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The map label for this gene is pfeS [H]

Identifier: 156977327

GI number: 156977327

Start: 1274177

End: 1275517

Strand: Direct

Name: pfeS [H]

Synonym: VIBHAR_06113

Alternate gene names: 156977327

Gene position: 1274177-1275517 (Clockwise)

Preceding gene: 156977326

Following gene: 156977328

Centisome position: 57.81

GC content: 46.16

Gene sequence:

>1341_bases
ATGAAGAAGATGCTGTTTTGGCGGTTGTTTGTCGTCTTATCTCTGGGCGTGGTGATCTTTTTCTCCTTGATTCACAGCGC
GGCCATCCTCTCCAACGAGAAAATGAGCTATTTGAAGAAATCTCATCGCCAACAAATTTTAGCCTGGGGACATTCCGCAC
AAACCTATGTAGACCAACAAGATTGGCAAGGACTGGACATCTGGTTAGAACAGCTCGCCATTGATGAGTCCACATGGGCG
ACAGTGCTGCAATCCCGTCTTGACGTAAAAGGGGGAAACCCACTTAACGAGCGTTTTTGGCAAGGCTATGGTATTGGCCG
AAGCGTTGAATGGAAGATCCATCTCGATTTTCCAGACAACCCAATTATGGAAGTCCCTCTGTCCCCTTCCGGATACCACT
TTTTGATTCTGTTACCTGACCGGATGAGACCGGGAACTTACATGTCTCACGCGTTTTGGTTGTTTCGCTTCGTTATTCCC
TTTCTTTCTCTACTCGCACTGACTGTTTATTTGTACCGCTATGTCATGCGCCCGTTACAGCAATTCCACCACGCAAGCCA
AGCTTTCAGTCAAGGCGATTACTCAGCGCGAATTGGTCAGACTATGAAGTTGGGCAATGATGAAATCAGCCAAGTCGCGC
GCACCTTCGATTATATGGCAGAGCGCACATCGGATGTGATTCGCCACAATCGTAACCTTATTGCCGATATGTCCCACGAG
ATACGCACTCCACTCGCTCGAATTGAGATGGCCATTGATTGTGTAGATAAGAATATCGAACGTGAACAAATGTTGGAGCG
AATCAAGAGCGATACCCACAAAATGCGCACGACCGCAGAAGACACACTCACACTAGCATGGATTGAAAACGAGCAACCAA
ATCTTAGGACAGAGGAATTTGACTTAGCTGAACTAGTTGAAATCATCGTCGAAGATGCCCGATTCGAGTACTCAGATAGA
CAGATATCACTACATCAAGACGAACCATTACCCATCCAGCACTCCAATCAAATGGCTCTCAGTCAAGCATTGGAAAACGT
CATTCGTAATGGCCTACGATACAACCAACCCGATGAGATACTCGAAGTTCGAATACTAAACCGCCTCGAACATTATCAAA
TCCTCATCATTGATTCTGGCCCAGGTATAGACCCGAGTTTATACCAACAGATATTCAAACCTTTCTTCAAGGCCAACAAT
CAAGTCGTATCCCGAAAAGGCTTTGGTGTTGGATTAGCATTGGCGAAACGACACATTGAAGCGGTTGGCGGCAAGATCGT
TGCCTACAACGCACATGTGCAAGGTTTAACTATGGATATTACCTTGCCTAAGCGAGTTTAA

Upstream 100 bases:

>100_bases
CGCTCGACATGCATCTAAGCCGTGTGCGTAAGCGTCTTATTGCTGAGGGTATGTCGAATGACCGAATCCAAACCGTGCAT
GGTAAAGGATATATTTTTAA

Downstream 100 bases:

>100_bases
CGTCTGCTGACTACAAGGATTGTAACAGTTGTAAAAGTCGTTTACTCAGAGTGGAAGAATCACCAAAATCTCACTCGATA
ATAATTCTCATTAACAATGG

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MKKMLFWRLFVVLSLGVVIFFSLIHSAAILSNEKMSYLKKSHRQQILAWGHSAQTYVDQQDWQGLDIWLEQLAIDESTWA
TVLQSRLDVKGGNPLNERFWQGYGIGRSVEWKIHLDFPDNPIMEVPLSPSGYHFLILLPDRMRPGTYMSHAFWLFRFVIP
FLSLLALTVYLYRYVMRPLQQFHHASQAFSQGDYSARIGQTMKLGNDEISQVARTFDYMAERTSDVIRHNRNLIADMSHE
IRTPLARIEMAIDCVDKNIEREQMLERIKSDTHKMRTTAEDTLTLAWIENEQPNLRTEEFDLAELVEIIVEDARFEYSDR
QISLHQDEPLPIQHSNQMALSQALENVIRNGLRYNQPDEILEVRILNRLEHYQILIIDSGPGIDPSLYQQIFKPFFKANN
QVVSRKGFGVGLALAKRHIEAVGGKIVAYNAHVQGLTMDITLPKRV

Sequences:

>Translated_446_residues
MKKMLFWRLFVVLSLGVVIFFSLIHSAAILSNEKMSYLKKSHRQQILAWGHSAQTYVDQQDWQGLDIWLEQLAIDESTWA
TVLQSRLDVKGGNPLNERFWQGYGIGRSVEWKIHLDFPDNPIMEVPLSPSGYHFLILLPDRMRPGTYMSHAFWLFRFVIP
FLSLLALTVYLYRYVMRPLQQFHHASQAFSQGDYSARIGQTMKLGNDEISQVARTFDYMAERTSDVIRHNRNLIADMSHE
IRTPLARIEMAIDCVDKNIEREQMLERIKSDTHKMRTTAEDTLTLAWIENEQPNLRTEEFDLAELVEIIVEDARFEYSDR
QISLHQDEPLPIQHSNQMALSQALENVIRNGLRYNQPDEILEVRILNRLEHYQILIIDSGPGIDPSLYQQIFKPFFKANN
QVVSRKGFGVGLALAKRHIEAVGGKIVAYNAHVQGLTMDITLPKRV
>Mature_446_residues
MKKMLFWRLFVVLSLGVVIFFSLIHSAAILSNEKMSYLKKSHRQQILAWGHSAQTYVDQQDWQGLDIWLEQLAIDESTWA
TVLQSRLDVKGGNPLNERFWQGYGIGRSVEWKIHLDFPDNPIMEVPLSPSGYHFLILLPDRMRPGTYMSHAFWLFRFVIP
FLSLLALTVYLYRYVMRPLQQFHHASQAFSQGDYSARIGQTMKLGNDEISQVARTFDYMAERTSDVIRHNRNLIADMSHE
IRTPLARIEMAIDCVDKNIEREQMLERIKSDTHKMRTTAEDTLTLAWIENEQPNLRTEEFDLAELVEIIVEDARFEYSDR
QISLHQDEPLPIQHSNQMALSQALENVIRNGLRYNQPDEILEVRILNRLEHYQILIIDSGPGIDPSLYQQIFKPFFKANN
QVVSRKGFGVGLALAKRHIEAVGGKIVAYNAHVQGLTMDITLPKRV

Specific function: Member of the two-component regulatory system pfeR/pfeS. May activate pfeR by phosphorylation [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790346, Length=272, Percent_Identity=27.9411764705882, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1788393, Length=275, Percent_Identity=22.5454545454545, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787894, Length=311, Percent_Identity=25.7234726688103, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786783, Length=272, Percent_Identity=26.8382352941176, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI87081816, Length=339, Percent_Identity=25.9587020648968, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1789808, Length=293, Percent_Identity=24.9146757679181, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1786912, Length=222, Percent_Identity=27.4774774774775, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1786600, Length=224, Percent_Identity=24.1071428571429, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1790861, Length=250, Percent_Identity=24.4, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI87082128, Length=300, Percent_Identity=20.3333333333333, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 51696; Mature: 51696

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKMLFWRLFVVLSLGVVIFFSLIHSAAILSNEKMSYLKKSHRQQILAWGHSAQTYVDQQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCC
DWQGLDIWLEQLAIDESTWATVLQSRLDVKGGNPLNERFWQGYGIGRSVEWKIHLDFPDN
CCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCEEEEEEEEECCCC
PIMEVPLSPSGYHFLILLPDRMRPGTYMSHAFWLFRFVIPFLSLLALTVYLYRYVMRPLQ
CCEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QFHHASQAFSQGDYSARIGQTMKLGNDEISQVARTFDYMAERTSDVIRHNRNLIADMSHE
HHHHHHHHHHCCCCHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
IRTPLARIEMAIDCVDKNIEREQMLERIKSDTHKMRTTAEDTLTLAWIENEQPNLRTEEF
HHCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCC
DLAELVEIIVEDARFEYSDRQISLHQDEPLPIQHSNQMALSQALENVIRNGLRYNQPDEI
CHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHH
LEVRILNRLEHYQILIIDSGPGIDPSLYQQIFKPFFKANNQVVSRKGFGVGLALAKRHIE
HHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHH
AVGGKIVAYNAHVQGLTMDITLPKRV
HHCCEEEEEECEECEEEEEEECCCCC
>Mature Secondary Structure
MKKMLFWRLFVVLSLGVVIFFSLIHSAAILSNEKMSYLKKSHRQQILAWGHSAQTYVDQQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCC
DWQGLDIWLEQLAIDESTWATVLQSRLDVKGGNPLNERFWQGYGIGRSVEWKIHLDFPDN
CCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCEEEEEEEEECCCC
PIMEVPLSPSGYHFLILLPDRMRPGTYMSHAFWLFRFVIPFLSLLALTVYLYRYVMRPLQ
CCEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QFHHASQAFSQGDYSARIGQTMKLGNDEISQVARTFDYMAERTSDVIRHNRNLIADMSHE
HHHHHHHHHHCCCCHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
IRTPLARIEMAIDCVDKNIEREQMLERIKSDTHKMRTTAEDTLTLAWIENEQPNLRTEEF
HHCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCC
DLAELVEIIVEDARFEYSDRQISLHQDEPLPIQHSNQMALSQALENVIRNGLRYNQPDEI
CHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHH
LEVRILNRLEHYQILIIDSGPGIDPSLYQQIFKPFFKANNQVVSRKGFGVGLALAKRHIE
HHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHH
AVGGKIVAYNAHVQGLTMDITLPKRV
HHCCEEEEEECEECEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8361354; 10984043 [H]