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The map label for this gene is phcA [H]

Identifier: 156976503

GI number: 156976503

Start: 520431

End: 521372

Strand: Direct

Name: phcA [H]

Synonym: VIBHAR_05276

Alternate gene names: 156976503

Gene position: 520431-521372 (Clockwise)

Preceding gene: 156976499

Following gene: 156976504

Centisome position: 23.61

GC content: 44.27

Gene sequence:

>942_bases
ATGGCCAAAGATCTCTTCTCCAACCTCGACCTCAATTTATTAAGAACGTTTATCATCCTCCACCAAGAACGAAACATGCG
CCGTGCGGCAGACCGGATGTTTGTGTCTCAACCTGCGGTGAGTAAAGCATTGCAGAGATTGCGTGATCACTTTGATGATG
AGCTATTTGTGAAAACCCATCATGGCTTGCGAGCTACCGAGTACGCGAACGATTTAGCGGAAAGCTTAGCGCCGTTACTT
GATGAGATTTCGATAACGTTAAATGGTTCAAAAGACTTTAACCCATTAGAGATGAACGGGACGGTCAAGATTGCGTTAGC
CCCTTTTATCCTTAGCTCCTATGCGAGCGAAATTTTCCAAGCAATCAGACAGGCCGCACCAAATGTGCAAATTCATTTAC
TGAACTGGTCGCGTTCTACCATGGATGAAATTATCAAGGATGAAGTGCTGCTGGGAGTGAACTACGAAATCAACCACTCC
CCCAAAGAACTCTTGACGAAAGAAATCGTAAAAGATGCGTTCTGTGCTTATGTTCGAAAAGATCATCCTTTGCAAGGTGA
TCGCTTACGAGTGTCAGACTGCCAGCATCTCGAGTTTGCTACCGTGATTGCTGCTGACTGGAACTCTAATATTTCATTTT
CAGAAAAAATAATGAAGATCAAAGGGGTAGAGCCTAAAATTGCGTTTCGCTCTGAGCTGCCATCTGCAATTATTGATGTG
GTTGCAAACTCAGATATGGTTTTTCCAGCATCAAAAACGGTCAATGTCGATAAGTTGTATAAGCTCCGTAGGCTGGATAT
TGAGCTCGACGGTATGGTGATCGAGCCATCACTGCTGGCTTACTACCACCATAAGAATCGACGTAATGATATGGTGCATT
GGTTGCGCAATATTGTAACGGAGATTATTACCGGGCACCTAGATCGAGATCGCCAACAATGA

Upstream 100 bases:

>100_bases
AGACGTATCGATCGCAAACATTTGGTGCCTTGCGGTTGGAAAGTCGTCAAATCTGTTCTTATGCTGAATAATAACCTTAA
GTTATCAACGACGTGAATTT

Downstream 100 bases:

>100_bases
TAACCCTCGGTTATCAAGCTATCTACCCTTGTTTATTAATAACCCGCCGTTAAGTACGGGTTTCTTTTTGGCGATTAGAG
TCATAAAACACTGTTCAATA

Product: transcriptional regulator

Products: NA

Alternate protein names: Virulence genes transcriptional regulator phcA [H]

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MAKDLFSNLDLNLLRTFIILHQERNMRRAADRMFVSQPAVSKALQRLRDHFDDELFVKTHHGLRATEYANDLAESLAPLL
DEISITLNGSKDFNPLEMNGTVKIALAPFILSSYASEIFQAIRQAAPNVQIHLLNWSRSTMDEIIKDEVLLGVNYEINHS
PKELLTKEIVKDAFCAYVRKDHPLQGDRLRVSDCQHLEFATVIAADWNSNISFSEKIMKIKGVEPKIAFRSELPSAIIDV
VANSDMVFPASKTVNVDKLYKLRRLDIELDGMVIEPSLLAYYHHKNRRNDMVHWLRNIVTEIITGHLDRDRQQ

Sequences:

>Translated_313_residues
MAKDLFSNLDLNLLRTFIILHQERNMRRAADRMFVSQPAVSKALQRLRDHFDDELFVKTHHGLRATEYANDLAESLAPLL
DEISITLNGSKDFNPLEMNGTVKIALAPFILSSYASEIFQAIRQAAPNVQIHLLNWSRSTMDEIIKDEVLLGVNYEINHS
PKELLTKEIVKDAFCAYVRKDHPLQGDRLRVSDCQHLEFATVIAADWNSNISFSEKIMKIKGVEPKIAFRSELPSAIIDV
VANSDMVFPASKTVNVDKLYKLRRLDIELDGMVIEPSLLAYYHHKNRRNDMVHWLRNIVTEIITGHLDRDRQQ
>Mature_312_residues
AKDLFSNLDLNLLRTFIILHQERNMRRAADRMFVSQPAVSKALQRLRDHFDDELFVKTHHGLRATEYANDLAESLAPLLD
EISITLNGSKDFNPLEMNGTVKIALAPFILSSYASEIFQAIRQAAPNVQIHLLNWSRSTMDEIIKDEVLLGVNYEINHSP
KELLTKEIVKDAFCAYVRKDHPLQGDRLRVSDCQHLEFATVIAADWNSNISFSEKIMKIKGVEPKIAFRSELPSAIIDVV
ANSDMVFPASKTVNVDKLYKLRRLDIELDGMVIEPSLLAYYHHKNRRNDMVHWLRNIVTEIITGHLDRDRQQ

Specific function: Regulates the transcription of one or more of the genes involved in virulence [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081684, Length=251, Percent_Identity=29.0836653386454, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790147, Length=173, Percent_Identity=31.2138728323699, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787601, Length=302, Percent_Identity=19.8675496688742, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: 825 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35931; Mature: 35800

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKDLFSNLDLNLLRTFIILHQERNMRRAADRMFVSQPAVSKALQRLRDHFDDELFVKTH
CCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEC
HGLRATEYANDLAESLAPLLDEISITLNGSKDFNPLEMNGTVKIALAPFILSSYASEIFQ
CCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCEEECCEEEEEHHHHHHHHHHHHHHH
AIRQAAPNVQIHLLNWSRSTMDEIIKDEVLLGVNYEINHSPKELLTKEIVKDAFCAYVRK
HHHHHCCCEEEEEECCCHHHHHHHHHHHEEEECCEEECCCHHHHHHHHHHHHHHHHHHHC
DHPLQGDRLRVSDCQHLEFATVIAADWNSNISFSEKIMKIKGVEPKIAFRSELPSAIIDV
CCCCCCCCEECCCCCCCEEEEEEEECCCCCCCHHHHHHHHCCCCCCHHHHHHCHHHHHHH
VANSDMVFPASKTVNVDKLYKLRRLDIELDGMVIEPSLLAYYHHKNRRNDMVHWLRNIVT
HCCCCEEECCCCCCCHHHHHHHHHCCEEECCEEECHHHHHHHHHCCCCHHHHHHHHHHHH
EIITGHLDRDRQQ
HHHHCCCCCCCCC
>Mature Secondary Structure 
AKDLFSNLDLNLLRTFIILHQERNMRRAADRMFVSQPAVSKALQRLRDHFDDELFVKTH
CCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEC
HGLRATEYANDLAESLAPLLDEISITLNGSKDFNPLEMNGTVKIALAPFILSSYASEIFQ
CCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCEEECCEEEEEHHHHHHHHHHHHHHH
AIRQAAPNVQIHLLNWSRSTMDEIIKDEVLLGVNYEINHSPKELLTKEIVKDAFCAYVRK
HHHHHCCCEEEEEECCCHHHHHHHHHHHEEEECCEEECCCHHHHHHHHHHHHHHHHHHHC
DHPLQGDRLRVSDCQHLEFATVIAADWNSNISFSEKIMKIKGVEPKIAFRSELPSAIIDV
CCCCCCCCEECCCCCCCEEEEEEEECCCCCCCHHHHHHHHCCCCCCHHHHHHCHHHHHHH
VANSDMVFPASKTVNVDKLYKLRRLDIELDGMVIEPSLLAYYHHKNRRNDMVHWLRNIVT
HCCCCEEECCCCCCCHHHHHHHHHCCEEECCEEECHHHHHHHHHCCCCHHHHHHHHHHHH
EIITGHLDRDRQQ
HHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8366033; 10913109; 11823852 [H]