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The map label for this gene is hydB [H]

Identifier: 156938154

GI number: 156938154

Start: 1222253

End: 1224247

Strand: Direct

Name: hydB [H]

Synonym: Igni_1367

Alternate gene names: 156938154

Gene position: 1222253-1224247 (Clockwise)

Preceding gene: 156938153

Following gene: 156938155

Centisome position: 94.2

GC content: 58.7

Gene sequence:

>1995_bases
ATGTCCGAGAAGGTTAACCGTCCGGTAAGGAAGGAGGAGAAGAAGGACCCCAAGATGGTCCTCCTAGCCCCTAACGTCAA
GAGGATAAAGGGCAACACTGCCGAGATATTCATTGACCCCATAACGAGGATCGAAGGCCACCTAGGCCTCAAGGCAGAGG
TTGACGTAACCAGCAGGAAGACCACCAACACTTGGGTGTTCGCCACCATGTTCAGAGGCTTCGAAGTGTTCATGAGGGGG
AGGCCGCCTGAGGACGCCATCGCGATATCCTCTAGGGTCTGCGGCGTTTGCGGCGCCAGCCACGCCAACGCGGCCACTAT
GGCGACCGACATGGCGCTGGGCGCCGTGCCCACTCCCTTAGGAGTGGTCCTCAGGAACATGGCTTTCGTGATGACCGACT
TGCTATATGATCACGCTATAATACTTAACTTGCTCGGAGGGCCGGACTACAGCGAGGTCGCGGTCAGCAAGCTCACCCCC
AGCTGCTGGGAGGCCGCCAAGACCACGCCGGCCGAGTACTCCGCGGTCCACGGCTACAGGACGATAGCCGACATAATGAA
GGATATGAACCCGATAACCGGGAGGATATGGCAGCTCACCGTGAAGTATCAGAGGATAGCGAGAGAGGCCGGCGTGCTCA
TTTACGGAAGGCACTCCCACCCCGCCACTTTGATACCCGGCGGCATAAGCACCGACTTGAGCACCGCTCAGTACCTCTTC
GAGGGCTACGCCTTCCGCCTGACCCAACTGACCGCTTGGACCAAGTTCGTGGTAGCTGTGTGGATGGATTTGGCAGACTT
CTATAACAAGAACTGCAACTACGAGGACGAGGGTTACACGTACTCGAAGCCTATACTCTACTCCTCCGGCGTCTTCGACG
ACCCCGAGGCTTACAGCCAGCTCGGCGACGATTACAAGACCATATATGAGAAGATAGACGAAGTGGCCAAGAAGAGGGCA
GCGCCGCCAGGGCTCTACATAGGAGGGGAGTTGGTAACCGAGAACATCACGGAGCACCAGAGGAACGTAGTAGAGCTCGT
GGACAGCTCCTTCTACGAGGAATGGAAAGACAAGACCACCCTCTTCACTAAGACCGACCCGGCGGGCAACCCGCTGGTGG
GAGGCGACGAGACCCTAGTGTGGTACCACCCGTGGAACAAGACCACCATCCCGAGGCCCACGGACAGCAGTTGGAGCGGC
AAGTACAGCTGGGCGGCCACCGTAAGGCTGTACTGGAAAGGGAAGCTATACCCGTTCGAAGTCGGCCCGATAGCCAAGAT
GTACGTAATGGCCCACAGCAACTTGAAGGGTTGGGTCGAGGAGGAAGGGGCGCCCGTCAAGCACGGCGGCGGCAAGATAG
AGGTGACCTTGCCGGCCTCCCGCAACGTGACCGACCTGCCTAAGGGCACTTGGGAAGAGGTGACCTTCACCTACTACGCA
CCCCCCAAGAGCACTACCATAGAGAGGGTCAGGGCGAGGGCCTTCGACATGGCGGTCGACGTAGCCGACGCGTGGGCGTT
CGTACTGAGGGGGCTGTACTACATCAACAGCAAGGGAATGACCCAAGCCTCCAGGCCTTGGAAGGAGCCGGCCTTCTCGC
TGGGCTTCGGCCAGCTAGAGGTGCCCAGAGGGAACGTGGAGCACTGGATAGTGGTGAAGAACGGGAGAATAGCGAACTAC
CAGATCCACGCGCCCACTACCCAGAACGTAGGTCCGAGGGCCCCCAAGGGAGAGCTAGCTAGGTACTGTGCCAACGAGCC
TTGGGTCACCAAGAGCCCAGAGGGCTACTGCCTAAGCCCGTTCGAGGCCGCGGCGCTCCACACCGTAGTGAGTGAGGAGG
TGGAGCCGGAGAAGTGGACCGGCTTGGACTACGTGAGGGCGATAAGGAGCTTCGACCCGTGCATAGCTTGTGCCGCTCAC
ATAGAGTTCGTGAAGAACAAGAAGGTGCTAAAGAGCGTTAAGAAGCTACTCACCAACGCGTGTTCCATACAATAA

Upstream 100 bases:

>100_bases
CGCCCGAGGCCTTGGCCACCGCGGTCGGAGCGGCCGCGATAGGTGCGGTGGTAGGATACGCGCTTACTAAAAAGGCAAAG
GGGTAAGGGGGTGAGGGAGC

Downstream 100 bases:

>100_bases
GCTTTTTGTTTACCTAGTGGAAAGTAGACCGTTTTAAGGTCAAGTTTGACCGGAAGGTGAGGAGCCATGACGGTCGGAGG
CTGGGGATCGCCCAGCGACC

Product: nickel-dependent hydrogenase, large subunit

Products: NA

Alternate protein names: NiFe hydrogenlyase large chain [H]

Number of amino acids: Translated: 664; Mature: 663

Protein sequence:

>664_residues
MSEKVNRPVRKEEKKDPKMVLLAPNVKRIKGNTAEIFIDPITRIEGHLGLKAEVDVTSRKTTNTWVFATMFRGFEVFMRG
RPPEDAIAISSRVCGVCGASHANAATMATDMALGAVPTPLGVVLRNMAFVMTDLLYDHAIILNLLGGPDYSEVAVSKLTP
SCWEAAKTTPAEYSAVHGYRTIADIMKDMNPITGRIWQLTVKYQRIAREAGVLIYGRHSHPATLIPGGISTDLSTAQYLF
EGYAFRLTQLTAWTKFVVAVWMDLADFYNKNCNYEDEGYTYSKPILYSSGVFDDPEAYSQLGDDYKTIYEKIDEVAKKRA
APPGLYIGGELVTENITEHQRNVVELVDSSFYEEWKDKTTLFTKTDPAGNPLVGGDETLVWYHPWNKTTIPRPTDSSWSG
KYSWAATVRLYWKGKLYPFEVGPIAKMYVMAHSNLKGWVEEEGAPVKHGGGKIEVTLPASRNVTDLPKGTWEEVTFTYYA
PPKSTTIERVRARAFDMAVDVADAWAFVLRGLYYINSKGMTQASRPWKEPAFSLGFGQLEVPRGNVEHWIVVKNGRIANY
QIHAPTTQNVGPRAPKGELARYCANEPWVTKSPEGYCLSPFEAAALHTVVSEEVEPEKWTGLDYVRAIRSFDPCIACAAH
IEFVKNKKVLKSVKKLLTNACSIQ

Sequences:

>Translated_664_residues
MSEKVNRPVRKEEKKDPKMVLLAPNVKRIKGNTAEIFIDPITRIEGHLGLKAEVDVTSRKTTNTWVFATMFRGFEVFMRG
RPPEDAIAISSRVCGVCGASHANAATMATDMALGAVPTPLGVVLRNMAFVMTDLLYDHAIILNLLGGPDYSEVAVSKLTP
SCWEAAKTTPAEYSAVHGYRTIADIMKDMNPITGRIWQLTVKYQRIAREAGVLIYGRHSHPATLIPGGISTDLSTAQYLF
EGYAFRLTQLTAWTKFVVAVWMDLADFYNKNCNYEDEGYTYSKPILYSSGVFDDPEAYSQLGDDYKTIYEKIDEVAKKRA
APPGLYIGGELVTENITEHQRNVVELVDSSFYEEWKDKTTLFTKTDPAGNPLVGGDETLVWYHPWNKTTIPRPTDSSWSG
KYSWAATVRLYWKGKLYPFEVGPIAKMYVMAHSNLKGWVEEEGAPVKHGGGKIEVTLPASRNVTDLPKGTWEEVTFTYYA
PPKSTTIERVRARAFDMAVDVADAWAFVLRGLYYINSKGMTQASRPWKEPAFSLGFGQLEVPRGNVEHWIVVKNGRIANY
QIHAPTTQNVGPRAPKGELARYCANEPWVTKSPEGYCLSPFEAAALHTVVSEEVEPEKWTGLDYVRAIRSFDPCIACAAH
IEFVKNKKVLKSVKKLLTNACSIQ
>Mature_663_residues
SEKVNRPVRKEEKKDPKMVLLAPNVKRIKGNTAEIFIDPITRIEGHLGLKAEVDVTSRKTTNTWVFATMFRGFEVFMRGR
PPEDAIAISSRVCGVCGASHANAATMATDMALGAVPTPLGVVLRNMAFVMTDLLYDHAIILNLLGGPDYSEVAVSKLTPS
CWEAAKTTPAEYSAVHGYRTIADIMKDMNPITGRIWQLTVKYQRIAREAGVLIYGRHSHPATLIPGGISTDLSTAQYLFE
GYAFRLTQLTAWTKFVVAVWMDLADFYNKNCNYEDEGYTYSKPILYSSGVFDDPEAYSQLGDDYKTIYEKIDEVAKKRAA
PPGLYIGGELVTENITEHQRNVVELVDSSFYEEWKDKTTLFTKTDPAGNPLVGGDETLVWYHPWNKTTIPRPTDSSWSGK
YSWAATVRLYWKGKLYPFEVGPIAKMYVMAHSNLKGWVEEEGAPVKHGGGKIEVTLPASRNVTDLPKGTWEEVTFTYYAP
PKSTTIERVRARAFDMAVDVADAWAFVLRGLYYINSKGMTQASRPWKEPAFSLGFGQLEVPRGNVEHWIVVKNGRIANYQ
IHAPTTQNVGPRAPKGELARYCANEPWVTKSPEGYCLSPFEAAALHTVVSEEVEPEKWTGLDYVRAIRSFDPCIACAAHI
EFVKNKKVLKSVKKLLTNACSIQ

Specific function: Catalyzes the reversible oxidoreduction of molecular hydrogen, in conjunction with a specific electron acceptor, cytochrome c3 [H]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787207, Length=651, Percent_Identity=25.0384024577573, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1789368, Length=643, Percent_Identity=25.194401244168, Blast_Score=128, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.2.1 [H]

Molecular weight: Translated: 74194; Mature: 74063

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKVNRPVRKEEKKDPKMVLLAPNVKRIKGNTAEIFIDPITRIEGHLGLKAEVDVTSRK
CCCCCCCCHHHHHCCCCCEEEECCCCCEECCCCEEEEECCHHHHCCCCCCEEEEEECCCC
TTNTWVFATMFRGFEVFMRGRPPEDAIAISSRVCGVCGASHANAATMATDMALGAVPTPL
CCCCEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCH
GVVLRNMAFVMTDLLYDHAIILNLLGGPDYSEVAVSKLTPSCWEAAKTTPAEYSAVHGYR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHH
TIADIMKDMNPITGRIWQLTVKYQRIAREAGVLIYGRHSHPATLIPGGISTDLSTAQYLF
HHHHHHHHCCCCCCEEEEEEHHHHHHHHHCCEEEEECCCCCCEEECCCCCCCHHHHHHHH
EGYAFRLTQLTAWTKFVVAVWMDLADFYNKNCNYEDEGYTYSKPILYSSGVFDDPEAYSQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCEEEECCCCCCHHHHHH
LGDDYKTIYEKIDEVAKKRAAPPGLYIGGELVTENITEHQRNVVELVDSSFYEEWKDKTT
HCCHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LFTKTDPAGNPLVGGDETLVWYHPWNKTTIPRPTDSSWSGKYSWAATVRLYWKGKLYPFE
EEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEEEEEEEECCEEEEE
VGPIAKMYVMAHSNLKGWVEEEGAPVKHGGGKIEVTLPASRNVTDLPKGTWEEVTFTYYA
CCCCEEEEEEECCCCCCHHHCCCCCEECCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEC
PPKSTTIERVRARAFDMAVDVADAWAFVLRGLYYINSKGMTQASRPWKEPAFSLGFGQLE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCEEE
VPRGNVEHWIVVKNGRIANYQIHAPTTQNVGPRAPKGELARYCANEPWVTKSPEGYCLSP
CCCCCCCEEEEEECCEEEEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCEECC
FEAAALHTVVSEEVEPEKWTGLDYVRAIRSFDPCIACAAHIEFVKNKKVLKSVKKLLTNA
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CSIQ
HCCC
>Mature Secondary Structure 
SEKVNRPVRKEEKKDPKMVLLAPNVKRIKGNTAEIFIDPITRIEGHLGLKAEVDVTSRK
CCCCCCCHHHHHCCCCCEEEECCCCCEECCCCEEEEECCHHHHCCCCCCEEEEEECCCC
TTNTWVFATMFRGFEVFMRGRPPEDAIAISSRVCGVCGASHANAATMATDMALGAVPTPL
CCCCEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCH
GVVLRNMAFVMTDLLYDHAIILNLLGGPDYSEVAVSKLTPSCWEAAKTTPAEYSAVHGYR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHH
TIADIMKDMNPITGRIWQLTVKYQRIAREAGVLIYGRHSHPATLIPGGISTDLSTAQYLF
HHHHHHHHCCCCCCEEEEEEHHHHHHHHHCCEEEEECCCCCCEEECCCCCCCHHHHHHHH
EGYAFRLTQLTAWTKFVVAVWMDLADFYNKNCNYEDEGYTYSKPILYSSGVFDDPEAYSQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCEEEECCCCCCHHHHHH
LGDDYKTIYEKIDEVAKKRAAPPGLYIGGELVTENITEHQRNVVELVDSSFYEEWKDKTT
HCCHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LFTKTDPAGNPLVGGDETLVWYHPWNKTTIPRPTDSSWSGKYSWAATVRLYWKGKLYPFE
EEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEEEEEEEECCEEEEE
VGPIAKMYVMAHSNLKGWVEEEGAPVKHGGGKIEVTLPASRNVTDLPKGTWEEVTFTYYA
CCCCEEEEEEECCCCCCHHHCCCCCEECCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEC
PPKSTTIERVRARAFDMAVDVADAWAFVLRGLYYINSKGMTQASRPWKEPAFSLGFGQLE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCEEE
VPRGNVEHWIVVKNGRIANYQIHAPTTQNVGPRAPKGELARYCANEPWVTKSPEGYCLSP
CCCCCCCEEEEEECCEEEEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCEECC
FEAAALHTVVSEEVEPEKWTGLDYVRAIRSFDPCIACAAHIEFVKNKKVLKSVKKLLTNA
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CSIQ
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2269874; 9438867 [H]