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The map label for this gene is hyb0 [C]

Identifier: 156938153

GI number: 156938153

Start: 1220970

End: 1222238

Strand: Direct

Name: hyb0 [C]

Synonym: Igni_1366

Alternate gene names: 156938153

Gene position: 1220970-1222238 (Clockwise)

Preceding gene: 156938152

Following gene: 156938154

Centisome position: 94.1

GC content: 61.7

Gene sequence:

>1269_bases
ATGAGCGTCACCAAACCTCGTAGGGAGTTCCTCAAGAAGTTGGGAGCCAGCACCTTACTGTTGGGGGCACTGAGCAACAT
AGACTGGGCAAACGCTATGGAGATGTTTAAGGAAGCCGTTAGGAAGGGCAAGGTTAACATAGTCTGGTTCGAGGCCCAGA
GCTGCACGGGCGACACCACCTCCTTGATCCAAGCCACCGACCCGGACTTGGTAGAGGTCTTAACCGGAGCTATCCACCTA
GCCGGACCCGGCACGGTGGGGCTCGCCTTCCACGACACCGTAATGCCCGAGTGGGGAGAGGGAGCTATAGAGATACTCAA
GCTCGCCGAGAGCGGCGCCTACGACCCCTTCGTGTTGGTCATAGAGGGTTCCATACCCGACGAGAACCACGCGAGGAAGT
TCTGCAAGGACCCCAACCGCTGCGGCTACTACTGCTTCGTGGGCGAGGAGAACGGGCGAGTGGTGACTTGTACCGAGTGG
ATACATAGGTTAGCTAAGAGGGCAGTCGCCGTAGTCGCCGCTGGCACGTGCGCGTCCTACGGAGGCATACCCTCCAACAA
GGTCCTCGAGCCCCCTCCGGGCTGGAGCTACCCCGAATGGAGCTTAGCCCCCACCGGCGCTGTAGGCTTCTTCGACGACC
CGGTCAAGGGCTACAAGGGCTTGATAAGCCGCCACGCGGAGCTGCCGAACCTCACCCCGTACTTCAACTACATCTTCAGA
GATTGTGAGCCCGAGCTAAGCCCCGGGAGCACTTGTAAGCCCGCCGTAGCAGTGCCCGGTTGTCCGACCAACGGCAACGG
CATACTAAGGGTGCTAGTACACCTAGTGTTGGCCGCGCTCTACCCGGACGTGGTACCCTTGCCTAGGGCTTCCGAGTGGC
TAGACCAGTACGCGAGGCCGAAGTACTTGTTCGGTCGCACGGTGCACGAGCAGTGTCCTAGGGCTGGCTACTACGCTGCC
GGGGACTTGAGGAGGTTGCCGGGCGACAACGACGCGAAGTGTCTGTTCGCGGTAGGGTGCAAGGGCCCGGCAGCGAACTG
TCCTTGGAACAAGGTCGGCTGGGTTAACGGCGTGGGCGGCCCCACGAGAACCGGAGGCGTGTGTATAGGCTGTACCATGC
CGGGCTTCAGCGACGCCTTCGAGCCCTTCTACAAGCCACTCCCGGCCCCGGCCGCTCCCAGCGCGCCCGAGGCCTTGGCC
ACCGCGGTCGGAGCGGCCGCGATAGGTGCGGTGGTAGGATACGCGCTTACTAAAAAGGCAAAGGGGTAA

Upstream 100 bases:

>100_bases
TAGTTTCGGCTAAGTAATTTACCGACCGGTAAAAGAAAGGGTATATAGGTTATTTACCAGCCGAGAATGTGGGGCAGAGC
CTCAAGAGTGTGGTGAGAGC

Downstream 100 bases:

>100_bases
GGGGGTGAGGGAGCATGTCCGAGAAGGTTAACCGTCCGGTAAGGAAGGAGGAGAAGAAGGACCCCAAGATGGTCCTCCTA
GCCCCTAACGTCAAGAGGAT

Product: nickel-dependent hydrogenase small subunit

Products: NA

Alternate protein names: NiFeSe hydrogenlyase small chain [H]

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MSVTKPRREFLKKLGASTLLLGALSNIDWANAMEMFKEAVRKGKVNIVWFEAQSCTGDTTSLIQATDPDLVEVLTGAIHL
AGPGTVGLAFHDTVMPEWGEGAIEILKLAESGAYDPFVLVIEGSIPDENHARKFCKDPNRCGYYCFVGEENGRVVTCTEW
IHRLAKRAVAVVAAGTCASYGGIPSNKVLEPPPGWSYPEWSLAPTGAVGFFDDPVKGYKGLISRHAELPNLTPYFNYIFR
DCEPELSPGSTCKPAVAVPGCPTNGNGILRVLVHLVLAALYPDVVPLPRASEWLDQYARPKYLFGRTVHEQCPRAGYYAA
GDLRRLPGDNDAKCLFAVGCKGPAANCPWNKVGWVNGVGGPTRTGGVCIGCTMPGFSDAFEPFYKPLPAPAAPSAPEALA
TAVGAAAIGAVVGYALTKKAKG

Sequences:

>Translated_422_residues
MSVTKPRREFLKKLGASTLLLGALSNIDWANAMEMFKEAVRKGKVNIVWFEAQSCTGDTTSLIQATDPDLVEVLTGAIHL
AGPGTVGLAFHDTVMPEWGEGAIEILKLAESGAYDPFVLVIEGSIPDENHARKFCKDPNRCGYYCFVGEENGRVVTCTEW
IHRLAKRAVAVVAAGTCASYGGIPSNKVLEPPPGWSYPEWSLAPTGAVGFFDDPVKGYKGLISRHAELPNLTPYFNYIFR
DCEPELSPGSTCKPAVAVPGCPTNGNGILRVLVHLVLAALYPDVVPLPRASEWLDQYARPKYLFGRTVHEQCPRAGYYAA
GDLRRLPGDNDAKCLFAVGCKGPAANCPWNKVGWVNGVGGPTRTGGVCIGCTMPGFSDAFEPFYKPLPAPAAPSAPEALA
TAVGAAAIGAVVGYALTKKAKG
>Mature_421_residues
SVTKPRREFLKKLGASTLLLGALSNIDWANAMEMFKEAVRKGKVNIVWFEAQSCTGDTTSLIQATDPDLVEVLTGAIHLA
GPGTVGLAFHDTVMPEWGEGAIEILKLAESGAYDPFVLVIEGSIPDENHARKFCKDPNRCGYYCFVGEENGRVVTCTEWI
HRLAKRAVAVVAAGTCASYGGIPSNKVLEPPPGWSYPEWSLAPTGAVGFFDDPVKGYKGLISRHAELPNLTPYFNYIFRD
CEPELSPGSTCKPAVAVPGCPTNGNGILRVLVHLVLAALYPDVVPLPRASEWLDQYARPKYLFGRTVHEQCPRAGYYAAG
DLRRLPGDNDAKCLFAVGCKGPAANCPWNKVGWVNGVGGPTRTGGVCIGCTMPGFSDAFEPFYKPLPAPAAPSAPEALAT
AVGAAAIGAVVGYALTKKAKG

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd2 Is Involved In Hydrogen Uptake. [C]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789371, Length=412, Percent_Identity=25.4854368932039, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1787206, Length=408, Percent_Identity=25, Blast_Score=95, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 44988; Mature: 44857

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVTKPRREFLKKLGASTLLLGALSNIDWANAMEMFKEAVRKGKVNIVWFEAQSCTGDTT
CCCCHHHHHHHHHHCCHHEEHHHHCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCH
SLIQATDPDLVEVLTGAIHLAGPGTVGLAFHDTVMPEWGEGAIEILKLAESGAYDPFVLV
HHEECCCCHHHHHHHHHEEECCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCEEEE
IEGSIPDENHARKFCKDPNRCGYYCFVGEENGRVVTCTEWIHRLAKRAVAVVAAGTCASY
EECCCCCHHHHHHHHCCCCCCEEEEEEECCCCCEEEHHHHHHHHHHHHHHHHCCCHHHHC
GGIPSNKVLEPPPGWSYPEWSLAPTGAVGFFDDPVKGYKGLISRHAELPNLTPYFNYIFR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHH
DCEPELSPGSTCKPAVAVPGCPTNGNGILRVLVHLVLAALYPDVVPLPRASEWLDQYARP
CCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCC
KYLFGRTVHEQCPRAGYYAAGDLRRLPGDNDAKCLFAVGCKGPAANCPWNKVGWVNGVGG
CHHHCCCHHHHCCCCCCCCCCCHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCC
PTRTGGVCIGCTMPGFSDAFEPFYKPLPAPAAPSAPEALATAVGAAAIGAVVGYALTKKA
CCCCCCEEEEECCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
KG
CC
>Mature Secondary Structure 
SVTKPRREFLKKLGASTLLLGALSNIDWANAMEMFKEAVRKGKVNIVWFEAQSCTGDTT
CCCHHHHHHHHHHCCHHEEHHHHCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCH
SLIQATDPDLVEVLTGAIHLAGPGTVGLAFHDTVMPEWGEGAIEILKLAESGAYDPFVLV
HHEECCCCHHHHHHHHHEEECCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCEEEE
IEGSIPDENHARKFCKDPNRCGYYCFVGEENGRVVTCTEWIHRLAKRAVAVVAAGTCASY
EECCCCCHHHHHHHHCCCCCCEEEEEEECCCCCEEEHHHHHHHHHHHHHHHHCCCHHHHC
GGIPSNKVLEPPPGWSYPEWSLAPTGAVGFFDDPVKGYKGLISRHAELPNLTPYFNYIFR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHH
DCEPELSPGSTCKPAVAVPGCPTNGNGILRVLVHLVLAALYPDVVPLPRASEWLDQYARP
CCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCC
KYLFGRTVHEQCPRAGYYAAGDLRRLPGDNDAKCLFAVGCKGPAANCPWNKVGWVNGVGG
CHHHCCCHHHHCCCCCCCCCCCHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCC
PTRTGGVCIGCTMPGFSDAFEPFYKPLPAPAAPSAPEALATAVGAAAIGAVVGYALTKKA
CCCCCCEEEEECCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
KG
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3316183; 3322275; 10378275 [H]