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The map label for this gene is prf1 [H]

Identifier: 156938131

GI number: 156938131

Start: 1195572

End: 1196663

Strand: Reverse

Name: prf1 [H]

Synonym: Igni_1344

Alternate gene names: 156938131

Gene position: 1196663-1195572 (Counterclockwise)

Preceding gene: 156938132

Following gene: 156938130

Centisome position: 92.23

GC content: 56.96

Gene sequence:

>1092_bases
GTGTCTCAGACCAAGGGCGTGGAGATCAGTAGGGCCGAGCTCAAGCGGCTCTTGAAGGAATTGGAGCAGTGGAGCGCCCC
CGCGACGACCCTCTTGAGCTTGTACATACCGCCCGGCAGGCCGGTAGGGGACGTAATGGAGCTCTTGAGGAAAGAGCTCA
GCGTCGCTGACAACGTCAAGTTGAAGAAGACCAGGGAAGCGGTCAAGGCGGCTCTGACGGCAGCAATGGAGAGGTTAAAG
ATGTTCGACAAGGTTCCTCCTAACGGGCTAGTGATATTCTGCGGCGTAAACCCGAACACGGAGAAGCTGGAGTGCTTCAC
CTTCTCGCCGCCGGACAAGGTTCCGGTCTTCTTCTACAGGACGGACAAGAAGTTCCACACGGAGTTCCTCAAGGATATGG
TCGAGGAACCCGAAGTTTACGGCTTAATACTGGTGGAGAGGGGAAGGATGATCGTGGGGGCACTCAAGGGCTCCAGGATA
GAGGTCCTCGGAGAGGCCACAGGCTTCATACCCGGTAAACACCACCGAGGCGGCCAGTCCCAGAGGAGGTTCGACCGACT
GATAGAACAAGCGGCCGAGGCGTTCTACAAACACGCCGGTGAGAGGGCCTCTCAGCTGCTGCTGCCTTATCTAGAACAAA
AGAGGTTGAAGGGCGTCCTCATAGGTGGTCCCGCCTTCTCGAAGCAAGACTTCTTAGAGGCCGGGGGGCTGGACTACCGA
ATAAAACAGCTCACCATAGGCCTTTACGACGTAGCGGATGTGGACGAACACGGGCTTCACGAGCTCGTCCAAAGGGCGGC
GGATGACATAAAGGGCCAGAAGTACGCCGAGGCAACTAAGGCCTTGGAGGAGTTCAAGCGACACTTGGCGAGGGACACGG
GCTACGCAGTATACGGCGAGGCCGAGACCCTGGAGGCGTTGAACATGGGCATGTTGAAAACTTTATTGATATCCGAGGAT
CACCCGAGGCTGGAGGAGATAACGGAGCTCGCGAAGAGGTACGGTACAGAAGTGGTCGTAATAGAGGGCGTCCCGGAGGC
CGAGTGGTTCAAGAAGACGTTCGGCATAGCCGGGATAAAGAGGTTCAAGTGA

Upstream 100 bases:

>100_bases
ACGGAAAAGGGTTCCTTGGTCTTTCAGAACTTCGTCGAGCTGTGCAAGAGGTGACGTGTATAAATACAGTTTTTTACCCC
CGATAGGGCGGGGCGCGAAA

Downstream 100 bases:

>100_bases
GTTTTCGGGGGCAGTCGTGGAGCCGCTAACCCTCTTCACGCTCTTCCACGTGTTCGTAATTGATGGAGACACTTTCGTTT
ATCACGGCGTGAAGTTCAGG

Product: peptide chain release factor 1

Products: NA

Alternate protein names: Translation termination factor aRF1 [H]

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MSQTKGVEISRAELKRLLKELEQWSAPATTLLSLYIPPGRPVGDVMELLRKELSVADNVKLKKTREAVKAALTAAMERLK
MFDKVPPNGLVIFCGVNPNTEKLECFTFSPPDKVPVFFYRTDKKFHTEFLKDMVEEPEVYGLILVERGRMIVGALKGSRI
EVLGEATGFIPGKHHRGGQSQRRFDRLIEQAAEAFYKHAGERASQLLLPYLEQKRLKGVLIGGPAFSKQDFLEAGGLDYR
IKQLTIGLYDVADVDEHGLHELVQRAADDIKGQKYAEATKALEEFKRHLARDTGYAVYGEAETLEALNMGMLKTLLISED
HPRLEEITELAKRYGTEVVVIEGVPEAEWFKKTFGIAGIKRFK

Sequences:

>Translated_363_residues
MSQTKGVEISRAELKRLLKELEQWSAPATTLLSLYIPPGRPVGDVMELLRKELSVADNVKLKKTREAVKAALTAAMERLK
MFDKVPPNGLVIFCGVNPNTEKLECFTFSPPDKVPVFFYRTDKKFHTEFLKDMVEEPEVYGLILVERGRMIVGALKGSRI
EVLGEATGFIPGKHHRGGQSQRRFDRLIEQAAEAFYKHAGERASQLLLPYLEQKRLKGVLIGGPAFSKQDFLEAGGLDYR
IKQLTIGLYDVADVDEHGLHELVQRAADDIKGQKYAEATKALEEFKRHLARDTGYAVYGEAETLEALNMGMLKTLLISED
HPRLEEITELAKRYGTEVVVIEGVPEAEWFKKTFGIAGIKRFK
>Mature_362_residues
SQTKGVEISRAELKRLLKELEQWSAPATTLLSLYIPPGRPVGDVMELLRKELSVADNVKLKKTREAVKAALTAAMERLKM
FDKVPPNGLVIFCGVNPNTEKLECFTFSPPDKVPVFFYRTDKKFHTEFLKDMVEEPEVYGLILVERGRMIVGALKGSRIE
VLGEATGFIPGKHHRGGQSQRRFDRLIEQAAEAFYKHAGERASQLLLPYLEQKRLKGVLIGGPAFSKQDFLEAGGLDYRI
KQLTIGLYDVADVDEHGLHELVQRAADDIKGQKYAEATKALEEFKRHLARDTGYAVYGEAETLEALNMGMLKTLLISEDH
PRLEEITELAKRYGTEVVVIEGVPEAEWFKKTFGIAGIKRFK

Specific function: Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA [H]

COG id: COG1503

COG function: function code J; Peptide chain release factor 1 (eRF1)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eukaryotic release factor 1 family [H]

Homologues:

Organism=Homo sapiens, GI4759034, Length=376, Percent_Identity=29.2553191489362, Blast_Score=175, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI72000368, Length=373, Percent_Identity=30.2949061662198, Blast_Score=177, Evalue=7e-45,
Organism=Saccharomyces cerevisiae, GI6319619, Length=355, Percent_Identity=30.4225352112676, Blast_Score=168, Evalue=1e-42,
Organism=Drosophila melanogaster, GI28574632, Length=384, Percent_Identity=30.2083333333333, Blast_Score=181, Evalue=8e-46,
Organism=Drosophila melanogaster, GI21356433, Length=384, Percent_Identity=30.2083333333333, Blast_Score=181, Evalue=8e-46,
Organism=Drosophila melanogaster, GI24667392, Length=384, Percent_Identity=30.2083333333333, Blast_Score=181, Evalue=8e-46,
Organism=Drosophila melanogaster, GI24667388, Length=384, Percent_Identity=30.2083333333333, Blast_Score=181, Evalue=8e-46,
Organism=Drosophila melanogaster, GI24667380, Length=384, Percent_Identity=30.2083333333333, Blast_Score=181, Evalue=8e-46,
Organism=Drosophila melanogaster, GI24667384, Length=384, Percent_Identity=30.2083333333333, Blast_Score=181, Evalue=8e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005140
- InterPro:   IPR005141
- InterPro:   IPR005142
- InterPro:   IPR020918
- InterPro:   IPR004403 [H]

Pfam domain/function: PF03463 eRF1_1; PF03464 eRF1_2; PF03465 eRF1_3 [H]

EC number: NA

Molecular weight: Translated: 40864; Mature: 40733

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTKGVEISRAELKRLLKELEQWSAPATTLLSLYIPPGRPVGDVMELLRKELSVADNVK
CCCCCCCEECHHHHHHHHHHHHHCCCCHHHEEEEECCCCCCHHHHHHHHHHHHHHHCCCH
LKKTREAVKAALTAAMERLKMFDKVPPNGLVIFCGVNPNTEKLECFTFSPPDKVPVFFYR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEECCCCCCCEEEEEE
TDKKFHTEFLKDMVEEPEVYGLILVERGRMIVGALKGSRIEVLGEATGFIPGKHHRGGQS
CCHHHHHHHHHHHHCCCCEEEEEEEECCCEEEEECCCCEEEEEECCCCCCCCCCCCCCCH
QRRFDRLIEQAAEAFYKHAGERASQLLLPYLEQKRLKGVLIGGPAFSKQDFLEAGGLDYR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCHHHHHHHCCCCEE
IKQLTIGLYDVADVDEHGLHELVQRAADDIKGQKYAEATKALEEFKRHLARDTGYAVYGE
EEEEEEEHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEECC
AETLEALNMGMLKTLLISEDHPRLEEITELAKRYGTEVVVIEGVPEAEWFKKTFGIAGIK
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHCCHH
RFK
CCC
>Mature Secondary Structure 
SQTKGVEISRAELKRLLKELEQWSAPATTLLSLYIPPGRPVGDVMELLRKELSVADNVK
CCCCCCEECHHHHHHHHHHHHHCCCCHHHEEEEECCCCCCHHHHHHHHHHHHHHHCCCH
LKKTREAVKAALTAAMERLKMFDKVPPNGLVIFCGVNPNTEKLECFTFSPPDKVPVFFYR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEECCCCCCCEEEEEE
TDKKFHTEFLKDMVEEPEVYGLILVERGRMIVGALKGSRIEVLGEATGFIPGKHHRGGQS
CCHHHHHHHHHHHHCCCCEEEEEEEECCCEEEEECCCCEEEEEECCCCCCCCCCCCCCCH
QRRFDRLIEQAAEAFYKHAGERASQLLLPYLEQKRLKGVLIGGPAFSKQDFLEAGGLDYR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCHHHHHHHCCCCEE
IKQLTIGLYDVADVDEHGLHELVQRAADDIKGQKYAEATKALEEFKRHLARDTGYAVYGE
EEEEEEEHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEECC
AETLEALNMGMLKTLLISEDHPRLEEITELAKRYGTEVVVIEGVPEAEWFKKTFGIAGIK
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHCCHH
RFK
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]