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The map label for this gene is rnhB [H]

Identifier: 156938224

GI number: 156938224

Start: 1293281

End: 1293928

Strand: Reverse

Name: rnhB [H]

Synonym: Igni_1437

Alternate gene names: 156938224

Gene position: 1293928-1293281 (Counterclockwise)

Preceding gene: 156938227

Following gene: 156938223

Centisome position: 99.72

GC content: 51.7

Gene sequence:

>648_bases
TTGAGAACGATGATAGTGGCCGGGATAGACGAGGCCGGGCGCGGACCGGTCATAGGGAGCATGTTCGTCGCATTGGTTGC
CATCGAACAAGATCAATTAGAGAAATTGGTGAGCTTGGGCTTGAAAGAAAGCAAAATGTTGACTCCTAACAAAAGGAGGT
ACTTTTACAACTTGATAATGAAGACTGCAAAGCTTGTATTGATAAAGGAAGTGAAAGCTGAAGAAATAGACAAGAGGAAC
TTGAGCGAGCTTACTATAGAAGTTATGAAAGAACTGTTGGAGACCGCTCTAAACAAGGTAAAGATAGACGTAGTCTGCGT
CGATATCGTTGGAAACGGCGCCAAGCAGAGGCGGGCGCTCGGCGAGGTGTTCCCGGACGTTATCGTAATGAAGAAGGGAG
ACGCCTCGTGTCCGGCAGCGGCAGCGGCGTCGATAGTGGCGAAGGAAGCGAGGGAGAGGCACGTCAGCCAGCTTAGAGCC
CGTTACGGGGACTTCGGTTCCGGCTACCCTTCCGACCCCAAGACGCTTAGGTGGCTTAGCGTCACGCGACCCCTTCCCCC
AATAGTCAGGAAGAAGTGGAAGACGATCAGAAGAAAAGGGATGGGTGTGGGAGGGGAGGGGGAACGGAAGCATGATAATA
GTGCTTAA

Upstream 100 bases:

>100_bases
CTCTGCGCGAGGGGCGAAAACAAAAGGTCTCCCAACCCGTTTGTTGACGTTGCTTCTTTAACGACGGGTAACGTAGTTAG
ACGCTCTTTGCCTAACTTTC

Downstream 100 bases:

>100_bases
ACAAGGCGAGGGCGGATCGGAAGTCAAGAACGTCTTACAAGAGAAGGGCTTGAAGTTCTGGGAAGTCACCTTATACGGCC
GTCCTCTGATAGTGACCCCT

Product: RNase HII

Products: NA

Alternate protein names: RNase HII [H]

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MRTMIVAGIDEAGRGPVIGSMFVALVAIEQDQLEKLVSLGLKESKMLTPNKRRYFYNLIMKTAKLVLIKEVKAEEIDKRN
LSELTIEVMKELLETALNKVKIDVVCVDIVGNGAKQRRALGEVFPDVIVMKKGDASCPAAAAASIVAKEARERHVSQLRA
RYGDFGSGYPSDPKTLRWLSVTRPLPPIVRKKWKTIRRKGMGVGGEGERKHDNSA

Sequences:

>Translated_215_residues
MRTMIVAGIDEAGRGPVIGSMFVALVAIEQDQLEKLVSLGLKESKMLTPNKRRYFYNLIMKTAKLVLIKEVKAEEIDKRN
LSELTIEVMKELLETALNKVKIDVVCVDIVGNGAKQRRALGEVFPDVIVMKKGDASCPAAAAASIVAKEARERHVSQLRA
RYGDFGSGYPSDPKTLRWLSVTRPLPPIVRKKWKTIRRKGMGVGGEGERKHDNSA
>Mature_215_residues
MRTMIVAGIDEAGRGPVIGSMFVALVAIEQDQLEKLVSLGLKESKMLTPNKRRYFYNLIMKTAKLVLIKEVKAEEIDKRN
LSELTIEVMKELLETALNKVKIDVVCVDIVGNGAKQRRALGEVFPDVIVMKKGDASCPAAAAASIVAKEARERHVSQLRA
RYGDFGSGYPSDPKTLRWLSVTRPLPPIVRKKWKTIRRKGMGVGGEGERKHDNSA

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family [H]

Homologues:

Organism=Homo sapiens, GI38455391, Length=212, Percent_Identity=33.9622641509434, Blast_Score=94, Evalue=7e-20,
Organism=Escherichia coli, GI1786380, Length=174, Percent_Identity=30.4597701149425, Blast_Score=60, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17536263, Length=217, Percent_Identity=33.6405529953917, Blast_Score=88, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6324257, Length=197, Percent_Identity=34.5177664974619, Blast_Score=71, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24580635, Length=213, Percent_Identity=32.3943661971831, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004649
- InterPro:   IPR001352
- InterPro:   IPR020787
- InterPro:   IPR023160
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01351 RNase_HII [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 23828; Mature: 23828

Theoretical pI: Translated: 10.47; Mature: 10.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTMIVAGIDEAGRGPVIGSMFVALVAIEQDQLEKLVSLGLKESKMLTPNKRRYFYNLIM
CCCEEEECCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
KTAKLVLIKEVKAEEIDKRNLSELTIEVMKELLETALNKVKIDVVCVDIVGNGAKQRRAL
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCHHHHHHH
GEVFPDVIVMKKGDASCPAAAAASIVAKEARERHVSQLRARYGDFGSGYPSDPKTLRWLS
HHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEE
VTRPLPPIVRKKWKTIRRKGMGVGGEGERKHDNSA
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRTMIVAGIDEAGRGPVIGSMFVALVAIEQDQLEKLVSLGLKESKMLTPNKRRYFYNLIM
CCCEEEECCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
KTAKLVLIKEVKAEEIDKRNLSELTIEVMKELLETALNKVKIDVVCVDIVGNGAKQRRAL
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCHHHHHHH
GEVFPDVIVMKKGDASCPAAAAASIVAKEARERHVSQLRARYGDFGSGYPSDPKTLRWLS
HHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEE
VTRPLPPIVRKKWKTIRRKGMGVGGEGERKHDNSA
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572479 [H]