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The map label for this gene is nemA [H]

Identifier: 156977710

GI number: 156977710

Start: 1650629

End: 1651720

Strand: Direct

Name: nemA [H]

Synonym: VIBHAR_06498

Alternate gene names: 156977710

Gene position: 1650629-1651720 (Clockwise)

Preceding gene: 156977709

Following gene: 156977711

Centisome position: 74.89

GC content: 50.55

Gene sequence:

>1092_bases
ATGAGCAAACTATTTGAACCAGCACAGCTAAAACAACTGGATCTACAAAACCGCGTAGTCATGGCACCAATGACCCGAGC
GCGCACCAGCCAACCGGGGAATATCCCAAACCAAATGATGGCGACTTACTACAAGCAGCGCGCAACCGCAGGGTTAATCA
TCTCTGAAGCCACCCAGATTTCAGACGATTCGCAGGGTTACTCGTTCACACCAGGCGTCTACACTGATGAACAAGTGGCA
GGCTGGAAAACCGTCACTCAAGCAGCAAAAGAGCAAGGCGCAGCGATGTTTTGTCAGTTATGGCATGTTGGCCGTGTATC
ACACCCGACATTCCAAAAAGGCGAACAGCCTATTGCCCCATCAGCACTAAAACCCGTAGAAACCCAAGTGTGGATTGCCG
ACGAACAAGGCAATGGCAGCATGGTCGATTGTGTAGAGCCACGAGCCATGACTCAAGCCGATATTGACCGTGTGGTAAGG
GATTTTGCCCATGCAGCGAAACGTGCGATTGAAGCTGGCTTCGATGGCGTCGAAATCCACGGCGGTAATGGTTACCTGAT
TGACCAGTTCCTACGTACCAATTCAAACCACAGAACCGATAACTACGGCGGAACTCGCGAAAACCGACTTCGCTTTTTGT
TAGAAGTGGTCGATGCGGTGAGCGAAGCCATTGGGGCCAACAAAGTGGGAGTACGACTAGCGCCATTCATCACTTTCAAG
GACATGAACTGCCCAGACATTGTGCCAACCATCCTCGAGGCATCGAAACAACTTCAAGCTCGTGATATTGCGTACTTGCA
TCTGTCTGAAGCGGATTGGGACGACGCACCAGTGATTCCAGAAAGCTTCCGCATCGAACTGCGCGAATACTTCACCAATA
CGATTATCGTGGCTGGCAGTTACACCCAAGATCGTGCTGACGAGGTACTTGAAAAAGGTTATGCGGATTTAGTCGCTTTT
GGACGCCCGTTTGTTTCTAACCCAGATTTGGTGGCGCGCTTACAGCATCAACAACCTCTGGCAGAACTGGATGGTTCAAC
ACTATTTGGTGGCAATGAACACGGCTATACCGATTATCCGGCGCTTGGTTGA

Upstream 100 bases:

>100_bases
TTCAGTGCCGCAGAAATCCAATGGGACAACACCGTGAAAGATTGGATGGAATCGGTAAGCAGCAAAACCCAAGTGCCACT
GCTTAAGTAAGGAGCCGATA

Downstream 100 bases:

>100_bases
AAGGTTTTACCCGCTTCTGAAAAGGCTTTACCCGCTTCTGATAGCGATTCATAGCGTGCCGTATTTCTCAAATTAATCAG
TTGTTATCCCATAAAGAGAA

Product: NADH-flavin oxidoreductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MSKLFEPAQLKQLDLQNRVVMAPMTRARTSQPGNIPNQMMATYYKQRATAGLIISEATQISDDSQGYSFTPGVYTDEQVA
GWKTVTQAAKEQGAAMFCQLWHVGRVSHPTFQKGEQPIAPSALKPVETQVWIADEQGNGSMVDCVEPRAMTQADIDRVVR
DFAHAAKRAIEAGFDGVEIHGGNGYLIDQFLRTNSNHRTDNYGGTRENRLRFLLEVVDAVSEAIGANKVGVRLAPFITFK
DMNCPDIVPTILEASKQLQARDIAYLHLSEADWDDAPVIPESFRIELREYFTNTIIVAGSYTQDRADEVLEKGYADLVAF
GRPFVSNPDLVARLQHQQPLAELDGSTLFGGNEHGYTDYPALG

Sequences:

>Translated_363_residues
MSKLFEPAQLKQLDLQNRVVMAPMTRARTSQPGNIPNQMMATYYKQRATAGLIISEATQISDDSQGYSFTPGVYTDEQVA
GWKTVTQAAKEQGAAMFCQLWHVGRVSHPTFQKGEQPIAPSALKPVETQVWIADEQGNGSMVDCVEPRAMTQADIDRVVR
DFAHAAKRAIEAGFDGVEIHGGNGYLIDQFLRTNSNHRTDNYGGTRENRLRFLLEVVDAVSEAIGANKVGVRLAPFITFK
DMNCPDIVPTILEASKQLQARDIAYLHLSEADWDDAPVIPESFRIELREYFTNTIIVAGSYTQDRADEVLEKGYADLVAF
GRPFVSNPDLVARLQHQQPLAELDGSTLFGGNEHGYTDYPALG
>Mature_362_residues
SKLFEPAQLKQLDLQNRVVMAPMTRARTSQPGNIPNQMMATYYKQRATAGLIISEATQISDDSQGYSFTPGVYTDEQVAG
WKTVTQAAKEQGAAMFCQLWHVGRVSHPTFQKGEQPIAPSALKPVETQVWIADEQGNGSMVDCVEPRAMTQADIDRVVRD
FAHAAKRAIEAGFDGVEIHGGNGYLIDQFLRTNSNHRTDNYGGTRENRLRFLLEVVDAVSEAIGANKVGVRLAPFITFKD
MNCPDIVPTILEASKQLQARDIAYLHLSEADWDDAPVIPESFRIELREYFTNTIIVAGSYTQDRADEVLEKGYADLVAFG
RPFVSNPDLVARLQHQQPLAELDGSTLFGGNEHGYTDYPALG

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=362, Percent_Identity=46.9613259668508, Blast_Score=337, Evalue=7e-94,
Organism=Escherichia coli, GI1789463, Length=354, Percent_Identity=28.8135593220339, Blast_Score=131, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI17565138, Length=358, Percent_Identity=27.6536312849162, Blast_Score=101, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17559802, Length=356, Percent_Identity=26.685393258427, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17540738, Length=274, Percent_Identity=29.1970802919708, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI72001454, Length=356, Percent_Identity=24.438202247191, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17559804, Length=278, Percent_Identity=28.4172661870504, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17564188, Length=267, Percent_Identity=25.8426966292135, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17566914, Length=163, Percent_Identity=32.5153374233129, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6325086, Length=379, Percent_Identity=38.7862796833773, Blast_Score=237, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6321973, Length=387, Percent_Identity=36.6925064599483, Blast_Score=214, Evalue=1e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 40186; Mature: 40054

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLFEPAQLKQLDLQNRVVMAPMTRARTSQPGNIPNQMMATYYKQRATAGLIISEATQI
CCCCCCCHHHHHHHHCCCEEECCCHHHCCCCCCCCHHHHHHHHHHHHHHHCEEEECHHHC
SDDSQGYSFTPGVYTDEQVAGWKTVTQAAKEQGAAMFCQLWHVGRVSHPTFQKGEQPIAP
CCCCCCCEECCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHCCCCCCCC
SALKPVETQVWIADEQGNGSMVDCVEPRAMTQADIDRVVRDFAHAAKRAIEAGFDGVEIH
CCCCCCCEEEEEEEECCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
GGNGYLIDQFLRTNSNHRTDNYGGTRENRLRFLLEVVDAVSEAIGANKVGVRLAPFITFK
CCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEC
DMNCPDIVPTILEASKQLQARDIAYLHLSEADWDDAPVIPESFRIELREYFTNTIIVAGS
CCCCCHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCEEEEECC
YTQDRADEVLEKGYADLVAFGRPFVSNPDLVARLQHQQPLAELDGSTLFGGNEHGYTDYP
CCHHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHCCCHHHCCCCEEECCCCCCCCCCC
ALG
CCC
>Mature Secondary Structure 
SKLFEPAQLKQLDLQNRVVMAPMTRARTSQPGNIPNQMMATYYKQRATAGLIISEATQI
CCCCCCHHHHHHHHCCCEEECCCHHHCCCCCCCCHHHHHHHHHHHHHHHCEEEECHHHC
SDDSQGYSFTPGVYTDEQVAGWKTVTQAAKEQGAAMFCQLWHVGRVSHPTFQKGEQPIAP
CCCCCCCEECCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHCCCCCCCC
SALKPVETQVWIADEQGNGSMVDCVEPRAMTQADIDRVVRDFAHAAKRAIEAGFDGVEIH
CCCCCCCEEEEEEEECCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
GGNGYLIDQFLRTNSNHRTDNYGGTRENRLRFLLEVVDAVSEAIGANKVGVRLAPFITFK
CCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEC
DMNCPDIVPTILEASKQLQARDIAYLHLSEADWDDAPVIPESFRIELREYFTNTIIVAGS
CCCCCHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCEEEEECC
YTQDRADEVLEKGYADLVAFGRPFVSNPDLVARLQHQQPLAELDGSTLFGGNEHGYTDYP
CCHHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHCCCHHHCCCCEEECCCCCCCCCCC
ALG
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]