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The map label for this gene is pntA [H]

Identifier: 156976948

GI number: 156976948

Start: 934930

End: 936486

Strand: Direct

Name: pntA [H]

Synonym: VIBHAR_05733

Alternate gene names: 156976948

Gene position: 934930-936486 (Clockwise)

Preceding gene: 156976947

Following gene: 156976949

Centisome position: 42.42

GC content: 48.43

Gene sequence:

>1557_bases
TTGCAAATCGGTGTACCTAAGGAAATACTCGCAGGTGAATCGCGAGTAGCTGCCTCACCTAAATCGGTTGAGCAGTTAAT
TAAGTTGGGGTTCGATGTCGCAATCGAATCACAAGCGGGGGTGCTAGCCAGTTTCGATGATGCCGCTTATGAAGCAGCAG
GGGCGAAAATTGTATCTAGTGAAGAAGTATGGACGGCAGGTTTAATCCTTAAAGTGAACGCGCCACTCGTAGATGAAGAA
AAAGGCATCGACGAAATCGCATTACTGCAAGATGGCGCAACATTAGTGAGCTTTATCTGGCCAGCTCAAAATGCAGAGTT
AATGGGACAACTCTCTAGCAAGAACATCAACGTTCTCGCAATGGACGCAGTTCCTCGTATCTCTCGTGCGCAAGCATTAG
ACGCACTCTCTTCTATGGCGAACATTGCCGGTTACCGCGCAGTGGTTGAAGCCGCTCATGAATTCGGTCGATTCTTCACC
GGTCAAATTACCGCAGCAGGTAAAGTGCCGCCAGCGAAAGTACTGGTTGCAGGTGCAGGTGTTGCAGGTCTTGCTGCTAT
CGGCGCTGCTGGTAGCCTTGGCGCTATCGTTCGCTCTTTTGACGTTCGTCCTGAAGTAAAAGAGCAAGTTGAATCGATGG
GCGCAGAGTTCCTAACCGTAGACTTCAAAGAAGATTCCGGTTCAGGTGACGGCTACGCGAAAGAAATGTCGGACGAATTC
AACAAAAAAGCCGCTGAGCTATACGCTGAGCAAGCGAAAGACGTTGATATCATCGTCACAACGGCTCTGATTCCTGGTAA
ACCTGCGCCAACGCTTATCACCAAAGAAATGGTGGATAGCATGAAAGCGGGCAGCGTAATCGTCGACCTTGCTGCCGCAA
ACGGTGGGAACTGCGAATACACAGTAAAAGATCAAGTTATTACCACGGCTAACGGCGTGAAAATCGTTGGTTACACTGAC
ATGGTAGGTCGCCTGCCTACGCAGTCTTCTCAGCTTTACGCAACCAACCTTGTAAACCTTCTAAAACTGCTTTGCAAAGA
GAAAGATGGCAACATCGATATCGACTTCGAAGACGTTGTACTTCGTGGCGTAACAGTAGTAAAAGAAGGCGAAGTGACAT
GGCCTGCGCCACCAATTCAGGTGTCGGCACAGCCACAAGCTAAACCGCAAGAACCCGTTAAGCCTCAGCCTAAAGTTGAA
AAGCCAACGTCACCAGTGAAGAAATTCGGTGCACTCGCTGTCGGTTTAGGTGCCTTTGCTTGGGTTGCTTCTGTTGCTCC
GGCTGCGTTCTTATCTCACTTCACCGTATTCGTACTGGCGTGTGTGGTAGGTTACTACGTAGTTTGGAACGTAACACATG
CACTGCATACTCCACTTATGTCTGTAACCAACGCGATTTCAGGCATCATCGTAGTAGGTGCACTGCTGCAAATTGGTCAG
GGTAATGGCGTCGTTACCTTCCTATCTTTCATTGCTGTACTCATTGCAAGCATAAACATCTTTGGTGGCTTTACCGTGAC
CAAACGTATGCTTGAAATGTTCCGCAAAGATAAATAA

Upstream 100 bases:

>100_bases
TTTTCTTGCGTAGTAAACAGATAGAAATAAAAAAGAATTAGAGTAGAGCAACGCTCTGTTTTGGGACTAATTATTGATAT
GTATACAAGGAGTATGCGTT

Downstream 100 bases:

>100_bases
TAGGAGTGACCAATGTCTGCAGGATTAGTACAAGCGGCATACATTATTGCTGCGCTATTTTTCATATTAAGTCTCGCGGG
ACTTTCTAAACAAGAATCAG

Product: NAD(P) transhydrogenase subunit alpha

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit alpha; Pyridine nucleotide transhydrogenase subunit alpha [H]

Number of amino acids: Translated: 518; Mature: 518

Protein sequence:

>518_residues
MQIGVPKEILAGESRVAASPKSVEQLIKLGFDVAIESQAGVLASFDDAAYEAAGAKIVSSEEVWTAGLILKVNAPLVDEE
KGIDEIALLQDGATLVSFIWPAQNAELMGQLSSKNINVLAMDAVPRISRAQALDALSSMANIAGYRAVVEAAHEFGRFFT
GQITAAGKVPPAKVLVAGAGVAGLAAIGAAGSLGAIVRSFDVRPEVKEQVESMGAEFLTVDFKEDSGSGDGYAKEMSDEF
NKKAAELYAEQAKDVDIIVTTALIPGKPAPTLITKEMVDSMKAGSVIVDLAAANGGNCEYTVKDQVITTANGVKIVGYTD
MVGRLPTQSSQLYATNLVNLLKLLCKEKDGNIDIDFEDVVLRGVTVVKEGEVTWPAPPIQVSAQPQAKPQEPVKPQPKVE
KPTSPVKKFGALAVGLGAFAWVASVAPAAFLSHFTVFVLACVVGYYVVWNVTHALHTPLMSVTNAISGIIVVGALLQIGQ
GNGVVTFLSFIAVLIASINIFGGFTVTKRMLEMFRKDK

Sequences:

>Translated_518_residues
MQIGVPKEILAGESRVAASPKSVEQLIKLGFDVAIESQAGVLASFDDAAYEAAGAKIVSSEEVWTAGLILKVNAPLVDEE
KGIDEIALLQDGATLVSFIWPAQNAELMGQLSSKNINVLAMDAVPRISRAQALDALSSMANIAGYRAVVEAAHEFGRFFT
GQITAAGKVPPAKVLVAGAGVAGLAAIGAAGSLGAIVRSFDVRPEVKEQVESMGAEFLTVDFKEDSGSGDGYAKEMSDEF
NKKAAELYAEQAKDVDIIVTTALIPGKPAPTLITKEMVDSMKAGSVIVDLAAANGGNCEYTVKDQVITTANGVKIVGYTD
MVGRLPTQSSQLYATNLVNLLKLLCKEKDGNIDIDFEDVVLRGVTVVKEGEVTWPAPPIQVSAQPQAKPQEPVKPQPKVE
KPTSPVKKFGALAVGLGAFAWVASVAPAAFLSHFTVFVLACVVGYYVVWNVTHALHTPLMSVTNAISGIIVVGALLQIGQ
GNGVVTFLSFIAVLIASINIFGGFTVTKRMLEMFRKDK
>Mature_518_residues
MQIGVPKEILAGESRVAASPKSVEQLIKLGFDVAIESQAGVLASFDDAAYEAAGAKIVSSEEVWTAGLILKVNAPLVDEE
KGIDEIALLQDGATLVSFIWPAQNAELMGQLSSKNINVLAMDAVPRISRAQALDALSSMANIAGYRAVVEAAHEFGRFFT
GQITAAGKVPPAKVLVAGAGVAGLAAIGAAGSLGAIVRSFDVRPEVKEQVESMGAEFLTVDFKEDSGSGDGYAKEMSDEF
NKKAAELYAEQAKDVDIIVTTALIPGKPAPTLITKEMVDSMKAGSVIVDLAAANGGNCEYTVKDQVITTANGVKIVGYTD
MVGRLPTQSSQLYATNLVNLLKLLCKEKDGNIDIDFEDVVLRGVTVVKEGEVTWPAPPIQVSAQPQAKPQEPVKPQPKVE
KPTSPVKKFGALAVGLGAFAWVASVAPAAFLSHFTVFVLACVVGYYVVWNVTHALHTPLMSVTNAISGIIVVGALLQIGQ
GNGVVTFLSFIAVLIASINIFGGFTVTKRMLEMFRKDK

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG3288

COG function: function code C; NAD/NADP transhydrogenase alpha subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=544, Percent_Identity=49.6323529411765, Blast_Score=474, Evalue=1e-134,
Organism=Homo sapiens, GI122939153, Length=544, Percent_Identity=49.6323529411765, Blast_Score=474, Evalue=1e-134,
Organism=Escherichia coli, GI1787887, Length=517, Percent_Identity=74.8549323017408, Blast_Score=773, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17550456, Length=528, Percent_Identity=51.8939393939394, Blast_Score=475, Evalue=1e-134,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR004571 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 54549; Mature: 54549

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00836 ALADH_PNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIGVPKEILAGESRVAASPKSVEQLIKLGFDVAIESQAGVLASFDDAAYEAAGAKIVSS
CCCCCCHHHHCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCEEECCHHHHHHCCCEEECC
EEVWTAGLILKVNAPLVDEEKGIDEIALLQDGATLVSFIWPAQNAELMGQLSSKNINVLA
CCEEEEEEEEEECCCCCCCCCCCCCEEEECCCCCEEHEECCCCCHHHHHHHCCCCCCEEE
MDAVPRISRAQALDALSSMANIAGYRAVVEAAHEFGRFFTGQITAAGKVPPAKVLVAGAG
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECEEEECCCCCCCCEEEECCC
VAGLAAIGAAGSLGAIVRSFDVRPEVKEQVESMGAEFLTVDFKEDSGSGDGYAKEMSDEF
HHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHH
NKKAAELYAEQAKDVDIIVTTALIPGKPAPTLITKEMVDSMKAGSVIVDLAAANGGNCEY
HHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCEE
TVKDQVITTANGVKIVGYTDMVGRLPTQSSQLYATNLVNLLKLLCKEKDGNIDIDFEDVV
EECCCEEEECCCEEEEEEHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEECCHHHHH
LRGVTVVKEGEVTWPAPPIQVSAQPQAKPQEPVKPQPKVEKPTSPVKKFGALAVGLGAFA
HHCEEEEECCCEECCCCCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
WVASVAPAAFLSHFTVFVLACVVGYYVVWNVTHALHTPLMSVTNAISGIIVVGALLQIGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GNGVVTFLSFIAVLIASINIFGGFTVTKRMLEMFRKDK
CCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQIGVPKEILAGESRVAASPKSVEQLIKLGFDVAIESQAGVLASFDDAAYEAAGAKIVSS
CCCCCCHHHHCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCEEECCHHHHHHCCCEEECC
EEVWTAGLILKVNAPLVDEEKGIDEIALLQDGATLVSFIWPAQNAELMGQLSSKNINVLA
CCEEEEEEEEEECCCCCCCCCCCCCEEEECCCCCEEHEECCCCCHHHHHHHCCCCCCEEE
MDAVPRISRAQALDALSSMANIAGYRAVVEAAHEFGRFFTGQITAAGKVPPAKVLVAGAG
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECEEEECCCCCCCCEEEECCC
VAGLAAIGAAGSLGAIVRSFDVRPEVKEQVESMGAEFLTVDFKEDSGSGDGYAKEMSDEF
HHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHH
NKKAAELYAEQAKDVDIIVTTALIPGKPAPTLITKEMVDSMKAGSVIVDLAAANGGNCEY
HHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCEE
TVKDQVITTANGVKIVGYTDMVGRLPTQSSQLYATNLVNLLKLLCKEKDGNIDIDFEDVV
EECCCEEEECCCEEEEEEHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEECCHHHHH
LRGVTVVKEGEVTWPAPPIQVSAQPQAKPQEPVKPQPKVEKPTSPVKKFGALAVGLGAFA
HHCEEEEECCCEECCCCCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
WVASVAPAAFLSHFTVFVLACVVGYYVVWNVTHALHTPLMSVTNAISGIIVVGALLQIGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GNGVVTFLSFIAVLIASINIFGGFTVTKRMLEMFRKDK
CCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3525165; 1633824; 9097039; 9278503; 1932078 [H]