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The map label for this gene is citH [H]

Identifier: 156936569

GI number: 156936569

Start: 20988

End: 22295

Strand: Direct

Name: citH [H]

Synonym: ESA_pESA3p05450

Alternate gene names: 156936569

Gene position: 20988-22295 (Clockwise)

Preceding gene: 156936567

Following gene: 156936570

Centisome position: 16.0

GC content: 60.24

Gene sequence:

>1308_bases
ATGCATTCCGCTACCTCACCTGTAAACACCCGTTCCCGGATCGGCGCTATTTTGCGCGTCACGTCCGGCAACTTTCTGGA
GCAGTTTGATTTTTTCCTGTTCGGCTTTTATGCCACCTACATCGCGCACACGTTCTTCCCGGCGAGCAGTGAGTTCGCCT
CGCTGATGATGACGTTTGCGGTGTTTGGCGCAGGCTTTCTGATGCGCCCCATCGGCGCGATAGTGCTGGGCGCGTACATC
GATAAAGTGGGCCGCCGCAAAGGGCTGATTATGACGCTCTCTATCATGGCGGCGGGCACCTTTCTGATTGTGCTGATCCC
GTCTTATGAGGCCATCGGGCTGTGGGCGCCGCTGTTCGTGCTGATTGGCCGCCTGTTACAGGGCTTTTCTGCCGGTGCGG
AGCTCGGCGGCGTGTCGGTGTATCTCGCCGAAATCGCGACGCCGGGGCGCAAAGGCTTTTACACCAGCTGGCAGTCAGGC
AGCCAGCAGGTCGCCATTATGGTGGCGGCGGCAATGGGCTTTGCCCTCAACGCGGCGCTGGATCAGTCGGCCATCAGCAG
CTGGGGCTGGCGGCTCCCGTTCCTCTTTGGTTGCCTGATTGTGCCGTTTATTTTCGTGCTGCGGCGCAAGCTTGAAGAGA
CGCAGGAGTTCAGCGCGCGCCGTCACCATCTCGACATGAAACAGGTGTTCCGCATGCTGCTGACCCACTGGCCGGTGGTT
GTCGCGGGCATGATGATGGTGGCAATGACCACCACGGCGTTTTATCTCATCACCGTGTATGCGCCCACCTTCGGCAAAAA
AGTGCTGATGCTGAGCGCCTCGGACAGCCTGCTGGTGACGCTGCTGGTGGCGATTTCCAACTTCTTCTGGCTGCCGGTGG
GTGGCGCGCTTTCTGACCGTTTCGGGCGTAAACCAGTGCTGATTGCCATGACGCTGCTGGCGCTGGCCACCGCGTATCCG
GCGCTGATGATGCTGGCCGCGTCACCTGGCTTTTCCATGATGCTGGGCGTGTTGCTGTGGCTCTCTTTTCTGTATGGCCT
GTACAACGGCGCGATGATCCCGGCGCTCACTGAAATCATGCCCGCCGAGGTGCGTGTCGCTGGTTTTTCGCTCGCCTACA
GCCTCGCGACGGCGATTTTCGGCGGCTTTACGCCGGTGATTTCCACGGCGCTGATTGAGTACACCGGCGACAAAGCCTCA
CCCGGCTACTGGATGAGCTTTGCCGCGCTCTGCGCGCTGCTGGCGACGCTCTACCTTTACCGCCGCAGTACCATGACATT
ACAGACCGCAGGACGCCGCCATGCATAA

Upstream 100 bases:

>100_bases
CATCGGACGATGCGTTTATTACGCTTATTACCTCGATATTTCTGTTCCAGTCTTCAGTAAGAGACGCAACCACAAGCGTT
AATGACAACTGGAGACATCA

Downstream 100 bases:

>100_bases
GAGAATGACGACATGGATAGCCGCGCTGACGCTCTTACCGTTAAGCAGTGTGGCGCTGGCACAGGAAATTACGGTGATGA
TTTCAGGCGGCTTTAAGGCG

Product: hypothetical protein

Products: Proton [Cytoplasm]; 2-oxoglutarate [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MHSATSPVNTRSRIGAILRVTSGNFLEQFDFFLFGFYATYIAHTFFPASSEFASLMMTFAVFGAGFLMRPIGAIVLGAYI
DKVGRRKGLIMTLSIMAAGTFLIVLIPSYEAIGLWAPLFVLIGRLLQGFSAGAELGGVSVYLAEIATPGRKGFYTSWQSG
SQQVAIMVAAAMGFALNAALDQSAISSWGWRLPFLFGCLIVPFIFVLRRKLEETQEFSARRHHLDMKQVFRMLLTHWPVV
VAGMMMVAMTTTAFYLITVYAPTFGKKVLMLSASDSLLVTLLVAISNFFWLPVGGALSDRFGRKPVLIAMTLLALATAYP
ALMMLAASPGFSMMLGVLLWLSFLYGLYNGAMIPALTEIMPAEVRVAGFSLAYSLATAIFGGFTPVISTALIEYTGDKAS
PGYWMSFAALCALLATLYLYRRSTMTLQTAGRRHA

Sequences:

>Translated_435_residues
MHSATSPVNTRSRIGAILRVTSGNFLEQFDFFLFGFYATYIAHTFFPASSEFASLMMTFAVFGAGFLMRPIGAIVLGAYI
DKVGRRKGLIMTLSIMAAGTFLIVLIPSYEAIGLWAPLFVLIGRLLQGFSAGAELGGVSVYLAEIATPGRKGFYTSWQSG
SQQVAIMVAAAMGFALNAALDQSAISSWGWRLPFLFGCLIVPFIFVLRRKLEETQEFSARRHHLDMKQVFRMLLTHWPVV
VAGMMMVAMTTTAFYLITVYAPTFGKKVLMLSASDSLLVTLLVAISNFFWLPVGGALSDRFGRKPVLIAMTLLALATAYP
ALMMLAASPGFSMMLGVLLWLSFLYGLYNGAMIPALTEIMPAEVRVAGFSLAYSLATAIFGGFTPVISTALIEYTGDKAS
PGYWMSFAALCALLATLYLYRRSTMTLQTAGRRHA
>Mature_435_residues
MHSATSPVNTRSRIGAILRVTSGNFLEQFDFFLFGFYATYIAHTFFPASSEFASLMMTFAVFGAGFLMRPIGAIVLGAYI
DKVGRRKGLIMTLSIMAAGTFLIVLIPSYEAIGLWAPLFVLIGRLLQGFSAGAELGGVSVYLAEIATPGRKGFYTSWQSG
SQQVAIMVAAAMGFALNAALDQSAISSWGWRLPFLFGCLIVPFIFVLRRKLEETQEFSARRHHLDMKQVFRMLLTHWPVV
VAGMMMVAMTTTAFYLITVYAPTFGKKVLMLSASDSLLVTLLVAISNFFWLPVGGALSDRFGRKPVLIAMTLLALATAYP
ALMMLAASPGFSMMLGVLLWLSFLYGLYNGAMIPALTEIMPAEVRVAGFSLAYSLATAIFGGFTPVISTALIEYTGDKAS
PGYWMSFAALCALLATLYLYRRSTMTLQTAGRRHA

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788942, Length=434, Percent_Identity=33.8709677419355, Blast_Score=205, Evalue=5e-54,
Organism=Escherichia coli, GI1790550, Length=426, Percent_Identity=28.8732394366197, Blast_Score=204, Evalue=9e-54,
Organism=Escherichia coli, GI1788292, Length=378, Percent_Identity=30.4232804232804, Blast_Score=187, Evalue=2e-48,
Organism=Escherichia coli, GI1789941, Length=390, Percent_Identity=30.5128205128205, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI87082231, Length=202, Percent_Identity=25.2475247524752, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47410; Mature: 47410

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSATSPVNTRSRIGAILRVTSGNFLEQFDFFLFGFYATYIAHTFFPASSEFASLMMTFA
CCCCCCCCCHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
VFGAGFLMRPIGAIVLGAYIDKVGRRKGLIMTLSIMAAGTFLIVLIPSYEAIGLWAPLFV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHCCEEEEEECCCCHHHHHHHHHH
LIGRLLQGFSAGAELGGVSVYLAEIATPGRKGFYTSWQSGSQQVAIMVAAAMGFALNAAL
HHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
DQSAISSWGWRLPFLFGCLIVPFIFVLRRKLEETQEFSARRHHLDMKQVFRMLLTHWPVV
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
VAGMMMVAMTTTAFYLITVYAPTFGKKVLMLSASDSLLVTLLVAISNFFWLPVGGALSDR
HHHHHHHHHHHHHHHHHHEECCCCCCEEEEEECCCHHHHHHHHHHHHHHEECCCCHHHHH
FGRKPVLIAMTLLALATAYPALMMLAASPGFSMMLGVLLWLSFLYGLYNGAMIPALTEIM
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHC
PAEVRVAGFSLAYSLATAIFGGFTPVISTALIEYTGDKASPGYWMSFAALCALLATLYLY
CHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
RRSTMTLQTAGRRHA
HHCCCEEECCCCCCC
>Mature Secondary Structure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HHCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 2-oxoglutarate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 2-oxoglutarate [Periplasm] = Proton [Cytoplasm] + 2-oxoglutarate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]