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The map label for this gene is amyS [H]

Identifier: 156976898

GI number: 156976898

Start: 887551

End: 889074

Strand: Direct

Name: amyS [H]

Synonym: VIBHAR_05679

Alternate gene names: 156976898

Gene position: 887551-889074 (Clockwise)

Preceding gene: 156976897

Following gene: 156976899

Centisome position: 40.27

GC content: 47.11

Gene sequence:

>1524_bases
ATGAAAACATTCAACCTTAAACCCACACTTTTACCTTTAACTTTGCTGCTCAGTTCGCCAGTAATGGCGGCTCAAAATGG
AACCATGATGCAGTATTTCCATTGGTATGTGCTAAATGACGGTGCACTCTGGACACAAGTTGAAAACAACGCGCCAGCAC
TATCGGAAAACGGCTTTACCGCGTTATGGCTACCGCCTGCCTATAAAGGTGCTGGTGGCAGCAACGACGTCGGTTATGGC
GTTTACGATATGTATGACTTGGGGGAGTTTGATCAAAAAGGATCGGTGCGAACCAAGTACGGTACAAAAGACCAATACCT
CGATGCCATCAAAGCGGCACACGCAAACAACATTCAAATTTATGGTGATGTGGTGTTTAACCACCGTGGCGGTGCGGATG
GTAAATCGTGGGTCGACACCGAGCGAGTCGATTGGAACAATCGGAACATTGAACTCGGCGACAAATGGATTGAAGCTTGG
GTCGAATTTAGCTTTCCGGGGCGTAACGACAAATACTCAGATTTCCATTGGACGTGGTATCACTTTGACGGCGTTGACTG
GGATGACGCAGGTAAAGAAAAAGCGATCTTCAAATTCAAAGGCGATGGCAAAGCATGGGATTGGGAAGTCAGTTCTGAAA
AAGGCAACTATGACTACCTCATGTACGCAGATTTAGACATGGACCACCCAGAAGTGAAGCAAGAACTGAAAGACTGGGGC
GATTGGTACTTAAATATGACGGGTGTGGATGGCTTCCGAATGGACGCAGTCAAACACATCAAATATCAATACCTGCAAGA
GTGGATCGATTACTTGCGACAGAAAACGAGCAAAGAGTTGTTTACTGTCGGTGAGTACTGGAACTACGACGTTAACAATC
TGCACAACTTTATCACCAAGACGTCTGGCAGTATGTCGTTGTTTGATGCACCGTTACACATGAACTTCTACAACGCTTCA
CGCTCAGGTGGTAACTTCGACATGCGTCGTATCATGGATGGTACCTTGATGAAGGACAACCCAGTGAAAGCAGTGACTCT
GGTTGAGAACCACGATACGCAGCCTCTTCAAGCGCTAGAATCTCCAGTCGATTGGTGGTTTAAGTCTCTCGCGTACGCGT
TTATCTTGCTACGTGAAGAGGGCTATCCGTCAGTCTTTTATGCTGATTACTACGGCGCGCAATACAGTGATAAAGGGCAC
GACATCAACATGGTGAAAGTGCCGTATATTGAGAAATTGGTGAAAGCCCGTAAAGATTATGCTTATGGCAAGCAGCACTC
TTACCTTGATCATTGGGATGTGATTGGTTGGACGCGAGAAGGGGATGCAGAACACCCGAACTCAATGGCGGTTATCATGA
GTGATGGCCCTGGCGGTACAAAATGGATGTACACAGGTTCACCAAGTACGCGTTACGTCGATAAGTTAGGTATTCGCACG
GAAGAAGTTTGGACTGACGCCAACGGTTGGGCAGAGTTCCCAGTGAACGGTGGTTCGGTTTCTGTTTGGGTCGGCGTTAA
ATAA

Upstream 100 bases:

>100_bases
TCCGAACTTTTTTGTACAACAGACGGTTAGTTGGTTCTCTTCTAATCGTCATGTTGGCTCTCTAAGTTCGGACTTTGTCC
TAAAACGTGAGAGAAAAATA

Downstream 100 bases:

>100_bases
ACAGGCTCACAGAATTGTAGGTGTGACCAATTTCGAAAATAGTGCAAATTTAATCAATGTCTTGTTTGATATTCATAAAA
CATATGCGTAACTTTATAGC

Product: cytoplasmic alpha-amylase

Products: NA

Alternate protein names: 1,4-alpha-D-glucan glucanohydrolase [H]

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MKTFNLKPTLLPLTLLLSSPVMAAQNGTMMQYFHWYVLNDGALWTQVENNAPALSENGFTALWLPPAYKGAGGSNDVGYG
VYDMYDLGEFDQKGSVRTKYGTKDQYLDAIKAAHANNIQIYGDVVFNHRGGADGKSWVDTERVDWNNRNIELGDKWIEAW
VEFSFPGRNDKYSDFHWTWYHFDGVDWDDAGKEKAIFKFKGDGKAWDWEVSSEKGNYDYLMYADLDMDHPEVKQELKDWG
DWYLNMTGVDGFRMDAVKHIKYQYLQEWIDYLRQKTSKELFTVGEYWNYDVNNLHNFITKTSGSMSLFDAPLHMNFYNAS
RSGGNFDMRRIMDGTLMKDNPVKAVTLVENHDTQPLQALESPVDWWFKSLAYAFILLREEGYPSVFYADYYGAQYSDKGH
DINMVKVPYIEKLVKARKDYAYGKQHSYLDHWDVIGWTREGDAEHPNSMAVIMSDGPGGTKWMYTGSPSTRYVDKLGIRT
EEVWTDANGWAEFPVNGGSVSVWVGVK

Sequences:

>Translated_507_residues
MKTFNLKPTLLPLTLLLSSPVMAAQNGTMMQYFHWYVLNDGALWTQVENNAPALSENGFTALWLPPAYKGAGGSNDVGYG
VYDMYDLGEFDQKGSVRTKYGTKDQYLDAIKAAHANNIQIYGDVVFNHRGGADGKSWVDTERVDWNNRNIELGDKWIEAW
VEFSFPGRNDKYSDFHWTWYHFDGVDWDDAGKEKAIFKFKGDGKAWDWEVSSEKGNYDYLMYADLDMDHPEVKQELKDWG
DWYLNMTGVDGFRMDAVKHIKYQYLQEWIDYLRQKTSKELFTVGEYWNYDVNNLHNFITKTSGSMSLFDAPLHMNFYNAS
RSGGNFDMRRIMDGTLMKDNPVKAVTLVENHDTQPLQALESPVDWWFKSLAYAFILLREEGYPSVFYADYYGAQYSDKGH
DINMVKVPYIEKLVKARKDYAYGKQHSYLDHWDVIGWTREGDAEHPNSMAVIMSDGPGGTKWMYTGSPSTRYVDKLGIRT
EEVWTDANGWAEFPVNGGSVSVWVGVK
>Mature_507_residues
MKTFNLKPTLLPLTLLLSSPVMAAQNGTMMQYFHWYVLNDGALWTQVENNAPALSENGFTALWLPPAYKGAGGSNDVGYG
VYDMYDLGEFDQKGSVRTKYGTKDQYLDAIKAAHANNIQIYGDVVFNHRGGADGKSWVDTERVDWNNRNIELGDKWIEAW
VEFSFPGRNDKYSDFHWTWYHFDGVDWDDAGKEKAIFKFKGDGKAWDWEVSSEKGNYDYLMYADLDMDHPEVKQELKDWG
DWYLNMTGVDGFRMDAVKHIKYQYLQEWIDYLRQKTSKELFTVGEYWNYDVNNLHNFITKTSGSMSLFDAPLHMNFYNAS
RSGGNFDMRRIMDGTLMKDNPVKAVTLVENHDTQPLQALESPVDWWFKSLAYAFILLREEGYPSVFYADYYGAQYSDKGH
DINMVKVPYIEKLVKARKDYAYGKQHSYLDHWDVIGWTREGDAEHPNSMAVIMSDGPGGTKWMYTGSPSTRYVDKLGIRT
EEVWTDANGWAEFPVNGGSVSVWVGVK

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1788236, Length=491, Percent_Identity=46.0285132382892, Blast_Score=429, Evalue=1e-121,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013776
- InterPro:   IPR015237
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF09154 DUF1939 [H]

EC number: =3.2.1.1 [H]

Molecular weight: Translated: 58378; Mature: 58378

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTFNLKPTLLPLTLLLSSPVMAAQNGTMMQYFHWYVLNDGALWTQVENNAPALSENGFT
CCCCCCCCHHHHHHHHHCCCCEECCCCCEEEEEEEEEEECCEEEEEECCCCCCCCCCCEE
ALWLPPAYKGAGGSNDVGYGVYDMYDLGEFDQKGSVRTKYGTKDQYLDAIKAAHANNIQI
EEECCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCEEECCCCHHHHHHHHHHCCCCCEEE
YGDVVFNHRGGADGKSWVDTERVDWNNRNIELGDKWIEAWVEFSFPGRNDKYSDFHWTWY
EEEEEEECCCCCCCCCCCCCCCCCCCCCCEECCHHHHHHHEEEECCCCCCCCCCEEEEEE
HFDGVDWDDAGKEKAIFKFKGDGKAWDWEVSSEKGNYDYLMYADLDMDHPEVKQELKDWG
EECCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCEEEEEEEECCCCCHHHHHHHHCCC
DWYLNMTGVDGFRMDAVKHIKYQYLQEWIDYLRQKTSKELFTVGEYWNYDVNNLHNFITK
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHC
TSGSMSLFDAPLHMNFYNASRSGGNFDMRRIMDGTLMKDNPVKAVTLVENHDTQPLQALE
CCCCCEEEECCEEEEEECCCCCCCCCHHHHHHCCCCCCCCCCEEEEEEECCCCCHHHHHH
SPVDWWFKSLAYAFILLREEGYPSVFYADYYGAQYSDKGHDINMVKVPYIEKLVKARKDY
CHHHHHHHHHHHEEEEEECCCCCEEEEEECCCCCCCCCCCCEEEEECHHHHHHHHHHHHH
AYGKQHSYLDHWDVIGWTREGDAEHPNSMAVIMSDGPGGTKWMYTGSPSTRYVDKLGIRT
CCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCCEEEEECCCCCHHHHHHCCCH
EEVWTDANGWAEFPVNGGSVSVWVGVK
HHEEECCCCEEECCCCCCCEEEEEECC
>Mature Secondary Structure
MKTFNLKPTLLPLTLLLSSPVMAAQNGTMMQYFHWYVLNDGALWTQVENNAPALSENGFT
CCCCCCCCHHHHHHHHHCCCCEECCCCCEEEEEEEEEEECCEEEEEECCCCCCCCCCCEE
ALWLPPAYKGAGGSNDVGYGVYDMYDLGEFDQKGSVRTKYGTKDQYLDAIKAAHANNIQI
EEECCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCEEECCCCHHHHHHHHHHCCCCCEEE
YGDVVFNHRGGADGKSWVDTERVDWNNRNIELGDKWIEAWVEFSFPGRNDKYSDFHWTWY
EEEEEEECCCCCCCCCCCCCCCCCCCCCCEECCHHHHHHHEEEECCCCCCCCCCEEEEEE
HFDGVDWDDAGKEKAIFKFKGDGKAWDWEVSSEKGNYDYLMYADLDMDHPEVKQELKDWG
EECCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCEEEEEEEECCCCCHHHHHHHHCCC
DWYLNMTGVDGFRMDAVKHIKYQYLQEWIDYLRQKTSKELFTVGEYWNYDVNNLHNFITK
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHC
TSGSMSLFDAPLHMNFYNASRSGGNFDMRRIMDGTLMKDNPVKAVTLVENHDTQPLQALE
CCCCCEEEECCEEEEEECCCCCCCCCHHHHHHCCCCCCCCCCEEEEEEECCCCCHHHHHH
SPVDWWFKSLAYAFILLREEGYPSVFYADYYGAQYSDKGHDINMVKVPYIEKLVKARKDY
CHHHHHHHHHHHEEEEEECCCCCEEEEEECCCCCCCCCCCCEEEEECHHHHHHHHHHHHH
AYGKQHSYLDHWDVIGWTREGDAEHPNSMAVIMSDGPGGTKWMYTGSPSTRYVDKLGIRT
CCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCCEEEEECCCCCHHHHHHCCCH
EEVWTDANGWAEFPVNGGSVSVWVGVK
HHEEECCCCEEECCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3924897; 3876333; 3009417; 2999073; 11226887 [H]