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The map label for this gene is dsbA [C]

Identifier: 156976617

GI number: 156976617

Start: 616275

End: 616907

Strand: Reverse

Name: dsbA [C]

Synonym: VIBHAR_05391

Alternate gene names: 156976617

Gene position: 616907-616275 (Counterclockwise)

Preceding gene: 156976619

Following gene: 156976616

Centisome position: 27.99

GC content: 44.55

Gene sequence:

>633_bases
ATGTTTAAAACTATTTCTAAAGGTGCAGCACTTTTTGCCGCAGTATTTGCCCTAACGGCTTGTGATAACGGCAATAGTCA
GCCACAACAAGGCAAACAATACGAAGTGTTGCCAGTCTCTCTTGCAGAGTACAACCTGGCTCCTGTTACTGAGGTGTTTT
CTCTGAACTGTGGTCACTGCCGTACCATGGAACAATTTGTGCCAAAAATCGAATCTTTGACAGAACAAAAAGTCGAGAAA
ATGCACGTAACCTTCAATGAGAGTGCACAAATCAGCGCAATCATTTTCTACACAGCAGCAATGCAGGTGAACGGTACGCC
TGACAAAGCGTTTATGGAAGAACTATTCGCGGCGGTTCAAATGGGAGCAGAAGCGACTGCTGATGAGCGTCAAGCAGCGG
TAGAGAAAGCATTCGAGTCTCGTAATCTAGTGAGCCCTTATCAACTAGACGATGCTCAACAAGCAAAACTGTTTGAATAC
ATCACTAAAGCAGAGCAGATCACGACTCGTGGTCAAATCAACTCAGTGCCTGCCTTCATCGTAAACGGTAAGTACCAAGT
GATCACAGGTGGTCATGAAACCGTTGAAGCAATGGCAGACACCATCAACTATCTGCTAAAACAACCAAAATAA

Upstream 100 bases:

>100_bases
TTCGATTATCTCACTTTAGTGTAACAAGCCGTATTAATGGTTTTCGTCGATACCGTTAACCGATAAACTAGCAATAATTC
TACTATTTAGAGCTTTACCC

Downstream 100 bases:

>100_bases
GACAAGGAGCTATTGTGTCTAAATTTCTGTTTCCTCTTATTATTTTTGTCCTAGCGGCATTCTTTATCTACCGTACTTGG
ACGAACCACAAAGTGGCAGA

Product: hypothetical protein

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: Disulfide Oxidoreductase; Lipoprotein

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MFKTISKGAALFAAVFALTACDNGNSQPQQGKQYEVLPVSLAEYNLAPVTEVFSLNCGHCRTMEQFVPKIESLTEQKVEK
MHVTFNESAQISAIIFYTAAMQVNGTPDKAFMEELFAAVQMGAEATADERQAAVEKAFESRNLVSPYQLDDAQQAKLFEY
ITKAEQITTRGQINSVPAFIVNGKYQVITGGHETVEAMADTINYLLKQPK

Sequences:

>Translated_210_residues
MFKTISKGAALFAAVFALTACDNGNSQPQQGKQYEVLPVSLAEYNLAPVTEVFSLNCGHCRTMEQFVPKIESLTEQKVEK
MHVTFNESAQISAIIFYTAAMQVNGTPDKAFMEELFAAVQMGAEATADERQAAVEKAFESRNLVSPYQLDDAQQAKLFEY
ITKAEQITTRGQINSVPAFIVNGKYQVITGGHETVEAMADTINYLLKQPK
>Mature_210_residues
MFKTISKGAALFAAVFALTACDNGNSQPQQGKQYEVLPVSLAEYNLAPVTEVFSLNCGHCRTMEQFVPKIESLTEQKVEK
MHVTFNESAQISAIIFYTAAMQVNGTPDKAFMEELFAAVQMGAEATADERQAAVEKAFESRNLVSPYQLDDAQQAKLFEY
ITKAEQITTRGQINSVPAFIVNGKYQVITGGHETVEAMADTINYLLKQPK

Specific function: Required For Disulfide Bond Formation In Some Periplasmic Proteins Such As Phoa Or Ompa. Acts By Transferring Its Disulfide Bond To Other Proteins And Is Reduced In The Process. Dsba Is Reoxidized By Dsbb. It Is Required For Pilus Biogenesis. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1348 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 23147; Mature: 23147

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00194 THIOREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKTISKGAALFAAVFALTACDNGNSQPQQGKQYEVLPVSLAEYNLAPVTEVFSLNCGHC
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEHHHHCCCCCHHHHHHCCCCCC
RTMEQFVPKIESLTEQKVEKMHVTFNESAQISAIIFYTAAMQVNGTPDKAFMEELFAAVQ
HHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEEEEEEEEECCCCCHHHHHHHHHHHH
MGAEATADERQAAVEKAFESRNLVSPYQLDDAQQAKLFEYITKAEQITTRGQINSVPAFI
HCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
VNGKYQVITGGHETVEAMADTINYLLKQPK
ECCEEEEEECCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFKTISKGAALFAAVFALTACDNGNSQPQQGKQYEVLPVSLAEYNLAPVTEVFSLNCGHC
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEHHHHCCCCCHHHHHHCCCCCC
RTMEQFVPKIESLTEQKVEKMHVTFNESAQISAIIFYTAAMQVNGTPDKAFMEELFAAVQ
HHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEEEEEEEEECCCCCHHHHHHHHHHHH
MGAEATADERQAAVEKAFESRNLVSPYQLDDAQQAKLFEYITKAEQITTRGQINSVPAFI
HCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
VNGKYQVITGGHETVEAMADTINYLLKQPK
ECCEEEEEECCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA