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The map label for this gene is cqsA [H]

Identifier: 156977302

GI number: 156977302

Start: 1250747

End: 1252048

Strand: Direct

Name: cqsA [H]

Synonym: VIBHAR_06088

Alternate gene names: 156977302

Gene position: 1250747-1252048 (Clockwise)

Preceding gene: 156977301

Following gene: 156977304

Centisome position: 56.75

GC content: 46.7

Gene sequence:

>1302_bases
ATGCGGAGTTTGCAGGCGCACGCAAACTTATGCAACTTGACTAATGACACCGAACTCATATTAGTCAAACTATATAACTG
TCAGCGCTGGCGTTTTCCTGTGTTACGAAGAGAACTTATTATGAGTGATAAACCGAAAACAAAACCACTTCCTTCCTTTG
TTGAGGGACGCCTAGACTTTTATATTCAAGATCTGATTGAGCAGAACGAAAACCAAAAGCACTTGGTATTAGGGAAGCGA
CCACAACAAGGAGCGGTGGTCATGCAAAGTAACGACTACTTGTCGTTGTCCCACAATCTACAAATTCAACAGGCACATCG
TGATGCTATCTACGAGCACGATGATAACGTTGTCATGTCTGCGATTTTCTTACAAGATGACGACTCAAAACCTGCATTTG
AAACGCAGCTCGCGGAGTATGTCGGTATGGGCAGTTGCCTATTATCCCAGTCCGGATGGGCAGCCAATATTGGCTTGTTA
CAAACGATTTGTCCGCCAGAAACACCAGTCTATATCGATTTCTTTGCACACATGTCTTTGTGGGAAGGGATTCGAGCGGC
AGGAGCGCAAGCGCATCCATTCATGCATAACAACATGAACCATTTGCGCAAGCAGATACAGCGTAACGGCTCTGGCGTTA
TCGTTGTGGATTCGGTCTATAGCACGATAGGTACGATCGCCCCACTCCGCGACATCTACGAAATGGCGCGTGAGTTTGAT
TGTGCTTTGGTGGTCGATGAGTCCCACTCATTAGGCACGCATGGGCCAAATGGTTCTGGCTTGGTGAAAGCATTGGAACT
CACTGAGCAAGTGGATTTCATTACGGTGAGTTTGGCAAAAACATTTGCTTATCGCGCCGGGGCGATTCTTGGTCCAGAAA
AGTTGGCTAGAACGCTACCCTTTGTCGCATTCCCAGCCATCTTTAGTTCGACGGTCCTTCCACAAGAGATCGTTCGACTT
GAAAAGACTCTCGAAGTCATTAGAAGCGCAGATGATAAGCGAACCATGCTGTTCAAGCGCGCTAAAGAGCTCAGAACAGG
CCTAAAGCAGATTGGCTTTCATATCCGCAGCGAATCGCAAATTGTGGCGTTAGAATGCGGTAGTGAGCGAAATACGGAAC
GAGTGCGTGACTTCTTAGAAGAACGAAACGTGTTTGGCGCTGTATTCTGTCGTCCTGCAACGGGCAAGAATAAGAACATT
ATTCGATTTTCGATCAATGCTGATATGACATCTCGTGACATTGACCATGTCTTGACGGCTTGTCAGGAAGCGTATAACCA
CCCAGAACTAGAATTCGCCTAA

Upstream 100 bases:

>100_bases
TTCATAAATATTTATTATGTAATTTGACGATTATTTTTTAAGCACGATCTTAGTTAGACCCCCGAACGGTAAGGAGTTAC
AAAGCTCACCCAACCCTTAA

Downstream 100 bases:

>100_bases
CGAATCGAAAGCGGCACATTTGAATGGAAGCTTGTGAATGCCGGAATACAAAGAGCTCAGCATAGTGCTGAGCTCTATTC
GTTTTCGTCACCCTATTGAG

Product: hypothetical protein

Products: Acetyl-CoA; Glycine [C]

Alternate protein names: Cholerae quorum-sensing autoinducer [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MRSLQAHANLCNLTNDTELILVKLYNCQRWRFPVLRRELIMSDKPKTKPLPSFVEGRLDFYIQDLIEQNENQKHLVLGKR
PQQGAVVMQSNDYLSLSHNLQIQQAHRDAIYEHDDNVVMSAIFLQDDDSKPAFETQLAEYVGMGSCLLSQSGWAANIGLL
QTICPPETPVYIDFFAHMSLWEGIRAAGAQAHPFMHNNMNHLRKQIQRNGSGVIVVDSVYSTIGTIAPLRDIYEMAREFD
CALVVDESHSLGTHGPNGSGLVKALELTEQVDFITVSLAKTFAYRAGAILGPEKLARTLPFVAFPAIFSSTVLPQEIVRL
EKTLEVIRSADDKRTMLFKRAKELRTGLKQIGFHIRSESQIVALECGSERNTERVRDFLEERNVFGAVFCRPATGKNKNI
IRFSINADMTSRDIDHVLTACQEAYNHPELEFA

Sequences:

>Translated_433_residues
MRSLQAHANLCNLTNDTELILVKLYNCQRWRFPVLRRELIMSDKPKTKPLPSFVEGRLDFYIQDLIEQNENQKHLVLGKR
PQQGAVVMQSNDYLSLSHNLQIQQAHRDAIYEHDDNVVMSAIFLQDDDSKPAFETQLAEYVGMGSCLLSQSGWAANIGLL
QTICPPETPVYIDFFAHMSLWEGIRAAGAQAHPFMHNNMNHLRKQIQRNGSGVIVVDSVYSTIGTIAPLRDIYEMAREFD
CALVVDESHSLGTHGPNGSGLVKALELTEQVDFITVSLAKTFAYRAGAILGPEKLARTLPFVAFPAIFSSTVLPQEIVRL
EKTLEVIRSADDKRTMLFKRAKELRTGLKQIGFHIRSESQIVALECGSERNTERVRDFLEERNVFGAVFCRPATGKNKNI
IRFSINADMTSRDIDHVLTACQEAYNHPELEFA
>Mature_433_residues
MRSLQAHANLCNLTNDTELILVKLYNCQRWRFPVLRRELIMSDKPKTKPLPSFVEGRLDFYIQDLIEQNENQKHLVLGKR
PQQGAVVMQSNDYLSLSHNLQIQQAHRDAIYEHDDNVVMSAIFLQDDDSKPAFETQLAEYVGMGSCLLSQSGWAANIGLL
QTICPPETPVYIDFFAHMSLWEGIRAAGAQAHPFMHNNMNHLRKQIQRNGSGVIVVDSVYSTIGTIAPLRDIYEMAREFD
CALVVDESHSLGTHGPNGSGLVKALELTEQVDFITVSLAKTFAYRAGAILGPEKLARTLPFVAFPAIFSSTVLPQEIVRL
EKTLEVIRSADDKRTMLFKRAKELRTGLKQIGFHIRSESQIVALECGSERNTERVRDFLEERNVFGAVFCRPATGKNKNI
IRFSINADMTSRDIDHVLTACQEAYNHPELEFA

Specific function: Required for the synthesis of the quorum-sensing autoinducer CAI-1 ((S)-3-hydroxytridecan-4-one) which probably functions as an intragenus signal [H]

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI284448556, Length=341, Percent_Identity=26.9794721407625, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI7657118, Length=341, Percent_Identity=26.9794721407625, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI4502025, Length=344, Percent_Identity=25, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI40316939, Length=344, Percent_Identity=25, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI83977442, Length=330, Percent_Identity=25.1515151515152, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI83977440, Length=330, Percent_Identity=25.1515151515152, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI83977444, Length=330, Percent_Identity=25.1515151515152, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI4758668, Length=373, Percent_Identity=24.1286863270777, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI119220554, Length=327, Percent_Identity=24.4648318042813, Blast_Score=90, Evalue=4e-18,
Organism=Escherichia coli, GI1790046, Length=338, Percent_Identity=25.1479289940828, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1786993, Length=334, Percent_Identity=26.9461077844311, Blast_Score=112, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI71994529, Length=338, Percent_Identity=23.6686390532544, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI32566772, Length=358, Percent_Identity=22.3463687150838, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17560912, Length=318, Percent_Identity=25.4716981132075, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17560914, Length=318, Percent_Identity=25.4716981132075, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71982625, Length=267, Percent_Identity=22.8464419475655, Blast_Score=72, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI71982617, Length=267, Percent_Identity=22.8464419475655, Blast_Score=71, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6320267, Length=312, Percent_Identity=24.0384615384615, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6320438, Length=381, Percent_Identity=22.5721784776903, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6323954, Length=271, Percent_Identity=20.2952029520295, Blast_Score=63, Evalue=1e-10,
Organism=Drosophila melanogaster, GI24662918, Length=335, Percent_Identity=25.9701492537313, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI17137420, Length=316, Percent_Identity=25.3164556962025, Blast_Score=112, Evalue=7e-25,
Organism=Drosophila melanogaster, GI17136286, Length=332, Percent_Identity=24.0963855421687, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004839
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: 2.3.1.29 [C]

Molecular weight: Translated: 48848; Mature: 48848

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSLQAHANLCNLTNDTELILVKLYNCQRWRFPVLRRELIMSDKPKTKPLPSFVEGRLDF
CCCCHHHCCHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHH
YIQDLIEQNENQKHLVLGKRPQQGAVVMQSNDYLSLSHNLQIQQAHRDAIYEHDDNVVMS
HHHHHHHCCCCCCEEEECCCCCCCEEEEECCCEEEEECCCEEEHHHHHHHHCCCCCEEEE
AIFLQDDDSKPAFETQLAEYVGMGSCLLSQSGWAANIGLLQTICPPETPVYIDFFAHMSL
EEEEECCCCCCHHHHHHHHHHCCCHHHHCCCCCEECCCHHHHCCCCCCCEEEHHHHHHHH
WEGIRAAGAQAHPFMHNNMNHLRKQIQRNGSGVIVVDSVYSTIGTIAPLRDIYEMAREFD
HHHHHHCCCCCCCCHHCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCC
CALVVDESHSLGTHGPNGSGLVKALELTEQVDFITVSLAKTFAYRAGAILGPEKLARTLP
EEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHEEHHHHHHHHHHCCCCCCHHHHHHHCC
FVAFPAIFSSTVLPQEIVRLEKTLEVIRSADDKRTMLFKRAKELRTGLKQIGFHIRSESQ
HHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEECCCCC
IVALECGSERNTERVRDFLEERNVFGAVFCRPATGKNKNIIRFSINADMTSRDIDHVLTA
EEEEECCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHH
CQEAYNHPELEFA
HHHHHCCCCCCCC
>Mature Secondary Structure
MRSLQAHANLCNLTNDTELILVKLYNCQRWRFPVLRRELIMSDKPKTKPLPSFVEGRLDF
CCCCHHHCCHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHH
YIQDLIEQNENQKHLVLGKRPQQGAVVMQSNDYLSLSHNLQIQQAHRDAIYEHDDNVVMS
HHHHHHHCCCCCCEEEECCCCCCCEEEEECCCEEEEECCCEEEHHHHHHHHCCCCCEEEE
AIFLQDDDSKPAFETQLAEYVGMGSCLLSQSGWAANIGLLQTICPPETPVYIDFFAHMSL
EEEEECCCCCCHHHHHHHHHHCCCHHHHCCCCCEECCCHHHHCCCCCCCEEEHHHHHHHH
WEGIRAAGAQAHPFMHNNMNHLRKQIQRNGSGVIVVDSVYSTIGTIAPLRDIYEMAREFD
HHHHHHCCCCCCCCHHCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCC
CALVVDESHSLGTHGPNGSGLVKALELTEQVDFITVSLAKTFAYRAGAILGPEKLARTLP
EEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHEEHHHHHHHHHHCCCCCCHHHHHHHCC
FVAFPAIFSSTVLPQEIVRLEKTLEVIRSADDKRTMLFKRAKELRTGLKQIGFHIRSESQ
HHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEECCCCC
IVALECGSERNTERVRDFLEERNVFGAVFCRPATGKNKNIIRFSINADMTSRDIDHVLTA
EEEEECCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHH
CQEAYNHPELEFA
HHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal 5-phosphate mol per mol subunit, enzyme, [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.08 {propionyl-CoA}} 0.059 {acetyl-CoA}} 12 {butyryl-CoA}} [C]

Substrates: L-2-Amino-3-oxobutanoate; Coenzyme A [C]

Specific reaction: L-2-Amino-3-oxobutanoate + Coenzyme A --> Acetyl-CoA + Glycine [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 2-Cyclohexanedione; 2, 3-Butanedione; 4-(Oxoacetyl) phenoxyacetic acid; Aminomalonic acid; Aminomethyl phosphonic acid; Aminooxyacetate; Ba2+; Ca2+; Cd2+; CoA; Cu2+; DTT; Glutathione; Hg2+; Hydroxy l amine; IAA; L-Cysteine; Mn2+; PCMB, NEM, glycinemeth

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA