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The map label for this gene is rfbB [C]

Identifier: 156937783

GI number: 156937783

Start: 884637

End: 885518

Strand: Reverse

Name: rfbB [C]

Synonym: Igni_0993

Alternate gene names: 156937783

Gene position: 885518-884637 (Counterclockwise)

Preceding gene: 156937784

Following gene: 156937781

Centisome position: 68.25

GC content: 59.3

Gene sequence:

>882_bases
TTGATCTGCATAACGGGGGGCTCCGGCTACATAGGTTCTAAGCTGGTCGAAGAACTCTTGAAAGAAGGCGAGGTAAAGGT
ACTGGACCTCGCTCCCCCTCCGGTCCCTCACGTGAAGTTCACGAGGGTGAACGTGCTCTTGTTGGACGACTTGAAGGTCG
AGCTGAGGGACTGCGAGCTGGTCTACCACCTAGCGGCCGAGATCAAGGCGGAGGAGTCCTTGAGGGAGCCCGCAAAGGTC
GTGAGGGTGAACGTGGAAGGTACCTTGAACGTGTTGGAGGCCGCGAGGCTCGCGGACGCTTCTGTGGTGTTCGCCTCTAC
GGCCGCAGTCTACGGGGAAGCCAAGGTCGTCCCGGTGCCGGAGGAACACCCGTTGGAACCGGTAAACGTCTACGGCGCCA
CTAAGGTCGCGGGCGAGGCCTTGGTCAATTCCTACCGCAAGGCCTTCGGGTTGAGGGCTTGGACCTTGAGGCTGTTCAAC
GTTTACGGCCCCAGCGCCTCCCCCAGTCGGGGGGTGGTGGGAGAGTTCTTGAGGAGGGCGCTGAAGGGGGAGCCCTTGAG
GATTTACGGCGACGGGAGGCAAGTTAGGGACTTCGTGTTTGTAGACGACGTCGTGAAAGCGTTCAAGTTGGTAAGGGAAA
TCCCCGAAGGAACTTACAACGTCGGGAGCGGCCGCGGCGTGAGCATAATAACCCTAGCAAAGAAAATTATTGAACTAACT
GGCTCGAAGTCAGAGATGGTCTTCCTCCCCGAGAGGCCTGGCGACGTGAGGGTTAGCGTTGCAGACGTCACGAAGCTGGC
GGCCTTCGGGTGGAGGCCTAGGGTTAGCTTGGAAGAGGGTTTGAGGCTGACCGCGGAGGCCCTTAAGGCAAGGGGAACTT
GA

Upstream 100 bases:

>100_bases
GAAGGAGACCCTCAAGATGTACGAAGAGTGGTTCAAGGCCCACGGCGCGCTCTGAAGGCTTTTCTGTCCTCCTCGCCTCT
CGGCTCCCGGAAAGGTCGTC

Downstream 100 bases:

>100_bases
CCGCCGCGTACCCTACTACCGCGCTCTTGTCGCCGGAAGTGTCCCCGGACGTGGCGTGCTTGAGCAAGATCGCCCTCTCC
GCGCCGTACTCCTTGTCCAA

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: Galactowaldenase; UDP-galactose 4-epimerase [H]

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MICITGGSGYIGSKLVEELLKEGEVKVLDLAPPPVPHVKFTRVNVLLLDDLKVELRDCELVYHLAAEIKAEESLREPAKV
VRVNVEGTLNVLEAARLADASVVFASTAAVYGEAKVVPVPEEHPLEPVNVYGATKVAGEALVNSYRKAFGLRAWTLRLFN
VYGPSASPSRGVVGEFLRRALKGEPLRIYGDGRQVRDFVFVDDVVKAFKLVREIPEGTYNVGSGRGVSIITLAKKIIELT
GSKSEMVFLPERPGDVRVSVADVTKLAAFGWRPRVSLEEGLRLTAEALKARGT

Sequences:

>Translated_293_residues
MICITGGSGYIGSKLVEELLKEGEVKVLDLAPPPVPHVKFTRVNVLLLDDLKVELRDCELVYHLAAEIKAEESLREPAKV
VRVNVEGTLNVLEAARLADASVVFASTAAVYGEAKVVPVPEEHPLEPVNVYGATKVAGEALVNSYRKAFGLRAWTLRLFN
VYGPSASPSRGVVGEFLRRALKGEPLRIYGDGRQVRDFVFVDDVVKAFKLVREIPEGTYNVGSGRGVSIITLAKKIIELT
GSKSEMVFLPERPGDVRVSVADVTKLAAFGWRPRVSLEEGLRLTAEALKARGT
>Mature_293_residues
MICITGGSGYIGSKLVEELLKEGEVKVLDLAPPPVPHVKFTRVNVLLLDDLKVELRDCELVYHLAAEIKAEESLREPAKV
VRVNVEGTLNVLEAARLADASVVFASTAAVYGEAKVVPVPEEHPLEPVNVYGATKVAGEALVNSYRKAFGLRAWTLRLFN
VYGPSASPSRGVVGEFLRRALKGEPLRIYGDGRQVRDFVFVDDVVKAFKLVREIPEGTYNVGSGRGVSIITLAKKIIELT
GSKSEMVFLPERPGDVRVSVADVTKLAAFGWRPRVSLEEGLRLTAEALKARGT

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=297, Percent_Identity=34.006734006734, Blast_Score=151, Evalue=7e-37,
Organism=Homo sapiens, GI7657641, Length=315, Percent_Identity=29.2063492063492, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI56237023, Length=325, Percent_Identity=26.1538461538462, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI56118217, Length=325, Percent_Identity=26.1538461538462, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI189083684, Length=325, Percent_Identity=26.1538461538462, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI193211614, Length=219, Percent_Identity=29.2237442922374, Blast_Score=65, Evalue=9e-11,
Organism=Homo sapiens, GI8393516, Length=219, Percent_Identity=29.2237442922374, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI48994969, Length=330, Percent_Identity=29.3939393939394, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1788353, Length=334, Percent_Identity=28.7425149700599, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1786974, Length=322, Percent_Identity=28.8819875776398, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788365, Length=317, Percent_Identity=28.391167192429, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788589, Length=235, Percent_Identity=24.2553191489362, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17539532, Length=307, Percent_Identity=31.9218241042345, Blast_Score=138, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI115532424, Length=329, Percent_Identity=30.6990881458967, Blast_Score=104, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17568069, Length=322, Percent_Identity=28.5714285714286, Blast_Score=97, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI71982035, Length=329, Percent_Identity=27.6595744680851, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI71982038, Length=331, Percent_Identity=26.8882175226586, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6319493, Length=304, Percent_Identity=26.9736842105263, Blast_Score=95, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21356223, Length=300, Percent_Identity=31.6666666666667, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI19923002, Length=338, Percent_Identity=30.7692307692308, Blast_Score=103, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.2 [H]

Molecular weight: Translated: 31890; Mature: 31890

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MICITGGSGYIGSKLVEELLKEGEVKVLDLAPPPVPHVKFTRVNVLLLDDLKVELRDCEL
CEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEEEEEEEEECCEEEHHHHHH
VYHLAAEIKAEESLREPAKVVRVNVEGTLNVLEAARLADASVVFASTAAVYGEAKVVPVP
HHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCEEEEHHHHHCCCEEEEECC
EEHPLEPVNVYGATKVAGEALVNSYRKAFGLRAWTLRLFNVYGPSASPSRGVVGEFLRRA
CCCCCCCEEECCCHHHHHHHHHHHHHHHHCCHHEEEEEEEEECCCCCCCCCHHHHHHHHH
LKGEPLRIYGDGRQVRDFVFVDDVVKAFKLVREIPEGTYNVGSGRGVSIITLAKKIIELT
HCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHHC
GSKSEMVFLPERPGDVRVSVADVTKLAAFGWRPRVSLEEGLRLTAEALKARGT
CCCCCEEEECCCCCCEEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MICITGGSGYIGSKLVEELLKEGEVKVLDLAPPPVPHVKFTRVNVLLLDDLKVELRDCEL
CEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEEEEEEEEECCEEEHHHHHH
VYHLAAEIKAEESLREPAKVVRVNVEGTLNVLEAARLADASVVFASTAAVYGEAKVVPVP
HHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCEEEEHHHHHCCCEEEEECC
EEHPLEPVNVYGATKVAGEALVNSYRKAFGLRAWTLRLFNVYGPSASPSRGVVGEFLRRA
CCCCCCCEEECCCHHHHHHHHHHHHHHHHCCHHEEEEEEEEECCCCCCCCCHHHHHHHHH
LKGEPLRIYGDGRQVRDFVFVDDVVKAFKLVREIPEGTYNVGSGRGVSIITLAKKIIELT
HCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHHC
GSKSEMVFLPERPGDVRVSVADVTKLAAFGWRPRVSLEEGLRLTAEALKARGT
CCCCCEEEECCCCCCEEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]