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The map label for this gene is paaF [H]

Identifier: 156976703

GI number: 156976703

Start: 689712

End: 690557

Strand: Reverse

Name: paaF [H]

Synonym: VIBHAR_05478

Alternate gene names: 156976703

Gene position: 690557-689712 (Counterclockwise)

Preceding gene: 156976704

Following gene: 156976702

Centisome position: 31.33

GC content: 48.58

Gene sequence:

>846_bases
ATGCAGGATCATCAACCGAGTATCATCCCCAATACAAAGCGACAAGATGAAGTACTTTGGAACGTCGATGAATTCGGCGT
CGCAACGCTAACGCTCAATCGTACAGAGAAGCACAATGCGTTTGATGCCTGTATGATTGACTTGCTGATCCATAGGCTTG
ACTACCTCGCTTTACGCACCGATGTTCGATGCTTAGTGCTTCGAGGAAACGGCAAGCACTTCTCCTCTGGCGCGGATTTA
AATTGGATGAAATCCATGGCAAACAGCACCAAAAAGCAAAACCTTGAAGACGCACAGAACCTCGCTCGTCTGATGCATAC
ACTGGACGCTTTCCCTCAGCCTACCATTGCGGTTGTGCAGGGTTCCGCATTTGGTGGCGCATTGGGCTTAATCTGTTGTT
GTGATATTGCCGTCGCGGCGGAGAATGCGAAGTTCTGCTTGAGTGAAGTCAAGCTAGGGCTGGTTCCGGCAACTATCTCT
CCTTATGTCATGCGTGCTATGGGAAACCGTCAAGCTCGTCGTTACATACTCAGTGCCGAAGTGATTAACGCCGCCAAAGC
AGAAAAGTTTGGCCTTGTGCATGAGGTTCATAAGCTGGATAAAATCGAAGCCGTGGTAGAAAGTTTGATTGATTCTTTGC
TGCTCAATAGCCCAGATGCAATGCGAAAAGCCAAAGCGTTATGCCATCAATGCCACCAAAATCCGATTGATGGTCAGCTT
ATCCAATACACGAGCCAACTGATCGCCGATATTCGCGTATCACCGCAAGGACAAGACGGCCTACAAGCCTTTCTGGAGAA
ACGAGAACCGGGTTGGATAAACAAGCCAGCTAAGGGCAAGAAATGA

Upstream 100 bases:

>100_bases
TCAAGCGTTAAGAGCATCGCTCCATGCGCCAATTCCTGACAGCAACTTTGGCATATTCAGAATGTAGCAATGTAGAAATC
TAGAGAAGAAAGGACGTCAC

Downstream 100 bases:

>100_bases
ATTTGCCAAACAAGGTAAATATTGTCGAAGTGGGTGCGAGAGACGGATTACAAAACGAATCGCCAGTGTCCACGCAAGCC
AAGATCCGCTTAATCGACCT

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MQDHQPSIIPNTKRQDEVLWNVDEFGVATLTLNRTEKHNAFDACMIDLLIHRLDYLALRTDVRCLVLRGNGKHFSSGADL
NWMKSMANSTKKQNLEDAQNLARLMHTLDAFPQPTIAVVQGSAFGGALGLICCCDIAVAAENAKFCLSEVKLGLVPATIS
PYVMRAMGNRQARRYILSAEVINAAKAEKFGLVHEVHKLDKIEAVVESLIDSLLLNSPDAMRKAKALCHQCHQNPIDGQL
IQYTSQLIADIRVSPQGQDGLQAFLEKREPGWINKPAKGKK

Sequences:

>Translated_281_residues
MQDHQPSIIPNTKRQDEVLWNVDEFGVATLTLNRTEKHNAFDACMIDLLIHRLDYLALRTDVRCLVLRGNGKHFSSGADL
NWMKSMANSTKKQNLEDAQNLARLMHTLDAFPQPTIAVVQGSAFGGALGLICCCDIAVAAENAKFCLSEVKLGLVPATIS
PYVMRAMGNRQARRYILSAEVINAAKAEKFGLVHEVHKLDKIEAVVESLIDSLLLNSPDAMRKAKALCHQCHQNPIDGQL
IQYTSQLIADIRVSPQGQDGLQAFLEKREPGWINKPAKGKK
>Mature_281_residues
MQDHQPSIIPNTKRQDEVLWNVDEFGVATLTLNRTEKHNAFDACMIDLLIHRLDYLALRTDVRCLVLRGNGKHFSSGADL
NWMKSMANSTKKQNLEDAQNLARLMHTLDAFPQPTIAVVQGSAFGGALGLICCCDIAVAAENAKFCLSEVKLGLVPATIS
PYVMRAMGNRQARRYILSAEVINAAKAEKFGLVHEVHKLDKIEAVVESLIDSLLLNSPDAMRKAKALCHQCHQNPIDGQL
IQYTSQLIADIRVSPQGQDGLQAFLEKREPGWINKPAKGKK

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=252, Percent_Identity=27.3809523809524, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI194097323, Length=256, Percent_Identity=29.296875, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI157694516, Length=212, Percent_Identity=27.8301886792453, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI213417737, Length=212, Percent_Identity=27.8301886792453, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI4502327, Length=255, Percent_Identity=30.1960784313725, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI45643119, Length=228, Percent_Identity=27.6315789473684, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI260275230, Length=228, Percent_Identity=27.6315789473684, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI260274832, Length=228, Percent_Identity=27.6315789473684, Blast_Score=75, Evalue=7e-14,
Organism=Escherichia coli, GI1787659, Length=247, Percent_Identity=33.6032388663968, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI87082183, Length=260, Percent_Identity=27.3076923076923, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788597, Length=250, Percent_Identity=28.8, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787660, Length=258, Percent_Identity=29.4573643410853, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI221142681, Length=263, Percent_Identity=27.7566539923954, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1790281, Length=180, Percent_Identity=28.8888888888889, Blast_Score=72, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17540714, Length=226, Percent_Identity=32.7433628318584, Blast_Score=97, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI25145438, Length=253, Percent_Identity=28.0632411067194, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17554946, Length=250, Percent_Identity=27.2, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17535521, Length=229, Percent_Identity=27.5109170305677, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17540306, Length=234, Percent_Identity=26.9230769230769, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17549921, Length=220, Percent_Identity=28.6363636363636, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17536985, Length=232, Percent_Identity=26.2931034482759, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17534483, Length=233, Percent_Identity=24.4635193133047, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24653139, Length=255, Percent_Identity=30.1960784313725, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI20129971, Length=255, Percent_Identity=27.0588235294118, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24653477, Length=255, Percent_Identity=27.0588235294118, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI19922422, Length=251, Percent_Identity=22.7091633466135, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 31108; Mature: 31108

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDHQPSIIPNTKRQDEVLWNVDEFGVATLTLNRTEKHNAFDACMIDLLIHRLDYLALRT
CCCCCCCCCCCCCCCHHHEECHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHC
DVRCLVLRGNGKHFSSGADLNWMKSMANSTKKQNLEDAQNLARLMHTLDAFPQPTIAVVQ
CCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEE
GSAFGGALGLICCCDIAVAAENAKFCLSEVKLGLVPATISPYVMRAMGNRQARRYILSAE
CCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHH
VINAAKAEKFGLVHEVHKLDKIEAVVESLIDSLLLNSPDAMRKAKALCHQCHQNPIDGQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHH
IQYTSQLIADIRVSPQGQDGLQAFLEKREPGWINKPAKGKK
HHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MQDHQPSIIPNTKRQDEVLWNVDEFGVATLTLNRTEKHNAFDACMIDLLIHRLDYLALRT
CCCCCCCCCCCCCCCHHHEECHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHC
DVRCLVLRGNGKHFSSGADLNWMKSMANSTKKQNLEDAQNLARLMHTLDAFPQPTIAVVQ
CCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEE
GSAFGGALGLICCCDIAVAAENAKFCLSEVKLGLVPATISPYVMRAMGNRQARRYILSAE
CCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHH
VINAAKAEKFGLVHEVHKLDKIEAVVESLIDSLLLNSPDAMRKAKALCHQCHQNPIDGQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHH
IQYTSQLIADIRVSPQGQDGLQAFLEKREPGWINKPAKGKK
HHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]