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The map label for this gene is hsdS [H]

Identifier: 156976838

GI number: 156976838

Start: 817716

End: 819014

Strand: Direct

Name: hsdS [H]

Synonym: VIBHAR_05614

Alternate gene names: 156976838

Gene position: 817716-819014 (Clockwise)

Preceding gene: 156976837

Following gene: 156976839

Centisome position: 37.1

GC content: 42.57

Gene sequence:

>1299_bases
ATGAATGAAAAGAAATGTTATCCATTACAGCGTTTAGGTGAGCTAGCTTCGGGAAATCGGGGAGTTTCTTACAAGCCCGA
GAATCTAAAAGCAGCGATTGATGATAAGTCAGTGGTTTTTCTTCGTTCCAACAATATTCAAAGTGGAACATTGAATTTTG
AAAATGTGCAGATTGTGCCTGATTCACTTGTTTCTGATAGCCAGATATTGAAGAAAGGCGATATTGCTGTTTGTATGTCA
AATGGTAGTCGTCAACTGGTTGGAAAAAGTGGAATGCTTCAACATGAGGTCGAGTATCCTCTAACGGTTGGAGCGTTTTG
TTCTGTATTTCGTTGCCAAAATGAAGATGATTCAGAATACGTTCGTTACCTTTTTCAATCACAAGCCTATCAACATGGAA
TTGATGTTACGCTTGCTGGTAGTGCAATAAATAATTTAAAGAACTCTGATGTTGAGGCTATTGAAGTACCGACGGCACCC
AAAGCATTGCGTAAGAAAATTGCTGAAATATTGTCAACCATCGACAACCAAATCGACGCCACTCAAGCCCTAATCGACAA
ATACACTGCTATCAAACAAGGCATGATGGCGGATTTGTTTAGCCGAGGTATCGACCCGGAAACTAAAGCACTTCGCCCAA
CGCTTGAAGAAGCGCCAGAGCTTTATCATAAGACCCCGCTGGGGATGTTGCCTAAGGGGTGGGATGTAAAAACACTTGGA
GATATATCAGAAAAGATCACAAGTGGCTCTAGAGACTGGGCGAAGTTTTACTCTCCAGAGGGCGATCTTTTTGTTCGGAT
CAGTAATCTTACTCGCGAGCATGTGAACTTTCGTTGGGATTCCGTTAAACATGTCAATATCGGCGGTGGCTCTGAGGGGG
AAAGAACACAGCTACAGCCGGGTGACATTCTCGTTTCGATTACTGCAGATTTAGGTATTGTTGGTGTTGTCCCTGAGAAT
ATGGGGAGAGCCTATATTAACCAACATACGGCACTAATTCGCTTATCCACTTATGGTGAAAATGCGAGATTTATTGGTAA
TTACTTGTCATCTCGATGTGGGCAGGAGCAGTTTGAGAAAAACAATGATTCAGGTGCAAAAGCTGGTATCAATTTACCTA
CAATCGCCTCGCTTCGCTGTCCAATCCCCGAGGAAAAAGAGCAATTATTAATAGCATCAAAAATCGATGCTTTAGACGAG
GTTATTGCTGACCTCAAAAGGGAAAAGTCGAAAAGCCTTTCACTCAAGCAAGGCTTAATGCAGGACCTCTTAACAGGCAA
AGTCTCCGTACCCGCATAA

Upstream 100 bases:

>100_bases
GAGAACCTACACCGCAAATACCAAGTTACTGCCCAAAGCATCATTGCCAAGCGAGAGCAGGAAGCGAACAAGTTAGCTGG
CTTTATGAAGGAGCTGGGCT

Downstream 100 bases:

>100_bases
CAAAAAAAGAAAAATGGCAGCAGGCAAATGCCTCGCTGCCAGCAATAAGGATATCAAACAATGTCAGAGTACCAATTGGT
AGAAAAGCCGCTGCTAAACC

Product: type I restriction-modification system S subunit

Products: NA

Alternate protein names: S.EcoKI; Type I restriction enzyme EcoKI specificity protein; S protein [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MNEKKCYPLQRLGELASGNRGVSYKPENLKAAIDDKSVVFLRSNNIQSGTLNFENVQIVPDSLVSDSQILKKGDIAVCMS
NGSRQLVGKSGMLQHEVEYPLTVGAFCSVFRCQNEDDSEYVRYLFQSQAYQHGIDVTLAGSAINNLKNSDVEAIEVPTAP
KALRKKIAEILSTIDNQIDATQALIDKYTAIKQGMMADLFSRGIDPETKALRPTLEEAPELYHKTPLGMLPKGWDVKTLG
DISEKITSGSRDWAKFYSPEGDLFVRISNLTREHVNFRWDSVKHVNIGGGSEGERTQLQPGDILVSITADLGIVGVVPEN
MGRAYINQHTALIRLSTYGENARFIGNYLSSRCGQEQFEKNNDSGAKAGINLPTIASLRCPIPEEKEQLLIASKIDALDE
VIADLKREKSKSLSLKQGLMQDLLTGKVSVPA

Sequences:

>Translated_432_residues
MNEKKCYPLQRLGELASGNRGVSYKPENLKAAIDDKSVVFLRSNNIQSGTLNFENVQIVPDSLVSDSQILKKGDIAVCMS
NGSRQLVGKSGMLQHEVEYPLTVGAFCSVFRCQNEDDSEYVRYLFQSQAYQHGIDVTLAGSAINNLKNSDVEAIEVPTAP
KALRKKIAEILSTIDNQIDATQALIDKYTAIKQGMMADLFSRGIDPETKALRPTLEEAPELYHKTPLGMLPKGWDVKTLG
DISEKITSGSRDWAKFYSPEGDLFVRISNLTREHVNFRWDSVKHVNIGGGSEGERTQLQPGDILVSITADLGIVGVVPEN
MGRAYINQHTALIRLSTYGENARFIGNYLSSRCGQEQFEKNNDSGAKAGINLPTIASLRCPIPEEKEQLLIASKIDALDE
VIADLKREKSKSLSLKQGLMQDLLTGKVSVPA
>Mature_432_residues
MNEKKCYPLQRLGELASGNRGVSYKPENLKAAIDDKSVVFLRSNNIQSGTLNFENVQIVPDSLVSDSQILKKGDIAVCMS
NGSRQLVGKSGMLQHEVEYPLTVGAFCSVFRCQNEDDSEYVRYLFQSQAYQHGIDVTLAGSAINNLKNSDVEAIEVPTAP
KALRKKIAEILSTIDNQIDATQALIDKYTAIKQGMMADLFSRGIDPETKALRPTLEEAPELYHKTPLGMLPKGWDVKTLG
DISEKITSGSRDWAKFYSPEGDLFVRISNLTREHVNFRWDSVKHVNIGGGSEGERTQLQPGDILVSITADLGIVGVVPEN
MGRAYINQHTALIRLSTYGENARFIGNYLSSRCGQEQFEKNNDSGAKAGINLPTIASLRCPIPEEKEQLLIASKIDALDE
VIADLKREKSKSLSLKQGLMQDLLTGKVSVPA

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

Organism=Escherichia coli, GI1790807, Length=429, Percent_Identity=26.1072261072261, Blast_Score=112, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 47536; Mature: 47536

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEKKCYPLQRLGELASGNRGVSYKPENLKAAIDDKSVVFLRSNNIQSGTLNFENVQIVP
CCCCCCCCHHHHHHHHCCCCCCCCCCCCCEEEECCCEEEEEECCCCCCCCCCCCEEEECC
DSLVSDSQILKKGDIAVCMSNGSRQLVGKSGMLQHEVEYPLTVGAFCSVFRCQNEDDSEY
HHHCCCHHHHHCCCEEEEECCCCCEECCCCCCCEECCCCCEEHHHHHHHHHCCCCCHHHH
VRYLFQSQAYQHGIDVTLAGSAINNLKNSDVEAIEVPTAPKALRKKIAEILSTIDNQIDA
HHHHHHHHHHHCCCCEEECCHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHH
TQALIDKYTAIKQGMMADLFSRGIDPETKALRPTLEEAPELYHKTPLGMLPKGWDVKTLG
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCHHH
DISEKITSGSRDWAKFYSPEGDLFVRISNLTREHVNFRWDSVKHVNIGGGSEGERTQLQP
HHHHHHHCCCCHHHHHCCCCCCEEEEEEHHHHHHCCEEECCEEEEECCCCCCCCCCCCCC
GDILVSITADLGIVGVVPENMGRAYINQHTALIRLSTYGENARFIGNYLSSRCGQEQFEK
CCEEEEEECCCCEEEECCCCCCHHHHCCCEEEEEEECCCCCHHHHHHHHHHHCCHHHHHC
NNDSGAKAGINLPTIASLRCPIPEEKEQLLIASKIDALDEVIADLKREKSKSLSLKQGLM
CCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QDLLTGKVSVPA
HHHHCCCCCCCC
>Mature Secondary Structure
MNEKKCYPLQRLGELASGNRGVSYKPENLKAAIDDKSVVFLRSNNIQSGTLNFENVQIVP
CCCCCCCCHHHHHHHHCCCCCCCCCCCCCEEEECCCEEEEEECCCCCCCCCCCCEEEECC
DSLVSDSQILKKGDIAVCMSNGSRQLVGKSGMLQHEVEYPLTVGAFCSVFRCQNEDDSEY
HHHCCCHHHHHCCCEEEEECCCCCEECCCCCCCEECCCCCEEHHHHHHHHHCCCCCHHHH
VRYLFQSQAYQHGIDVTLAGSAINNLKNSDVEAIEVPTAPKALRKKIAEILSTIDNQIDA
HHHHHHHHHHHCCCCEEECCHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHH
TQALIDKYTAIKQGMMADLFSRGIDPETKALRPTLEEAPELYHKTPLGMLPKGWDVKTLG
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCHHH
DISEKITSGSRDWAKFYSPEGDLFVRISNLTREHVNFRWDSVKHVNIGGGSEGERTQLQP
HHHHHHHCCCCHHHHHCCCCCCEEEEEEHHHHHHCCEEECCEEEEECCCCCCCCCCCCCC
GDILVSITADLGIVGVVPENMGRAYINQHTALIRLSTYGENARFIGNYLSSRCGQEQFEK
CCEEEEEECCCCEEEECCCCCCHHHHCCCEEEEEEECCCCCHHHHHHHHHHHCCHHHHHC
NNDSGAKAGINLPTIASLRCPIPEEKEQLLIASKIDALDEVIADLKREKSKSLSLKQGLM
CCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QDLLTGKVSVPA
HHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6304321; 7610040; 9278503; 7739896 [H]