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The map label for this gene is fadH [C]

Identifier: 156976721

GI number: 156976721

Start: 707462

End: 708592

Strand: Direct

Name: fadH [C]

Synonym: VIBHAR_05496

Alternate gene names: 156976721

Gene position: 707462-708592 (Clockwise)

Preceding gene: 156976720

Following gene: 156976722

Centisome position: 32.1

GC content: 48.54

Gene sequence:

>1131_bases
ATGTATCTAAAAAGACAGATTTTAAGAGGACTGGACTTGAGCATTTTGTTTTCAGAAGCCCGCATTGGCAACATGACACT
AAAGAACCGCTTTGTACGAAGTGCAACGTGGGAAAATATGGCAACGGAAGACGGTCACATGACCGAGAAGCTGTACGACA
TTTATGAAGAACTCGCCAAGGGTGAAGTGGGGCTGATCGTTACTGGCTACGCGAACATCGTAGAAGAAGAAAAGCCGAAC
GCAGGCATGATGGGGATCTACAATGACTCTTTCATCGATGGATACAAAAAGCTGACTGACCTTGTCCATGAGCATGATGC
TAAGATTGTCATGCAGATCGCTTACGGCGGCACAAAAACGACCTTCAACGTTGGCGAGCGTGTGATTTTTGCTCCAAGTG
AAGTGCCAGAGCGTGGCACCAAAACGGAAGGCAAAGCGATGACTCAAGATGAGATTGACTACATCGTTAAAGCATTTGCG
CTAGCGAGCAAGCGTGCACAAGCTTCTGGCTTTGATGGTGTAGAAATCCACGCGGCGCACACGTACCTGATCAACCAGTT
CTTAAGCCCATATTACAACCGTCGCGAAGACCAGTACGGCGGCAGCCTAGAGAACCGCATGCGTTTTCTTATGGAGATCT
ATGACGAAACACGCAAGCTAGTTGGTGATGATTTCCCGATTCTGGTGAAGCTGACAGCAACGGAATTCTTCGATGGTGGT
TTGACGTTCGATGAAACGCGCATTGTTTGTAAGAAGCTAGAAGAAGTGGGCGTGGATGCGATCATTGTATCGGGTAACAT
TCACGGCAACGCAAGCACCATGGTAGGTGAATCGTTCGACGGTTACGCGCTACAAGAAGAAGGTTACTTCCACGAATACG
GCCAAGTGATCAGCCAAGATGTGAGCGTACCTGTGATTACCGTGGGTGGCTTAAACGACTTCTGTGCGATTGAAGACTTG
GCTGAAAATACCAAGATTCAATTCTTCGCGCTTTCTCGTCCATTATTGGCAGAGCCACAATTGATCAAGCGCTGGAAAGA
CGGTAACCGCGCCGAAGTGGACTGCGAACGCTGCTCTAAGTGTCGCACCAAGCGTGGCAACTTCTGCGTGGTTTACAAGC
GCAGAAAGTAG

Upstream 100 bases:

>100_bases
CGGTAAGGTTCGTGTCGCTCATTGCACTGAACAGAAATTCAGAGAAGGAACAGCCATGCTTTGTGTATCGCTGTTCCTTT
TGCGTTCCTGCTCGGTAGTG

Downstream 100 bases:

>100_bases
TGCGTTCTAGCTCATGAAATTTTAAAAAGCCCCTGATGCTTAACGTGTCAGGGGCTTTTTTAGTTGTGAGTTATACCCAA
GTAACCTCGAGATGCTGGTT

Product: NADH-dependent flavin oxidoreductase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MYLKRQILRGLDLSILFSEARIGNMTLKNRFVRSATWENMATEDGHMTEKLYDIYEELAKGEVGLIVTGYANIVEEEKPN
AGMMGIYNDSFIDGYKKLTDLVHEHDAKIVMQIAYGGTKTTFNVGERVIFAPSEVPERGTKTEGKAMTQDEIDYIVKAFA
LASKRAQASGFDGVEIHAAHTYLINQFLSPYYNRREDQYGGSLENRMRFLMEIYDETRKLVGDDFPILVKLTATEFFDGG
LTFDETRIVCKKLEEVGVDAIIVSGNIHGNASTMVGESFDGYALQEEGYFHEYGQVISQDVSVPVITVGGLNDFCAIEDL
AENTKIQFFALSRPLLAEPQLIKRWKDGNRAEVDCERCSKCRTKRGNFCVVYKRRK

Sequences:

>Translated_376_residues
MYLKRQILRGLDLSILFSEARIGNMTLKNRFVRSATWENMATEDGHMTEKLYDIYEELAKGEVGLIVTGYANIVEEEKPN
AGMMGIYNDSFIDGYKKLTDLVHEHDAKIVMQIAYGGTKTTFNVGERVIFAPSEVPERGTKTEGKAMTQDEIDYIVKAFA
LASKRAQASGFDGVEIHAAHTYLINQFLSPYYNRREDQYGGSLENRMRFLMEIYDETRKLVGDDFPILVKLTATEFFDGG
LTFDETRIVCKKLEEVGVDAIIVSGNIHGNASTMVGESFDGYALQEEGYFHEYGQVISQDVSVPVITVGGLNDFCAIEDL
AENTKIQFFALSRPLLAEPQLIKRWKDGNRAEVDCERCSKCRTKRGNFCVVYKRRK
>Mature_376_residues
MYLKRQILRGLDLSILFSEARIGNMTLKNRFVRSATWENMATEDGHMTEKLYDIYEELAKGEVGLIVTGYANIVEEEKPN
AGMMGIYNDSFIDGYKKLTDLVHEHDAKIVMQIAYGGTKTTFNVGERVIFAPSEVPERGTKTEGKAMTQDEIDYIVKAFA
LASKRAQASGFDGVEIHAAHTYLINQFLSPYYNRREDQYGGSLENRMRFLMEIYDETRKLVGDDFPILVKLTATEFFDGG
LTFDETRIVCKKLEEVGVDAIIVSGNIHGNASTMVGESFDGYALQEEGYFHEYGQVISQDVSVPVITVGGLNDFCAIEDL
AENTKIQFFALSRPLLAEPQLIKRWKDGNRAEVDCERCSKCRTKRGNFCVVYKRRK

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=351, Percent_Identity=28.4900284900285, Blast_Score=141, Evalue=9e-35,
Organism=Escherichia coli, GI1787939, Length=363, Percent_Identity=27.8236914600551, Blast_Score=112, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI17559802, Length=345, Percent_Identity=30.1449275362319, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17540738, Length=348, Percent_Identity=31.0344827586207, Blast_Score=142, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17565138, Length=337, Percent_Identity=29.3768545994065, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI72001454, Length=359, Percent_Identity=28.9693593314763, Blast_Score=134, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI17559804, Length=341, Percent_Identity=29.0322580645161, Blast_Score=127, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI17564188, Length=337, Percent_Identity=29.3768545994065, Blast_Score=127, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI17566914, Length=302, Percent_Identity=29.1390728476821, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17566916, Length=354, Percent_Identity=25.9887005649718, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6321973, Length=250, Percent_Identity=28.4, Blast_Score=97, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6325086, Length=223, Percent_Identity=30.0448430493274, Blast_Score=94, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 42524; Mature: 42524

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLKRQILRGLDLSILFSEARIGNMTLKNRFVRSATWENMATEDGHMTEKLYDIYEELAK
CCHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHC
GEVGLIVTGYANIVEEEKPNAGMMGIYNDSFIDGYKKLTDLVHEHDAKIVMQIAYGGTKT
CCEEEEEECCHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCE
TFNVGERVIFAPSEVPERGTKTEGKAMTQDEIDYIVKAFALASKRAQASGFDGVEIHAAH
EEECCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHH
TYLINQFLSPYYNRREDQYGGSLENRMRFLMEIYDETRKLVGDDFPILVKLTATEFFDGG
HHHHHHHHHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHCCCC
LTFDETRIVCKKLEEVGVDAIIVSGNIHGNASTMVGESFDGYALQEEGYFHEYGQVISQD
CCCHHHHHHHHHHHHCCCCEEEEECCCCCCCHHCCCCCCCCEEECCCCCHHHHHHHHHCC
VSVPVITVGGLNDFCAIEDLAENTKIQFFALSRPLLAEPQLIKRWKDGNRAEVDCERCSK
CCCEEEEECCCCCHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHCCCCCCCEECHHHHHH
CRTKRGNFCVVYKRRK
HHCCCCCEEEEEECCC
>Mature Secondary Structure
MYLKRQILRGLDLSILFSEARIGNMTLKNRFVRSATWENMATEDGHMTEKLYDIYEELAK
CCHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHC
GEVGLIVTGYANIVEEEKPNAGMMGIYNDSFIDGYKKLTDLVHEHDAKIVMQIAYGGTKT
CCEEEEEECCHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCE
TFNVGERVIFAPSEVPERGTKTEGKAMTQDEIDYIVKAFALASKRAQASGFDGVEIHAAH
EEECCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHH
TYLINQFLSPYYNRREDQYGGSLENRMRFLMEIYDETRKLVGDDFPILVKLTATEFFDGG
HHHHHHHHHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHCCCC
LTFDETRIVCKKLEEVGVDAIIVSGNIHGNASTMVGESFDGYALQEEGYFHEYGQVISQD
CCCHHHHHHHHHHHHCCCCEEEEECCCCCCCHHCCCCCCCCEEECCCCCHHHHHHHHHCC
VSVPVITVGGLNDFCAIEDLAENTKIQFFALSRPLLAEPQLIKRWKDGNRAEVDCERCSK
CCCEEEEECCCCCHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHCCCCCCCEECHHHHHH
CRTKRGNFCVVYKRRK
HHCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]