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The map label for this gene is gcvH

Identifier: 156977186

GI number: 156977186

Start: 1161301

End: 1161681

Strand: Direct

Name: gcvH

Synonym: VIBHAR_05972

Alternate gene names: 156977186

Gene position: 1161301-1161681 (Clockwise)

Preceding gene: 156977185

Following gene: 156977187

Centisome position: 52.69

GC content: 45.14

Gene sequence:

>381_bases
ATGGACAAAACACTGAAGTTTACAGATAGCCACGAGTGGGTACGTGACAACGGTGACGGCACAGCAACTATCGGTATTTC
TGAGCACGCTCAAGAAATGCTAGGTGACGTGGTATTTGTTGACCTACCAGACGTGGAAGATGAAGTCGAAGCAGGCGAAA
GCTTTTCTTTGGTGGAGTCTGTAAAAGCGGCTTCAGACATCTACTCACCAATCACTGGCGAAGTAGTTGAAATCAACGAA
GAACTAGAAGACAGCCCAGAGCTAATCAACGAAGAACCATACGAAGGTGGTTGGATTGTGAAAGTGAAGCTTTCAGACCC
ATCTGAGTTGGATGACTTAAAAGATGCGGAAGAATACCTAAGCTCTATCGAAGAAGAGTAA

Upstream 100 bases:

>100_bases
ACGCAAGCAAGTTAAAGAGCTTTGCAGTCGCTTCCCTCTTTACCAGTAAACGATTTTTTATATCAGAATAAATCCTGACA
AGTTATTTGGAGATTAAGCA

Downstream 100 bases:

>100_bases
TTAGGATGAGACGAAAGCTGTCCCCTAGCATAACGATTAGTGGACAGCTTTTTTTCTAGGTTCGGACGTATAATTTAGAT
TATCAGTAGCGTAAATCCGT

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 126; Mature: 126

Protein sequence:

>126_residues
MDKTLKFTDSHEWVRDNGDGTATIGISEHAQEMLGDVVFVDLPDVEDEVEAGESFSLVESVKAASDIYSPITGEVVEINE
ELEDSPELINEEPYEGGWIVKVKLSDPSELDDLKDAEEYLSSIEEE

Sequences:

>Translated_126_residues
MDKTLKFTDSHEWVRDNGDGTATIGISEHAQEMLGDVVFVDLPDVEDEVEAGESFSLVESVKAASDIYSPITGEVVEINE
ELEDSPELINEEPYEGGWIVKVKLSDPSELDDLKDAEEYLSSIEEE
>Mature_126_residues
MDKTLKFTDSHEWVRDNGDGTATIGISEHAQEMLGDVVFVDLPDVEDEVEAGESFSLVESVKAASDIYSPITGEVVEINE
ELEDSPELINEEPYEGGWIVKVKLSDPSELDDLKDAEEYLSSIEEE

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=123, Percent_Identity=52.0325203252033, Blast_Score=128, Evalue=1e-30,
Organism=Homo sapiens, GI89057342, Length=123, Percent_Identity=51.219512195122, Blast_Score=125, Evalue=1e-29,
Organism=Escherichia coli, GI1789271, Length=122, Percent_Identity=59.0163934426229, Blast_Score=155, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17507493, Length=114, Percent_Identity=50, Blast_Score=111, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17551294, Length=113, Percent_Identity=45.1327433628319, Blast_Score=110, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6319272, Length=120, Percent_Identity=41.6666666666667, Blast_Score=104, Evalue=5e-24,
Organism=Drosophila melanogaster, GI17865652, Length=119, Percent_Identity=45.3781512605042, Blast_Score=115, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_VIBHB (A7N5C3)

Other databases:

- EMBL:   CP000790
- RefSeq:   YP_001448092.1
- ProteinModelPortal:   A7N5C3
- SMR:   A7N5C3
- STRING:   A7N5C3
- GeneID:   5558847
- GenomeReviews:   CP000790_GR
- KEGG:   vha:VIBHAR_05972
- NMPDR:   fig|338187.4.peg.3530
- eggNOG:   COG0509
- HOGENOM:   HBG693789
- OMA:   FTDSHEW
- ProtClustDB:   PRK01202
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 13980; Mature: 13980

Theoretical pI: Translated: 3.56; Mature: 3.56

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKTLKFTDSHEWVRDNGDGTATIGISEHAQEMLGDVVFVDLPDVEDEVEAGESFSLVES
CCCCCCCCCCCCCEEECCCCEEEECHHHHHHHHHCCEEEEECCCCCHHHHCCCCHHHHHH
VKAASDIYSPITGEVVEINEELEDSPELINEEPYEGGWIVKVKLSDPSELDDLKDAEEYL
HHHHHHHCCCCCCCEEEECHHCCCCCHHCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHH
SSIEEE
HHHCCC
>Mature Secondary Structure
MDKTLKFTDSHEWVRDNGDGTATIGISEHAQEMLGDVVFVDLPDVEDEVEAGESFSLVES
CCCCCCCCCCCCCEEECCCCEEEECHHHHHHHHHCCEEEEECCCCCHHHHCCCCHHHHHH
VKAASDIYSPITGEVVEINEELEDSPELINEEPYEGGWIVKVKLSDPSELDDLKDAEEYL
HHHHHHHCCCCCCCEEEECHHCCCCCHHCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHH
SSIEEE
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA