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The map label for this gene is nemR [H]

Identifier: 156976422

GI number: 156976422

Start: 451421

End: 452008

Strand: Reverse

Name: nemR [H]

Synonym: VIBHAR_05195

Alternate gene names: 156976422

Gene position: 452008-451421 (Counterclockwise)

Preceding gene: 156976423

Following gene: 156976421

Centisome position: 20.51

GC content: 44.9

Gene sequence:

>588_bases
ATGAACGCAAAAACGAACGATACACGCCAACACATACTCGATGTCGGCTACCAATTGGTAGTGACCAAAGGTTTTACGAG
CGTTGGGCTCTCAGAACTTTTAAAGGCAGCGGATGTGCCGAAAGGCTCTTTCTACCATTACTTTAAGTCGAAAGAACAAT
TTGGTGAGGCATTGATTGAAGATTACTTCTCTCGCTACCTTAAGATGATCACAGCCCATTTTACTGAAAGTGAAGGCTCT
GGTCGCGACCGCCTTTTGAGCTACTTTGAGCGCTGGATGGCGATTGAAAATGGCAAATGTAACGCCAATAAATGCTTAGT
GGTGAAGTTGAGTGCTGAAGTCTCTGATCTTTCAGAAGCAATGCGCGCAAAACTGGCTTCGGGTGCCACTCATGTGGTGA
AAACCATCGAAGATTGTGTCCAAGCGGGCATTGAAGACGGCTCTATCCAAGTTGATTCAGCAGAGCAGACTGCCCACTTG
ATTTACCATCAATGGATTGGTGCAAGCTTACTCAACAAACTGTATCAGGATCGTTCCGGTTTAATTCGTAGCTTTGAGAG
CACTCAAAGTATGCTAAAAAGCAAATAG

Upstream 100 bases:

>100_bases
ACCCGAACATCAATGAGCTGTATGTGATTGAGTACGAGCTGATCAAGTAAGAACCATAAATCACTAGACGACTGGTCTAA
TCGCTTTTATAGTATTGCTC

Downstream 100 bases:

>100_bases
CGAACCACACTTAATCCGCCTTATCTTTACCCAAAAAGTACGGCGGATTTTTTAGGAATCAATACTAGACGACTGGTCTA
ATACTTAATATAGGAACTTA

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 195; Mature: 195

Protein sequence:

>195_residues
MNAKTNDTRQHILDVGYQLVVTKGFTSVGLSELLKAADVPKGSFYHYFKSKEQFGEALIEDYFSRYLKMITAHFTESEGS
GRDRLLSYFERWMAIENGKCNANKCLVVKLSAEVSDLSEAMRAKLASGATHVVKTIEDCVQAGIEDGSIQVDSAEQTAHL
IYHQWIGASLLNKLYQDRSGLIRSFESTQSMLKSK

Sequences:

>Translated_195_residues
MNAKTNDTRQHILDVGYQLVVTKGFTSVGLSELLKAADVPKGSFYHYFKSKEQFGEALIEDYFSRYLKMITAHFTESEGS
GRDRLLSYFERWMAIENGKCNANKCLVVKLSAEVSDLSEAMRAKLASGATHVVKTIEDCVQAGIEDGSIQVDSAEQTAHL
IYHQWIGASLLNKLYQDRSGLIRSFESTQSMLKSK
>Mature_195_residues
MNAKTNDTRQHILDVGYQLVVTKGFTSVGLSELLKAADVPKGSFYHYFKSKEQFGEALIEDYFSRYLKMITAHFTESEGS
GRDRLLSYFERWMAIENGKCNANKCLVVKLSAEVSDLSEAMRAKLASGATHVVKTIEDCVQAGIEDGSIQVDSAEQTAHL
IYHQWIGASLLNKLYQDRSGLIRSFESTQSMLKSK

Specific function: Represses the transcription of the nemRA operon by binding to the nemR box [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH tetR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI145693141, Length=194, Percent_Identity=41.7525773195876, Blast_Score=147, Evalue=4e-37,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR015893
- InterPro:   IPR011075
- InterPro:   IPR001647 [H]

Pfam domain/function: PF00440 TetR_N [H]

EC number: NA

Molecular weight: Translated: 21830; Mature: 21830

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS50977 HTH_TETR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAKTNDTRQHILDVGYQLVVTKGFTSVGLSELLKAADVPKGSFYHYFKSKEQFGEALIE
CCCCCCHHHHHHHHHHHHHEEECCCHHHCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
DYFSRYLKMITAHFTESEGSGRDRLLSYFERWMAIENGKCNANKCLVVKLSAEVSDLSEA
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHH
MRAKLASGATHVVKTIEDCVQAGIEDGSIQVDSAEQTAHLIYHQWIGASLLNKLYQDRSG
HHHHHHCCHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIRSFESTQSMLKSK
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MNAKTNDTRQHILDVGYQLVVTKGFTSVGLSELLKAADVPKGSFYHYFKSKEQFGEALIE
CCCCCCHHHHHHHHHHHHHEEECCCHHHCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
DYFSRYLKMITAHFTESEGSGRDRLLSYFERWMAIENGKCNANKCLVVKLSAEVSDLSEA
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHH
MRAKLASGATHVVKTIEDCVQAGIEDGSIQVDSAEQTAHLIYHQWIGASLLNKLYQDRSG
HHHHHHCCHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIRSFESTQSMLKSK
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]