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The map label for this gene is paaF [H]

Identifier: 156976710

GI number: 156976710

Start: 698166

End: 698954

Strand: Direct

Name: paaF [H]

Synonym: VIBHAR_05485

Alternate gene names: 156976710

Gene position: 698166-698954 (Clockwise)

Preceding gene: 156976709

Following gene: 156976711

Centisome position: 31.68

GC content: 49.56

Gene sequence:

>789_bases
ATGTCATTACCAGAACAACAAGCGATTCAACACACCATCAGCAATCACGTTGCGGTGGTGACCATGAACAACCCGCCTGC
CAATACTTGGACGTCAGGAAGTTTAACGGCATTAAAGGAATTGGTGCTTGCGTTAAACGACAACAAAGACGTTTACGCTC
TAGTGCTCACAGGGAATGGTGAGAAGTTTTTCTCGGCTGGTGCAGACCTCAAGTTGTTTGCCGATGGCGATAAAGCGGTT
GCGTTAGAGATGGCGCGGATCTTTGGTGAAGCCTTTGAAACGCTGTCTGCATTTCGAGGGGTTTCCATCGCGGCCATCAA
CGGTTATGCCATGGGAGGCGGATTGGAAGTCGCATTGGCGTGTGATATTCGCGTGGTGGAAGAGCAAGCCGTGCTGGCAC
TGCCAGAGGCGAAAGTGGGCTTACTGCCTTGTGCTGGTGGTACGCAAAACCTAACCGCATTGGTCGGTGAAGGTTGGGCG
AAACGCATCATATTGTGCGGTGAACAAGTCGGTGCAGCACAAGCGCGAGAACTTGGGTTAGTAGAAGAAGTGGTGGAAAA
AGGTGTAGCGCTGAATAAAGCCATTGAGCTTGCAGAATCGGTTGCCAATCAATCGCCGTCTTCGGTAACTGCATGTAAAG
CGCTTATCCAAAACATGCGTTCAGCGCCACTCAAACATGGTCTGATTAAAGAGCGAGAACTGTTTCTAAACTTATTCGAT
ACCGAAGATCAAACCGAGGGGGTTCGTGCCTTCTTGGAAAAACGTAAACCCAACTGGAAGAATCAATAG

Upstream 100 bases:

>100_bases
GATTGCTCGCAGAGGAGTCGGCATTGTTATAGACGCAACGCCAAGCTCTGCTTTTATTCATAAAACTCAGGTCATTTTCA
TCAAGAAAGGATTCAGAGAT

Downstream 100 bases:

>100_bases
GAGGTCGCATGACAGGGAAAGTAACCATTAGCGAAATAGATTGCTTAGACGGCGTGCACCGAATTGGTATCGCGACGTTA
GACAACACGGCTTCTTTGAA

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MSLPEQQAIQHTISNHVAVVTMNNPPANTWTSGSLTALKELVLALNDNKDVYALVLTGNGEKFFSAGADLKLFADGDKAV
ALEMARIFGEAFETLSAFRGVSIAAINGYAMGGGLEVALACDIRVVEEQAVLALPEAKVGLLPCAGGTQNLTALVGEGWA
KRIILCGEQVGAAQARELGLVEEVVEKGVALNKAIELAESVANQSPSSVTACKALIQNMRSAPLKHGLIKERELFLNLFD
TEDQTEGVRAFLEKRKPNWKNQ

Sequences:

>Translated_262_residues
MSLPEQQAIQHTISNHVAVVTMNNPPANTWTSGSLTALKELVLALNDNKDVYALVLTGNGEKFFSAGADLKLFADGDKAV
ALEMARIFGEAFETLSAFRGVSIAAINGYAMGGGLEVALACDIRVVEEQAVLALPEAKVGLLPCAGGTQNLTALVGEGWA
KRIILCGEQVGAAQARELGLVEEVVEKGVALNKAIELAESVANQSPSSVTACKALIQNMRSAPLKHGLIKERELFLNLFD
TEDQTEGVRAFLEKRKPNWKNQ
>Mature_261_residues
SLPEQQAIQHTISNHVAVVTMNNPPANTWTSGSLTALKELVLALNDNKDVYALVLTGNGEKFFSAGADLKLFADGDKAVA
LEMARIFGEAFETLSAFRGVSIAAINGYAMGGGLEVALACDIRVVEEQAVLALPEAKVGLLPCAGGTQNLTALVGEGWAK
RIILCGEQVGAAQARELGLVEEVVEKGVALNKAIELAESVANQSPSSVTACKALIQNMRSAPLKHGLIKERELFLNLFDT
EDQTEGVRAFLEKRKPNWKNQ

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=254, Percent_Identity=35.8267716535433, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI68989263, Length=280, Percent_Identity=30.7142857142857, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI4502327, Length=239, Percent_Identity=32.2175732217573, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI20127408, Length=235, Percent_Identity=31.9148936170213, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI31542718, Length=252, Percent_Identity=26.984126984127, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI213417737, Length=226, Percent_Identity=24.7787610619469, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI157694516, Length=226, Percent_Identity=24.7787610619469, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI150378541, Length=245, Percent_Identity=29.3877551020408, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI261878539, Length=183, Percent_Identity=32.7868852459016, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI70995211, Length=257, Percent_Identity=27.2373540856031, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI157694520, Length=202, Percent_Identity=24.7524752475248, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI62530384, Length=247, Percent_Identity=27.9352226720648, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI1787659, Length=249, Percent_Identity=39.3574297188755, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI221142681, Length=264, Percent_Identity=32.5757575757576, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1788682, Length=199, Percent_Identity=37.1859296482412, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1790281, Length=182, Percent_Identity=32.967032967033, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788597, Length=258, Percent_Identity=25.968992248062, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI87082183, Length=255, Percent_Identity=24.3137254901961, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1787660, Length=261, Percent_Identity=28.735632183908, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17554946, Length=254, Percent_Identity=37.007874015748, Blast_Score=151, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI25145438, Length=249, Percent_Identity=37.7510040160643, Blast_Score=150, Evalue=9e-37,
Organism=Caenorhabditis elegans, GI17540714, Length=201, Percent_Identity=32.8358208955224, Blast_Score=104, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17536985, Length=263, Percent_Identity=23.574144486692, Blast_Score=80, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17558304, Length=173, Percent_Identity=30.0578034682081, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI25144276, Length=175, Percent_Identity=32, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17534483, Length=231, Percent_Identity=25.1082251082251, Blast_Score=77, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17508953, Length=175, Percent_Identity=32, Blast_Score=77, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17508951, Length=175, Percent_Identity=32, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17560910, Length=220, Percent_Identity=30, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI25141363, Length=191, Percent_Identity=27.7486910994764, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI25144157, Length=179, Percent_Identity=30.7262569832402, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25144160, Length=179, Percent_Identity=30.7262569832402, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI20129971, Length=248, Percent_Identity=35.8870967741936, Blast_Score=143, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24653477, Length=248, Percent_Identity=35.8870967741936, Blast_Score=143, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24653139, Length=261, Percent_Identity=31.8007662835249, Blast_Score=118, Evalue=4e-27,
Organism=Drosophila melanogaster, GI21357171, Length=212, Percent_Identity=31.6037735849057, Blast_Score=91, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24650670, Length=190, Percent_Identity=33.1578947368421, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI19922422, Length=253, Percent_Identity=25.296442687747, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19920382, Length=190, Percent_Identity=30, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24583165, Length=254, Percent_Identity=27.1653543307087, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19921018, Length=195, Percent_Identity=28.7179487179487, Blast_Score=68, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27943; Mature: 27812

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLPEQQAIQHTISNHVAVVTMNNPPANTWTSGSLTALKELVLALNDNKDVYALVLTGNG
CCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCC
EKFFSAGADLKLFADGDKAVALEMARIFGEAFETLSAFRGVSIAAINGYAMGGGLEVALA
CEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEECCCEEEEEE
CDIRVVEEQAVLALPEAKVGLLPCAGGTQNLTALVGEGWAKRIILCGEQVGAAQARELGL
EEEEEECCCCEEECCCCCEEEEECCCCCCHHHHHHCCCCHHEEEEECCCCCHHHHHHHHH
VEEVVEKGVALNKAIELAESVANQSPSSVTACKALIQNMRSAPLKHGLIKERELFLNLFD
HHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHCHHHHHHHHEEEEC
TEDQTEGVRAFLEKRKPNWKNQ
CCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SLPEQQAIQHTISNHVAVVTMNNPPANTWTSGSLTALKELVLALNDNKDVYALVLTGNG
CCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCC
EKFFSAGADLKLFADGDKAVALEMARIFGEAFETLSAFRGVSIAAINGYAMGGGLEVALA
CEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEECCCEEEEEE
CDIRVVEEQAVLALPEAKVGLLPCAGGTQNLTALVGEGWAKRIILCGEQVGAAQARELGL
EEEEEECCCCEEECCCCCEEEEECCCCCCHHHHHHCCCCHHEEEEECCCCCHHHHHHHHH
VEEVVEKGVALNKAIELAESVANQSPSSVTACKALIQNMRSAPLKHGLIKERELFLNLFD
HHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHCHHHHHHHHEEEEC
TEDQTEGVRAFLEKRKPNWKNQ
CCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]