Click here to switch to the map view.

The map label for this gene is cysS

Identifier: 156937781

GI number: 156937781

Start: 882317

End: 883711

Strand: Reverse

Name: cysS

Synonym: Igni_0991

Alternate gene names: 156937781

Gene position: 883711-882317 (Counterclockwise)

Preceding gene: 156937783

Following gene: 156937780

Centisome position: 68.11

GC content: 53.12

Gene sequence:

>1395_bases
TTGCTGAAGGTCTTCAACACGTTAACTAGGAGGTTAGAGGACTTCAAGCCTTTGGAGCCCCCTCTGGTTAGGATGTACGT
CTGCGGTCCGACGGTTTACGACCACTCTCACATAGGCCACGCGAGGACTTGGGTGGCGTTCGACATAATTAAAAAATACT
TAAGGTTGAAGGGTTATAACGTGATCCACGTTCAGAACATCACCGACATAGACGACAAGATAATAAACAGGGCGAGGGAG
CTCGGCGTCAGCTGGAAGGAGGTAGCGGACACCTACACGAACGAGTACTTCGAGCTCATGAAAAAACTAAAGGTCTTCCC
CACCGTCCACCCGAGGGTCACAGATCATATAGACGACATAATAGAGTTCGTTCAAGACTTGGTGGACAAGGGCTACGCGT
ACGTCACCCAGAGCGGGGTCTACTTCGACGTCGACAAGTACCCCTACTACGGCCAGCTCTCCGGGATAAAGGACAAGAAG
ATGTGGAGTCAAGAGGAGTTCGTCAAGGACAAGAAGAACCCGTACGACTTCGCCTTATGGAAGTGCGCCAAGCCCGGAGA
ACCTTGGTGGGACAGCCCTTGGTGTAAGGGCAGGCCGGGGTGGCACATAGAGTGCTCCACTATGAGTAGCAAATACTTGG
GTAAGCAGTTCGACGTTCACGGCGGCGCTAGGGACTTGATCTTCCCGCACCACGAAAATGAAATAGCTCAGAGCGAGGCT
CGCTTCGGCGTCAGGCCTTGGGTGAAGTACTGGCTCCACTCCGGCTACTTGACCGTCAAAGGGGAGAAGATGAGTAAAAG
TTTGGGCAACATAGTGCCGTTGAAGGACGTGTTGAAGAGCTTCGAGCCGGAAGTAGTTAGGTACTGGCTGTCGTCCGCCC
ACTACCGCACGGAGCTGGACTTCTCGTGGGAGAGGCTAGAAGAGGCCAAGAGGAGCTTAACTAGGATGAGAATGACCGTC
GACGAGTTGAGGAAGATAGTTAAAAAGGAGAGTCCAAAGGGCAAGCTGGACGAGTGGGAAATAAAGGCCATTTACGAAGT
CTCGAAGATTAGGAATGAGTTTTACGACGCTATGGACAACGACTTCAACACCCCCGAAGCGTGGGCGGCCGTGAGGGAGG
CGTTGAGGTTGGGCAACAAGGCCATAGAAGAGGGGAGTTGGGAAGTCTCCTTAGCCGTCTTAGAGTTCGTCAACGAGGCC
GACAAGGTCTTTGAGGTCTTCGAAGAGAGGGTCCACGAAGGGGTTGAGCCGTTCATAGACTTGCTAGTTGAAGTGAGGAG
CAAGCTGAGGGAGATGAAACAGTGGGAGCTGGCCGACTATATAAGGTCGAAGCTGGACGAGCTCGGGGTCAAGCTGTTGG
ACAAGGGCAAGGAAACGGAGTGGCGTTTCGCGTGA

Upstream 100 bases:

>100_bases
GCCTTACGAGCATTTTTTCCATCCCATGAGGGGCGCGGAGGCGAAGTTATTTTGTTGAAGCCCCATTAAGCGCCGCCCCC
TTCTCGCGAGGGGTCCACCC

Downstream 100 bases:

>100_bases
TGAAACCTTGGCCGGGCGATCGGTGAGCGGATAGGTCACGGGGCCTGAACCCCTTAAGCATTAACCCTTTCCTCGCTCGC
CTCGGACGCATGAAGGAACT

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MLKVFNTLTRRLEDFKPLEPPLVRMYVCGPTVYDHSHIGHARTWVAFDIIKKYLRLKGYNVIHVQNITDIDDKIINRARE
LGVSWKEVADTYTNEYFELMKKLKVFPTVHPRVTDHIDDIIEFVQDLVDKGYAYVTQSGVYFDVDKYPYYGQLSGIKDKK
MWSQEEFVKDKKNPYDFALWKCAKPGEPWWDSPWCKGRPGWHIECSTMSSKYLGKQFDVHGGARDLIFPHHENEIAQSEA
RFGVRPWVKYWLHSGYLTVKGEKMSKSLGNIVPLKDVLKSFEPEVVRYWLSSAHYRTELDFSWERLEEAKRSLTRMRMTV
DELRKIVKKESPKGKLDEWEIKAIYEVSKIRNEFYDAMDNDFNTPEAWAAVREALRLGNKAIEEGSWEVSLAVLEFVNEA
DKVFEVFEERVHEGVEPFIDLLVEVRSKLREMKQWELADYIRSKLDELGVKLLDKGKETEWRFA

Sequences:

>Translated_464_residues
MLKVFNTLTRRLEDFKPLEPPLVRMYVCGPTVYDHSHIGHARTWVAFDIIKKYLRLKGYNVIHVQNITDIDDKIINRARE
LGVSWKEVADTYTNEYFELMKKLKVFPTVHPRVTDHIDDIIEFVQDLVDKGYAYVTQSGVYFDVDKYPYYGQLSGIKDKK
MWSQEEFVKDKKNPYDFALWKCAKPGEPWWDSPWCKGRPGWHIECSTMSSKYLGKQFDVHGGARDLIFPHHENEIAQSEA
RFGVRPWVKYWLHSGYLTVKGEKMSKSLGNIVPLKDVLKSFEPEVVRYWLSSAHYRTELDFSWERLEEAKRSLTRMRMTV
DELRKIVKKESPKGKLDEWEIKAIYEVSKIRNEFYDAMDNDFNTPEAWAAVREALRLGNKAIEEGSWEVSLAVLEFVNEA
DKVFEVFEERVHEGVEPFIDLLVEVRSKLREMKQWELADYIRSKLDELGVKLLDKGKETEWRFA
>Mature_464_residues
MLKVFNTLTRRLEDFKPLEPPLVRMYVCGPTVYDHSHIGHARTWVAFDIIKKYLRLKGYNVIHVQNITDIDDKIINRARE
LGVSWKEVADTYTNEYFELMKKLKVFPTVHPRVTDHIDDIIEFVQDLVDKGYAYVTQSGVYFDVDKYPYYGQLSGIKDKK
MWSQEEFVKDKKNPYDFALWKCAKPGEPWWDSPWCKGRPGWHIECSTMSSKYLGKQFDVHGGARDLIFPHHENEIAQSEA
RFGVRPWVKYWLHSGYLTVKGEKMSKSLGNIVPLKDVLKSFEPEVVRYWLSSAHYRTELDFSWERLEEAKRSLTRMRMTV
DELRKIVKKESPKGKLDEWEIKAIYEVSKIRNEFYDAMDNDFNTPEAWAAVREALRLGNKAIEEGSWEVSLAVLEFVNEA
DKVFEVFEERVHEGVEPFIDLLVEVRSKLREMKQWELADYIRSKLDELGVKLLDKGKETEWRFA

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=387, Percent_Identity=41.343669250646, Blast_Score=287, Evalue=1e-77,
Organism=Homo sapiens, GI10835051, Length=412, Percent_Identity=34.2233009708738, Blast_Score=208, Evalue=9e-54,
Organism=Homo sapiens, GI62240992, Length=412, Percent_Identity=34.2233009708738, Blast_Score=208, Evalue=1e-53,
Organism=Homo sapiens, GI303304987, Length=412, Percent_Identity=34.2233009708738, Blast_Score=208, Evalue=1e-53,
Organism=Homo sapiens, GI21269877, Length=412, Percent_Identity=34.2233009708738, Blast_Score=208, Evalue=1e-53,
Organism=Escherichia coli, GI1786737, Length=475, Percent_Identity=44.421052631579, Blast_Score=370, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17509797, Length=440, Percent_Identity=32.5, Blast_Score=209, Evalue=4e-54,
Organism=Caenorhabditis elegans, GI17509795, Length=440, Percent_Identity=32.5, Blast_Score=208, Evalue=4e-54,
Organism=Saccharomyces cerevisiae, GI6324082, Length=303, Percent_Identity=39.6039603960396, Blast_Score=218, Evalue=2e-57,
Organism=Drosophila melanogaster, GI19922218, Length=485, Percent_Identity=35.8762886597938, Blast_Score=287, Evalue=1e-77,
Organism=Drosophila melanogaster, GI19922364, Length=416, Percent_Identity=32.9326923076923, Blast_Score=219, Evalue=4e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_IGNH4 (A8AB68)

Other databases:

- EMBL:   CP000816
- RefSeq:   YP_001435577.1
- ProteinModelPortal:   A8AB68
- SMR:   A8AB68
- STRING:   A8AB68
- GeneID:   5563070
- GenomeReviews:   CP000816_GR
- KEGG:   iho:Igni_0991
- eggNOG:   arNOG04368
- HOGENOM:   HBG327651
- OMA:   VVKIYCC
- BioCyc:   IHOS453591:IGNI_0991-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00041_A
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 54721; Mature: 54721

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 266-266

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKVFNTLTRRLEDFKPLEPPLVRMYVCGPTVYDHSHIGHARTWVAFDIIKKYLRLKGYN
CCHHHHHHHHHHHHCCCCCCCCEEEEEECCCEECCCCCCCHHHHHHHHHHHHHHHHCCCC
VIHVQNITDIDDKIINRARELGVSWKEVADTYTNEYFELMKKLKVFPTVHPRVTDHIDDI
EEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
IEFVQDLVDKGYAYVTQSGVYFDVDKYPYYGQLSGIKDKKMWSQEEFVKDKKNPYDFALW
HHHHHHHHHCCCEEEECCCEEEECCCCCCCCCCCCCCHHHHCCHHHHHHCCCCCCCCCEE
KCAKPGEPWWDSPWCKGRPGWHIECSTMSSKYLGKQFDVHGGARDLIFPHHENEIAQSEA
EECCCCCCCCCCCCCCCCCCCEEEECCCHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHH
RFGVRPWVKYWLHSGYLTVKGEKMSKSLGNIVPLKDVLKSFEPEVVRYWLSSAHYRTELD
HCCHHHHHHHHHHCCEEEEECHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHCCCEEECC
FSWERLEEAKRSLTRMRMTVDELRKIVKKESPKGKLDEWEIKAIYEVSKIRNEFYDAMDN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
DFNTPEAWAAVREALRLGNKAIEEGSWEVSLAVLEFVNEADKVFEVFEERVHEGVEPFID
CCCCHHHHHHHHHHHHHCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
LLVEVRSKLREMKQWELADYIRSKLDELGVKLLDKGKETEWRFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MLKVFNTLTRRLEDFKPLEPPLVRMYVCGPTVYDHSHIGHARTWVAFDIIKKYLRLKGYN
CCHHHHHHHHHHHHCCCCCCCCEEEEEECCCEECCCCCCCHHHHHHHHHHHHHHHHCCCC
VIHVQNITDIDDKIINRARELGVSWKEVADTYTNEYFELMKKLKVFPTVHPRVTDHIDDI
EEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
IEFVQDLVDKGYAYVTQSGVYFDVDKYPYYGQLSGIKDKKMWSQEEFVKDKKNPYDFALW
HHHHHHHHHCCCEEEECCCEEEECCCCCCCCCCCCCCHHHHCCHHHHHHCCCCCCCCCEE
KCAKPGEPWWDSPWCKGRPGWHIECSTMSSKYLGKQFDVHGGARDLIFPHHENEIAQSEA
EECCCCCCCCCCCCCCCCCCCEEEECCCHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHH
RFGVRPWVKYWLHSGYLTVKGEKMSKSLGNIVPLKDVLKSFEPEVVRYWLSSAHYRTELD
HCCHHHHHHHHHHCCEEEEECHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHCCCEEECC
FSWERLEEAKRSLTRMRMTVDELRKIVKKESPKGKLDEWEIKAIYEVSKIRNEFYDAMDN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
DFNTPEAWAAVREALRLGNKAIEEGSWEVSLAVLEFVNEADKVFEVFEERVHEGVEPFID
CCCCHHHHHHHHHHHHHCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
LLVEVRSKLREMKQWELADYIRSKLDELGVKLLDKGKETEWRFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA