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The map label for this gene is glyA2 [H]

Identifier: 156977185

GI number: 156977185

Start: 1159954

End: 1161249

Strand: Direct

Name: glyA2 [H]

Synonym: VIBHAR_05971

Alternate gene names: 156977185

Gene position: 1159954-1161249 (Clockwise)

Preceding gene: 156977180

Following gene: 156977186

Centisome position: 52.63

GC content: 48.61

Gene sequence:

>1296_bases
ATGAACAAGAGTTACCCTAACCACAGCTTGGAAAACTTTTTCTCTACCAACCTCTCTGCGACAGATGACGCTGTCTTTGC
TGGAATTCAGGCAGAATTCGCTCGCCAAAACGAACAAATCGAACTTATCGCTTCTGAGAACATCGTTTCTAAAGCGGTAA
TGCAAGCTCAAGGCACATGCCTAACAAACAAATACGCTGAAGGTTACCCAGGTCGCCGTTACTATGGTGGTTGTGAGCAC
GTAGATACAGTGGAAGCCATTGCTATCGAACGCGCGAAAAAACTTTTCAATTGTGAATACGCAAACGTTCAACCACACTC
AGGCGCACAAGCAAACGGCGCAGTAAAACTGGCACTGCTGCAACCTGGCGACACCATTCTTGGTATGTCTCTAGACGCAG
GTGGCCACCTAACTCACGGTGCTCGCCCTGCACTTTCTGGTAAATGGTTTAACGCAGTGCAATACGGCGTTGACCGCGAA
ACGCTAGAAATCAACTACGACGACGTTCGTGAGCTAGCACTTGAGCACCAACCTAAAATGATCATCGCAGGTGGTAGTGC
AATTCCACGTACTATCGACTTCGCAAAATTCCGTGAAATCGCAGACGAAGTAAACGCGATTCTTATGGTCGACATGGCGC
ACATCTCCGGTCTTATCGCAACAGGCGCTCACCCTAGCCCGCTTCCTCACGCACACGTTGTTACTACGACAACGCACAAA
ACATTGCGTGGCCCACGTGGCGGTATGATCCTGACTAACCACGAAGACATCATTAAGAAAATCAACTCTGCGGTATTCCC
AGGTTTACAAGGCGGCCCACTGATGCACGTGATTGCCTCTAAAGCAGTGGCATTCGGTGAAGCCCTAGGTCCTGAATTCA
AAACTTATATCGATTCGGTGATCAACAACGCAAAAGTCATGGCAGAAGTATTGCAAACTCGCGGTTGCGACATCGTAACC
GGTGGTACTGACACGCACCTAATGCTGGTTGACCTTCGCCCTAAAGGTTTGAAAGGTAACAAAGCAGAAGAAGCACTAGA
GCGTGCAGGCATCACATGTAACAAAAATGGCATCCCATTCGACACAGAGAAGCCTATGATTACATCGGGCATCCGTTTAG
GTACGCCTGCGGGTACAAGCCGCGGCTTTGGCGCTGAAGAATTCAAACTCATTGGTAATTGGATTGGTGACGTACTGGAT
GGTTTGGTTAGCAACCCAGAAGGTGACGCAGAAGTAGAAAAACGCGTACGCAAGCAAGTTAAAGAGCTTTGCAGTCGCTT
CCCTCTTTACCAGTAA

Upstream 100 bases:

>100_bases
GCTATGCAGTTCAGCTGTTTTGAGGACAAATGAACCACCATCGCAATCAAACAACTATTTCTACTGCGGGTTCATTTTCA
GATTTGGAAGGTCAACTACC

Downstream 100 bases:

>100_bases
ACGATTTTTTATATCAGAATAAATCCTGACAAGTTATTTGGAGATTAAGCAATGGACAAAACACTGAAGTTTACAGATAG
CCACGAGTGGGTACGTGACA

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT 2; Serine methylase 2 [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MNKSYPNHSLENFFSTNLSATDDAVFAGIQAEFARQNEQIELIASENIVSKAVMQAQGTCLTNKYAEGYPGRRYYGGCEH
VDTVEAIAIERAKKLFNCEYANVQPHSGAQANGAVKLALLQPGDTILGMSLDAGGHLTHGARPALSGKWFNAVQYGVDRE
TLEINYDDVRELALEHQPKMIIAGGSAIPRTIDFAKFREIADEVNAILMVDMAHISGLIATGAHPSPLPHAHVVTTTTHK
TLRGPRGGMILTNHEDIIKKINSAVFPGLQGGPLMHVIASKAVAFGEALGPEFKTYIDSVINNAKVMAEVLQTRGCDIVT
GGTDTHLMLVDLRPKGLKGNKAEEALERAGITCNKNGIPFDTEKPMITSGIRLGTPAGTSRGFGAEEFKLIGNWIGDVLD
GLVSNPEGDAEVEKRVRKQVKELCSRFPLYQ

Sequences:

>Translated_431_residues
MNKSYPNHSLENFFSTNLSATDDAVFAGIQAEFARQNEQIELIASENIVSKAVMQAQGTCLTNKYAEGYPGRRYYGGCEH
VDTVEAIAIERAKKLFNCEYANVQPHSGAQANGAVKLALLQPGDTILGMSLDAGGHLTHGARPALSGKWFNAVQYGVDRE
TLEINYDDVRELALEHQPKMIIAGGSAIPRTIDFAKFREIADEVNAILMVDMAHISGLIATGAHPSPLPHAHVVTTTTHK
TLRGPRGGMILTNHEDIIKKINSAVFPGLQGGPLMHVIASKAVAFGEALGPEFKTYIDSVINNAKVMAEVLQTRGCDIVT
GGTDTHLMLVDLRPKGLKGNKAEEALERAGITCNKNGIPFDTEKPMITSGIRLGTPAGTSRGFGAEEFKLIGNWIGDVLD
GLVSNPEGDAEVEKRVRKQVKELCSRFPLYQ
>Mature_431_residues
MNKSYPNHSLENFFSTNLSATDDAVFAGIQAEFARQNEQIELIASENIVSKAVMQAQGTCLTNKYAEGYPGRRYYGGCEH
VDTVEAIAIERAKKLFNCEYANVQPHSGAQANGAVKLALLQPGDTILGMSLDAGGHLTHGARPALSGKWFNAVQYGVDRE
TLEINYDDVRELALEHQPKMIIAGGSAIPRTIDFAKFREIADEVNAILMVDMAHISGLIATGAHPSPLPHAHVVTTTTHK
TLRGPRGGMILTNHEDIIKKINSAVFPGLQGGPLMHVIASKAVAFGEALGPEFKTYIDSVINNAKVMAEVLQTRGCDIVT
GGTDTHLMLVDLRPKGLKGNKAEEALERAGITCNKNGIPFDTEKPMITSGIRLGTPAGTSRGFGAEEFKLIGNWIGDVLD
GLVSNPEGDAEVEKRVRKQVKELCSRFPLYQ

Specific function: Interconversion of serine and glycine [H]

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family [H]

Homologues:

Organism=Homo sapiens, GI261862352, Length=452, Percent_Identity=44.2477876106195, Blast_Score=348, Evalue=4e-96,
Organism=Homo sapiens, GI261862350, Length=452, Percent_Identity=44.2477876106195, Blast_Score=348, Evalue=4e-96,
Organism=Homo sapiens, GI261862348, Length=452, Percent_Identity=44.2477876106195, Blast_Score=348, Evalue=4e-96,
Organism=Homo sapiens, GI19923315, Length=452, Percent_Identity=44.2477876106195, Blast_Score=348, Evalue=5e-96,
Organism=Homo sapiens, GI261862346, Length=455, Percent_Identity=43.7362637362637, Blast_Score=337, Evalue=1e-92,
Organism=Homo sapiens, GI22547186, Length=413, Percent_Identity=47.2154963680387, Blast_Score=328, Evalue=8e-90,
Organism=Homo sapiens, GI22547189, Length=396, Percent_Identity=44.4444444444444, Blast_Score=293, Evalue=2e-79,
Organism=Escherichia coli, GI1788902, Length=416, Percent_Identity=58.4134615384615, Blast_Score=462, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI25144732, Length=458, Percent_Identity=43.8864628820961, Blast_Score=344, Evalue=4e-95,
Organism=Caenorhabditis elegans, GI25144729, Length=458, Percent_Identity=43.8864628820961, Blast_Score=344, Evalue=6e-95,
Organism=Saccharomyces cerevisiae, GI6319739, Length=441, Percent_Identity=42.4036281179138, Blast_Score=330, Evalue=3e-91,
Organism=Saccharomyces cerevisiae, GI6323087, Length=411, Percent_Identity=43.7956204379562, Blast_Score=323, Evalue=2e-89,
Organism=Drosophila melanogaster, GI24640005, Length=464, Percent_Identity=43.75, Blast_Score=359, Evalue=2e-99,
Organism=Drosophila melanogaster, GI221329721, Length=460, Percent_Identity=43.9130434782609, Blast_Score=357, Evalue=7e-99,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798 [H]

Pfam domain/function: PF00464 SHMT [H]

EC number: =2.1.2.1 [H]

Molecular weight: Translated: 46574; Mature: 46574

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKSYPNHSLENFFSTNLSATDDAVFAGIQAEFARQNEQIELIASENIVSKAVMQAQGTC
CCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHCCCE
LTNKYAEGYPGRRYYGGCEHVDTVEAIAIERAKKLFNCEYANVQPHSGAQANGAVKLALL
EECHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEE
QPGDTILGMSLDAGGHLTHGARPALSGKWFNAVQYGVDRETLEINYDDVRELALEHQPKM
CCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEECHHHHHHHHHHCCCCE
IIAGGSAIPRTIDFAKFREIADEVNAILMVDMAHISGLIATGAHPSPLPHAHVVTTTTHK
EEECCCCCCCHHHHHHHHHHHHHHCEEEEEEHHHHHHHHCCCCCCCCCCCCEEEEECCHH
TLRGPRGGMILTNHEDIIKKINSAVFPGLQGGPLMHVIASKAVAFGEALGPEFKTYIDSV
HHCCCCCCEEEECHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHH
INNAKVMAEVLQTRGCDIVTGGTDTHLMLVDLRPKGLKGNKAEEALERAGITCNKNGIPF
HHHHHHHHHHHHHCCCEEEECCCCCEEEEEEECCCCCCCCHHHHHHHHCCCEECCCCCCC
DTEKPMITSGIRLGTPAGTSRGFGAEEFKLIGNWIGDVLDGLVSNPEGDAEVEKRVRKQV
CCCCCHHHCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
KELCSRFPLYQ
HHHHHHCCCCC
>Mature Secondary Structure
MNKSYPNHSLENFFSTNLSATDDAVFAGIQAEFARQNEQIELIASENIVSKAVMQAQGTC
CCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHCCCE
LTNKYAEGYPGRRYYGGCEHVDTVEAIAIERAKKLFNCEYANVQPHSGAQANGAVKLALL
EECHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEE
QPGDTILGMSLDAGGHLTHGARPALSGKWFNAVQYGVDRETLEINYDDVRELALEHQPKM
CCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEECHHHHHHHHHHCCCCE
IIAGGSAIPRTIDFAKFREIADEVNAILMVDMAHISGLIATGAHPSPLPHAHVVTTTTHK
EEECCCCCCCHHHHHHHHHHHHHHCEEEEEEHHHHHHHHCCCCCCCCCCCCEEEEECCHH
TLRGPRGGMILTNHEDIIKKINSAVFPGLQGGPLMHVIASKAVAFGEALGPEFKTYIDSV
HHCCCCCCEEEECHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHH
INNAKVMAEVLQTRGCDIVTGGTDTHLMLVDLRPKGLKGNKAEEALERAGITCNKNGIPF
HHHHHHHHHHHHHCCCEEEECCCCCEEEEEEECCCCCCCCHHHHHHHHCCCEECCCCCCC
DTEKPMITSGIRLGTPAGTSRGFGAEEFKLIGNWIGDVLDGLVSNPEGDAEVEKRVRKQV
CCCCCHHHCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
KELCSRFPLYQ
HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]