCCTTTACCCAACGTTCGAATTCGAGCAAACCGCTGAAGAAATGGC
GAACTGGTTGCTAGAAGTATCTGCACCTTGGCGTGATGCTATCTGCAACGTAACGCTGCCGCTAAAAGAGCTGCAAGCAA
AAGACCAAACTCTACTGTTCGAAGCACAACTAGGTGCGGGCCGTGACCTTGTTTACGGAGAATACCCATGGACAACATCT
TCAAACGTTGTGTCTATGTACGCAGGTATCGGTAGTGGTCTTCCTGCTCTGCGTCCAGAGCGTGTTATTGCAGTCGCAAA
AGCGTTCAGCTCATCAGTAGGTACTGGTACGCTAATCACTGCAATGGAAGAGCAAGATGCATTCCGTGAACTAGCGGGTG
AGTTCGGCGCAACAACAGGTCGCCCACGTGACATGGGTTACTTCGATGCAGTAGCGACTAAGAACGGCGTTGAGCTTCAG
GCAGCGACTGAAATCGCACTGACAAAAGTAGACTGTCTGACAGGTCTTAAAGATCTAAAAATCTGTGTTGGTTACGAAGG
CGAGCACAGTGAGAACCCAATTTGGCCTCAAACGGCTGCACTAGCACCAATCTACGAGAAGATGGAAAGCTGGAGTGAAG
ACATTACCGGTTGCCGTAAATTCGAAGAGCTACCAGTTGCGGCACAAAAATACGTTCTACGTATTGAAGAGCTAATGGGC
GTACCAGTGAAAATGGTATCGGTTGGCCCTGGTCGTGAACAAATGATTATGCGCTAA

Upstream 100 bases:

>100_bases
TACCCGATATGATTTTCTTATCAAGTTAATGAGTATAATTAATTTCTCTTATTCATGCGAGCATCCTAAGATTTTCCGGT
CTGTTTTTGGAGAATGAATT

Downstream 100 bases:

>100_bases
GACGTGGAATAACACGTCTATATCCAAGTTACAGTAAGTGCTTGGTTATATTAGCAAGCGGCTTTTATCTTTTCCGGGGT
AAAGCCGCTTTTTTTATGCA

Product: adenylosuccinate synthase

Products: NA

Alternate protein names: AMPSase 2; AdSS 2; IMP--aspartate ligase 2 [H]

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MSSIVVVGANWGDEGKGRIVDFLAEQASASIRFQGGNNAGHTVVNDLGTFKLHQLPSGVFNPDCLAVLGPGMVISPASLS
QEIAEVAEAGVKVNMCISDRATLCLPLHALEDTLEEQRLGDKAYGSTRQGIAPAYGDRVMKKGILVGWLKQPEVLVERIK
FWMDWKLPQLRALYPTFEFEQTAEEMANWLLEVSAPWRDAICNVTLPLKELQAKDQTLLFEAQLGAGRDLVYGEYPWTTS
SNVVSMYAGIGSGLPALRPERVIAVAKAFSSSVGTGTLITAMEEQDAFRELAGEFGATTGRPRDMGYFDAVATKNGVELQ
AATEIALTKVDCLTGLKDLKICVGYEGEHSENPIWPQTAALAPIYEKMESWSEDITGCRKFEELPVAAQKYVLRIEELMG
VPVKMVSVGPGREQMIMR

Sequences:

>Translated_418_residues
MSSIVVVGANWGDEGKGRIVDFLAEQASASIRFQGGNNAGHTVVNDLGTFKLHQLPSGVFNPDCLAVLGPGMVISPASLS
QEIAEVAEAGVKVNMCISDRATLCLPLHALEDTLEEQRLGDKAYGSTRQGIAPAYGDRVMKKGILVGWLKQPEVLVERIK
FWMDWKLPQLRALYPTFEFEQTAEEMANWLLEVSAPWRDAICNVTLPLKELQAKDQTLLFEAQLGAGRDLVYGEYPWTTS
SNVVSMYAGIGSGLPALRPERVIAVAKAFSSSVGTGTLITAMEEQDAFRELAGEFGATTGRPRDMGYFDAVATKNGVELQ
AATEIALTKVDCLTGLKDLKICVGYEGEHSENPIWPQTAALAPIYEKMESWSEDITGCRKFEELPVAAQKYVLRIEELMG
VPVKMVSVGPGREQMIMR
>Mature_417_residues
SSIVVVGANWGDEGKGRIVDFLAEQASASIRFQGGNNAGHTVVNDLGTFKLHQLPSGVFNPDCLAVLGPGMVISPASLSQ
EIAEVAEAGVKVNMCISDRATLCLPLHALEDTLEEQRLGDKAYGSTRQGIAPAYGDRVMKKGILVGWLKQPEVLVERIKF
WMDWKLPQLRALYPTFEFEQTAEEMANWLLEVSAPWRDAICNVTLPLKELQAKDQTLLFEAQLGAGRDLVYGEYPWTTSS
NVVSMYAGIGSGLPALRPERVIAVAKAFSSSVGTGTLITAMEEQDAFRELAGEFGATTGRPRDMGYFDAVATKNGVELQA
ATEIALTKVDCLTGLKDLKICVGYEGEHSENPIWPQTAALAPIYEKMESWSEDITGCRKFEELPVAAQKYVLRIEELMGV
PVKMVSVGPGREQMIMR

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI34577063, Length=432, Percent_Identity=34.0277777777778, Blast_Score=231, Evalue=1e-60,
Organism=Homo sapiens, GI22748717, Length=428, Percent_Identity=34.3457943925234, Blast_Score=212, Evalue=5e-55,
Organism=Homo sapiens, GI40316944, Length=399, Percent_Identity=32.3308270676692, Blast_Score=176, Evalue=3e-44,
Organism=Escherichia coli, GI1790620, Length=427, Percent_Identity=36.768149882904, Blast_Score=271, Evalue=5e-74,
Organism=Caenorhabditis elegans, GI25146553, Length=437, Percent_Identity=32.7231121281465, Blast_Score=219, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI25146555, Length=437, Percent_Identity=32.7231121281465, Blast_Score=219, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6324109, Length=435, Percent_Identity=32.8735632183908, Blast_Score=218, Evalue=1e-57,
Organism=Drosophila melanogaster, GI21356033, Length=425, Percent_Identity=36, Blast_Score=222, Evalue=4e-58,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 45637; Mature: 45506

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSIVVVGANWGDEGKGRIVDFLAEQASASIRFQGGNNAGHTVVNDLGTFKLHQLPSGVF
CCCEEEEECCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCHHHHHCCCEEHHHCCCCCC
NPDCLAVLGPGMVISPASLSQEIAEVAEAGVKVNMCISDRATLCLPLHALEDTLEEQRLG
CCCHHHHCCCCCEECCHHHHHHHHHHHHCCCEEEEEECCCCEEEEEHHHHHHHHHHHHCC
DKAYGSTRQGIAPAYGDRVMKKGILVGWLKQPEVLVERIKFWMDWKLPQLRALYPTFEFE
CHHCCCCCCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHCCCCCHHHHHCCCCHHH
QTAEEMANWLLEVSAPWRDAICNVTLPLKELQAKDQTLLFEAQLGAGRDLVYGEYPWTTS
HHHHHHHHHHEECCCCHHHHHCCEECCHHHHCCCCCEEEEEECCCCCCCEEECCCCCCCC
SNVVSMYAGIGSGLPALRPERVIAVAKAFSSSVGTGTLITAMEEQDAFRELAGEFGATTG
CCHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHCCCCC
RPRDMGYFDAVATKNGVELQAATEIALTKVDCLTGLKDLKICVGYEGEHSENPIWPQTAA
CCCCCCHHHHHHCCCCCEEEEHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHH
LAPIYEKMESWSEDITGCRKFEELPVAAQKYVLRIEELMGVPVKMVSVGPGREQMIMR
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEECCCCHHHHCCC
>Mature Secondary Structure 
SSIVVVGANWGDEGKGRIVDFLAEQASASIRFQGGNNAGHTVVNDLGTFKLHQLPSGVF
CCEEEEECCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCHHHHHCCCEEHHHCCCCCC
NPDCLAVLGPGMVISPASLSQEIAEVAEAGVKVNMCISDRATLCLPLHALEDTLEEQRLG
CCCHHHHCCCCCEECCHHHHHHHHHHHHCCCEEEEEECCCCEEEEEHHHHHHHHHHHHCC
DKAYGSTRQGIAPAYGDRVMKKGILVGWLKQPEVLVERIKFWMDWKLPQLRALYPTFEFE
CHHCCCCCCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHCCCCCHHHHHCCCCHHH
QTAEEMANWLLEVSAPWRDAICNVTLPLKELQAKDQTLLFEAQLGAGRDLVYGEYPWTTS
HHHHHHHHHHEECCCCHHHHHCCEECCHHHHCCCCCEEEEEECCCCCCCEEECCCCCCCC
SNVVSMYAGIGSGLPALRPERVIAVAKAFSSSVGTGTLITAMEEQDAFRELAGEFGATTG
CCHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHCCCCC
RPRDMGYFDAVATKNGVELQAATEIALTKVDCLTGLKDLKICVGYEGEHSENPIWPQTAA
CCCCCCHHHHHHCCCCCEEEEHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHH
LAPIYEKMESWSEDITGCRKFEELPVAAQKYVLRIEELMGVPVKMVSVGPGREQMIMR
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEECCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA