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The map label for this gene is rfc [H]

Identifier: 156938086

GI number: 156938086

Start: 1154552

End: 1155541

Strand: Direct

Name: rfc [H]

Synonym: Igni_1299

Alternate gene names: 156938086

Gene position: 1154552-1155541 (Clockwise)

Preceding gene: 156938083

Following gene: 156938087

Centisome position: 88.98

GC content: 54.55

Gene sequence:

>990_bases
TTGTCCGCTCCGGAAGAGTTGTTGTGGGCAGAGAAGTACAGGCCGAAGAGCTTAGACGAAATAGTGGATCAAGAGGACAT
AGTGAGGAGACTGAAGAAGTTCGTAGAGGAGAAGAACGTGCCGCACATGCTCTTCGCCGGCCCTCCGGGAACGGGGAAGA
CCACCGCGGCCCTCGCCCTCGCCCACGACCTTTATGGAGAAAAGTATAGGCAATACATCTTGGAACTTAACGCAAGCGAC
GAAAGAGGCATAGACGTTATAAGGACCAAGGTGAAAGAGTTCGCGAGGTCTAGGACCCCTCCCACAGTCCCCTTCAAGTT
GGTCATACTGGACGAGGCCGACAACATGACCGCCGACGCCCAGCAAGCCCTCAGGAGGCTTATGGAGATGTACTCCACTA
CCACTAGGTTCATACTGTTAGCTAACTTCCCGAGCAAGATAATTGAGCCCGTGCAGTCTAGGTGCGTGTACTTCCGCTTC
CGCCCCCTGCCTAAGGATAAGGTAATAGAAAGACTCAAGTACATCTGTCAGAAGGAAGGGGTCCAATGCGAGGAGGACGC
TCTGGAGGAGATATACAACATCTCGGAAGGAGACATGAGGAAGGCTATAAACATCCTTCAAGCAGCAGCTGCCTTGGGCA
AGGTAACTAAAGACGCGGTCTATAAGGCCATAGGCTACGTCCATCCGAGCAAGATAAAGGAGATCCTCGAGTATGCCCTC
AACGGCGACTTCACCAAGTCCGCTAAGCTTCTAAGGGACGTCATGATAGAGTACGGCCTCTCCGGGCTGGACGTGCTGAA
GATGTTCCAAAGGGAGTTGATGGGCGGGAGCTTCGAGCTTCCGGAAGAGCTCAAAGTGTTGCTGGCGGACTACGCGGGAG
AGGTGCAGTTCAGGCTGGCAGAGGGCGCGGACGACGAGGTCCAGCTACAAGCGTTCTTGGCGAGGCTGGCCCTGTTGGGC
CCTAAGTTCAAGGGAGGTGCTAAGCGTTGA

Upstream 100 bases:

>100_bases
CCGGCGCGCCTCTAATCAAGCGGCTGAGGTGTAGCACAACTTTCAGCCCTTTGACCAGTTATACTCCCGGCGCCGTCGAG
AGGCTCGGGGAAAAGGCTCC

Downstream 100 bases:

>100_bases
GCGCAGTACCGTGGATAATAAAGTATAGGCCAAAAAGGGTAGAGGACGTTATAGACCAAGAAAAGGCTAAGGAGGTGTTA
ATACCTTGGATAAAGAAGTG

Product: replication factor C small subunit

Products: N Pyrophosphate; DNA(N). [C]

Alternate protein names: RFC small subunit; Clamp loader small subunit [H]

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MSAPEELLWAEKYRPKSLDEIVDQEDIVRRLKKFVEEKNVPHMLFAGPPGTGKTTAALALAHDLYGEKYRQYILELNASD
ERGIDVIRTKVKEFARSRTPPTVPFKLVILDEADNMTADAQQALRRLMEMYSTTTRFILLANFPSKIIEPVQSRCVYFRF
RPLPKDKVIERLKYICQKEGVQCEEDALEEIYNISEGDMRKAINILQAAAALGKVTKDAVYKAIGYVHPSKIKEILEYAL
NGDFTKSAKLLRDVMIEYGLSGLDVLKMFQRELMGGSFELPEELKVLLADYAGEVQFRLAEGADDEVQLQAFLARLALLG
PKFKGGAKR

Sequences:

>Translated_329_residues
MSAPEELLWAEKYRPKSLDEIVDQEDIVRRLKKFVEEKNVPHMLFAGPPGTGKTTAALALAHDLYGEKYRQYILELNASD
ERGIDVIRTKVKEFARSRTPPTVPFKLVILDEADNMTADAQQALRRLMEMYSTTTRFILLANFPSKIIEPVQSRCVYFRF
RPLPKDKVIERLKYICQKEGVQCEEDALEEIYNISEGDMRKAINILQAAAALGKVTKDAVYKAIGYVHPSKIKEILEYAL
NGDFTKSAKLLRDVMIEYGLSGLDVLKMFQRELMGGSFELPEELKVLLADYAGEVQFRLAEGADDEVQLQAFLARLALLG
PKFKGGAKR
>Mature_328_residues
SAPEELLWAEKYRPKSLDEIVDQEDIVRRLKKFVEEKNVPHMLFAGPPGTGKTTAALALAHDLYGEKYRQYILELNASDE
RGIDVIRTKVKEFARSRTPPTVPFKLVILDEADNMTADAQQALRRLMEMYSTTTRFILLANFPSKIIEPVQSRCVYFRFR
PLPKDKVIERLKYICQKEGVQCEEDALEEIYNISEGDMRKAINILQAAAALGKVTKDAVYKAIGYVHPSKIKEILEYALN
GDFTKSAKLLRDVMIEYGLSGLDVLKMFQRELMGGSFELPEELKVLLADYAGEVQFRLAEGADDEVQLQAFLARLALLGP
KFKGGAKR

Specific function: Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the activator 1 small subunits family. RfcS subfamily [H]

Homologues:

Organism=Homo sapiens, GI6677723, Length=311, Percent_Identity=42.1221864951768, Blast_Score=265, Evalue=4e-71,
Organism=Homo sapiens, GI194306571, Length=307, Percent_Identity=42.0195439739414, Blast_Score=260, Evalue=1e-69,
Organism=Homo sapiens, GI194306567, Length=307, Percent_Identity=42.0195439739414, Blast_Score=260, Evalue=1e-69,
Organism=Homo sapiens, GI31563534, Length=314, Percent_Identity=43.312101910828, Blast_Score=252, Evalue=4e-67,
Organism=Homo sapiens, GI4506491, Length=319, Percent_Identity=42.9467084639498, Blast_Score=240, Evalue=2e-63,
Organism=Homo sapiens, GI31881687, Length=319, Percent_Identity=42.9467084639498, Blast_Score=240, Evalue=2e-63,
Organism=Homo sapiens, GI28882049, Length=313, Percent_Identity=37.6996805111821, Blast_Score=203, Evalue=2e-52,
Organism=Homo sapiens, GI194306569, Length=243, Percent_Identity=41.5637860082305, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI4506489, Length=232, Percent_Identity=34.4827586206897, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI108773789, Length=232, Percent_Identity=34.4827586206897, Blast_Score=122, Evalue=6e-28,
Organism=Escherichia coli, GI1786676, Length=294, Percent_Identity=24.4897959183673, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71986063, Length=319, Percent_Identity=41.0658307210031, Blast_Score=251, Evalue=4e-67,
Organism=Caenorhabditis elegans, GI17541988, Length=313, Percent_Identity=41.5335463258786, Blast_Score=234, Evalue=3e-62,
Organism=Caenorhabditis elegans, GI17554730, Length=323, Percent_Identity=37.7708978328173, Blast_Score=216, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI17541990, Length=237, Percent_Identity=34.1772151898734, Blast_Score=116, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6324039, Length=319, Percent_Identity=38.5579937304075, Blast_Score=247, Evalue=2e-66,
Organism=Saccharomyces cerevisiae, GI6322528, Length=330, Percent_Identity=41.5151515151515, Blast_Score=244, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6324478, Length=319, Percent_Identity=41.3793103448276, Blast_Score=242, Evalue=6e-65,
Organism=Saccharomyces cerevisiae, GI6319562, Length=240, Percent_Identity=33.75, Blast_Score=118, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6324791, Length=236, Percent_Identity=26.6949152542373, Blast_Score=72, Evalue=9e-14,
Organism=Drosophila melanogaster, GI18859927, Length=314, Percent_Identity=41.7197452229299, Blast_Score=249, Evalue=1e-66,
Organism=Drosophila melanogaster, GI19921076, Length=309, Percent_Identity=40.7766990291262, Blast_Score=241, Evalue=6e-64,
Organism=Drosophila melanogaster, GI17647857, Length=319, Percent_Identity=41.692789968652, Blast_Score=239, Evalue=2e-63,
Organism=Drosophila melanogaster, GI19921136, Length=348, Percent_Identity=26.1494252873563, Blast_Score=119, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003959
- InterPro:   IPR000641
- InterPro:   IPR008921
- InterPro:   IPR013748 [H]

Pfam domain/function: PF00004 AAA; PF08542 Rep_fac_C [H]

EC number: 2.7.7.7 [C]

Molecular weight: Translated: 37302; Mature: 37171

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00039 DEAD_ATP_HELICASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPEELLWAEKYRPKSLDEIVDQEDIVRRLKKFVEEKNVPHMLFAGPPGTGKTTAALAL
CCCCHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHH
AHDLYGEKYRQYILELNASDERGIDVIRTKVKEFARSRTPPTVPFKLVILDEADNMTADA
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHH
QQALRRLMEMYSTTTRFILLANFPSKIIEPVQSRCVYFRFRPLPKDKVIERLKYICQKEG
HHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHCC
VQCEEDALEEIYNISEGDMRKAINILQAAAALGKVTKDAVYKAIGYVHPSKIKEILEYAL
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
NGDFTKSAKLLRDVMIEYGLSGLDVLKMFQRELMGGSFELPEELKVLLADYAGEVQFRLA
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEEC
EGADDEVQLQAFLARLALLGPKFKGGAKR
CCCCHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SAPEELLWAEKYRPKSLDEIVDQEDIVRRLKKFVEEKNVPHMLFAGPPGTGKTTAALAL
CCCHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHH
AHDLYGEKYRQYILELNASDERGIDVIRTKVKEFARSRTPPTVPFKLVILDEADNMTADA
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHH
QQALRRLMEMYSTTTRFILLANFPSKIIEPVQSRCVYFRFRPLPKDKVIERLKYICQKEG
HHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHCC
VQCEEDALEEIYNISEGDMRKAINILQAAAALGKVTKDAVYKAIGYVHPSKIKEILEYAL
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
NGDFTKSAKLLRDVMIEYGLSGLDVLKMFQRELMGGSFELPEELKVLLADYAGEVQFRLA
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEEC
EGADDEVQLQAFLARLALLGPKFKGGAKR
CCCCHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Dimethyl sulfoxide epsilon,; Polymerase alpha accessory factors; Proliferating cell nuclear antigen; Replication factor A; Replication factor C; Thiol [C]

Metal ions: K+; Mg2+; Mn2+; Na+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0063 {dATP}} [C]

Substrates: N Deoxynucleoside Triphosphate; DNA [C]

Specific reaction: N Deoxynucleoside Triphosphate = N Pyrophosphate + DNA(N). Protein + DNA = Protein-DNA [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; 2', 3'-Dideoxythymidine5'-triphosphate polymerase beta, gamma, delta, epsilon; Aphidicolin polymerase alpha; Ara-ATP; Ara-CTP; Benzyl oxycarbonyl -Leu-Leu-al; Carbonyl diphosphonate delta, alpha,; Dideoxynucleoside 5'-triphosphate;

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA