Click here to switch to the map view.

The map label for this gene is rfcL

Identifier: 156938087

GI number: 156938087

Start: 1155538

End: 1156968

Strand: Direct

Name: rfcL

Synonym: Igni_1300

Alternate gene names: 156938087

Gene position: 1155538-1156968 (Clockwise)

Preceding gene: 156938086

Following gene: 156938089

Centisome position: 89.06

GC content: 52.9

Gene sequence:

>1431_bases
TTGAGCGCAGTACCGTGGATAATAAAGTATAGGCCAAAAAGGGTAGAGGACGTTATAGACCAAGAAAAGGCTAAGGAGGT
GTTAATACCTTGGATAAAGAAGTGGCTGAGCGGCACACCTCCGGAGAAGAGGGCCGCCCTGTTGTGGGGTCCTCCGGGCG
TGGGAAAGACTAGCTTGGTCGAGGCTATATGCAACGAATTCAACTTAGAAAAAATTGAGATGAACGCTAGCGACTTCAGG
AGGAAAGGAGATATAGAGCGCGTAGCCATAGCCGCCGCCACCAAGAAGCCCTTGCCGCCTTGGAAGGGGCGCTTGATCTT
GTTAGACGAGGTGGACGGGCTCTCGCCCAGGGGGGACGAGGGGGCCGTCGCGGCTATACTGGAATTAATAAAGAAGACGA
AGAACCCTATCGTAATGACGGCCAACGACCCGTGGGGGACTCACCTAAGGCCGATAAGGGAGGAGAGCTTGTTGGTCGAG
TTCAAAAGGATACCCAAGACCAAGGCCCGCGAGTTCTTACTAAAAATATGCGAAAAGGAGGGCGTTTATTGCGAGAAGGA
GGCTGTGGACTACATCATAGAAAAGAACAAGGGAGACTTGAGGGCGTCGATCAACGACTTACAATCCATCGCTGAAGCCT
ACGGCAAGGTAACCTTAGACTTGGCGAGCGCCTTGTTGGTCGAGAGGGACAGGGTGCTAACTCCTTGGGAGATGCTGCAG
AGCTTGTTCTACTCCAAGTACTCTTGGCAAGCTAGGAAGGCCGTAACGAGTACCGACTTGGACTACGACACCCTATTCTT
GTGGATAGCTGAAAACGTCCCCAAGCAGTACGGCGACGACCCTTACGACTTATGGAGGGGAATGGAAGCAGTTTCGAGGG
CGGACGTGATATACGGCAGGATAAGGAGGACTATGAACTGGTCCTTGTTACCCTACTTCTTCAACGCCTTGGGCCCCGGG
GTGGCGCTCGCCAAGACCAAGTACCACAAGAGGGCAAGGTGGAGCTACCCGGAGAAGATAGTGCTGTTGGCGAGGACTAA
GGAAGAGAGGAGGGTCAGGGACGAGCTCGCCTCTCACTTGGCGCTCTTGACGCACTCCTCCAAATCCTACGTGAGGAGGG
AAATAATACCTATGTTGCAGTTCATCGCCCAAGTGAACCCGAACTACTTCGCGCGGCTGGCGCTGGGCCTCCGCTTGAGC
GACCCTATGATAAAGTACTTAGCAAAGAGTAAGTATCAATTAGTAAAGAAGTACGTAGAAGAGTTGAAGAGTTCCAGAAC
GGTAAAGGCGGAAGAACAAACCAAAGCGAAGGAGGTGGTAAAGGAACTCAAGAAAGAGTACGAGAAGGGCACGAAGAAGG
GGAAGGGCGAGAAGAGGCGGAAGGGCTCGGACGAGGGCTCGGGCTTGCTGAAGTGGCTCAAGAAAGATTGA

Upstream 100 bases:

>100_bases
AGTTCAGGCTGGCAGAGGGCGCGGACGACGAGGTCCAGCTACAAGCGTTCTTGGCGAGGCTGGCCCTGTTGGGCCCTAAG
TTCAAGGGAGGTGCTAAGCG

Downstream 100 bases:

>100_bases
ACTGTTCCAAGAGCCTCTTGGTCTTCTCCTTTACTTTGTCCACCTCTCCCCAGTCCACGGGGTGTAAGAAGAACTTGTCA
GCCTTGTCCGCTAACTTCAA

Product: replication factor C large subunit

Products: NA

Alternate protein names: RFC large subunit; Clamp loader large subunit

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MSAVPWIIKYRPKRVEDVIDQEKAKEVLIPWIKKWLSGTPPEKRAALLWGPPGVGKTSLVEAICNEFNLEKIEMNASDFR
RKGDIERVAIAAATKKPLPPWKGRLILLDEVDGLSPRGDEGAVAAILELIKKTKNPIVMTANDPWGTHLRPIREESLLVE
FKRIPKTKAREFLLKICEKEGVYCEKEAVDYIIEKNKGDLRASINDLQSIAEAYGKVTLDLASALLVERDRVLTPWEMLQ
SLFYSKYSWQARKAVTSTDLDYDTLFLWIAENVPKQYGDDPYDLWRGMEAVSRADVIYGRIRRTMNWSLLPYFFNALGPG
VALAKTKYHKRARWSYPEKIVLLARTKEERRVRDELASHLALLTHSSKSYVRREIIPMLQFIAQVNPNYFARLALGLRLS
DPMIKYLAKSKYQLVKKYVEELKSSRTVKAEEQTKAKEVVKELKKEYEKGTKKGKGEKRRKGSDEGSGLLKWLKKD

Sequences:

>Translated_476_residues
MSAVPWIIKYRPKRVEDVIDQEKAKEVLIPWIKKWLSGTPPEKRAALLWGPPGVGKTSLVEAICNEFNLEKIEMNASDFR
RKGDIERVAIAAATKKPLPPWKGRLILLDEVDGLSPRGDEGAVAAILELIKKTKNPIVMTANDPWGTHLRPIREESLLVE
FKRIPKTKAREFLLKICEKEGVYCEKEAVDYIIEKNKGDLRASINDLQSIAEAYGKVTLDLASALLVERDRVLTPWEMLQ
SLFYSKYSWQARKAVTSTDLDYDTLFLWIAENVPKQYGDDPYDLWRGMEAVSRADVIYGRIRRTMNWSLLPYFFNALGPG
VALAKTKYHKRARWSYPEKIVLLARTKEERRVRDELASHLALLTHSSKSYVRREIIPMLQFIAQVNPNYFARLALGLRLS
DPMIKYLAKSKYQLVKKYVEELKSSRTVKAEEQTKAKEVVKELKKEYEKGTKKGKGEKRRKGSDEGSGLLKWLKKD
>Mature_475_residues
SAVPWIIKYRPKRVEDVIDQEKAKEVLIPWIKKWLSGTPPEKRAALLWGPPGVGKTSLVEAICNEFNLEKIEMNASDFRR
KGDIERVAIAAATKKPLPPWKGRLILLDEVDGLSPRGDEGAVAAILELIKKTKNPIVMTANDPWGTHLRPIREESLLVEF
KRIPKTKAREFLLKICEKEGVYCEKEAVDYIIEKNKGDLRASINDLQSIAEAYGKVTLDLASALLVERDRVLTPWEMLQS
LFYSKYSWQARKAVTSTDLDYDTLFLWIAENVPKQYGDDPYDLWRGMEAVSRADVIYGRIRRTMNWSLLPYFFNALGPGV
ALAKTKYHKRARWSYPEKIVLLARTKEERRVRDELASHLALLTHSSKSYVRREIIPMLQFIAQVNPNYFARLALGLRLSD
PMIKYLAKSKYQLVKKYVEELKSSRTVKAEEQTKAKEVVKELKKEYEKGTKKGKGEKRRKGSDEGSGLLKWLKKD

Specific function: Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the activator 1 small subunits family. RfcL subfamily

Homologues:

Organism=Homo sapiens, GI32528306, Length=420, Percent_Identity=24.7619047619048, Blast_Score=132, Evalue=8e-31,
Organism=Homo sapiens, GI4506491, Length=247, Percent_Identity=30.3643724696356, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI31881687, Length=247, Percent_Identity=30.3643724696356, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI31563534, Length=223, Percent_Identity=25.5605381165919, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI6677723, Length=225, Percent_Identity=27.1111111111111, Blast_Score=75, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17563226, Length=422, Percent_Identity=25.3554502369668, Blast_Score=123, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17541988, Length=217, Percent_Identity=28.5714285714286, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17554730, Length=221, Percent_Identity=28.9592760180996, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17541368, Length=441, Percent_Identity=21.9954648526077, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71986063, Length=232, Percent_Identity=25.4310344827586, Blast_Score=65, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6324791, Length=420, Percent_Identity=23.3333333333333, Blast_Score=106, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6324478, Length=224, Percent_Identity=27.6785714285714, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6322528, Length=233, Percent_Identity=29.6137339055794, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323724, Length=253, Percent_Identity=25.296442687747, Blast_Score=77, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6324039, Length=228, Percent_Identity=29.8245614035088, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI17737765, Length=419, Percent_Identity=25.7756563245823, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI62472391, Length=358, Percent_Identity=27.3743016759777, Blast_Score=119, Evalue=6e-27,
Organism=Drosophila melanogaster, GI18859927, Length=221, Percent_Identity=31.6742081447964, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI28574716, Length=324, Percent_Identity=24.3827160493827, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI17647857, Length=224, Percent_Identity=25, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19921076, Length=225, Percent_Identity=27.5555555555556, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RFCL_IGNH4 (A8AC24)

Other databases:

- EMBL:   CP000816
- RefSeq:   YP_001435883.1
- ProteinModelPortal:   A8AC24
- SMR:   A8AC24
- STRING:   A8AC24
- GeneID:   5562218
- GenomeReviews:   CP000816_GR
- KEGG:   iho:Igni_1300
- eggNOG:   arNOG04036
- HOGENOM:   HBG541322
- OMA:   ELNASDK
- ProtClustDB:   PRK04195
- BioCyc:   IHOS453591:IGNI_1300-MONOMER
- HAMAP:   MF_01508
- InterPro:   IPR003593
- InterPro:   IPR003959
- InterPro:   IPR008921
- InterPro:   IPR013725
- SMART:   SM00382

Pfam domain/function: PF00004 AAA; PF08519 RFC1; SSF48019 Pol_clamp_load_C

EC number: NA

Molecular weight: Translated: 54711; Mature: 54580

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAVPWIIKYRPKRVEDVIDQEKAKEVLIPWIKKWLSGTPPEKRAALLWGPPGVGKTSLV
CCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHH
EAICNEFNLEKIEMNASDFRRKGDIERVAIAAATKKPLPPWKGRLILLDEVDGLSPRGDE
HHHHHHCCCCEEECCHHHHHHCCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCC
GAVAAILELIKKTKNPIVMTANDPWGTHLRPIREESLLVEFKRIPKTKAREFLLKICEKE
HHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
GVYCEKEAVDYIIEKNKGDLRASINDLQSIAEAYGKVTLDLASALLVERDRVLTPWEMLQ
CCEECHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHH
SLFYSKYSWQARKAVTSTDLDYDTLFLWIAENVPKQYGDDPYDLWRGMEAVSRADVIYGR
HHHHHHHCHHHHHHHHCCCCCHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHH
IRRTMNWSLLPYFFNALGPGVALAKTKYHKRARWSYPEKIVLLARTKEERRVRDELASHL
HHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHH
ALLTHSSKSYVRREIIPMLQFIAQVNPNYFARLALGLRLSDPMIKYLAKSKYQLVKKYVE
HHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
ELKSSRTVKAEEQTKAKEVVKELKKEYEKGTKKGKGEKRRKGSDEGSGLLKWLKKD
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
SAVPWIIKYRPKRVEDVIDQEKAKEVLIPWIKKWLSGTPPEKRAALLWGPPGVGKTSLV
CCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHH
EAICNEFNLEKIEMNASDFRRKGDIERVAIAAATKKPLPPWKGRLILLDEVDGLSPRGDE
HHHHHHCCCCEEECCHHHHHHCCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCC
GAVAAILELIKKTKNPIVMTANDPWGTHLRPIREESLLVEFKRIPKTKAREFLLKICEKE
HHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
GVYCEKEAVDYIIEKNKGDLRASINDLQSIAEAYGKVTLDLASALLVERDRVLTPWEMLQ
CCEECHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHH
SLFYSKYSWQARKAVTSTDLDYDTLFLWIAENVPKQYGDDPYDLWRGMEAVSRADVIYGR
HHHHHHHCHHHHHHHHCCCCCHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHH
IRRTMNWSLLPYFFNALGPGVALAKTKYHKRARWSYPEKIVLLARTKEERRVRDELASHL
HHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHH
ALLTHSSKSYVRREIIPMLQFIAQVNPNYFARLALGLRLSDPMIKYLAKSKYQLVKKYVE
HHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
ELKSSRTVKAEEQTKAKEVVKELKKEYEKGTKKGKGEKRRKGSDEGSGLLKWLKKD
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA